Republic of the Philippines

DAVAO DEL NORTE STATE COLLEGE

New Visayas, Panabo City

Name: __________________________
Noli B. Cajes Rating: ____________________
Date Performed: __________________
May 18, 2022

Laboratory Activity 6
Aseptic Techniques, Basic Laboratory Techniques for Isolation

Materials and Procedure (Please refer to the link below)

https://www.youtube.com/watch?v=v0G8Hd6R-14

Questions
1. Why is the aseptic technique important?

It is important because it is used to transfer bacteria from one place to another without
contaminating ourselves or the culture. Hence, it is essential to avoid contamination of
the culture.

2. What is the purpose of flaming the loop before use? After use?

Flaming before use kills any bacteria on the loop that might contaminate your culture.
Flaming after use kills any bacteria left on the loop from your bacterial transfer activities.

3. Why must the loop be cooled before touching it to a culture? Should you set it down
to let it cool? How do you determine when it is cooled?

It must cool because if it isn't the heat will kill the bacteria in the culture. You shouldn't
set down the loop to let it cool as it will be exposed to contaminants. It will no longer be
red, wait 2 minutes.

4. When is a loop preferable for transferring bacteria? When is a needle preferable?

It is preferable to use a loop for transferring bacteria when transferring from a broth to
a slant culture. While on the other hand, it is preferable to use a needle when using a
deep culture.
5. Generalize how to aseptically transfer bacteria from one form of culture medium to
another.
First, sterilize the loop by holding the wire in the flame until it is red-hot. Second, while
holding the sterile loop and the bacterial culture, remove the cap of the test tube. Third,
briefly heat the mouth of the tube in the flame before inserting the loop for an inoculum.
Fourth and last, get a loopful of culture, heat the mouth of the tube, and replace the
cap.

Conclusion

The aseptic technique is utilized for a range of procedures including culture

transfer, medium inoculation, pure culture isolation, and microbiological testing. The
proper aseptic approach protects cultures against contamination by foreign
microorganisms found in the environment.
Moreover, the aseptic technique can be employed through the following steps:
First, sterilize the loop by holding the wire in the flame until it is red-hot. Second, while
holding the sterile loop and the bacterial culture, remove the cap of the test tube. Third,
briefly heat the mouth of the tube in the flame before inserting the loop for an inoculum.
Fourth and last, get a loopful of culture, heat the mouth of the tube, and replace the cap.

Furthermore, Broth cultures contain a huge number of germs in a small area that
can be easily moved. Agar slants, like Petri plates, provide a stable growth surface,
however, slants are more convenient to store and transport. To make an agar deep, agar
is allowed to congeal in the bottom of the test tube. Deeps are frequently employed to
grow bacteria that demand less oxygen than the medium's surface.