-)

Question-? (60 minutes)

- You have been provided with three solutions of one carbohydrate. The concentrations are
as follows:

S1 is 1 mol dm-3; 52 is 0.5 mol dm-3; and 53 is 0.01 mol dm-3. Determine the effects of
these solutions on strips of the plarit material K1 provided.
Proceed as follows:
(r)
' Label four Petri dishes from 1 to 4. Half-fill each dish with one of the solutions as
shown in the table belLw, then replace the lids.
,,
Dish Solution

1 s1

2 S2

r(
$3

4 s3

cut off 5 mm from one end of the specirnen Kl provided and discard it. Now
cut off the next 3 cm length of K1 and hold it in a verticai position on the bench (see
diagram below). with a sharp scalpel cut the piece of KI longitudinally into two.
Then cut each half longitudinally. You now have fout strips, eich 3 cm iong.

filrl +--.
plane of
lii
first cut i il plane of
sec.nd cut
I it
LJ
Repeat this procedure with two further 3 cm lengths of K1 , so obtaining twelve
strips in all.
frrawing of one strip

Flace three of the strips into each of dishes 1, 3 and 3. Make sure that the
strips are under the surface of the solutions rather than floating'on the surface.
g0Yo ethanol
Heplace the lid*, Place the two rernaining strips into the tube of
with which you' have been supplied." Leave these'for five'minutes,' then
remove them and place them in Cish 4. Heplace the lid of this di$h, Leave the
four dishes for at least fifteen minutes while you continue with the rest'of
the question.
t/ore.)iii o;B!'not *irtt*, ii''tA'*; dishestre lert'Tof'iinri;J'rftfit stfiinutes. i
( ir) Atter at least fifteen minutes remove the lids of the'Pe1iiTdibhHh'And observe
the strips carefully, Draw the strips in dishes 1,2 and 3 to show their shapes as
accurately as psssible. Label'the epidermis of each strip,
I
1' 2 and 3'
happoned to the strips in dishes
(iii) Explain as f ully as possible what has

(iv) Draw the slriPs in dish 4' Label the spidermis of eactr strip.

-Sfnps in dish 4

(v)Howdoyouaccountforthedifferencesbetweenthestripsindishes3an.:l4?

"""'i"""""

(vi)Whywasitimportantthatthelidswerekeptonallthedishesduring
this investigation?

(iii) What deduf;tion could be rnade fibout the water pstsntial

, did not,changs i1s,q!ap.e when It was.placed in a sstuiion
' r,i ,-; :,, i'i+
"'r- r

thiscarbohvOiat;?''''