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NEAR EAST UNIVERSITY

Faculty of Pharmacy
Department of Analytical Chemistry

Analytical Chemistry Lab. II

Assist. Prof. Dr. Usama Alshana

2020-2021
TRNC

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Experiment 3: Column Chromatography

POST-LAB REPORT

Results and Discussion

1. Preparation of chromatography column

Draw the apparatus used in this experiment and label its components

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2. Loading the sample and separating the analytes

(a) Which substance was eluted first and which one was eluted next from the column?

(b) Explain why these two analytes were eluted in this order.

Questions
In the chromatogram shown below, calculate the following:

(a)

(b)

(c)

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(d)

(e)

(f)

(g)

(h)

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