Laboratory Report In Biochemistry

DIGESTION
Activity No. 12

Prepared by:
Dugenia, Aries
E spiga, Rhyza
 What is digestion?
IT IS A PROCESS OF
BREAKING DOWN
LARGE MOLECULES
INTO SMALLER
MOLECULES IN THE
PRESENCE OF
ENZYMES.
RESULTS AND DISCUSSIONS
1. PEPTIC DIGESTION
RESULTS AND DISCUSSIONS
2. PANCREATIN DIGESTION
 QUESTION GUIDE:
1. In which tube is there evidence of hydrolysis of protein in peptic
andpancreatin digestion?
PEPCTIC
Tube 3 shows the evidence of hydrolysis of protein in peptic digestion, this is proven by
the massive decrease in the size of the egg white. The addition of pepsin and HCL breaks
down the protein in which denaturation of the protein.

PANCREATIN
Tube 4 shows the evidence of hydrolysis of protein in pancreatin digestion, which is
associated by the large decrease in the size of the egg white. This states that the enzyme
present in the pancreatin breaks down the protein that js present in the egg white into
peptides.
 QUESTION GUIDE:
1. What is the role of pepsin and pancreatin in protein digestion?
Where can these enzymes be found in the body
ROLE OF PEPSIN
It breaks proteins into more easily
absorbed peptides and amino acids
in the small intestine. Pepsin has an
optimum pH of 1.5-2.

Pepsin is found in the gastric chief

cells of the stomach lining.
 QUESTION GUIDE:
2. What is the role of pepsin and pancreatin in protein digestion?
Where can these enzymes be found in the body

ROLE OF PANCREATIN

Trypsin and chymotrypsin are proteasy found in

pancreatin, these proteases are responsible for
the breaking down of proteins into smaller
peptides and eventually amino acids.

Pancreatin is found in the

pancreas.
 QUESTION GUIDE:
3. What is the effect of HCl in peptic digestion? What is the effect of
Alkali in pancreatin digestion?
EFFECT OF HCL
HCl contributes H+ in thr protein digestion, this H+ activates the pepsinogen which is
inactive into pepsin that is active by lowering the acidity /pH of the enzyme environment
into pH of 1.5, which is the optimum pH of pepsin. During digestion, the pepsin acts as a
complex protein that breaks down into amino acids and peptides that is usually absorbed
by our intestin.

EFFECT OF ALKALI
Alkali activates pancreatin by increasing the pH level into 7.4 - 8.2 which is the optimum
pH of pancreatin. The alkali neutralizes the acidity of the stomach enzyme which enables
the softer activation of the enzyme which is the pancreatin. This activation enables the
pancreatin to break down foods that goes to the intestine and convert into energy.
 Inactive Form Activation Active Form

PEPSIN
PEPSINOGEN Factor PEPSIN
HCl

Function Affected Hydrolysis Site

BREAKS DOWN PROTEINS Polypeptides / CARBOXY TERMINAL
INTO SMALLER Amino Acids SIDE (COO-)
POLYPEPTIDES AND AMINO TYROSINE,
ACIDS SUCH AS TYROSINE, TRYTOPHAN, AND
TRYTOPHAN AND
PHENYLALANINE.
PHENYLALANINE THAT CAN
BE EASILY ABSORBED IN
THE SMALL INTESTINE.
 Inactive Form Activation Active Form

TRYPSIN
TRYPSINOGEN Factor TRYPSIN
ENTEROKINASE

Function Affected Hydrolysis Site

BREAKS DOWN SMALLER Polypeptides / CLEAVES THE C-
POLYPEPTIDES SUCH AS Amino Acids TERMINAL OF LYSINE
LYSINE AND ARGININE LYSINE AND AND ARGININE.
INTO PEPTIDES. ARGININE
 Inactive Form Activation Active Form
CHYMOTRYPSINOGEN
Factor CHYMOTRYPSIN
CHYMOTRYPSIN TRYPSIN

Function Affected Hydrolysis Site

CLEAVES OFF PIECES OF Polypeptides / CLEAVES THE C-
AMINO ACIDS FROM THE Amino Acids TERMINAL SIDE OF
PROTEIN CHAIN. TYROSINE, TYROSINE,
SPECIFICALLY TRYTOPHAN, AND PHENYLALANINE,
PHENYLALANINE, TYROSINE, AND TRYPTOPHAN.
PHENYLALANINE.
AND TRYPTOPHAN BONDS,
STARTING FROM THE C-
TERMINAL OF THE PROTEIN.
 Inactive Form Activation Active Form
CARBOXYPEPTIDASE PROCARBOXYPEPTIDASE
PROELASTASE
Factor CARBOXYPEPTIDASE

ELASTASE
ELASTASE
TRYPSIN

Function Affected Polypeptides Hydrolysis Site

THESE ENZYMES TARGET
THE SAME, THEY ATTACK
THE FIRST PEPTIDE AT THE
C-TERMINAL END OF THE
PROTEIN AND RELEASE
AMINO ACIDS ONE AT A
TIME.
/ Amino Acids CARBOXY TERMINAL
ELASTASE: SIDE (COO-)
ALANINE, LYCINE, AND SERINE
CARBOXYPEPTIDASE:
HYDROPHOBIC AMINO ACIDS,
AND BASIC AMINO ACIDS SUCH
AS ARGININE AND LYSINE.
THANK
YOU!