Experiment No 1

Food sample analysis

AIM: To detect the presence of carbohydrates, fats and proteins in the given foodstuff.

APPARATUS: Test tubes, beakers, glass rod, droppers, filter paper, burner.

CHEMICALS: Fehling solution A & B, iodine solution, NaOH solution, 1% CuSO4 solution.

S. No. TEST OBSERVATION INFERENCE

1 Test for carbohydrates

(Fehling’s Test) No red precipitate is Carbohydrates are absent.
Take 1 ml Sample + 1 ml each formed.
of Fehling’s solution A and B in
a test tube. Keep the test tube in
a boiling water bath for 5 mins.

2 Test for starch (Iodine Test)

Sample + 1 drop of Iodine Blue colour appears Starch is present.
solution

3 Test for proteins (Biuret Test)

Sample + 2 ml NaOH solution + No bluish violet colour Proteins are absent.
4-5 drops of 1% CuSO4 solution. obtained.
Warm the mixture in a water
bath for 5 mins.

4 Test for fats ( Translucent Spot

Test)
Place a drop of sample on a filter No greasy or translucent Fats are absent.
paper and let it dry. spot on the filter paper.

RESULT: The given foodstuff contains starch.

PRECAUTIONS:
1. Use freshly prepared reagents for performing tests.
2. Use minimum quantities of reagents.
 Experiment No 2
Food sample analysis

AIM: To detect the presence of carbohydrates, fats and proteins in the given foodstuff.

APPARATUS: Test tubes, beakers, glass rod, droppers, filter paper, burner.

CHEMICALS: Fehling solution A & B, iodine solution, NaOH solution, 1% CuSO4 solution.

S. No. TEST OBSERVATION INFERENCE

1 Test for carbohydrates

(Fehling’s Test) Red precipitate is formed. Carbohydrates present.
Take 1 ml Sample + 1 ml each
of Fehling’s solution A and B in
a test tube. Keep the test tube in
a boiling water bath for 5 mins.

2 Test for starch (Iodine Test)

Sample + 1 drop of Iodine No blue colour obtained Starch is absent.
solution

3 Test for proteins (Biuret Test)

Sample + 2 ml NaOH solution + No bluish violet colour Proteins are absent.
4-5 drops of 1% CuSO4 solution. obtained.
Warm the mixture in a water
bath for 5 mins.

4 Test for fats ( Translucent Spot l

Test)
Place a drop of sample on a filter No greasy or translucent Fats are absent.
paper and let it dry. spot on the filter paper.

RESULT: The given foodstuff contains carbohydrates. .

PRECAUTIONS:
3. Use freshly prepared reagents for performing tests.
4. Use minimum quantities of reagents.