CD Bioparticles

Provide one-stop customization service of gene

therapy for academic and industrial customers.
Gene therapy is developing into precise medicines that can manipulate specific genes in the treatment
of serious diseases and vaccines development. To effectively function in vivo, reliable delivery systems
are very important to the nucleic acids for protecting them from degradation and allowing effective
cellular uptake and release. Based on profound scientific research accumulation, strong R&D strength
and continuous technical support, CD Bioparticles could provide customers with one-stop
customization service as:

Nucleic acid services Delivery systems development Characterization

• Design • Polymer-based nanoparticles • Physical characterization

• Synthesis • Liposomes • Cell characterization
• Modifications • Cell-based nanoparticles • Animal studies
• Viral Vectors

Nucleic acid services platform

Design

According to the name of the disease or the gene, search the database and literature for the
sequence of the nucleic acid, and select the proper CDS for synthesis.

Synthesis

• In Vitro Transcription (IVT)

• Chemical synthesis for siRNA, mRNA, DNA, plasmid, ASO, sgRNA, shRNA
• The corresponding QC testing services

Modifications

5`-Terminal 3`-Terminal Code Region:

Cap0/ Cap1/Cap2, Poly(A) synthesis, UTR m1ψ, m5C, 2'-OMe,

Fluorescent Cap, modification… Pseudo-Uridine, LNA, N6

IRES/MS2, methyladenine, 5

UTR modification… methylcytidune…

* Other modifications if needed.

Delivery system development platforms

Polymer-based nanoparticles

Due to the wide range of sources and low immunogenicity, cationic polymers, such as PEI, dendrimers,
chitosan, gelatin are widely used nanoscales delivery systems for gene therapy.

Liposomes

With the similar structure of cell membrane, liposomes are considered to be a safer and more
effective delivery system. Its flexibility and robustness in lipid structure, composition, ratio and
preparation methods make liposomes an important artificial carrier for gene therapy. Targeting
ligands, such as peptides, antibodies have been successfully applied in liposomes to achieve
active targeting delivery to specified area. Furthermore, encapsulating fluorescent dyes in liposomes
is very beneficial for later tracking and imaging studies.

Lipid nanoparticles (LNPs) LNPs with target groups

• High nucleic acid loading • Active targeting delivery

• Stable in vivo • Tissue/cell selectivity

• Low toxicity • High bioavailability

Commercial LNPs Fluorescent liposomes

• SM-102/MC3/ALC-0315 for research only • Biological tracer

• FDA approved • Visual distinction

• Ideal delivery model

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Delivery system development platforms

Cell-based nanoparticles

Exosomes are nanovesicles derived from cell membrane with natural targeting ability. CD Bioparticles
provides various exosome production custom services for gene therapy research.

Viral vectors

Viral vectors are a tool commonly used in molecular biology to bring genetic material into cells. The
principle is to use the molecular mechanism of viruses to transmit their genomes into other cells for
infection. Its high effectiveness and tropism make viral vectors one of the most commonly used
carriers for gene therapy. CD Bioparticles could provide a wild range of viral vectors for basic
research and preclinical study.

Adenoviral vectors (Ads) Adeno-associated viral vectors (AAVs)

• Non-integrating • Non-integrating

• Large packaging capacity • Tissue tropisms

• Broad tropism • Various mutants

• Transduce (non-)dividing cells • Low immunogenicity

• High level expression • High level and long-term

Lentiviral vectors (LVs) Retroviral vectors (RVs)

• Integrating • Integrating

• Stable expression • Transduce dividing cells

• Long-term expression

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Characterization Platform

Physical characterization

• DNA sequencing
• Off-target detection
• Size & PDI
• Morphology
• Zeta potential
• Lipid assay (liposomes only)
• Encapsulation efficiency & Drug loading

Cell characterization

• Cell culture
• Cell targeting
• Gene & protein expression
• Titer definition
• Functional evaluation
• Pseudovirus & plasmids creation (viral vectors only)
• Viral vectors purification (viral vectors only)

Animal studies

• Animal model establishment

• Immunizations
• Safety evaluation
• Effectiveness evaluation
 Contact Us
Phone: 1-631-624-4882 (USA) / 44-161-818-6441 (Europe) / Fax:1-631-938-8221
E-mail: info@cd-bioparticles.com / www.cd-bioparticles.net
45-1 Ramsey Road, Shirley, NY 11967, USA