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  • Grafik Kurva Kinetika Enzim

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    africa ayatullah
    18 views4 pages
    The document contains graphs and equations from experiments analyzing enzyme kinetics using different methods like Lineweaver-Burk, Eadie-Hofstee, and Hanes-Wolf plots. It also includes standard curves for the substrate tyrosine and tables with substrate concentrations, absorbance measurements, calculated tyrosine and enzyme concentrations, and Michaelis-Menten constants.

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    GRAFIK KURVA KINETIKA ENZIM

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    The document contains graphs and equations from experiments analyzing enzyme kinetics using different methods like Lineweaver-Burk, Eadie-Hofstee, and Hanes-Wolf plots. It also includes standard curves for the substrate tyrosine and tables with substrate concentrations, absorbance measurements, calculated tyrosine and enzyme concentrations, and Michaelis-Menten constants.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    18 views4 pages

    Grafik Kurva Kinetika Enzim

    Uploaded by

    africa ayatullah
    The document contains graphs and equations from experiments analyzing enzyme kinetics using different methods like Lineweaver-Burk, Eadie-Hofstee, and Hanes-Wolf plots. It also includes standard curves for the substrate tyrosine and tables with substrate concentrations, absorbance measurements, calculated tyrosine and enzyme concentrations, and Michaelis-Menten constants.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 4

    Grafik Lineweaver – Burk

    Kondisi A
    6

    5 y = 4,0402x + 2,0524
    R² = 0,9639
    4
    1/V

    0
    0 0,1 0,2 0,3 0,4 0,5 0,6 0,7 0,8
    1/[S]

    Kondisi B
    14
    y = 11,057x + 4,8137
    12
    R² = 0,9926
    10

    8
    1/V

    0
    0 0,1 0,2 0,3 0,4 0,5 0,6 0,7 0,8
    1/[S]

    Grafik Eadie – Hofstee

    Kondisi A
    0,5
    0,45
    0,4
    0,35
    0,3
    0,25
    V

    0,2 y = -1,9154x + 0,4845


    0,15 R² = 0,8527
    0,1
    0,05
    0
    0 0,02 0,04 0,06 0,08 0,1 0,12 0,14 0,16
    V/[S]
    Kondisi B
    0,2
    0,18
    0,16
    0,14
    0,12
    0,1
    V

    0,08 y = -2,2251x + 0,205


    R² = 0,9836
    0,06
    0,04
    0,02
    0
    0 0,01 0,02 0,03 0,04 0,05 0,06
    V/[S]

    Grafik Hanes – Wolf

    Kondisi A
    45
    40 y = 2,2235x + 3,2379
    R² = 0,9862
    35
    30
    25
    [SV]

    20
    15
    10
    5
    0
    0 2 4 6 8 10 12 14 16 18
    [S]

    Kondisi B
    100
    90 y = 4,8821x + 10,828
    80 R² = 0,9997
    70
    60
    [S/V]

    50
    40
    30
    20
    10
    0
    0 2 4 6 8 10 12 14 16 18
    [S]
    Kurva Eadie-Hofstee
    0,03
    0,025
    0,02
    y = 0,3457x + 0,0166
    0,015
    V

    R² = 0,7622
    0,01
    0,005
    0
    0 0,005 0,01 0,015 0,02 0,025 0,03 0,035
    V/[S]

    Kurva Eadie-Hofstee
    0,03
    0,025
    0,02
    0,015 y = 0,3457x + 0,0166
    V

    0,01 R² = 0,7622

    0,005
    0
    0 0,005 0,01 0,015 0,02 0,025 0,03 0,035
    V/[S]

    Kurva Standart Tirosin


    1
    y = 0,0095x + 0,0151
    0,8 R² = 0,998
    Absorbansi

    0,6

    0,4

    0,2

    0
    0 20 40 60 80 100
    Konsentrasi Larutan Standart Tirosin (mg/ml)
    Kurva Standart Tirosin
    1
    y = 0,0095x + 0,0151
    0,8
    R² = 0,998
    Absorbansi

    0,6
    0,4
    0,2
    0
    0 20 40 60 80 100
    Konsentrasi Larutan Standart Tirosin (mg/ml)

    Konsentrasi Absorbansi Konsentrasi Aktivitas V/[S]


    Substrat 750 nm a b Tirosin Enzim V
    Kasein [S] Sampel
    0,25 0,1147 0,0095 0,0151 10,48 0,0038 0,0038 0,0152
    0,5 0,3129 0,0095 0,0151 31,34 0,0115 0,0115 0,02308
    0,75 0,5102 0,0095 0,0151 51,11 0,0188 0,0188 0,02506
    1 0,6626 0,0095 0,0151 68,15 0,0251 0,0251 0,0251
    1,25 0,8746 0,0095 0,0151 90,47 0,0333 0,0333 0,02664

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