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  • QPCR - RT QPCR NEB Ebook 20180814

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    dinesh kumar
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    The document provides an overview of quantitative PCR (qPCR) and reverse transcription quantitative PCR (RT-qPCR). It discusses dye-based and probe-based qPCR assays, including SYBR Green, Taqman probes, molecular beacons, dual hybridization probes, and scorpion probes. The document also covers NEB Luna qPCR and RT-qPCR reagents, their development and benefits over traditional methods, optimization tips, and troubleshooting guidance.

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    qPCR_RT-qPCR-NEB-eBook-20180814

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    The document provides an overview of quantitative PCR (qPCR) and reverse transcription quantitative PCR (RT-qPCR). It discusses dye-based and probe-based qPCR assays, including SYBR Green, Taqman probes, molecular beacons, dual hybridization probes, and scorpion probes. The document also covers NEB Luna qPCR and RT-qPCR reagents, their development and benefits over traditional methods, optimization tips, and troubleshooting guidance.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    14 views28 pages

    QPCR - RT QPCR NEB Ebook 20180814

    Uploaded by

    dinesh kumar
    The document provides an overview of quantitative PCR (qPCR) and reverse transcription quantitative PCR (RT-qPCR). It discusses dye-based and probe-based qPCR assays, including SYBR Green, Taqman probes, molecular beacons, dual hybridization probes, and scorpion probes. The document also covers NEB Luna qPCR and RT-qPCR reagents, their development and benefits over traditional methods, optimization tips, and troubleshooting guidance.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 28

    The Fundamentals of

    qPCR and
    RT-qPCR
    Contact
    TABLE OF

    CONTENTS
    I. Introduction to qPCR and RT-qPCR


    Ia. Quantitative PCR (qPCR)




     ° °

     ° °

    °
    °

     °




    Ib. Reverse transcription quantitative PCR (RT-
    qPCR)


    ° °

    ° °

    ° 



    II. Dye-based qPCR assays

    IIa. SYBR Green dye



    III. Probe-based qPCR assays


    IIIa. Taqman/hydrolysis probes


    IIIb. Molecular beacon probes


    IIIc. Dual hybridization probes

     

     
    IIId. Scorpion probes


    °
    IV. NEB Luna qPCR /RT-qPCR
    IVa. Development – Dots in boxes
    IVb. Benefits to traditional qPCR/RT-qPCR
    Reagents


    IVc.Optimization tips for Luna qPCR/RT-qPCR


     


     °

    °

     °


     






     °





    IVd.Trouble-shooting Luna qPCR and RT-
    qPCR



     


    V. References 



     




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