1

For
Examiner's
Centre Number Candidate Number Candidate Name Use

NAMIBIA SENIOR SECONDARY CERTIFICATE

BIOLOGY ORDINARY LEVEL 4322/3
PAPER 3 Applied Practical Skills 1 hour 30 minutes
Marks: 60 2008

BOOKLET
Additional materials: rulers
calculators

INSTRUCTIONS AND INFORMATION TO CANDIDATES

• Write your Centre Number, Candidate Number and Name in the spaces at the top of this page.
• Write in dark blue or black pen in the spaces provided on the question paper.
• You may use a soft pencil for any diagrams, graphs or rough working.
• Do not use staples, paper clips, highlighters, glue or correction fluid.
• Do not write in the margin For Examiner's Use.

• Answer all questions.

• The number of marks is given in brackets [ ] at the end of each question or part question.

For Examiner's Use

Section A

Total

This document consists of 12 printed pages.

Republic of Namibia
© MoE/DNEA MINISTRY OF EDUCATION [Turn over
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1 5cm3 starch solution was placed in four test tubes labelled A, B, C and D as shown For
Examiner's
in Fig. 1.1. Use

2cm3 saliva was placed in each of tubes B and C.

A B C D

5 cm3 starch
solution
2 cm3 saliva

Fig. 1.1

The tubes were shaken to mix the contents and left for 15 minutes.
After 15 minutes, three drops of iodine solution were added to each of test
tubes A and B.
3cm3 of Benedict's solution were added to test tubes C and D.
Test tubes C and D were placed in a boiling water bath for 5 minutes.
Some of the results are shown in Table 1.1
Table 1.1

tube reagent result

A iodine solution

B iodine solution yellow brown

C Benedict's solution orange precipitate

D Benedict's solution

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(a) State which substance is tested for with Benedict's solution. For
Examiner's
Use
.............................................................................................................................. [1]

(b) Complete the results for test tubes A and D in table 1.1. [2]

(c) Explain the result of test tube B.

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.............................................................................................................................. [2]

(d) The test tubes were kept at 20°C during the time of the experiment.
Predict how the time taken for the colour change in B and C would change if the
test tubes had been kept at 30°C.
Prediction ..............................................................................................................
Explanation ...........................................................................................................
..............................................................................................................................
..............................................................................................................................
.............................................................................................................................. [3]

(e) Describe how you would carry out a test to compare the vitamin C content of two
fruit juice samples.
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[13]

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For
2 Fig. 2.1 shows a potometer which is used to measure the rate of water uptake by a Examiner's
shoot. Use

transpiring shoot,
drawing up water
from the potometer

reservoir containing
water

air/water meniscus
airtight seal screw clip
capillary tube

water

Fig. 2.1

(a) (i) Explain two precautions that must be taken when setting up this
experiment.
Precaution 1 ...................................................................................................
Explanation ....................................................................................................
.......................................................................................................................
.......................................................................................................................
Precaution 2 ...................................................................................................
Explanation ....................................................................................................
.......................................................................................................................
....................................................................................................................... [4]
(ii) As the water is taken up by the shoot, the meniscus moves towards the
left.
Explain how you would move the meniscus back to its original position.
.......................................................................................................................
....................................................................................................................... [1]

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(b) Four sets of apparatus were placed in each of the following laboratory For
Examiner's
conditions: Use

E in classroom conditions (simulates shaded, calm conditions)

F in front of a heater
G set under a clear plastic bag in which water has been sprinkled
H set in front of a fan

15 minutes were allowed for each shoot to adjust to these conditions.

The distance moved by the meniscus was then recorded at 15 minute intervals.
The experiments were repeated three times.

Table 2.1 shows the results.

Table. 2.1

distance moved by meniscus

conditions average distance/m m
every 15 minutes/mm rate =
time/min
1 2 3 average

E in classroom 2.8 2.8 2.6 2.7 0.18

F in front of heater 5.0 6.2 5.3 5.5 0.37

G in plastic bag 1.9 0.8 0.9

H fan 7.1 6.9 8.2

(i) Complete the table by calculating the missing values. [2]

(ii) Suggest which environmental condition is simulated by the fan.
....................................................................................................................... [1]
(iii) Describe the differences in water taken up by the shoots in conditions B
and C.
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(iv) Explain the differences you have described in (iii).
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[12]
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3 Fig. 3.1 shows an apparatus to demonstrate breathing in humans. For

Examiner's
Use

glass y-tube
stopper

balloon

bell jar

plastic sheet

rubber band

Fig. 3.1
(a) Name the part of the human ventilation system that is represented by each of
the following parts of the apparatus.
(i) glass tube ......................................................................................................
(ii) balloons .........................................................................................................
(iii) bell jar ............................................................................................................
(iv) plastic sheet................................................................................................... [4]

(b) (i) Complete the diagrams in Fig. 3.2, to show what happens to the balloons
when the plastic sheet is pulled down (A) and when the plastic sheet is
pushed up (B). [2]
A B

plastic
sheet

pushed up
pulled down
Fig. 3.2

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(ii) Explain how air moves into the lungs during inhalation. For
Examiner's
Use
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(c) Compare the ways in which this apparatus in Fig. 3.1 differs from the human
ventilation system.
1 ............................................................................................................................
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2 ............................................................................................................................
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[13]

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4 Fig. 4.1 shows a young oat seedling. For

Examiner's
Use
When an oat grain germinates, its shoot is enclosed in a sheath of tissue known as
a coleoptile.

coleoptile

food store

young root

Fig. 4.1

The coleoptiles of germinated oat grains will continue to grow if placed in a sucrose
solution. Different concentrations of auxins can be added to the sucrose solution.

The effect of the auxins on the growth of shoots and roots are shown in Table 4.1.

Table 4.1

auxin concentration/ % growth stimulation % growth stimulation

arbitrary units in shoots (coleoptile) in roots

1 5 2

2 15 20

3 25 5

4 45 0

5 65 0

6 95 0

7 75 0

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(a) (i) Plot a graph to show the results for the shoots in Table 4.1. [5] For
Examiner's
Use

(ii) Describe the results for the effect of auxin on the % growth stimulation of
the shoots.
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....................................................................................................................... [2]
(iii) With reference to Table 4.1, compare the results of the shoot and the root
at an auxin concentration of 6 arbitrary units.
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....................................................................................................................... [2]

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(b) State two effects of auxins on the cells at the tip of a shoot. For
Examiner's
Use
1 ............................................................................................................................
..............................................................................................................................
2 ............................................................................................................................
.............................................................................................................................. [2]
[11]

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5 Fig. 5.1 shows the front view of a human eye. For

Examiner's
Use

X Y
Fig. 5.1

(a) Make a large, labelled drawing of this eye. [5]

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(b) The photograph in Fig. 5.1 shows the eye magnified x1.5. For
Examiner's
Use
Measure the distance between points X and Y on Fig. 5.1. Use this measurement
to calculate the magnification of your drawing, allowing for the x1.5 magnification
of the photograph.
Show your working.

Distance between X and Y on photograph: ..........................................................

Distance between X and Y on your drawing: ........................................................

Magnification of your drawing: .............................................................................. [4]

(c) Fig. 5.2 shows front views of a cat’s eye at 3 p.m. and at 3 a.m.

A B
Fig. 5.2

(i) State which one, A or B, shows the appearance of the cat’s eye at 3 a.m.
.......................................................................................................................
(ii) Explain your answer.
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[11]

[TOTAL: 60]

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