BIRLA INSTITUTE OF TECHNOLOGY AND SCIENCE, PILANI- HYDERABAD

CAMPUS
First Semester 2016-2017
Course No.: PHA F215 Course Title: Mol. Bio
Duration: 60 mins. Test I Max. Marks: 30
Closed Book Date: 12/09/2016

Answer all the following questions. Write specific and precise answers.

1. List the three main applications of molecular biology? (3.0)

2. Name the bacteria used in frequently in molecular biology experiments. (4.0)

Give three reasons why it is used frequently?

3. Explain diagrammatically cell fractionation technique? (2.0)

4. Name the two chemical reactions involved in formation and degradation (3.0)

of macromolecules. Give one suitable example for each reaction?

5. What are the 3 stages of cellular respiration? Explain how ATPs are (3.0)
generated in each stage of cellular respiration?

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6. Explain P53 mediated regulation of apoptosis? (3.0)

7. Name the checkpoints involved in cell cycle regulation. Explain how (4.0)

do they control cell cycle.

8. Explain diagrammatically Avery–MacLeod–McCarty experiment? (5.0)

9. List 5 differences between DNA and RNA? (3.0)

 BIRLA INSTITUTE OF TECHNOLOGY AND SCIENCE, PILANI- HYDERABAD
CAMPUS
First Semester 2016-2017
Course No.: PHA F215 Course Title: Mol. Bio
Duration: 60 mins. Test II Max. Marks: 30
Closed Book Date: 21/10/2016

Answer all the following questions. Write specific and precise answers.

1. What are the materials involved in transcription? Explain the function of each? (3.0)

2. Explain the role of promoter region of DNA for transcription? (3.0)

3. Explain the mechanism of RNA processing in eukaryotes? (3.0)

4. What are the stages of translation? Explain each stage diagrammatically? (5.0)

5. List 4 differences between innate and adaptive immunity? (2.0)

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6. Explain the signal transduction pathway of chemokine receptors? (3.0)

7. Explain the mechanism of action of TNF family of receptors? (3.0)

8. Explain the stages of meiosis II? (5.0)

9. Explain the signal transduction of ligand gated ion channels? (3.0)

 BIRLA INSTITUTE OF TECHNOLOGY AND SCIENCE, PILANI- HYDERABAD
CAMPUS
First Semester 2016-2017
Course No.: PHA F215 Course Title: Mol. Bio
Duration: 3 hrs Comprehensive exam Max. Marks: 50
Closed Book Date: 13/11/2016

Answer all the following questions. Write specific and precise answers.
1. After an exam, you and a couple of friends spend a day in the sun on Beach.   At the
end of the day, one of your friends complains of sunburn.  His back is very red,
swollen, warm and painful to the touch. What must have caused the pain and why?
(3.0)
2. John is bitten by a mosquito and observes a lesion at the right arm.  What causes the
erythema and why?  (3.0)

3. For protein blotting, which membrane PVDF or nitrocellulose is used and why?
(3.0)
4. Labelled antibodies are usually used for which blotting technique? (2.0)
5. Compare and contrast the Northern and Western blot procedures by completing the
following parameters: a) Method to denature sample, b) What type of gel you will
run, c) In what direction your samples will run in gel, d) What probe will you use to
detect protein or DNA? (4.0)

6. Cancer cells often have reduced amounts of cell surface proteins, including class I
MHC antigens. Which cells of the immune system can exploit this property to kill
cancer cells? (2.0)

7. In order to initiate an adaptive immune response, antigenic peptide must be presented to

antigen-specific T cells. Which one type of cell presents this antigen to T cells?  (2.0)

8. What is RIP? What is it used for? (2.0)

9. What is the significance of MHC class I molecules? (3.0)

10. Name 3 antigen presenting cells? (3.0)

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11. From a single molecule of DNA, how many copies of PCR can make? (2.0)

12. Genomic libraries are made from? (1.0)

13. Given a single-stranded DNA molecule dissolved in water, what needs to

be added to allow synthesis of a complementary strand? (3.0)

14. what is a cDNA? what enzyme is essential to making cDNAs? (2.0)

15. In order to enter the cell cycle a cell must be stimulated from outside. What type of
molecule provides this stimulation?? (2.0)

16. The passage of a cell through the stages of the cell cycle is controlled by protein
kinases that phosphorylate many different proteins at appropriate times. What are
these protein kinases called? (2.0)

17. At which cell cycle checkpoint is the cell cycle halted if the cell's DNA is
damaged? (1.0)

18. The chemical union of the basic units of carbohydrates, lipids, or proteins always
produces which byproduct? Give suitable example? (3.0)

19. When a protein is boiled, it loses all levels of organization besides the primary level.
When this happens, the protein is said to be? Give one example of such phenomena?
(3.0)

20. Match the following (4.0)

i) RIP a)Protein
ii) CHIP b)RNA
iii) IP c) DNA
iv) Co-IP d) Carbohydrate