D3 Service Manual, Rev 1

DREW-D3
SERVICE MANUAL
REF : D3-SM-000 Rev 01
The Americas Rest of the World

Drew Scientific Inc. Drew Scientific Co. Limited
Dallas, TX, USA Barrow-in-Furness, Cumbria, UK
Tel : +1 214 210 4900 Tel: +44 (0)1229 432089
Fax : +1 214 210 4949 Fax: +44 (0)1229 432096
Email: productsusa@drew-scientific.com Email:products@drew-scientific.com
http: // www.drew-scientific.com http: // www.drew-scientific.com
REVISIONS
Revision Nb Date Author Software Comments
01 28/10/06 CM/JR >= V 1.0.2 First Release
CONTACT ADDRESS
AUTHORIZED REPRESENTATIVE
The Americas Rest of World
DREW-Scientific DREW-Scientific Co. Limited
4230 Shilling Way Barrow-in-Furness, Cumbria
Dallas, TX 75237, USA United Kingdom
Tel : +1 214 210 4900 Tel: +44 (0)1229 432-089
Fax : +1 214 210 4949 Fax: +44 (0)1229 432-096
Email: productsusa@drew-scientific.com Email: products@drew-scientific.com
http: // www.drew-scientific.com http: // www.drew-scientific.com
LOCAL REPRESENTATIVE
DREW D3 Copyright© Drew Scientific. All Rights Reserved. Page 1/58

IMPORTANT SAFETY INSTRUCTIONS
RISK OF DANGER ! Indicates a procedure to be strictly followed in order to avoid

any risks for the operator (user or technician) or damages to the instrument, or
quality of results.
Indicates that wearing gloves is mandatory before performing the described

operation due to risk of contact with materials that may be infectious.
NOTE : Indicates important additional information.
BEFORE INSTALLING OR
MAINTAINING THE D3, YOU HAVE
TO READ AND REFER TO THE
USER’S MANUAL.
OPERATORS MUST HAVE RECEIVED

ADEQUATE TRAINING.
BE CAREFUL WITH THE EDGES OF THE FRAMEWORK AFTER

HAVING DISMANTLED THE COVERS.
Page 2/58 Copyright© Drew Scientific. All Rights Reserved. DREW D3

This equipment needs special precautions with regard to EMC, and needs to be installed
and put into service according to the EMC information listed below
Guidance and manufacturer’s declaration – Electromagnetic immunity

The D3 is intended for use in the electromagnetic environment specified below. The customer or the user of
the D3 should assure that it is used in such environment.
Immunity test IEC 60601 test level Compliance Electromagnetic environment - guidance
level
Portable and mobile RF communications equipment should
be used no closer to any part of the D3 including cables,
than the recommended separation distance calculated
from the equation applicable to the frequency of the
transmitter.
Recommended separation distance
Conducted RF 3 Vrms 3 Vrms d = 1,2√P

IEC 61000-4-6 150Khz to 80Mhz
Radiated RF 3 Vrms 3 Vrms d = 1,2√P 80MHz to 800MHz

IEC 61000-4-3 80Mhz to 2,5Ghz
d = 2,3√P 800MHz to 2,5GHz
Where P is the maximum output power rating of the

transmitter in watts (W) according to the transmitter
manufacturer and d is the recommended separation
distance in meters (m).
Field strengths from fixed RF transmitters, as

determined by an electromagnetic site survey,a should be
less than the compliance level in each frequency range.b
Interference may occur in the vicinity of equipment
marked with the following symbol:
NOTE 1 At 80Mhz and 800MHz, the higher frequency range applies.

NOTE 2 Theses guidelines may not apply in all situations. Electromagnetic propagation is affected by absorption and reflection from
structures, objects and people.
a
Field strenghts from fixed transmitters, such as base stations for radio (cellular/cordless) telephones and land mobile radios,
amateur radio, AM an FM radio broadcast and TV broadcast cannot be predicted theoretically with accuracy. To assess the
electromagnetic environment due to fixed RF transmitters, an electromagnetic site survey should considered. If the measured field
strenght in the location in which the D3 is used exceeds the applicable RF compliance level above, the D3 should be observed to verify
normal operation. If abnormal performance is observed, additional measures may be necessary, such as re-orienting or relocating the
D3.
b
Over the frequency range 150KHz to 80MHz, field strengths should be less than 3V/m.

TABLE OF CONTENTS
1. INTRODUCTION 6
2. FLUIDICS 7
2.1. INTRODUCTION ......................................................................................................................................................................... 7
2.2. FLUIDICS SEQUENCES DESCRIPTION :..................................................................................................................................... 7
2.3. FLUIDICS VIEW ......................................................................................................................................................................... 9
2.4. FLUIDICS PARTS DESCRIPTION............................................................................................................................................... 10
2.4.1. Sampling module................................................................................................................................................................. 10
2.4.2. Counting bath module........................................................................................................................................................ 10
2.4.3. Syringes module ................................................................................................................................................................. 10
2.5. FLUIDICS DIAGRAM ..................................................................................................................................................................11
2.5.1. General Diagram..................................................................................................................................................................11
2.5.2. Manifold tubing and valves position .............................................................................................................................. 12
2.6. SIZE & LENGTH TUBING .......................................................................................................................................................... 15
3. ELECTRONICS 16
3.1. INTRODUCTION ....................................................................................................................................................................... 16
3.2. ELECTRONICS PARTS VIEW ..................................................................................................................................................... 16
3.2.1. Main board........................................................................................................................................................................... 16
3.2.2. IHM board........................................................................................................................................................................... 17
3.2.3. Preamplifier board ............................................................................................................................................................ 17
3.3. ELECTRONIC DIAGRAM ............................................................................................................................................................ 18
3.4. WIRING DIAGRAM ................................................................................................................................................................... 19
3.4.1. General view ........................................................................................................................................................................ 19
4. FLAGS 20
4.1. GENERAL FLAGS.......................................................................................................................................................................20
4.2. LEUKOCYTE FLAGS ...................................................................................................................................................................20
4.3. ERYTHROCYTE AND HGB FLAGS ............................................................................................................................................. 21
4.4. PLATELET FLAGS ...................................................................................................................................................................... 21
4.5. FUNCTIONAL FLAGS ................................................................................................................................................................ 21
5. OUTPUT FORMAT 22
5.1. DREW FORMAT .......................................................................................................................................................................22
5.1.1. Short Format......................................................................................................................................................................22
5.1.2. Extended Format...............................................................................................................................................................23
5.1.3. CHECKSUM..........................................................................................................................................................................24
6. PARTS LIST & BLOW UP VIEW 26
6.1. PARTS LIST ..............................................................................................................................................................................26
6.1.1. Two years spare parts kit ...............................................................................................................................................26
6.1.2. Tubing kit.............................................................................................................................................................................26
6.1.3. O-rings kit ...........................................................................................................................................................................27
6.1.4. Other spare parts .............................................................................................................................................................27
6.2. BLOW UP VIEW .........................................................................................................................................................................29
6.2.1. Counting Module .................................................................................................................................................................29
6.2.2. Sampling Module ................................................................................................................................................................30
6.2.3. Syringe Module................................................................................................................................................................... 31
6.2.4. CPU-PREAMPLIFIER Module..........................................................................................................................................34
6.2.5. Front cover Module ...........................................................................................................................................................35
7. SOFTWARE 36
7.1. INTRODUCTION .......................................................................................................................................................................36
7.2. SPECIFIC TECHNICIAN DISPLAY .............................................................................................................................................36
7.2.1. Technician password .........................................................................................................................................................36
7.2.2. Technician display..............................................................................................................................................................36
7.3. SOFTWARE FLOW CHARTS .....................................................................................................................................................39
8. MAINTENANCE 40
8.1. MAINTENANCE TABLE .............................................................................................................................................................40
Page 4/58 Copyright© Drew Scientific. All Rights Reserved. DREW D3

8.2. CONCENTRATED CLEANING ..................................................................................................................................................... 41

8.3. PREPERATION FOR SHIPPING OR STORAGE...................................................................... ERROR! BOOKMARK NOT DEFINED.
9. REPAIRING 43
9.1. EMERGENCY STOP ....................................................................................................................................................................43
9.2. SAMPLING MODULE.................................................................................................................................................................43
9.2.1. Needle replacement ..........................................................................................................................................................43
9.2.2. Complete rocker module replacement ..........................................................................................................................43
9.3. COUNTING BATHS MODULE ...................................................................................................................................................45
9.3.1. Baths dismantling...............................................................................................................................................................45
9.3.2. Baths o-ring replacement ................................................................................................................................................45
9.3.3. Aperture block replacement...........................................................................................................................................45
9.3.4. Complete baths module replacement............................................................................................................................45
9.4. SYRINGE MODULE ...................................................................................................................................................................46
9.4.1. Piston greasing....................................................................................................................................................................46
9.4.2. O-ring or piston replacement .........................................................................................................................................46
9.4.3. Complete syringe module replacement .........................................................................................................................47
9.5. BOARDS REPLACEMENT ............................................................................................................................................................48
9.5.1. IHM board replacement ..................................................................................................................................................48
9.5.2. Main board replacement ..................................................................................................................................................50
9.5.3. Main board...........................................................................................................................................................................52
9.5.4. Preamplifier board replacement ....................................................................................................................................54
10. TROUBLESHOOTING 55
10.1. ANALYTICAL PROBLEMS...........................................................................................................................................................55
10.2. OTHER PROBLEMS ....................................................................................................................................................................55
10.3. TROUBLESHOOTING MESSAGE ................................................................................................................................................56

1. INTRODUCTION REF : D3-SM-000 Rev 01
1. INTRODUCTION
BEFORE INSTALLING OR
MAINTAINING A D3, YOU HAVE TO
READ AND REFER TO THE USER’S
MANUAL
The following chapters describe the different technical parts of the D3

- Fluidic
- Electronics
- Technical specification
- Output format
- Spare parts list and blowup view
- Maintenance procedure
Page 6/58 Copyright© Drew Scientific Inc. All Rights Reserved. DREW D3
REF : D3-SM-000 Rev 01 2. FLUIDICS
2. FLUIDICS
2.1. INTRODUCTION
The D3 is a fully automated analyzer performing hematological analysis of whole blood.
The human blood venous sample should be collected in an EDTA K3 (Ethylene Diamine Tetracetic Acid, tri
potassic) tube in sufficient quantity. The 3 Part Differential (Lymphocyte, Mid Cells, Granulocytes) results
are available for six hours after the blood draw. The use of EDTA K2 is not recommended. The sample must
be correctly homogenized before analysis.
The volume of blood must be in correct proportion to the quantity of anticoagulant otherwise
the dilution will affect the result.
Two dilution rates are performed :

~1/237 to 1/257 for WBC/HB (dependant on the lyse volume)
~1/15000 for RBC/PLT
2.2. FLUIDICS SEQUENCES DESCRIPTION :

SAMPLING SEQUENCE :
1- Syringes down : (no valve)
~9,8 µl of blood sample is aspirated inside the sampling needle
Diluent prime
Vacuum prime
2- Needle up and syringe up (valve 3+4)
Needle exterior cleaned with diluent and waste vacuum
3-Rocker moves above the WBC bath
Syringes up to drain the waste (valve 7)
4- Needle down to the rinsing position
5- Syringe down
WBC bath drain (valve 1)
6- Syringe up
Second exterior needle cleaning (valve 4)
Waste syringe drain (valve 7)
7- Syringe down
8- Syringe up
WBC DILUTION :
9- Needle down to the dilution position
Syringes down to prime the diluent (valve 8)
10- Syringe up for the WBC dilution (~2 ml)
Diluent dispensed from outside of the needle (valve 4)
Diluent dispensed from inside the needle (valve 4+5)
Bubble to mix the dilution (valve 1)
RBC DILUTION AND LYSING :

11- Syringe down (no valve)
Aspirating sample of the first dilution (~20µl)
DREW D3 Copyright© Drew Scientific Inc. All Rights Reserved. Page 7/58
2. FLUIDICS REF : D3-SM-000 Rev 01
Diluent prime
Vacuum prime
12- Needle up and syringe up
Rocker moves above the RBC bath
Needle exterior cleaned with diluent and waste vacuum (valve 3+4)
13- Needle down to the rinsing position
14 Syringe up
Second outside needle cleaning (valve 4+8)
Lyse in the WBC bath (0,3 to 0,5ml) (valve 9) (Species dependant)
Bubble to mix the dilution (valve 1)
15 Syringe down
RBC bath drain (valve 2)
16- Needle down to the dilution position
17- Syringe up for the RBC dilution (~1,5ml)
Diluent dispensed from outside of the needle (valve 4+8)
Diluent dispensed from inside the needle (valve 5)
Bubble to mix the dilution and diluent to rinse the counting head (valve 6+10+2)
MEASUREMENTS :
18- Syringe down to establish the counting vacuum (valve 10)
19- First measurement sequences (valve 10)
20- Syringe up
Diluent back pressure (valve 4+6)
21- Syringe down to establish the counting vacuum (valve 10)
22- Second measurement sequences (valve 10)
DRAIN AND RINSE BATH :

22- Syringe down
Cleaning the apertures (valve 11)
RBC bath drain (valve 2)
23- Syringe up
RBC bath rinse diluent (valve 4)
24- Needle up and syringes up for back flush (valve 4+6)
25- Rocker move above the WBC bath
26- Syringe up
WBC bath rinse diluent (valve 4)
NEEDLE BACK AND WASTE DRAIN :

27- Syringe down
Dry the exterior of the needle (valve 3)
28- Rocker moves into the sampling position
26- Syringe up
30- Needle moves down into sampling position
The D3 is ready to perform a new analysis.
2.3. FLUIDICS VIEW
All the fluidics parts are on the right side of the instrument and consist of only three modules :
- Sampling module :
o Rocker (patent pending) : Manages the Sample Needle positioning.
- Syringe module (patent pending) consists of one block :
o Reagent syringes (Diluent, lyse), sampling and air syringes.
o Liquid valve manifold assembly and tubing.
- Counting module :
o WBC and RBC counting chambers and hemoglobin measurement.
o Liquid valve manifold assembly and tubing.
Sampling module
Syringe Module
Counting module

2.4. FLUIDICS PARTS DESCRIPTION

The hydraulic section of the D3 is very simple and made up of only three modules :
- Sampling module.
- Counting module.
- Syringes module.
The modules are connected together by semi rigid tubing.
2.4.1. Sampling module

This module (patented) is responsible for aspirating the sample and performing the WBC and RBC/Plt
dilutions.
It is assembled with a rocker assembly which moves the sample needle from the Aspiration position into the
diluting position for performing the WBC/Hgb and RBC/PLT dilutions.
A very reliable system of rack-gear moves the rocker.
The cleaning system of the sampling needle can be removed without tools (see section 9 in the user manual).
The needle o-ring can be removed without tools (see section 9 in the user manual).
2.4.2. Counting bath module

This module is responsible for counting the WBC and RBC/Plt and measuring the HGB.
It is made with a manifold which contains the reagent valves and the counting bath block including the
apertures.
The counting bath block assembly and apertures can be removed without tools (see section 9 in the user
manual).
2.4.3. Syringes module

This module (patent pending) performs the following tasks :
Aspirate the sample.
Dispense the reagents.
Drain the baths.
Establish the counting vacuum.
Push the waste to the waste container.
The manifold consists of the fluid valves and the five syringes, syringe block including:
Sample syringe
Lyse syringe
Two waste and vacuum/pressure syringes
Diluent syringe.
One motor drives the five syringes.
The diluent intake and the waste output are also included in this manifold.
2.5. FLUIDICS DIAGRAM

2.5.1. General Diagram

2.5.2. Manifold tubing and valves position

Each figure corresponds to a tubing or a valve number (see the Fluidic diagram section 2.5.1)
Counting manifold tubing position
Counting manifold valve positions
Syringe manifold tubing position
Syringe body tubing position

Syringe manifold valves position
2.6. SIZE & LENGTH TUBING
**To ensure quality of results, it is mandatory to adhere to the size and the length of the tubing.**
Tygon Tygon Tygon Tygon Tygon Tygon

tubing tubing tubing tubing tubing tubing
0,8x2,4mm 1,3x3mm 1,6x3,2mm 2x4mm 3x6mm 2.8x5mm
Bulk Tubing Part
Number Designation
FRU-9318-082 Tubing 1 - Lyse 1000mm
FRU-9318-066 Tubing 2 250mm
FRU-9318-083 Tubing 3 - Diluent 150mm 810mm
FRU-9318-064 Tubing 10 370mm 10mm
FRU-9318-066 Tubing 13 250mm
FRU-9318-066 Tubing 14 250mm
FRU-9318-084 Tubing 15 - Cleaner 1000mm
FRU-9318-066 Tubing 16 130mm
FRU-9318-066 Tubing 20 130mm
FRU-9318-066 Tubing 21 600mm
FRU-9318-065 Tubing 22 200mm
FRU-9318-058 Tubing 24 - Waste 1500mm

3. ELECTRONICS REF : D3-SM-000 Rev 01
3. ELECTRONICS
3.1. INTRODUCTION
Three boards compose the D3:
- the Main board
- the Preamplifier board
- and the IHM board
- Ensure correct protection is used when replacing the main board or preamplifier board, as
these are sensitive to electro static discharge (ESD).
- Before operating check the earth connection and use the antistatic bag supply with the
board. Please contact your authorized Drew distributor for more information.
- Be careful with the edges of the frame after having removed the covers.
3.2. ELECTRONICS PARTS VIEW

3.2.1. Main board
The Main board is located between the hydraulic compartment and the reagent tray.
The board, driven by a 32-bit processor, controls the following modules:
- Sample needle, rocker, syringe block motors.
- Display and keyboard.
- External communications (RS232, Ethernet, …).
- Printer.
- Measurement (Counting, hemoglobin measurement).
- Data processing.
- External barcode reader.
REF : D3-SM-000 Rev 01 3. ELECTRONICS
3.2.2. IHM board
3.2.3. Preamplifier board
3.2.4 Power supply Module (D3 is supplied with an external power supply module)
- The D3 has been certified with the power supply provided. The use of another external
power supply box may affect performance and will invalidate the Warranty. Please contact
Drew Scientific or authorized Drew Distributor.

3. ELECTRONICS REF : D3-SM-000 Rev 01
3.3. ELECTRONIC BLOCK DIAGRAM
Page 18/58 Copyright© Drew Scientific All Rights Reserved. DREW D3

REF : D3-SM-000 Rev 01 3. ELECTRONICS
3.4. WIRING DIAGRAM

3.4.1. General view

4. FLAGS REF : D3-SM-000 Rev 01
4. FLAGS
D3 manages different Flags and Alarms. These Flags allow the user to be alerted if there is a situation which
can affect the quality of results. All of these Flags appear to the right of the result.
In presence of one or more Flags, it is recommended to check the result by manual methods
such as a blood smear.
NOTE : Most of these Flags can be modified by the user (see section 3 of the User’s Manual).
4.1. GENERAL FLAGS

The following alarms are common for all parameters.
* : Counting or measure rejection . Can appear with WBC, RBC, PLT and HGB.
XXXD : Higher than linearity limits but lower than the reportable limits. WBC, RBC, PLT, HCT, HGB.
+++D : Higher than the reportable limits. WBC, RBC, PLT, HCT, HGB.
---- : Rejected value.
+ : results higher than normal value.
- : results lower than normal value.
H : results higher than action value.
B : results lower than action value.
4.2. LEUKOCYTE FLAGS
CL1 CL1-2
CL2-2 CL2 CL2-3 CL4 CL5
FLAG CHANNELS MEANING % VALUE # VALUE

L1 CL1 to CL1-2 Possible Platelet aggregate Percentage of cells in the Number of cells in the
or erythroblast zone in reference to the zone.
number of lymphocytes.
L2 CL2 to CL2-2 Possible presence of Percentage of cells in the Number of cells in the
Myelocytes, lymphoblast or zone in reference to the total zone.
basophiles number of leukocytes.
L3 CL2 to CL2-3 Possible presence of Percentage of cells in the Number of cells in the
eosinophils, myelocytes zone in reference to the total zone.
number of leukocytes.
L4 CL4 Granulocyte volume too low Not Available Channel value
L5 CL5 to the end Possible presence of large Percentage of cells in the Number of cells in the
size cells zone in reference to the zone.
number of granulocytes.
REF : D3-SM-000 Rev 01 4. FLAGS
4.3. ERYTHROCYTE AND HGB FLAGS
CR1 CR2

R1 0 to CR1 Abnormal quantity of Percentage of cells in the Number of cells in the
microcytes at the left side of zone in reference to the zone.
the histogram number of erythrocytes.
R2 CR2 to the Abnormal quantity of Percentage of cells in the Number of cells in the
end macrocytes at the right side zone in reference to the zone.
of the histogram number of erythrocytes.
* Appear Abnormal measurement of the Not Available Not Available
with HGB HGB blank.
result.
4.4. PLATELET FLAGS
CP3-2
P2 CP1 CP2 P CP3

FP1 0 to CP1 Presence of abnormal Percentage of cells in the Number of cells in the
quantity of debris or small zone in reference to the zone.
cells number of platelets until P.
FP2 P to CP2 Presence of schizocytes Percentage of cells in the Number of cells in the
zone in reference to the zone.
number of platelets until P.
FP3 CP3 to CP3-2 Presence of microcytes Percentage of cells in the Number of cells in the
zone in reference to the zone.
number of platelets until P.
4.5. FUNCTIONAL ALARMS

INS_T = Means that the working temperature is lower than 17°C or higher than 33°C and the result could
be affected.
INS_P = Means that the counting vacuum is out of range. If the flag appears at each cycle, please contact
your local representative.
W-CL or R-CL = Means that the difference between the Count 1 raw data – Count 2 raw data exceeds
expectations which indicate a disruption in the sample flow.

5. OUTPUT FORMAT REF : D3-SM-000 Rev 01
5. OUTPUT FORMAT
The choice of the setting (format, speed …) is explained in page N° 24 of the User’s Manual.
5.1. DREW FORMAT
5.1.1. Short Format
Byte Format Description Notes
location Size
1 B1 STX(02h)
2-7 A6 Sequence Number
8-22 A15 Id Number Sample ID, sample N° *Note1
23-37 A15 Patient Id PID *Note1
38-67 A30 Last Name ID, patient name *Note1
68 - 77 A10 Date(DD/MM/YYYY)
78- 88 A11 Time(HH:MM:SS AM/PM)
89 A20 Reserved for future parameter expansion
109 A1 1=WBC Clog 1 if alarm else space
110 A1 1=RBC Clog 1 if alarm else space
111 A1 1=Vacuum Failure 1 if errors else space
112 A1 1=Hgb Sensor Not available
113 A1 1=Uncontrolled 1 if alarm else space
114 A1 1=Uncalibrated 1 if alarm else space
115 - 124 A10 Alarmes : L1L2L3L4L5R1R2P1P2P3 1 if alarm else space
125 - 146 A22 Reserved for future parameter expansion
147 – 151 A5 WBC *Note2
152 – 156 A5 LYM N *Note2
157 – 161 A5 LYM P *Note2
162 – 166 A5 MID N *Note2
167 – 171 A5 MID P *Note2
172 – 176 A5 GRA N *Note2
177 – 181 A5 GRA P *Note2
182 – 186 A5 RBC *Note2
187 – 191 A5 HGB *Note2
192 – 196 A5 HCT *Note2
197 – 201 A5 MCV *Note2
202 – 206 A5 MCH *Note2
207 – 211 A5 MCHC *Note2
212 – 216 A5 RDW *Note2
217 – 221 A5 PLT *Note2
222 – 226 A5 MPV *Note2
227 – 231 A5 PCT *Note2
232 – 236 A5 PDW *Note2
237 A1 Sample Type: 1=Patient 2=Background, Always 1 : Patient results only are
3=Calibration 4=Low Control sent
5=Normal Control 6=High Control
238 A1 Sample Mode : 1=Direct, 2=Prediluted
239 - 248 A10 Blood Type name *Note1
249 – 250 A2 Very Simple Checksum *Note3
251 B1 ETX(03h)
A: Alphanumeric ASCII Format

B: Binary Format

REF : D3-SM-000 Rev 01 5. OUTPUT FORMAT
Note 1 : alphanumeric field, coded in UTF8, Truncated if too long.

Note 2 : Same numeric value as displayed on screen (same unit and decimal part).
In case of no validity, the Numeric value is replaced by 5 dots (2eh)
In case of overflow, the Numeric value is replaced by 5 ‘Plus’ (2bh)
Note 3 : Checksum is the LSB of the sum from byte2 to 248 include. It is on two hexadecimal ASCII
characters.
5.1.2. Extended Format

In Extended Format, each packet should be acknowledged by the LIS.
D3 automaton LIS
The packet
Waiting for ACK, contents a
adjustable Time out checksum
Send header packet , type T
LIS acknowledgement ACK (06h)
Send result packet , type N
Send WBC histogram packet , type H

Send RBC histogram packet , type H
Send PLT histogram packet , type H
End of transmission EOT (06h)
Communication Protocol
If timeout occurs when waiting for ACK, EOT is sent and the transmission stops.

5. OUTPUT FORMAT REF : D3-SM-000 Rev 01
5.1.2.1. Packets
header packet description : type T
Byte format description notes
location size
1-6 A6 $$TXMB Header pattern
7-8 B2 AA AA = packet type N number, 2 bytes in binary. MSB then LSB.
9 - 10 B2 BB BB = packet type N size, 2 bytes in binary. MSB then LSB.
11 – 12 B2 CC CC = packet type H number, 2 bytes in binary. MSB then LSB.
13 - 14 B2 DD DD = packet type H size, 2 bytes in binary. MSB then LSB.
15 - 16 B2 EE checksum = LSB sum from 1 to 14 includes bytes. 2 bytes in binary.
TOTAL size = 16 bytes
result packet description : type N

Byte size description notes
location
1-251 251 Light Format packet Matches exactly with light format described previously
histogram packet description : type H, size = 261 bytes

Byte location size description notes
1 1 STX (02h) Binary
2 1 type ASCII, ‘R’ = RBC histogram, ‘W’ = WBC histogram
‘P’ = PLT histogram
3 - 258 256 128 channels Binary, 2 bytes for each channel, MSB then LSB
259 - 260 2 checksum Binary, LSB sum of bytes from 1 to 258 included
261 1 ETX (03h)
TOTAL = Header + result + 3 * histograms + EOT = 16 + 251 + 261 +261 + 261 + 1 = 1051 bytes
5.1.3. CHECKSUM
For frames with few numbers of lines, the block is finished with an end line containing a checksum. This
checksum is calculated from the beginning of the headline to the end of the line finishing the block.
The algorithm of the calculation for the checksum is :
CRC = 0xFFFF
For each byte
Work on high order byte:
Index = byte XOR CRC
Index = Index AND 000F
CRC = Table(Index) XOR (CRC divided by 16)
Work on low order byte:
Index = byte divided by 16
Index = Index XOR CRC
Index = Index AND 000F
CRC = Table(Index) XOR (CRC divided by 16)

REF : D3-SM-000 Rev 01 5. OUTPUT FORMAT
Example in C language:
/*Hash table :*/
static const unsigned short ausCrcTab1[] =
{
0x0000, 0xCC01,0xD801,0x1400,0xF001,0x3C00,0x2800,0xE401,
0xA001,0x6C00,0x7800,0xB401,0x5000,0x9C01,0x8801,0x4400,
};
/* CRC computing */
unsigned short calc_crc(unsigned char *pucData, long lSize)
{
unsigned short usAcc1 = 0xFFFF;
while ( lSize > 0 )

{
/* gestion par quartet du calcul */
usAcc1 = ausCrcTab1[(*pucData ^ usAcc1) & 15] ^ (usAcc1 >> 4);
usAcc1 = ausCrcTab1[((*pucData >> 4) ^ usAcc1) & 15] ^ (usAcc1 >> 4);
pucData++;
lSize--;
}
return(usAcc1);
}
^ : XOR.
>> : Logical shift right.
& : logical AND.

REF : D3-SM-000 Rev 01 6. PARTS LIST & BLOW UP VIEW
6. PARTS LIST & BLOW UP VIEW

6.1. PARTS LIST
6.1.1. Two years spare parts kit
Part Number : FRU-9318-069

Designation : D3 (2 years) spare parts kit
QTY Part Number Description

2 FRU-9318-034 O-Rings kit
1 FRU-9318-068 D3 Tubing kit
2 FRU-9318-051 Piston Ø 22 – Waste
1 FRU-9318-033 Sampling needle
1 FRU-9318-052 Piston Ø 6.5 - Syringes module - Lyse
1 FRU-9318-049 Piston Ø 16 - Syringes module - Diluent
6.1.2. Tubing kit

Designation : D3 Tubing kit
QTY Designation
1 Tubing 1 - Lyse
1 Tubing 2
1 Tubing 3 - Diluent
1 Tubing 4
1 Tubing 5
1 Tubing 6
1 Tubing 7
1 Tubing 8
1 Tubing 9
1 Tubing 10
1 Tubing 11
1 Tubing 12
1 Tubing 13
1 Tubing 14
1 Tubing 15 - Cleaner
1 Tubing 16
1 Tubing 17
1 Tubing 18
1 Tubing 19
1 Tubing 20
1 Tubing 21
1 Tubing 22
1 Tubing 24 - Waste
1 Tubing 61

6.1.3. O-rings kit

Designation : D3 O-Rings kit
QTY Part Number Designation

1 FRU-9318-038 O-ring Ø15.54x2.62 Silicone 60SH – Diluent Piston
2 FRU-9318-041 O-ring Ø21.89x2.62 Silicone 60SH – Waste Piston
1 FRU-9318-045 O-ring Ø6.3x2.4 Silicone 60SH – Lyse Piston
2 FRU-9318-037 O-ring Ø13.1x1.6 Fluocarbon 80SH – Counting Chambers
3 FRU-9318-036 O-ring Ø1.4x1.25 Fluocarbon 80SH – Sampling Needle
2 FRU-9318-043 O-ring Ø5x1 Fluocarbon 80SH - Apertures
6.1.4. Other spare parts
D3 Spare Parts Rev 1
Designation Part Number Qty/Pkg Unit

D3-Cleaner Straw FRU-9318-084 1 UNIT
D3-Lyse Straw FRU-9318-082 1 UNIT
Flexible Flat Cable FRU-9318-003 1 UNIT
70W switching adapter FRU-9318-001 1 UNIT
Bottle cap filter FRU-9318-062 10 UNIT
Counting Chambers FRU-9318-013 1 UNIT
Counting Manifold FRU-9318-014 1 UNIT
Counting Valves Cable FRU-9318-004 1 UNIT
Electrovalve 2/2 FRU-9318-007 1 UNIT
Electrovalve 3/2 FRU-9318-008 1 UNIT
Electrovalve 3/2 - 1.6mm FRU-9318-009 1 UNIT
Fluidic Door Switch FRU-9318-011 1 UNIT
D3-Front cover FRU-9318-002 1 UNIT
Lubriplate DS-ES FRU-9000-033 1 UNIT
Lyse Spring FRU-9318-047 1 UNIT
D3 (2 years) spare part kit FRU-9318-069 1 UNIT
D3 CPU Board FRU-9318-019 1 UNIT
D3 IHM Board FRU-9318-021 1 UNIT
O-Rings kit FRU-9318-034 1 UNIT
Preamplifier Board FRU-9318-018 1 UNIT
D3 Tubing kit FRU-9318-068 1 UNIT
Needle Belt FRU-9318-025 1 UNIT
Needle carriage FRU-9318-026 1 UNIT
Needle Motor FRU-9318-027 1 UNIT
Needle Optical Sensor FRU-9318-028 1 UNIT
O-ring Ø1.4x1.25 Fluocarbon 80SH, Sampling Needle FRU-9318-036 10 UNIT
O-ring Ø13.1x1.6 Fluocarbon 80SH, Counting Chambers FRU-9318-037 10 UNIT
O-ring Ø15.54x2.62 Silicone 60SH, Diluent Piston FRU-9318-038 10 UNIT
O-ring Ø21.89x2.62 Silicone 60SH, Waste Piston FRU-9318-041 10 UNIT
O-ring Ø5x1 Fluocarbon 80SH, Apertures FRU-9318-043 10 UNIT
O-ring Ø6.3x2.4 Silicone 60SH, Lyse Piston FRU-9318-045 10 UNIT
O-Ring spacer FRU-9318-035 1 UNIT
Piston Carriage FRU-9318-048 1 UNIT
6. PARTS LIST & BLOW UP VIEW REF : D3-SM-000 Rev 01
Designation Part Number Qty/Pkg Unit

Piston Ø 16, Diluent FRU-9318-049 1 UNIT
Piston Ø 22, Waste FRU-9318-051 1 UNIT
Piston Ø 6.5, Lyse FRU-9318-052 1 UNIT
Plastic grease SPG35 FRU-9000-035 1 UNIT
RBC Counting Head FRU-9318-015 1 UNIT
Rinsing head FRU-9318-032 1 UNIT
Rocker Motor FRU-9318-029 1 UNIT
Rocker Optical Sensor FRU-9318-031 1 UNIT
Sampling module FRU-9318-024 1 UNIT
Sampling needle FRU-9318-033 1 UNIT
Syringe Body Seal plate FRU-9318-060 1 UNIT
Screw Kit FRU-9318-081 1 Unit
Short Arm TORX T10 Tool FRU-9000-031 1 UNIT
Short Arm TORX T20 Tool FRU-9000-032 1 UNIT
Start key Switch FRU-9318-01 1 UNIT
Stepper motor cable FRU-9318-006 1 UNIT
Syringe Body FRU-9318-053 1 UNIT
Syringe Motor FRU-9318-054 1 UNIT
Syringe Motor Holder FRU-9318-055 1 UNIT
Syringe Optical Sensor FRU-9318-056 1 UNIT
Syringe Piston Ø 1.6 FRU-9318-057 1 UNIT
Syringe Valves Cable FRU-9318-005 1 UNIT
Temperature Sensor FRU-9318-017 1 UNIT
D3-Tubing 3 - DILUENT FRU-9318-061 1 UNIT
D3-Tubing 24 - WASTE FRU-9318-058 1 UNIT
Tubing Ground Cable FRU-9318-063 1 UNIT
Tygon tubing 0,8x2,4mm FRU-9318-064 1000 mm
Tygon tubing 1,3x3mm FRU-9318-065 1000 mm
Tygon tubing 1,6x3,2mm FRU-9318-066 5000 mm
Tygon tubing 2x4mm FRU-9318-067 5000 mm
WBC Counting Head FRU-9318-016 1 UNIT

6.2. BLOW UP VIEW

6.2.1. Counting Module
Rep Part Number Designation

1 FRU-9318-013 Counting chambers
2 FRU-9318-014 Counting manifold
3 FRU-9318-016 WBC Counting Head
4 FRU-9318-015 RBC Counting Head
5 FRU-9318-043 O-ring Ø5x1 Silicone 80SH
6 FRU-9318-081 Screw CZX M3 x 6
7 FRU-9318-037 O-ring Ø13.1x1.6 Fluocarbon 80SH
6.2.2. Sampling Module

1 FRU-9318-024 Sampling module
2 FRU-9318-029 Rocker Motor
3 FRU-9318-027 Needle Motor
4 FRU-9318-032 Rinsing head
5 FRU-9318-033 Sampling Needle
6 FRU-9318-036 O-ring Ø1.4x1.25 Fluocarbon 80SH
7 FRU-9318-026 Needle carriage
8 FRU-9318-025 Belt pitch 2.032 (l=4.763-L=243.84)
9 FRU-9318-028 Needle Optical Sensor
10 FRU-9318-081 Screw K 30 x 8
11 FRU-9318-081 Screw RLX M3 x 6
13 FRU-9318-031 Rocker Optical Sensor
14 FRU-9318-081 Lock washer Ø3 x 1

6.2.3. Syringe Module

1 FRU-9318-053 Syringes body
2 FRU-9318-060 Syringe Body Seal plate
3 FRU-9318-049 Piston Ø 16, Diluent
4 FRU-9318-051 Piston Ø 22, Waste
5 FRU-9318-052 Piston Ø 6.5, Lyse
6 FRU-9318-057 Syringe Piston Ø 1.6, Sampling
7 FRU-9318-038 O-ring Ø15.54x2.62 Silicone 60SH, Diluent
8 FRU-9318-041 O-ring Ø21.89x2.62 Silicone 60SH, Waste
9 FRU-9318-045 O-ring Ø6.3x2.4 Silicone 60SH, Lyse
10 FRU-9318-036 O-ring Ø1.4x1.25 Fluocarbon 80SH, Sampling
11 FRU-9318-035 O-ring spacer
12 FRU-9318-081 Screw K 30 x 10

1 FRU-9318-054 Syringes Motor
3 FRU-9318-081 Screw K 30 x 10
6 FRU-9318-056 Syringe Optical Sensor
7 FRU-9318-081 Screw K 30 x 8


1 FRU-9318-055 Syringes motor holder
2 FRU-9318-053 Syringes body
3 FRU-9318-081 Plain washer Ø3
5 Purchase Locally Tubing 26, 5mm x 8mm
6.2.4. CPU-PREAMPLIFIER Module

2 FRU-9318-019 D3 CPU board
3 FRU-9318-018 D3 Preamplifier board
6 Purchase Locally Hexagonal spacer FF M4 x 60
8 FRU-9318-081 Screw RLX M4 x 8

6.2.5. Front cover Module

1 FRU-9318-002 Front cover
2 FRU-9318-012 Start key Switch
REF : D3-SM-000 Rev 01 7. SOFTWARE
7. SOFTWARE
7.1. INTRODUCTION
Please refer to the User’s Manuel for an explanation of the software operation.
7.2. SPECIFIC TECHNICIAN DISPLAY

The following section describes the software operation which is only accessible to the technician and super
technician passwords.
7.2.1. Technician password

Contact Drew Scientific for Password List
- It is strictly forbidden to disclose the technician password to the End User

- In such cases, the D3 warranty becomes invalid.
7.2.2. Technician display

The technician display is accessible from MAIN SCREEN, MAINTENANCE then SERVICE.
7.2.2.1. Service
System error log.
For an access to the gain adjustment and HGB blank adjustment
Sets D3 Gain settings and Thresholds.
Not Available at this time.
Generates a Service Intervention Entry in the Maintenance Log.

7.2.2.2. Counting parameters
- The modification of one of these parameters must be done in compliance with Drew
Scientific after receiving notice to do so.
- In the case of modification without authorization, Drew Scientific warranty will
not be valid.
- The access of this display is available with the super technician

password only
- The twenty types of blood can be changed and selected from the
drop-down menu:
- This display enables modification of the HGB, WBC, RBC and PLT gain
located in the upper window and the counting pulse parameters in the
lower window.
7.2.2.3. Gain adjustments
- The modification of one of these parameters must be done in compliance with Drew
Scientific and only after specific training.
- In the case of modification without authorization, Drew Scientific warranty
becomes invalid.
- Not available : manufacturing parameters.
- Allows adjustment of the HGB blank level.
- Allows adjustment of the WBC gain with the use

of special latex particles.
- Allows adjustment of the RBC and PLT gain with

the use of special latex particles.
- : ‘Tick’ means that the adjustments are automatically done.
The columns to the right of the gain setting signify the Mean Channel of Sample run and Peak Channel of
Sample run.
Preparing for adjustment:
● Ensure the ; AUTO Tickbox is selected to allow D3 to automatically perform adjustment.

● Latex Preparation. (Use only Latex supplied by Drew-Scientific)
7. SOFTWARE REF : D3-SM-000 Rev 01
RBC/PLT Latex: Add 4 drops of vigorously mixed 3um latex particles and 6 drops of
vigorously mixed 5um latex particles into a small sample tube.
WBC Latex: Add 10 drops of vigorously mixed 7.0um latex particles into a small
sample tube.
● From the Main Menu, go to Maintenance > Service > Prod Adjust.
● Start the <<ADJUST HGB>>> cycle by pressing the soft key. (make sure that the system has been
properly primed and the service door is closed) The system will perform an HGB Blank measurement.
● Vortex the WBC Latex for 10 seconds.

● Present the WBC Latex to the Sample Probe and press the LATEX WBC soft key to start the
Gain Adjustment cycle.
● Vortex the RBC/PLT Latex mixture for 10 seconds.

● Present the RBC/PLT Latex mixture to the Sample Probe and press the LATEX RBC/PLT soft key
to start the Gain Adjustment cycle.
● Verify results meet the criteria below.

● Use the SAVE key and EXIT to validate results.
Gain Limites:
HGB 150 < Gain < 250

870 < Value < 930
WBC 210 < Gain < 242

66 < Value 1 < 72 71 < Value 2 < 73
RBC 210 < Gain < 250

40 < Value 1 < 44 42 < Value 2 < 46
PLT 215 < Gain < 250

51 < Value 1 < 55 54 < Value 2 < 57

7.3. SOFTWARE FLOW CHART
REF : D3-SM-000 Rev 01 8. MAINTENANCE
8. MAINTENANCE
The quality and reliability of the results of the D3 are directly linked to the strict adherence to the
maintenance described hereafter.
To perform the maintenance and repair described in this section, it is mandatory to wear
rubber gloves and wash hands with a disinfectant after completion of work.
8.1. MAINTENANCE TABLE
NOTE : This table is valid for an average number of 50 samples per day. For more, please increase the
maintenance proportionally.
MAINTENANCE DAILY WEEKLY MONTHLY SEMI ANNUAL ANNUALLY

User Tech User Tech User Tech User Tech User Tech
Reagents level X
Start Up X
Automatic cleaning X
Concentrate cleaning X
Shut down X
Cover cleaning X
Piston greasing X X
Needle o-ring replacement X

Syringes o-ring replacement X
Motor screw greasing X
- Reagents level control : see User’s Manual.

- Start up : see User’s Manual.
- Automatic Cleaning : see User’s Manual.
- Concentrated cleaning : see User’s Manual.
- Shut down : see User’s Manual.
- Cover cleaning : see User’s Manual.
- Piston greasing : see User’s Manual.
- Needle o-ring replacement : see section 9 of Operators Manual

- Syringes o-ring replacement : see section 9 of Operators Manual
- Motor screw greasing : This operation is described during training.

REF : D3-SM-000 Rev 01 8. MAINTENANCE
8.2. CONCENTRATED CLEANING

This cleaning may be necessary when the D3 has a permanent alarm for one or more measured parameters.
Prepare a Sodium Hypochlorite solution at 25% in Distilled Water.
- Return to the main menu by pressing .

- Then press .
- Press .
- Press to start the cycle (drain the

counting chamber).
8. MAINTENANCE REF : D3-SM-000 Rev 01
- Wait for the information window.

- Open the door on the right side.
- Put 2 ml of sodium hypochlorite solution in each counting
chamber.
- Press the button in the window located in the middle of the
screen.
- D3 performs a maintenance cycle of the apertures followed by
a stand by mode of 2 minutes.
- After 2 minutes the D3 rinses all of the elements. Operator
should then perform a background test.
-
Wear rubber gloves and wash hands with a
disinfectant after completion of work.
8.3. PREPERATION FOR SHIPPING OR STORAGE

This procedure has to be used when the D3 must be transported for a long distance by car, train or plane.
This procedure consists of cleaning the D3 with sodium hypochloride, then rinsing it with distilled water and
drying it before the transportation.
-
-- The cleaning is accessible from MAIN SCREEN,
, then .
-- Follow the screen prompts to Bleach the baths, flush the
D3 with Distilled Water, and Drain all fluid from the
D3.
*** Ensure all steps are followed to avoid reagents
drying in the lines or fluid spills occurring during
shipments ***

9. REPAIRING REF : D3-SM-000 Rev 01
9. REPAIRING
For all the following operations wear rubber gloves and wash hands with a disinfectant after
completion of work.
Be careful with the edges of the frame after removing the covers.
9.1. EMERGENCY STOP

In case of mechanical or hydraulic problem, immediately press , the D3 will make an emergency stop.
- After having identified the problem, it is necessary to perform a Reset cycle.
- This option is accessible from the MAIN MENU,

, :
then
- The Reset Cycle will initialise all motors and fluidics into the Stand-by position.
9.2. SAMPLING MODULE

9.2.1. Needle replacement
Perform needle replacement as is describe in the User’s Manual.
9.2.2. Complete rocker module replacement
- Before replacing the complete Sampling Module, drain all the tubing :
x Remove the diluent, lyse and cleaner tubes from their bottle
x Perform a PRIME PACK (MAIN MENU / MAINTENANCE / REAGENT PACK)
- Switch the D3 OFF, and disconnect the power supply.
- Remove the Reagent Pack bottle from it’s location
- Remove the upper cover (three screws) from the D3.
- Unscrew the rocker fixation screw but DO NOT remove it.
- Unscrew and remove the screw which holds the Needle Home
Position Sensor.
- Disconnect the three tubing and cut the Tie-wraps.
- Loosen the two guide screws One Turn and DO NOT remove
them.
- Push down the guide
- Pull to remove the complete rocker module.
REASSEMBLY PROCEDURE :
To reassemble the unit, carry out the various operations in reverse order.
*Warning* Do Not overtighten fasteners. The Threads are plastic and will be
damaged from overtightening.

REF : D3-SM-000 Rev 01 9. REPAIRING
9.3. COUNTING BATHS MODULE

9.3.1. Baths dismantling
Perform baths dismantling as described in Chapter 9 of the User’s Manual.
9.3.2. Baths o-ring replacement

Perform baths o-ring replacement as described in Chapter 9 of the User’s Manual.
9.3.3. Aperture block replacement

Perform aperture block replacement as described in Chapter 9 of the User’s Manual.
9.3.4. Complete baths module replacement
- Before replacing the complete baths module, drain all the

tubing : **The Cuvettes MUST BE DRAINED **
x Remove the diluent, lyse and cleaner tubes from their
bottle
x Perform a PRIME PACK (MAIN MENU /
MAINTENANCE / REAGENT PACK / PRIME PACK)
- Switch the D3 OFF and disconnect the power supply.
- Remove the Pack from it’s location
- Place the D3 on its left side.
Remove the baths connection (HGB, WBC coaxial, RBC coaxial

and thermal coupler).
Loosen (but DO NOT remove) the three screws which hold

the baths module (the third screw is located behind the over
flow tubing).
Cut the tie wraps off the tubing.
Disconnect the tubing from the manifold and the bath.
Slide up and remove the baths module.
Disconnect the ground wire from the chassis.
NOTE : Be careful with the tubing reconnection, if necessary, cut few millimeters (2 to 3mm) of tubing or
exchange them, ensuring a snug fit.
9.4. SYRINGE MODULE

9.4.1. Piston greasing
Perform the piston greasing as described in Chapter 9 of the User’s Manual.
9.4.2. O-ring or piston replacement
- Before replacing the complete syringe module, drain all the

tubing :
x Remove the diluent, lyse and cleaner straw from their
bottle
x Perform a PRIME PACK (MAIN MENU / MAINTENACE
/ REAGENT PACK / PRIME PACK)
- Put the syringe in lubrication position (MAIN MENU /
SERVICE / MECHANICS / LUBRICATE POS.)
- Switch the D3 OFF and disconnect the power supply.
- Remove the Pack from it’s location
- Place the D3 on its left side.
** THE CUVETTES MUST BE DRAINED **
- Loosen (but DO NOT remove) the four screws which hold the
syringe module.
- Pull the Block forward and rotate to gain access to the pistons.
Remove the syringe pistons from the piston carriage.
- Replace the pistons if needed. Replace O-rings.

To Replace O-Rings-
-.Unscrew and remove the eight screws which maintain the o-ring
plate.
- Remove the o-ring plate and exchange the o-rings.
-Lubricate O-rings with Dow Corning 101 silicone o-ring grease.
9.4.3. Complete syringe module replacement

Before exchanging the complete syringe module, put the D3 on its left side as describe above (section 9.4.2).
- Disconnect all the valves connection.
- Loosen (but DO NOT remove) the four screws which hold the
syringe module.
- Remove the waste tubing.
- Cut the tie wraps off the tubing.
--Unscrew and remove the screws which maintain the ground

fitting.
- Disconnect the tubing.

- Remove the complete syringe module.
NOTE : Before reattaching the tubing, cut a few millimeters (2 to 4mm) off the end of the tubing to ensure
a snug fit.
9.5. BOARDS REPLACEMENT
THE THREE D3 BOARDS ARE VERY

SENSITIVE TO ELECTROSTATIC
DISCHARGE. YOU MUST GROUND
YOURSELF USING AN BODY EARTH
GROUNDING STRAP TO HANDLE THE
BOARDS FOR THEIR REPLACEMENT OR
REMOVAL.
9.5.1. IHM board replacement
Loosen (but DO NOT remove) the

two upper screws which hold the
front panel.
Loosen (but DO NOT remove) the

lower screw which holds the front
panel.

Lift the front panel and then pull it toward the front of the
instrument.
Disconnect the flat cable from the IHM connector
Push out on the ends of the connector in order

to extract the flat cable.
9.5.2. Main board replacement
NOTE : Before removing the main board, remove the IHM board.
Push the flat cable through the window.
Remove the nine connectors located at the

top of the board. Label each connector and
record it’s position.
Unplug the baths connection (HGB, WBC coaxial, RBC coaxial and
temperature sensor).

Remove these three screws at the back of the instrument.
Remove the two screws located on the hydraulics side.
Disconnect the vacuum tubing, then carefully lift the

board up and out of the Analyser.
NOTE : Remember to reconnect the vacuum tubing after replacing the board.
9.5.3. Main board

9.5.3.1. Fuse replacement
- This fuse protects the 24v circuit.
- Remove the fuse with a thin pliers and replace it by a new one.
- Use a Littelfuse NANO² Slo-Blo fuse model 0454

004 to replace the old one.
- It’s strictly forbidden to use an another type of fuse
and will void warranty.
9.5.3.2. Battery replacement
- This battery is only used to supply the real time clock.
- With a plastic screwdriver, lift the clips holding the battery in

place.
- Slide down the battery to remove it and replace it.
- Use a Maxell battery model CR2016 to replace

the old one.
- It’s strictly forbidden to use an another type of
battery and will void warranty.

READ CAREFULLY THE FOLLOWING RECOMMENDATIONS.
Handling/storage
Never expose the battery to ultrasonic sound.
Exposing the battery to ultrasonic sound may cause short-circuiting because the inside material is broken
into pieces, leading to distortion, leakage, overheating, explosion, or fire.
Never subject the battery to severe shock.
Dropping, throwing or stomping on the battery may cause distortion, leakage, overheating, explosion, or fire.
Never short-circuit the battery while installing into equipment.

Please be careful when installing the battery not to short-circuit it with metal portions of the equipment.
Use the correct battery suitable for the equipment.
The battery may not be suitable for the specific equipment due to the using conditions or type of equipment.
Please select the suitable battery according to the handling instructions of the equipment.
Never use or leave the battery in a hot place such as under the direct rays of the sun or in a car in
hot weather.
If you do, this may cause distortion, leakage, overheating, explosion, or fire.
Never allow the battery to come in contact with water.
If it does, this may cause the battery to rust or lead to distortion, leakage, overheating, explosion, or fire.
Never store the battery in a hot and highly humid environment.
Doing so may cause the performance of the battery to deteriorate. In certain environments, this may lead to
distortion, leakage, overheating, explosion, or fire.
Disposal
The battery may be regulated by national or local regulation. Please follow the instructions of proper
regulation. As electric capacity is left in a discarded battery and it comes into contact with other metals, it
could lead to distortion, leakage, overheating, or explosion, so make sure to cover the (+) and (-) terminals
with friction tape or some other insulator before disposal.
9.5.4. Preamplifier board replacement
NOTE : Before removing the preamplifier board, remove the Main board.
Remove the two screws located on the copper side.
Gently lift the preamplifier box from the main

board while ensuring the finger connection is not
bent.
To reassemble the part, carry out the various operations in reverse order.

10. TROUBLESHOOTING
10. TROUBLESHOOTING
In the case of any problem not being solved, call the Drew Scientific distributor.
10.1. ANALYTICAL PROBLEMS

PARAMETERS PROBLEMS SYMPTOM SOLUTIONS
WBC No results No HGB Check the cuvette wires
Check the lyse and diluent levels
Check the right lyse tubing connection to the WBC
bath.
HGB OK Check the cuvette wires
Perform a Cleaning Cycle and then a Bleach cycle if
unsuccessful.
Bad Perform a Back flush and a Cleaning Cycle and then a
reproducibility Bleach cycle if unsuccessful
Check the presence of large bubbles during mixing in
the WBC bath during the run cycle.
RBC No results No HCT & PLT Check the cuvette wires
Perform a Cleaning Cycle and then a Bleach cycle if
unsuccessful
Bad HCT & PLT too Perform a Back flush and a Cleaning Cycle and then a
reproducibility Bleach cycle if unsuccessful
the RBC bath during the run cycle.
the WBC bath during the first dilution.
HGB No results Check the led light on
Bad Check if no bubble in the lyse tubing.
reproducibility Check the level bubble flow in the WBC bath during
the run cycle.
10.2. OTHER PROBLEMS

ORIGIN PROBLEMS SOLUTIONS
D3 Diluent leaks around the Check the rinsing needle block (presence of clots) and clean
needle during the run cycle it if necessary see section 9 of the Operators Manual.
No starting Check the power supply connection wires
All results bad Check the level diluent and if the tubing is pinched.
No display Check the flat cable between the IHM and Main pcb.
Printer No printing Check the paper.
Check the electrical connection.
Bad printing Check the black ribbon or toner cartridge.
10. TROUBLESHOOTING REF : D3-SM-000 Rev 01
10.3. TROUBLESHOOTING MESSAGE

This section give guidance as to know what to do when a troubleshooting message appears on the screen.
MESSAGE ACTION
*** N/A
SYSTEM: FATAL ERROR Re-start the Analyser.
SYSTEM : INTERNAL TIME OUT Re-start the Analyser.
CYCLE : BUSY Wait before performing a cycle.
CYCLE STOPPED BY USER Emergency stop, please perform a reset cycle.
CYCLE : EMERGENCY STOP Perform a reset cycle.
CYCLE: BAD CYCLE MODULE Perform a reset cycle.
CYCLE : CMD VALVE FAILED Change the valve
CYCLE : VALVE 1 FAILED Change the valve
CYCLE : INIT NOT DONE Perform an initialization or a reset cycle.
OUT OF RANGE Modify the value
MECA: MOTOR NEEDLE BUSY Re-start the Analyser.
MECA: MOTOR SYRINGE BUSY Re-start the Analyser.
MECA: MOTOR ROCKER BUSY Re-start the Analyser.
MECA : HOME NEEDLE NOT FOUND Perform an initialization or a reset cycle.
MECA : HOME SYRINGE NOT FOUND Perform an initialization or a reset cycle.
MECA : HOME ROCKER NOT FOUND Perform an initialization or a reset cycle.
MECA : MOTOR NEEDLE GAP Nothing.
MECA : MOTOR SYRINGE GAP Nothing.
MECA : MOTOR ROCKER GAP Nothing.
MECA : NEEDLE NOT IN TOP POSITION Perform a reset cycle.
CONTROL CYCLE NOT DONE Perform a reset cycle.
BACKGROUND NOT DONE Perform a background cycle.
RINSE NOT DONE Perform a clean cycle.
CLEAN NOT DONE Perform a rinse cycle.
BACKGROUND FAILED Perform a new background cycle
CYCLE: DOOR OPENED Close the door and perform an reset cycle
INTERN : COUNT ERROR Re-start the Analyser.
RUNNING AUTO CLEANING , Press OK.
REAGENT:PACK ALMOST EMPTY Replace the pack and perform a prime all

REF : D3-SM-000 Rev 01 10. TROUBLESHOOTING
MESSAGE ACTION
THE REAGENT PACK IS OUT OF DATE Replace the container and perform a prime all
SYSTEM : EEPROM COM ERROR Re-start the Analyser.
INIT PRINTER Switch on the printer or invalidate the printings.
NO PRINTER RESPONSE Switch on the printer or invalidate the printings.
NO PRINTER SELECTED Switch on the printer or invalidate the printings.
PRINTER IS OFF Switch on the printer or invalidate the printings.
NO PRINTER RESPONSE Switch on the printer or invalidate the printings.
PRINTER ERROR Switch on the printer or invalidate the printings.
Wait before performing a cycle. If persisting, re-start
INTERN: RESULT AREA IS LOCKED
the Analyser.
BACKUP: SYSTEM ERROR Re-start the Analyser.
HARDWARE: SYSTEM ERROR Re-start the Analyser.
BACKUP : MEMORY FULL. OLDEST RESULTS HAVE BEEN
Delete the stored results.
DELETED.
BACKUP : MEMORY IS ALMOST FULL. PLEASE ARCHIVE
NO PROBLEM. Information Only.
RESULTS
BACKUP : BAD FOLDER DUPLICATION Re-start the Analyser.
BACKUP : CALIBRATION HISTORY IS FULL Delete the calibration results.
BACKUP : REPEATABILITY HISTORY IS FULL Delete the repeatability results.
BACKUP : QC HISTORY IS FULL Delete the Q.C. results of the ongoing lot.
INTERN: NO MEMORY AVAILABLE Re-start the Analyser.
INTERN: MEMORY CORRUPTED Re-start the Analyser.
COM: CRC CONTROL ERROR Communication error retry
RS232: TIME OUT Re-start the Analyser.
RS232: INTERNAL ERROR Re-start the Analyser.
RS232: SYNCHRO ERROR Re-start the Analyser.
RS232: ACK ERROR Re-start the Analyser.
ID AND/OR PID MANDATORY ( CHECK SETUP). SID
Enter an ID and/or PID and SID
ALWAYS MANDATORY
CYCLE: PRESSURE DEFAULT ERROR Perform reset cycle
BACKUP : FOLDER NOT FOUND Re-start the Analyser.
BACKUP: LAST SETUP RESTORED. Indicate that your SETUP has been restored
BACKUP: SETUP UPDATED WITH DEFAULT VALUES. Indicate that your SETUP has been released
10. TROUBLESHOOTING REF : D3-SM-000 Rev 01
MESSAGE ACTION
BACKUP: PARTIAL NUM DELETED. A bad Result storage happened
BACKUP: PARTIAL CALI. RES. DELETED. A bad Result storage happened
BACKUP: PARTIAL REPET. RES. DELETED. A bad Result storage happened
BACKUP: PARTIAL QC RES. DELETED. A bad Result storage happened
BACKUP: LAST RESULTS RESTORED. File has been restored to previous state
BACKUP: LAST QC RESTORED. File has been restored to previous state
BACKUP: FILE SYSTEM FAILED Re-start the Analyser.
BACKUP: SECTOR FAILED hardware failure on memory
COM: BAD CYCLE MODULE Rebuild cycles with good options
COM: SIZE ERROR Send File again
SYSTEM : DOWNLOADING NEW VERSION… PLEASE
System is resetting after internal modifications
WAIT
USB:UNABLE TO OPEN FILE Read error. Try again. The key might have a problem.
USB:THUMB DRIVE IS FULL Insert new key or delete some files.
USB:EMPTY FILE The item was not found on the key.
USB:THUMB DRIVE IS NOT PRESENT Connecter une cle USB au Analyser.
USB:WRITE PROTECTED FILE Modify the file's properties (security).
USB:TOO MANY FILES OPENED Please close files.
SETUP: MODIFICATION NOT ALLOWED. The access path need higher privilege to proceed.
REAGENT:DILUENT EMPTY. Change Pack
REAGENT:LYSE EMPTY. Change Pack
REAGENT:CLEANER EMPTY. Change Pack
BACKUP: FIFO MODIFIED Re-start the Analyser.
End of document.

D3 Service Manual, Rev 1

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D3 Service Manual, Rev 1

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DREW-D3

The Americas Rest of the World

Revision Nb Date Author Software Comments

01 28/10/06 CM/JR >= V 1.0.2 First Release

DREW D3 Copyright© Drew Scientific. All Rights Reserved. Page 1/58

IMPORTANT SAFETY INSTRUCTIONS

RISK OF DANGER ! Indicates a procedure to be strictly followed in order to avoid

Indicates that wearing gloves is mandatory before performing the described

NOTE : Indicates important additional information.

OPERATORS MUST HAVE RECEIVED

BE CAREFUL WITH THE EDGES OF THE FRAMEWORK AFTER

Page 2/58 Copyright© Drew Scientific. All Rights Reserved. DREW D3

Guidance and manufacturer’s declaration – Electromagnetic immunity

Recommended separation distance

Conducted RF 3 Vrms 3 Vrms d = 1,2√P

Radiated RF 3 Vrms 3 Vrms d = 1,2√P 80MHz to 800MHz

Where P is the maximum output power rating of the

Field strengths from fixed RF transmitters, as

NOTE 1 At 80Mhz and 800MHz, the higher frequency range applies.

DREW D3 Copyright© Drew Scientific. All Rights Reserved. Page 3/58

Page 4/58 Copyright© Drew Scientific. All Rights Reserved. DREW D3

8.2. CONCENTRATED CLEANING ..................................................................................................................................................... 41

DREW D3 Copyright© Drew Scientific. All Rights Reserved. Page 5/58

The following chapters describe the different technical parts of the D3

Two dilution rates are performed :

2.2. FLUIDICS SEQUENCES DESCRIPTION :

RBC DILUTION AND LYSING :

DRAIN AND RINSE BATH :

NEEDLE BACK AND WASTE DRAIN :

The D3 is ready to perform a new analysis.

2.3. FLUIDICS VIEW

DREW D3 Copyright© Drew Scientific. All Rights Reserved. Page 9/58

2.4. FLUIDICS PARTS DESCRIPTION

2.4.1. Sampling module

2.4.2. Counting bath module

2.4.3. Syringes module

One motor drives the five syringes.

2.5. FLUIDICS DIAGRAM

DREW D3 Copyright© Drew Scientific. All Rights Reserved. Page 11/58

2.5.2. Manifold tubing and valves position

Counting manifold tubing position

Counting manifold valve positions

Syringe manifold tubing position

Syringe body tubing position

DREW D3 Copyright© Drew Scientific. All Rights Reserved. Page 13/58

Syringe manifold valves position

2.6. SIZE & LENGTH TUBING

Tygon Tygon Tygon Tygon Tygon Tygon

DREW D3 Copyright© Drew Scientific. All Rights Reserved. Page 15/58

3.2. ELECTRONICS PARTS VIEW

3.2.2. IHM board

3.2.3. Preamplifier board

DREW D3 Copyright© Drew Scientific. All Rights Reserved. Page 17/58

3.3. ELECTRONIC BLOCK DIAGRAM

Page 18/58 Copyright© Drew Scientific All Rights Reserved. DREW D3

3.4. WIRING DIAGRAM

DREW D3 Copyright© Drew Scientific. All Rights Reserved. Page 19/58

4.1. GENERAL FLAGS

4.2. LEUKOCYTE FLAGS

CL2-2 CL2 CL2-3 CL4 CL5

FLAG CHANNELS MEANING % VALUE # VALUE

4.3. ERYTHROCYTE AND HGB FLAGS

FLAG CHANNELS MEANING % VALUE # VALUE

4.4. PLATELET FLAGS