PLATELET

HISTOGRAM
Noer Hafni
• Histograms are graphic representation of cell frequencies verses size.
• Histogram provide information about erythrocytes ,leukocytes and
platelet frequency and distribution as well as presence of
subpopulation.
• Shift in one direction or another can be of diagnostic importance.
• Produced from thousands/millions of signals generated by the cells
passing through detector where they are differentiated by:
• Their size
• Frequency of occurrence in the population
 RBC and Platelet Histograms

The black line represents normal cell distribution. The red line on the RBC histogram
graphically represents a Microcytic red cell population.
Discrimation thresholds
• Platelet → volume : 8 – 12 fl counted from 2 – 30 fl
• RBC → volume : 80- 100 fl detected from
30 – 250 fl
• WBC → RBC are lysed by lytic reagent
different WBC discriminator set at different
levels between range 30 – 450 fl
HISTOGRAM TROMBOSIT
• Hitung trombosit antara 2 – 20 fL
Hitung trombosit : 2 – 20 fl
< 2 fl interference
• ➔Debu
• ➔Partikel-partikel EDTA
• →Air Babbles
• → Electronic and Electrical noise

> 20 fL interference
➔Fragment RBCs , Microcyte,
Scishtocyte
➔Fragment WBCs
→ Giant Plts, Clumped plts
 HISTOGRAM
TROMBOSIT
2 diskriminator fleksibel yang digunakan :
1. LD (Lower Discriminator)
• ➔dinamakan PL (Platelet LD)
• ➔diskriminator fleksibel yang
digunakan 2 – 6 fL
2. UD (Upper Discriminator)
• ➔dinamakan PU (Platelet UD)
• ➔diskriminator fleksibel yang
digunakan 12 – 30 fL
Diskriminator ke tiga yang fix digunakan
yaitu pada 12 fL
HISTOGRAM
TROMBOSIT

Kurva histogram trombosit

seharusnya berada dalam
LD dan UD
Normalnya kurva trombosit
condong ke kiri
Kurva Histogram trombosit
dimulai dan berakhir di
baseline
 HISTOGRAM
TROMBOSIT
MPV adalah
pengukuran dari rerata
volume hitung trombosit
(analoginya MCV
eritrosit )

Range referensi nya

adalah : 8 – 12 fL
 HISTOGRAM
TROMBOSIT
➔Variasi range ukuran
trombosit (MPV)
berdasarkan hitung
trombosit
➔Pada pasien normal,
MPV mempunyai
hubungan terbalik dengan
hitung trombosit
(↑ MPV dapat terlihat
pada trombositpenia )
PDW
Giant Platelets
Small Platelets
Sysmex XT 2000i
Sysmex XT 2000i
New parameter
Thank you