Temp Lab A Average Time Standard Deviation

C Trial 1 Trial 2 Trial 3 seconds (s)

25 30.78 30.00 25.09 28.62 3.085
30 19.25 30.69 16.34 22.09 7.586
35 8.97 13.76 9.20 10.64 2.702
40 6.85 9.60 4.60 7.02 2.504
45 12.40 10.97 16.75 13.37 3.010
50 n/a n/a n/a n/a n/a
55 n/a n/a n/a n/a n/a

pH Lab B Average Time Standard Deviation

Trial 1 Trial 2 Trial 3 seconds (s)
4 n/a n/a n/a n/a n/a
5 n/a n/a n/a n/a n/a
6 n/a 22.88 24.50 23.69 1.146
7 22.10 19.70 18.60 20.13 1.790
8 14.47 31.66 n/a 23.07 12.155
9 n/a n/a n/a n/a n/a
10 n/a n/a n/a n/a n/a

Number of Substrates - 10 Seconds Average Standard Deviation

Substrate #
Trial 1 Trial 2 Trial 3 Substrate
1 4 4 2 3.3 1.15470053837925
2 6 8 6 6.7 1.15470053837925
3 6 8 8 7.3 1.15470053837925
4 8 12 10 10.0 2
5 12 16 14 14.0 2
Rate of Reaction

0.03494
0.04526
0.09396
0.14252
0.07478
n/a
n/a

Rate of Reaction

n/a
n/a
0.04221
0.04967
0.04336
n/a
n/a
 Lab A Graph - Effect of Temperature on Rate of Reaction of Amylase
0.16000

0.14000

0.12000
Rate of Reaction (1/Mean time)

0.10000

0.08000

0.06000

0.04000

0.02000

0.00000
20 25 30 35 40 45 50 55 60

Temperature (C)
 Lab B Graph - Effect of pH on Rate of Reaction of Amylase
0.052

0.05

0.048
Rate of Reaction (1/Mean time)

0.046

0.044

0.042

0.04

0.038
3 4 5 6 7 8 9 10 11

pH
 Lab C Graph - Substrate Concentration V Substrate Number
16.0

14.0

12.0
Initial Rate of Reaction (First 10 Seconds)

10.0

8.0

6.0

4.0

2.0

0.0
0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5

Substrate Concentration