O/C 2-

Name & Roll No:

Thapar Institute of Engineering & Technology, Patiala
Department of Biotechnology
PBT306: Animal & Stem Cell Technology
M.Sc-Biotech Wird Semester) MST
25th September 2019
Time: 02 Hours; MM: 25 Name of faculty: Siddharth Sharma, Ph.D

Section A

1. Describe the application of tissue/cell culture in the fields of Toxicology and Intracellular Flux
(1.5+1.5)

2. What are the advantages and disadvantages of Sub-culture or passage (3)

3. Explain the principle of PI/FDA cell viability assay (3)

4. Give a schematic diagram of the both mechanical and enzymatic disaggregation paths for obtaining
a cell line (2)

5. What are the disadvantages of using serum free media in cell culture (2)

6. Explain the following (0.5 marks each)

(a) Transferrin
(b) KGF
(c) Cryopreservation
(d) Fischer's Media
Section B

1. Describe the correlation between viscosity, surface tension and foaming as a physiochemical

parameter in cell culture? (4)

2. Explain with examples how does non-physiological inducer contribute in the process of differentiation

(3)

3. The total number of cells in a culture is counted using the trypan blue exclusion assay and is found to

be 2.7 x 106 cells/ml. The culture is diluted 1:27 and then •100µI seeded per well into a 96 well plate.

What is the final cell density per well?? (3)

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