Jorge Bravo

CAMPOSUDLAP
April 13, 2020

LNM2012 3rd Partial Exam 95.45% (42/44)

1. TEM stands for Transparent Electron Microscopy

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F False

2. Samples need to be conductive to be observed in TEM

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F False

3. In a TEM the electron beam is focused using optical lenses

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F False

4. The maximum thickness to observe samples in TEM is

A no value restriction

B 1000 nm

C 10 nm

D 100 nm

E 100 micrometers

5. The maximum thickness to observe samples in SEM is

A no value restriction

B 1000 nm

C 10 nm

D 100 nm

E 100 micrometers
6. TEM and SEM both use basically the same electron voltages
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F False

7. Characteristic X-rays are induced due to the interactions of energetic electrons with the
sample within the SEM
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F False

8. Non-conductive samples can be coated with a metallic layer to be observed in SEM

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F False

9. SEM allows you to determine the internal structure of a sample

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F False

10. SEM allows you to image wet and non conductive samples
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F False

11. SPM stands for Slow Probe Microscopy

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F False

12. STM is a type of SPM

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F False

13. STM measures

A the electrons flowing from the tip to the sample substrate

B the force that the sample exerts on the tip

C the magnetic field generated with the tip and the sample
14. In AFM, the deflection of the cantilever is recorded by an optical method using the reflection of
a laser beam
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F False

15. This image represents

A IBM written with play-doh

B Xe atoms on top of nickel atoms

C Metallic clusters on top of silicon

16. A prokaryote is a primitive organism consisting of a single cell

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F False

17. In prokaryotes all the intracellular components are divided in cellular compartments

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F False

18. Name of bacteria that can produce magnetic nanocrystals

A magnetic bacteria

B magnetosomic bacteria

C magnetotactic bacteria

D magnetite bacteria

E magnetotaxis

19. Select the magnetic compounds

A MgO

B Fe3O4

C Fe3S4

D Fe2O3

E FeO

F FeS
20. Motile means
A self-propelled

B with motifs or patterns

C broken or cut

21. Magnetosomes are

A magnetic fields

B magnetic organisms

C magnetic nanocrystals

D magnetic environments

22. Viruses are used as scaffolds or templates to fabricate metalized nanoparticles and nanotube
structures using mineralization techniques
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F False

23. Eukaryotic cells contain membrane-bound organelles and nucleus

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F False

24. Eukaryotes comprehend unicellular and multicellular organisms

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F False

25. Algae have chlorophyll and produce approximately 10% of all oxygen today
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F False

26. Phyto-synthesis of nanomaterials consists of the synthesis using plants or plant extracts
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F False
27. Mention 3 principles of green chemistry

Catalysis
Atom economy
Design for energy efficiency

28. A dual beam microscope consists of

A SEM and TEM

B SEM and AFM

C AFM and TEM

D FIB and SEM

E STM and AFM

29. Low current used in the focused ion beam is used for sputtering or milling
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F False

30. Inside a FIB platinum is deposited thanks to a CVD reaction

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F False

31. Enlist the 5 basic steps of preparation of biological samples for TEM observation

Fixation
Dehydration
Embedding
Sectioning
Staining

32. Basic instrumental tool used for thin sectioning in TEM sample preparation of biological
samples
A resin embedding

B ultramicrotome

C microscope

D glass blade
33. Although TEM does not provide chemical information, the composition of a sample can be
inferred by measuring the interlayer distance of planes in a crystalline material
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F False

34. SEM can give information about the internal structure of a material
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F False

35. AFM carried out in vacuum can achieve atomic resolution

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F False

36. The image shown corresponds to this technique (image

credit:www.gatan.com)
A TEM

B SEM

C AFM

D STM

37. The figure contains images acquired with this technique (image
credit:DOI 10.1088/0957 4484/20/30/305602
A TEM

B SEM

38. Atomic manipulation was made posible thanks to the development of this technique
A TEM

B SEM

C STM

D FIB
39. In a quantum corral the circular patterns observed correspond to the constructive interference
of the electrons on the copper surface which are trapped inside the circle formed by the iron
atoms
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F False

40. Read the following extract of the synthesis methodology of Ag nanoparticles

(https://doi.org/10.1021/acs.langmuir.5b03081 and answer the following question (the same
extract will be used to answer two questions):

Chlorella pyrenoidosa was procured from National Center for Industrial Microorganism, Pune,
India. Batch cultures of C. pyrenoidosa were grown in Bold’s basal medium. Cultures in the
midexponential phase were taken from the culture flask into Falcon 50 mL Conical Centrifuge
Tubes Fisher Scientific) and centrifuged 5000 rpm, 5 min, 4 °C . The pellets were washed
thrice with deionized water to remove traces of media. A 50 mL portion of deionized water was
added in 1.5 g of wet algal biomass and kept at 100 °C for 5 min in an Erlenmeyer flask. This
was subsequently cooled at 27 °C followed by sonication for 5 min. After further centrifugation
at 5000 rpm for 10 min, the supernatant was filtered out with Whatman filter paper No. 1. The
pellet was discarded, and the supernatant was used as aqueous cell extract. Biosynthesis of
Ag NPs was carried out by addition of 10 mL of cell extract of C. pyrenoidosa in and 90 mL of 1
mM AgNO3 solution, followed by incubation at 28 ± 2 °C for 24 h. The change in color is
indicative of the reduction of AgNO3 to Ag NPs.

The synthesis reported here is using:

A yeast

B plants

C bacteria

D seashells

E algae
41. Read the following extract of the synthesis methodology of Ag nanoparticles: Chlorella
pyrenoidosa was procured from National Center for Industrial Microorganism, Pune, India.
Batch cultures of C. pyrenoidosa were grown in Bold’s basal medium. Cultures in the
midexponential phase were taken from the culture flask into Falcon 50 mL Conical Centrifuge
Tubes Fisher Scientific) and centrifuged 5000 rpm, 5 min, 4 °C . The pellets were washed
thrice with deionized water to remove traces of media. A 50 mL portion of deionized water was
added in 1.5 g of wet algal biomass and kept at 100 °C for 5 min in an Erlenmeyer flask. This
was subsequently cooled at 27 °C followed by sonication for 5 min. After further centrifugation
at 5000 rpm for 10 min, the supernatant was filtered out with Whatman filter paper No. 1. The
pellet was discarded, and the supernatant was used as aqueous cell extract. Biosynthesis of
Ag NPs was carried out by addition of 10 mL of cell extract of C. pyrenoidosa in and 90 mL of 1
mM AgNO3 solution, followed by incubation at 28 ± 2 °C for 24 h. The change in color is
indicative of the reduction of AgNO3 to Ag NPs. The microorganism is kept alive during the
synthesis of Ag nanoparticles
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F False

42. Read the following extract and answer the question below: Huang et al. 21 used oolong tea
extract for synthesizing iron nanoparticles. The polyphenol/caffeine content of the extract
served as the reducing and capping agent. Characterization by XRD and FTIR indicated that
zero valent iron, maghemite, and magnetite nanoparticles were present. The synthesis was
carried out using
A a tea plant

B a tea plant extract

C a tea root

D tea leaves

43. Read the following extract and answer the question below: Huang et al. 21 used oolong tea
extract for synthesizing iron nanoparticles. The polyphenol/caffeine content of the extract
served as the reducing and capping agent. Characterization by XRD and FTIR indicated that
zero valent iron, maghemite, and magnetite nanoparticles were present. Tea extracts played a
single role (reduction of the iron precursor) in the synthesis
T True

F False
44. Read the following extract and answer the question below: A novel method for the synthesis of
high-active-surface-area, platinum–tobacco mosaic virus Pt–TMV nanotubes is presented.
The Pt–TMV nanotubes were synthesized through methanol-driven reduction of sodium
tetrachloroplatinate(II) hydrate in the TMV solution. TMV was first dialyzed against 2 L of Milli-
Q water using a Slide-A Lyzer 10 k MWCO dialysis cassette Pierce Biotechnology). For the
platinum deposition onto the virus surface a 10mL suspension of TMV in water 0.02mg/mL
was mixed with 200mL of freshly prepared 2.5mM Na2PtCl4 Aldrich) in water. After 5 minutes
HPLC grade methanol was added to make up 4mL of solution. The color of the solution, initially
pale yellow/brown, changed to dark gray after around 24 h, indicating the end of the reduction
process. No stabilizing agents were used. The microorganism used in this synthesis is
A yeast

B virus

C bacteria

D fungi

45. Read the following extract and answer below: A novel method for the synthesis of high-active-
surface-area, platinum–tobacco mosaic virus Pt–TMV nanotubes is presented. The Pt–TMV
nanotubes were synthesized through methanol-driven reduction of sodium
tetrachloroplatinate(II) hydrate in the TMV solution. TMV was first dialyzed against 2 L of Milli-
Q water using a Slide-A Lyzer 10 k MWCO dialysis cassette Pierce Biotechnology). For the
platinum deposition onto the virus surface a 10mL suspension of TMV in water 0.02mg/mL
was mixed with 200mL of freshly prepared 2.5mM Na2PtCl4 Aldrich) in water. After 5 minutes
HPLC grade methanol was added to make up 4mL of solution. The color of the solution, initially
pale yellow/brown, changed to dark gray after around 24 h, indicating the end of the reduction
process. No stabilizing agents were used. The microorganism serves as reducing agent
T True

F False

46. Read the following extract and answer below: A novel method for the synthesis of high-active-
surface-area, platinum–tobacco mosaic virus Pt–TMV nanotubes is presented. The Pt–TMV
nanotubes were synthesized through methanol-driven reduction of sodium
tetrachloroplatinate(II) hydrate in the TMV solution. TMV was first dialyzed against 2 L of Milli-
Q water using a Slide-A Lyzer 10 k MWCO dialysis cassette Pierce Biotechnology). For the
platinum deposition onto the virus surface a 10mL suspension of TMV in water 0.02mg/mL
was mixed with 200mL of freshly prepared 2.5mM Na2PtCl4 Aldrich) in water. After 5 minutes
HPLC grade methanol was added to make up 4mL of solution. The color of the solution, initially
pale yellow/brown, changed to dark gray after around 24 h, indicating the end of the reduction
process. No stabilizing agents were used. The microorganism serves as template for the
formation of Pt nanotubes
T True

F False