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Datpeng Antibiotik Mikro-3
Datpeng Antibiotik Mikro-3
5.2 Perhitungan
5.2.1 Perhitungan Media
Pembuatan Media Nutrien Agar 200 ml
% komposisi NA ¿ 20 g/L
20 g xg
=
1000 ml 200 ml
20 g × 200 ml
x ¿ ¿4g
1000 ml
5.2.2 Perhitungan Pengenceran
5.2.3 Perhitungan Kelompok 1-7
Kelompok 1
1. Perhitungan Konsetrasi (S1, S2, S3, S4, S5)
μg
Diketahui Konsentrasi S3 = 100
ml
Perbandingan Konsentrasi = 4 : 5
S2
S2 : S3
4 5
=
x 100
μg
= 80
ml
S4
S4 : S3
4 5
=
x 100
μg
= 125
ml
S5
S4 : S5
4 5
=
125 X
μg
= 156,25
ml
S1
S1 : S2
4 5
=
X 80
μg
= 64
ml
S1 : S2 : S3 : S4 : S5
S2 = y2 + (y3T – y32)
Kelompok 1D
S2 = y2 + (y3T – y32)
S2 = 9,06 + ( 10,72 – 10,53)
S2 = 9,25 mm
Kelompok 2D
S2 = y2 + (y3T – y32)
S2 = 5,35 + ( 9,87 – 9,06)
S2 = 6,16 mm
Kelompok 3D
S2 = y2 + (y3T – y32)
S2 = 0 + ( 0 – 0)
S2 = 0 mm
Kelompok 4D
S2 = y2 + (y3T – y32)
S2 = 0 + ( 0 – 0)
S2 = 0 mm
9,25+6,16+0+ 0
S2 rata-rata = = 3,85 mm
4
S3 = y3 + (y3T – y33) = 3T
Kelompok 1D
12,53+ 10,53+10,13+10,5+9,9
S3T = = 10,72 mm
5
Kelompok 2D
Kelompok 3D
0+0+0+ 0+0
S3T = = 0 mm
5
Kelompok 4D
0+0+0+ 0+0
S3T = = 0 mm
5
10,72+ 9,87+0+ 0
S3 rata-rata = = 5,15 mm
4
S4 = y4 + (y3T – y34)
Kelompok 1D
S4 = y4 + (y3T – y34)
S4 = 12,5 + ( 10,72 – 10,13)
S4 = 13,09 mm
Kelompok 2D
S4 = y4 + (y3T – y34)
S4 = 10,28 + ( 9,87 – 9,68)
S4 = 10,47 mm
Kelompok 3D
S4 = y4 + (y3T – y34)
S4 = 0 + ( 0 – 0)
S4 = 0 mm
Kelompok 4D
S4 = y4 + (y3T – y34)
S4 = 1,77 + ( 0 – 0)
S4 = 1,77 mm
13,09+ 10,47+0+1,77
S4 rata-rata = = 6,33 mm
4
S5 = y5 + (y3T – y35)
Kelompok 1D
S5 = y5 + (y3T – y35)
S5 = 13,3 + ( 10,72 – 10,5)
S5 = 13,52 mm
Kelompok 2D
S5 = y5 + (y3T – y35)
S5 = 11,32 + ( 9,87 – 10,03)
S5 = 11,16 mm
Kelompok 3D
S5 = y5 + (y3T – y35)
S5 = 0 + ( 0 – 0)
S5 = 0 mm
Kelompok 4D
S5 = y5 + (y3T – y35)
S5 = 3,29 + ( 0 – 0)
S5 = 3,29 mm
13,52+11,16 +0+3,29
S5 rata-rata = = 6,99
4
mm
3. Persamaan Regresi Linier
x (log C) y (diameter hambat koreksi)
1,81 3,31
1,90 3,85
2 5,15
2,1 6,33
2,2 6,99
Keterangan:
x = log C (hasil point 1)
y = diameter hambat koreksi (hasil point 2)
a = -14,99 b = 10,05
y = bx ± a
= 10,05x – 14,99
4. Konsentrasi Koreksi
yu (koreksi) = yu + (ys – y3u)
keterangan:
yu = rata - rata diameter uji
ys = iterpolasi log S3 (subsitusikan log konsentrasi S3 ke persamaan regresi
linier yang diperoleh di point 3)
y3u = rata - rata diameter S3 cawan petri uji
yu seluruh cawan petri uji ampisilin
Su 1+ Su2+ Su 3+ Su 4
yu =
4
0+0+0+ 0
= =0
4
ys = bx ± a
b = 10,05
a = -14,99
x = log konsentrasi S3 (log 100 = 2)
ys = bx ± a
= 10,05 (2) – 14,99 = 5,11
y3u seluruh cawan petri uji ampisilin
Su 31+ Su32+ Su 33+Su 34
y3u =
4
9,9+9,9+ 0+0
= = 4,95
4
jadi yu (koreksi)
yu (koreksi) = yu + (ys – y3u)
= 0 + (5,11 – 4,95)
= 0,16
Xuji
y = bx ± a
y = yu (koreksi)
x = antilog konsentrasi uji
y = bx ± a
0,16 = 10,05x – 14,99
0,16 + 14,99 = 10,05x
15,15 = 10,05x
x = 1,50
Xuji = antilog X
= antilog 1,50
Iu
= 31,62
ml
Kelompok 2
Kelompok 3
Kelompok 4
Kelompok 5
Kelompok 6
Kelompok 7
5.3 Kurva
5.3.1 Kurva Kalibrasi Antibiotik Ampisilin
Kurva kalibrasi antibiotik ampisilin
8
7
6
Diameter hambat (mm)
5
4 Diameter hambat
3
2
1
0
1.81 1.9 2 2.1 2.19
log konsentrasi
6
5
Diameter hambat
4
3
2
1
0
1.81 1.9 2 2.1 2.19
log konsentrasi