APPARATUS

No. 1 filter paper), jar cover and a fine capillary tik

Gas jar, glass rod, filter paper strip (Whatman be

REQUIREMENT
A mixture of red and blue inks, alcohol and,distilled water.
O d
3pin eovA, Ocatone, eth
PROCEDURE
with pencil above 3 cm from or.
Take a strip (20 x 2 cm) and draw a line
Whatman filter paper one
the of the as shown in Fig. 6.4.
end. Draw another line lengthwise from centre paper

Chromatographic
paper

Original line about

4 cm above the edge

P
Point to put the
spot of the mixture

Fig. 6.4. Spotting of the mixture.

d leaves Sanpie
2.
CgLLhe
With the help of fine capillary tube, put a drop of the mixture of redand bleeinms at the po
P. Let it dry in air. Put another drop on the same spot and dry again. Repeat 2-3 times, sotha
the spot is rich in the mixture.
3. Suspend the filter paper vertically in a gas jar containing the solvent (eluent) with the helpot
glass rod in such a way that the pencil line (and the spot) remains about 2 cm above the solven
level (50%-aleohoB+distiHed-water).
4. Cover the jar and keep it undisturbed. Notice the rising solvent along with the mixture ot e
andblue inks. After the solvent has risen about 15 cm you will notice two different spots ot Du

and red colours on the filter paper.

5. Take the flter paper out ofthe jar and mark the distance that the solvent has risen on the pap
with a pencil. This is called the solvent front. qrun plew
6. Dry the paper. Put pencil marks in the centre of the blueand-red spots.
from
7. Measure the distance of the two spots from the original line and the distance ofthe solvent
the original line.
 57
chromatography

Calculate the R, values of the blue and red inks by using the formula:
Yeou7rter sp01
Distance travelled by the blue or red imk from the point of application

R Distance travelled by the solvent from the original line

PRECAUTIONS dissolve in the

that the mark does
not
for drawing the reference line so

V1. Use good quality pencil

carried out.
the chromatography is
solvent in which
capillary tube.
of a fine
Always make
use
2.
the experiment.
undisturbed and covered during
3. Keep the jar
rich in mixture.
should be small and
4. A spot
the strip in the jar.
5. Allow the spot
to dry before putting
let it to be curled.
erect. Do not
6. Keep the strip
solvent.
the spot to dip in the
7. Do not allow

EXPERIMENT 6.2

theegivengrass/flower by ascending paper

components present in
the coloured
To separate their R,values.
AIM and d e t e r m i n e the juice with
acetone. Use this
chromatography m o r t a r and
extract
in a
crush fresh flowers orgrass
In this experiment,
solution for making the spot.

Proceed as in Expt. 6.1.

OBSERVATIONS AND CALCULATIONS
Distance travelled by
Distance travelled
the R,Value
the solvent from
spot from the original line
Colour of the spot by the
original line A/X
cm
A cm
Green B/X
X cm
(Chlorophyl) B cm
Yellow
X cm
Xanthophyl)
Ccm
Red
(Carotene)