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Effects of Pruning, Staking and Different Fungicide Frequencies on Late Blight


Severity and Fruit Yield of Tomato in Dschang, West Region of Cameroon

Article · January 2021

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Scholarly Journal of Agricultural Science Vol. 11(1), pp. 1-7 January, 2021
Available online at http:// www.scholarly-journals.com/SJAS
ISSN 2276-7118 © 2021 Scholarly-Journals

Full Length Research Paper

Effects of Pruning, Staking and Different Fungicide


Frequencies on Late Blight Severity and Fruit Yield of
Tomato in Dschang, West Region of Cameroon
Tabi N. Gaetan*1, Divine N. Tarla1, Nelson N. Ntonifor 2 and Nerlus G. Sopkoutie2
1
Plant Pathology and Agricultural Zoology Research Unit, Department of Plant Protection, Faculty of Agronomy
and Agricultural Sciences (FASA), University of Dschang, P.O Box 222, West Region, Cameroon.
2
Department of Agronomic and Applied Molecular Sciences, Faculty of Agriculture and Veterinary Medicine,
University of Buea, P. O Box 63, South West Region, Cameroon.

Received: December 4, 2020, Accepted: January, 8, 2020, Published: January 25, 2021

Tomato (Solanum lycopersicon L.) an important vegetable crop used in almost every meal in all homes
and restaurants to create appetite and taste. It has the potential of becoming a corner stone in reducing
chronic hunger and alleviating poverty of small holder farmers in Cameroon especially in the Western
highlands but its production results in low yields due to small and/or rotten fruits at harvest. The yields
can be increased if farmers adopt good agronomic practices. This research was therefore carried out to
determine the effects of staking, pruning and different fungicide frequencies on late blight severity and
fruit yield of tomato. The Cobra tomato variety was planted in a Randomized Complete Block Design
experiment with four treatments and four replications each. Plant height, number of days to flowering,
number of flowers and fruits per plant, number of diseased fruits, disease severity, fruit weight and
total yield were recorded. The results showed a 100% disease incidence in all the treatments, whereas
disease severity was significantly higher in treatments that were not staked compared to those that
were staked. Pruning significantly reduced plant height and number of flowers per plant. Staking did
not cause any significant differences in plant height, number of flowers and number of fruits among
treatments. At harvest, plants that were not staked and not pruned and those that were staked and not
pruned had higher number of fruits with smaller sizes, compared to plants that were staked and pruned
and those that were not staked and pruned which had fewer but bigger fruits per plant. The study
concluded that staking and pruning together with the application of fungicides at the rate of one time
per week was appropriate for producing clean and bigger fruits with an increase in total marketable
fruit yield by weight. It is therefore recommended that tomato farmers should adopt staking and
pruning together with the judicious use of fungicides to control late blight and to obtain higher
marketable yields that will fetch good prices and safe for human consumption.

Key words: Tomatoes, Pruning, Staking, Fungicide, Disease severity, Late blight, Yield.

INTRODUCTION

Tomato (Solanum lycopersicon L.) is one of the main processed and it is a very good source of vitamins A, B,
open field vegetables cultivated and consumed in C and minerals (Sallam et al., 2012). It is the world's
Cameroon. It is of great dietary and economic second most cultivated crop, with a production estimated
importance. Tomato is one of the most remunerable and at about 182,301,395 tons. China is the world’s largest
widely grown vegetables in the world. It is grown for its producer (59,626,900 tons) followed by India (20,708,000
edible fruits, which can be consumed either fresh or tons). Tomato production in Cameroon is estimated at
1,279,853 tons with an average yield of 12,124.3 kg ha-1
(FAOSTAT, 2017). This is much lower than the mean
world yield of 37,600.4 kg ha-1 (FAOSTAT, 2017). Such
*Corresponding author E-mail: gaetantabi182@gmail.com. low yields may be due to the inefficient production
Scholarly J. Agric. Sci. 2

practices of farmers including their inability to fully exploit Peasant tomato farmers (both small and large scale)
available technologies and techniques coupled with spend huge sums of money yearly to purchase chemical
limited factors of production making it difficult for farmers pesticides making the production of the crop not very
to increase production through the use of more inputs profitable. These chemical fungicides are also not
(Kavoi et al., 2016). environmentally friendly since they have adverse effects
Late blight is the most challenging plant disease to on human health and other non-target organisms.
control, the major economic host crops, potato (Solanum Indeed, tomato profit margins and total production could
tuberosum L.) and tomato are considered important increase significantly if smaller amounts of chemicals are
horticultural crops in several countries severely affected used rationally in combination with good horticultural
by the disease (Fontem, 2004). The causal agent of late practices such as staking and pruning.
blight is the oomycetes, Phytophthora infestans (Mont.) This study was therefore designed to assess the
De Bary, well known for its explosive development when effects of staking, pruning and different fungicide
environmental conditions are suitable and when host frequencies on late blight severity and fruit yield of
plants are susceptible to infection (Mizubuti and Fry tomato.
2006). Complete field destruction due to late blight
epidemics have been observed (Fontem, 2003).
Worldwide economic losses due to the disease vary from MATERIALS AND METHODS
3 to 5 billion dollars annually (Halder et al., 2006). What
makes P. infestans an aggressive pathogen and late Location of the study site
blight such a difficult disease to manage is because the
pathogen has virulent genes that allow rapid infection and The study was conducted at the teaching and research
host tissue colonization (Halder et al., 2006). Despite the farm of the Faculty of Agronomy and Agricultural
achievements in understanding the biology of P. sciences of the University of Dschang. Dschang is
infestans, the molecular mechanisms involved in the located on latitudes 5 ! 25! North and longitude 10!04! East
pathogenesis are not yet completely resolved (Lee et al., and 1340m above sea level. Temperatures range from
0 0
2006). Also, the latency period is as short as three days 20 C to 28 C with annual rainfall between 3000mm to
which may give rise to many spores. Thus, there is 5000mm. It has the Tropical climate type with a
enormous interest in finding effective non-chemical monomodal rainfall pattern, characterized by a short dry
alternatives to protect tomato fields against this season from December to February and a long rainy
devastating disease. season from March to November. The mean annual
Late bight management has been based on the heavy average rainfall ranges between 1800 mm and 2000 mm
use of fungicides in many areas. Fungicide application while the mean annual average temperature is between
has increased over the last decade due to the 25°C and 28°C. Average insolation is 4.75 hours/day.
-
introduction of new and more aggressive genotypes of Dschang has a well drained ferralitic (Oxisol) type which
the pathogen (Kato et al., 1997). Organic production of is acid soil and rich in organic matter but poor in
tomato has for many decades depended on the use of phosphorus and potassium (Fongang 2008).
copper-based fungicides (copper per oxide, fixed-copper
hydroxide and copper oxychloride) to control late blight. Land Preparation
However, there is an increasing pressure to find
substitutes for these products because of environmental A piece of land of 180m2 was used for the experiment.
contaminations caused by copper residues. Currently in The land was cleared, tilled and demarcated into 16 sub-
the European Union, only 6 kg of elemental copper plots of area 3m by 2m each with a distance of 0.5m
-1 -1
hactare year is allowed for organic production between sub-plots and 1m between blocks. After
(Ghorbani et al., 2004). establishment of experimental units, pegging was done to
In Cameroon, tomato growers usually apply 2 to 3 indicate the planting positions. The planting distance was
fungicidal sprays per week to limit early and late blight 75cm inter rows and 50cm intra rows, giving a total of
infections during the wet season (Fontem et al., 2004). 18plants per experimental unit. Poultry manure at the rate
Unfortunately, the intensive sprays cause adverse effects of 200g per hole of 15cm deep and 15cm wide was
on health and the environment. Seeds free of late blight applied. The manure was watered for three (3) days to
pathogens are not easily available to farmers and this enable complete decomposition and heat release.
poses a great problem. Rogueing of infected plants from
fields is also not well implemented by farmers; Experimental design, field layout and transplanting
destruction of infected debris of previous tomato crop,
use of resistant varieties, crop rotation, fallowing are The experiment was laid in a Randomized Complete
control strategies not often practiced by farmers who rely Block Design (RCBD) with four treatments as follows; T1
highly on fungicide applications (Mizubuti et al., 2007; = Staked and Pruned only; T2 = Staked and not Pruned
Maerere et al., 2010). with fungicide application once a week; T3 = Not staked
Tabi et al., 3

Table 1: Pesticides used in the study

Pesticides Commercial name Active ingredient Registration number Doses


Fungicides Manco-sam 80WP Mancozeb800g/kg 617/11/FO/HOMO/CNHPCAT/CMR 80-100g/ 16L
sprayer
FLASH ONE Chlorothalonil 30% + 979/16/FO/HOMO/CNHPCAT/CMR 22.5g/ 16L sprayer
Cymoxanil 6% WP
Insecticide TAPIR Chlorpyriphos-Ethyl 776/13/IN/HOMO/CNHPCAT/CMR 37.5ml/ 15L sprayer
600g/l

Table 2: Disease scale for rating of tomato late blight


Disease Rating Symptoms severity for whole plant assay Infection % age Disease reaction
0 No visible symptoms apparent 0 Immune
1 A few minute lessions to about 10% of the total leaf area is 0.01-10 Highly resistant
blighted and usually confined to the 2 bottom leaves.
2 Leaves on about 25% of the total plant are infected 10.01-25 Resistant
3 Leaves on about 50% of the total plant are infected 25.01-40 Moderately resistant
4 Leaves on about 75% of the total plant are infected 40.01-60 Susceptible
5 Leaves on whole plant are blighted and plant is dead >60 Highly susceptible
Source: Galanihe et al., (2004)

and Pruned with fungicide application once a week; T4 was done once a week in the early morning on a dry day
=Not staked and not Pruned with fungicides applied 2 in order to allow the pruning wounds heal cleanly and
times per week (Control). Seedlings were transplanted at also to reduce the chances of the plant being infected by
four (4) weeks after germination. Few hours before pathogens.
transplanting, the nursery beds were watered Bamboo stakes of about 2m high were inserted into the
appropriately in order to soften the soil and ease removal ground about 3-6 cm from the base of the tomato
of seedlings. The seedlings were then carefully removed seedling; precaution was taken to avoid root damage. A
from the soil to avoid damage of the roots and placed in length of string (rope) was then used to tie the stem or
their appropriate holes then covered with soil. After extended branch of the plant to the stake. The branches
transplanting, the plants were watered thoroughly to were tied to the stake opposite blossoms so that when
enable the plant’s roots to grow and settle properly into the fruit grows it is not trapped between the stake and the
the soil. Watering was done twice daily (morning and tie.
evening) until the plants were fully established. Ten days
after transplanting NPK (20:10:10) fertilizer was applied Data collection
at the rate of 5g per plant to boost growth and
development. Replacement of death plants and plants Four plants from the middle of each sub-plot were
with retarded growth was also done. selected and tagged for data collection throughout the
experiment. Data was collected once weekly on the
Application of various treatments following parameters; plant height, disease severity,
number of flowers per plant, number of fruits per plant,
Three (3) weeks after transplanting (WAT) when the number of diseased fruits per treatment, fruit weight and
plants had fully established, the various treatments of fruit yield. Plant height was measured from the base of
staking and pesticide applications were done. the plant to the apex using a tape at 4, 5 and 6, weeks
Pruning was done when the plants were about 25-50cm after transplanting (WAT).
tall since plants may not recover from the shock of Disease severity of late blight on leaves and stems
pruning below this height. A clean and sharp pair of were recorded at 6, 7and 8 WAT and that on fruits at 8
pruning scissors was used to remove small suckers off. and 9 WAT. The disease was graded on a scale of 0-5
Suckers are small shoots that grow out of the join where shown on the table below;
a branch on the tomato plants meets a stem. These small
shoots will grow into a full sized branch if not pruned, It was then calculated using the formula;
resulting in a bushier and more sprawling plant. Pruning
Scholarly J. Agric. Sci. 4

Table 3: Effects of staking and pruning on plant height.


Plant Height (cm)
Treatments 4WAT 5WAT 6WAT
T1 (Staked and Pruned) 21.91 ± 3.51 a 21.44± 3.40 b 33.34 ± 4.70 b
T2 (Staked but not pruned) 24.22 ± 0.57 a 32.19 ± 2.10 a 42.28 ± 2.09 a
T3 (Not staked and Pruned) 26.18 ± 4.01 a 22.13 ± 5.04 b 34.81 ± 4.97 b
T4 (Not staked and Not pruned) 25.56 ± 4.63 a 31.78± 5.44 a 42.72 ± 5.41 a
Columns with means having a common letter do not differ significantly (p>0.05)

Table 4: Effect of staking and pruning on number of days to flowering


Treatments Days to Flowering
T1 (Staked and Pruned) 38.95 ± 2.42 a
T2 (Staked and Not pruned) 37.21 ± 6.01 a
T3 (Not staked and Pruned) 39.01 ± 3.54 a
T4(Not staked and Not pruned) 37.25 ± 1.98 a
Columns with means having a common letter do not differ significantly (p>0.05)

The numbers of flowers per plant were counted at 50% Effects of staking and pruning on number of days to
and 100% flowering. Number of diseased plants per flowering
treatment was counted at 4, 5 and 6 WAT while the,
number of fruits per plant was at 7, 8, and 9 WAT. Treatments that were staked but not pruned and those
Number of diseased fruits per treatment was recorded on that were not pruned and not staked flowered earlier than
a 3 days interval at 8 and 9 WAT. Fruit weight was those of the other treatments, but the differences were
obtained by harvesting and weighing the fruits for each not significant. Staking did not have any effect on days to
treatment using a scale balance with Kg units. Fruits flowering. (Table 4).
were harvested every three days from tagged plants and
records for each plot were summed at the end of the Effects of staking and pruning on number of flowers
harvest to obtain the total fruit yield for each treatment per treatment
which was then converted to yield per hectare.
Plants from treatments that were not pruned had
Data Analysis significantly higher (p<0.05) number of flowers per plant
compared to the other treatments at 4, 5 and 6 WAT
The data collected was entered in Microsoft excel 2016. (Table 5).
The average values of the growth, disease severity and
yield variables were subjected to repeated measures of Effects of staking and pruning on number of fruits
analysis of variance (ANOVA) to evaluate the effects of per treatment
the different treatments and their interaction. The
analyses were done using the software R analytics There were significant differences (p <0.05) in numbers
Version 3.1.1. Means for different treatments were of fruits amongst treatments. At 6 weeks after
compared using the Least Significant difference (LSD) transplanting, the numbers of fruits were similar amongst
test at 5% threshold. Shapiro’s test was used to rank the all treatments but at 7, 8 and 9 WAT, treatments that
means. were not staked and not pruned had significantly higher
numbers of fruits (p<0.05) than those in treatments that
were staked and pruned and those that were not staked
RESULTS and pruned but similar to the staked and not pruned
(Table 6).
Effects of pruning and staking on plant height
Combined effects of staking, pruning and frequency
There were significant differences (p <0.05) in plant of fungicide application on numbers of diseased
height among treatments. At 4 weeks after transplanting fruits
(4 WAT), plant height was similar among all treatments
but at 5 and 6 WAT, treatments that were not staked and There were significant differences (p<0.05) in numbers of
not pruned- had significantly taller plants (p<0.05) than diseased fruits amongst treatments. At 9 to 13WAT,
staked and pruned, not staked and pruned, but similar to treatments that were staked and pruned but had no
staked and not pruned (Table 3) fungicide application and those that were staked but not
Tabi et al., 5

Table 5: Effect of staking and pruning on number of flowers per treatment


Treatments 4WAT 5WAT 6WAT
T1 (Staked and Pruned) 0.75 ± 0.74 b 2.88± 1.33 b 6.56 ± 1.34 b
T2 (Staked and Not pruned) 2.19 ± 1.25 a 6.25 ± 1.81 a 11.31 ± 1.70 a
T3 (Not staked and Pruned) 1.00 ± 0.20 ab 3.69 ± 0.77 b 7.56 ± 0.90 b
T4 (Not staked and Not pruned) 2.19 ± 0.83 a 6.38 ± 1.65 a 12.06 ± 1.91 a
Columns with means having a common letter do not differ significantly (p>0.05)

Table 6: Effects of staking and pruning on numbers of fruits per treatment


Treatments 6WAT 7WAT 8WAT 9WAT
T1 (Staked and Pruned) 4.19 ± 0.94 a 9.38 ± 1.83 b 14.25 ± 1.85 b 19.63 ± 2.29 b
T2 (Staked and Not pruned) 5.56 ± 2.90 a 14.06 ± 3.30 a 19.15 ± 2.74 a 26.00 ± 3.26 a
T3 (Not staked and Pruned) 4.06 ± 1.21 a 10.25 ± 2.52 b 15.50 ± 1.84 b 20.44 ± 1.56 b
T4 (Not staked and Not pruned) 4.69 ± 1.68 a 12.98 ± 1.76 a 17.94 ± 2.81 a 23.14 ± 3.24 ab
Columns with means having a common letter do not differ significantly (p>0.05)

Table 7: Combined effects of staking, pruning and frequency of fungicide application on number of diseased fruits per treatment
Treatments 9WAT 10WAT 11WAT 12WAT 13WAT
T1 (Staked and Pruned/F0) 2.31 ± 0.55 b 2.25 ± 0.43 b 3.44 ± 0.20 c 2.50 ± 0.52 b 3.63 ± 0.32 c
T2 (Staked and Not pruned/F1) 2.19 ± 0.31 b 1.88 ± 0.20 b 2.75 ± 0.24 b 1.94 ± 0.47 b 2.63 ± 0.48 b
T3 (Not staked and Pruned/F 1) 3.81 ± 0.32 a 3.56 ± 0.47 a 4.56 ± 0.43 a 3.38 ± 0.48 a 4.94 ± 0.31 a
T4 (Not staked and Not pruned/F2) 3.63 ± 0.32 a 3.06 ± 0.24 a 3.75 ± 0.71 b 3.25 ± 0.46 a 4.56 ± 0.52 a
Columns with means having a common letter do not differ significantly (p>0.05)

pruned and with one fungicide application per week had in treatments that were not pruned (produced many fruits)
significantly lower numbers of diseased fruits (p>0.05) than those that were pruned (produced fewer fruits).
than those of treatments that were not staked and pruned Staking did not have an influence on fruit weight.
but had one fungicide application as well as those that
were not staked and not pruned but with two fungicide
applications per week (Table 7). DISCUSSIONS

Combined effects of staking, pruning and fungicide Effects of staking and pruning on plant height
frequencies on late blight severity
Pruning negatively affected plant height; plants that were
There was no significant difference (p>0.05) between not staked and not pruned and those that were staked
treatments at 4, 5, 6 and 7 WAT; in contrast, at 8, 9 and and not pruned were taller most probably due to the fact
10, 11 and 12 WAT there were significant differences that the terminal buds (growing tips) were left to grow.
(p<0.05) in late blight severity on plants of different The type of pruning used in the study inhibited the apical
treatments. Plants in treatments that were staked and growth of the plants. Sinnaduiai (2000), reported that
pruned but with no fungicide application (T1) and those pruning of tomato plants and the type of pruning used
that were staked and not pruned but with one fungicide has an effect on plant height. Pruning affected plant
application per week (T2) had significantly lower height negative by inhibiting apical growth of plants.
percentage of plants with symptoms of late blight Staking did not have any effect on the plant height.
compared to plants in treatments that were not staked
and pruned but with one fungicide per week (T3) and Effects of staking and pruning on number of flowers
those that were not staked and not pruned but received and fruits
two fungicide applicaions per week (Table 8).
Number of flowers and fruits were affected by pruning
Combined effects of staking, pruning and different and staking; plants that were not staked and not pruned
fungicide frequencies on tomato fruit yields produced higher number of flowers and fruits per plant
similar to those that were staked and not pruned. This
Fruit weight was slightly higher in treatments that were higher number of flowers per plant could be attributed to
pruned (larger fruits) than those that were not pruned the higher number of branches on each of these plants.
(smaller fruits). Fruit yield was highly significant (p<0.05) This is in line with the works of Geisenberg and Ploeg
Scholarly J. Agric. Sci. 6

Table 8: Combined effects of staking, pruning and fungicide frequency on late blight disease severity on tomato plants

Treatments 7WAT 8WAT 9WAT 10WAT 11WAT 12WAT


T1 (Staked and 0.25 ± 0.20 a 0.88 ± 0.25 b 1.25 ± 0.20 c 1.94 ± 0.13 c 2.44 ± 0.43b 2.69 ± 0.38 c
Pruned/F 0)
T2 (Staked and Not 0.56 ± 0.13 a 1.19 ± 0.24 ab 1.63 ± 0.25 bc 2.38 ± 0.25 b 2.50 ± 1.50 b 3.63 ± 0.32 b
pruned/F1)
T3 (Not staked and 0.94 ± 0.13 a 1.50 ± 0.20 a 2.00 ± 00 a 2.88 ± 0.25 a 4.13 ± 0.14 a 4.75 ± 0.20 a
Pruned/F 1)
T4 (Not staked and 0.88 ± 0.25 a 1.31 ± 0.35 a 1.75 ± 0.29 ab 2.50 ± 0.46 ab 2.50 ± 1.35 b 4.13 ± 0.43 b
Not pruned/F 2)
Columns with means having a common letter do not differ significantly (p>0.05).

Table 9: Combined effects of Staking, Pruning and different fungicide Frequencies on tomato fruit yield

Treatments 9WAT 10WAT 11WAT 12WAT 13WAT Average Yield in tons/Ha


T1 (Staked and Pruned/F0) 17.5 30.8 38.3 26.7 41.7 31.0
T2 (Staked and Not pruned/F1) 19.2 33.3 45.8 28.3 46.7 34.7
T3 (Not staked and Pruned/F 1) 15.8 22.5 38.3 22.5 40.0 27.8
T4 (Not staked and Not pruned/F2) 20.8 37.5 48.3 27.5 45.0 35.8

N/B: Each value is the mean of four replicates

(2001) who reported that pruning affected the number of not staked and pruned with fungicides applied once per
flowers and fruits in tomatoes. Staking did not have any week as well as those that were not staked and not
effect on number of fruits and flowers. pruned with two rounds of fungicides a week.
Staking improves air circulation around the plants,
Effects of staking, pruning and different fungicide preventing the build-up of high relative humidity which
frequencies on number of diseased fruits favours the development of late blight (Quinn, 1974;
Kwapata 1990). It also prevents plants from being in
Plants that were staked and pruned with no fungicide direct contact with the soil which is considered a reservoir
application and those that were staked but not pruned for inoculum (Patterson, 1990). Pruning slightly reduced
with one fungicide application per week had significantly the risk of spreading the disease from one plant to
lower numbers of diseased fruits compared to plants that another.
were not staked and pruned with one fungicide
application per week as well as those that were not Effects of staking and pruning on fruit Size
staked and not pruned with two fungicide applications per
week. Rotted fruits were typically firm with greasy spots Plants that were staked and pruned with no fungicides
that eventually became leathery and chocolate brown in applied produced bigger sized fruits similar to those of
color. These spots later increased to the point of plants that were not staked and pruned compared to
encompassing the entire fruit. those that were staked and not pruned with one round of
Staking raises the fruits from the ground and hence fungicides and those not staked and not pruned with two
ensures that they do not come into contact with soil borne rounds of fungicides.
pathogens which are responsible for a number of Pruning reduces over growth of leaves and stems
diseases in tomato fruits including late blight. This gives thereby exposing the plant to sunlight. The plants then
rise to clean and unblemished fruits. Pruning reduced the direct all energy to existing branches and blossoms,
risk of spreading diseases from leaves and stems to fruits producing larger fruits.
as reported by Kavoi (2016). Fungicides help protect the This confirmed the findings of McEwen, (1998), who
fruits from late blight caused by Phytophthora infestans. reported that pruning of tomatoes, gave bigger fruits than
not pruned. The differences that existed among
Effects of staking, pruning and different fungicide treatments may be attributed to the effect of pruning and
frequencies on late blight severity staking.

Plants that were staked and pruned but no fungicide Effects of staking and pruning on fruit yield
applied and those that were staked and not pruned with
fungicide applied once per week had significantly lower Fruit yield was significantly higher in plants that were
values of disease severity compared to those that were staked and not pruned with one round of fungicide per
Tabi et al., 7

week and those that were not staked and not pruned with Haldar K, Kamoun S, Hiller NL, Bhattacherje S, van Ooij C. (2006).
Common infection strategies of pathogenic eukaryotes. Nature
two fungicide applications per week compared to those Reviews Microbiology 4, 992-931.
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well as those that were not staked and pruned with one of Farmers. 5:112-114. 1961
fungicide application per week. Plants that were not J.G. Quinn, An evaluation of methods of mulching and staking tomatoes
grown during the rains at Samaru, Nig. Horti. Res. J. 13:97-
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resulted in high yield of marketable tomatoes in pruned Morogoro, Tanzania. J. Animal and Plant Sci. 6 (3): 663-676.
Mizubuti ESG, Fry WE. (2006). Potato late blight. In: Cooke BM, Jones
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