Roll Number:

Thapar Institute of Engineering & Technology, Patiala

Department of Biotechnology
MSc. (Second Year-III semester) Course Code: PBT306
Date 5/12/2019 Course Name: Animal & Stem Cell Technology
Time: 3 Hours, M. Marks: 100 Name Of Faculty: Siddharth Sharma, Ph.D.

01(a) How would you characterize the hematopoietic stem cells using marker and functional
analysis (6+4)
01(b) What is therapeutic cloning? Describe both the Roslin and Honolulu technique for animal
cloning (2+4+4)

Q2(a) How can a Transgenic animal be created using the "PRONUCLEI" method. Explain
disadvantage of this method as compared to the ESc method. (5)
02(b) How can transgenic animals be used as "Scientific Models" and as "Xenotransplanters"
(6+4)
02(c) What are the different types of factors influencing the outcome of HSCT (5)

Q3 (a) Briefly explain the importance of doing characterization of animal cells. How can you use
cell surface antigens and intermediate filament proteins for characterization (4+6)
Q3 (b) Explain the LIF-pathways involved in the maintaince of Differentiation and Self renewal
of ESC (10)

Q4(a) What is the "Neoplastic" and "Genetic" properties associated with cells which have
undergone transformation. (4+4)
04(b) Explain what is antisense oligonucleotides technology and how does it work? (6)
Q4(c) A recent publication has shown that the fat stem cells (FSC) can act as bone stem cells to
repair cavities in the skull, when transplanted into immuno-compromised mice. The FSC express
the STRO-1+ cell surface protein. Briefly explain how you can use this information to purify the
FSC from a mixed population that contains multiple cell types. (6)

Q5(a) What are Mesenchymal stem cells. Explain its appealing characteristic properties and the
process of isolation of these cells? (1+3+3)
Q 5(b) What are the properties of aptamers. Explain the application of aptamers in pathway
elucidation and in AIDS (4+4)

Q5 (c) Explain the following: (1 marks each)

(a) Teratoma
(b) Oct4
(c) CD34
(d) T-Banding
(e) STAT