Professional Documents
Culture Documents
蛋白質純化&定量
蛋白質純化&定量
吸光值
0(PBS) 0.316 0.312 0.314 0 0.314 BSA 樣品
1.2
0.2mg/mL 0.508 0.54 0.524 0.2 0.524
f(x) = 0.780214285714286 x + 0.3668
0.4mg/mL 0.71 0.716 0.713 0.4 0.713 1 R² = 0.953526509216082
0.6mg/mL 0.879 0.874 0.8765 0.6 0.8765
0.8mg/mL 1.036 1.094 1.065 0.8 1.065 0.8
595nm 吸光值
1.0mg/mL 0.985 1.113 1.049 1 1.049 0.713
6N NaOH 0.586 0.619 0.6025 0.6
0.524
6N HCl 0.24 0.227 0.2335
0.4
Triton X-1 1.353 1.578 1.4655 0.314
SDS 0.619 0.654 0.6365 0.2
Unknown s 0.853 0.857 0.855
0
0 0.2 0.4
BSA 標準品
樣本管數吸光值
1 1.171 親和層析圖譜
2 1.555 1.8
1.555 1.588
3 1.375 1.6
1.375
4 0.623 1.4
1.171
5 0.511 1.2
595nm 吸光值
6 0.446 1
7 0.395
0.8 0.653
8 0.379 0.623
0.6 0.511 0.473
9 0.653 0.395 0.35 0.34 0.344
0.4 0.32
10 1.588 0.446 0.379 0.36
0.2 0.322 0.327 0.306
11 0.473
12 0.35 0
0 2 4 6 8 10 12 14 16 18 20
13 0.36
14 0.34 樣本管數
15 0.322
16 0.344
17 0.327
18 0.32
19 0.306
BSA 樣品檢量線
2
f(x) = 0.780214285714286 x + 0.366809523809524 1.065 1.049
1 R² = 0.953526509216082
0.8765
8
0.713
6
0.524
4
0.314
2
0
0 0.2 0.4 0.6 0.8 1 1.2
BSA 標準品濃度 (mg/mL)
34 0.344 0.32
0.322 0.327 0.306
4 16 18 20