ACIBADEM MEHMET ALI AYDINLAR

UNIVERSITY

MOLECULAR BIOLOGY AND GENETICS

DEPARTMENT
2022-2023 FALL

MBG 111
GENERAL CHEMISTRY LABORATORY
REPORT 02
UV VISIBLE SPECTROSCOPY

Submitted to: Asst. Prof. Tuğba Arzu Özal İldeniz

Deadline: 6 December 2022

Group Number: 05
Group Members
Ceylin Baykoç 221403022
Esra Akel 210101038
Nuri Sarı 210101006
ABSTRACT

1. INTRODUCTION

2. PRE-LAB QUESTIONS

1. What affects the absorbance of a solution?

The amount of light of a certain wavelength that inhibits a substance from going
through is measured as absorbance. There are two main factors that affect the absorbance:
 Concentration of a substance
 Path length

2. What are the UV and Visible wavelength ranges?

3. MATERIALS

 Micropipette
 Micropipette tips
 Test tubes
 Crystal violet (1 M)
 Test tubes
 Spectrophotometer
 Cuvette
 Distilled water (dH 2 O)

4. METHODS

1- 1 M of crystal violet stock solution was given in test tube by the laboratory assistants.
2- 5 different test tubes were prepared by diluting the concentration of solution taken in
each test tube by 1/2.
3- All test tubes were prepared by diluting the concentration and filled into
spectrophotometer cuvettes and placed into spectrophotometer compartment.
4- The solution of unknown concentration was added to the cuvette and put into the
spectrophotometer machine, and the absorbance value and molarity was measured.
5- Absorbance values are measured from cuvettes placed in the spectrophotometer.

5. CALCULATIONS

Stock tube => 1 M

First test tube => 0,5 M
Second test tube => 0,25 M
Third test tube => 0,125 M
Fourth test tube => 0,0625 M
Fifth test tube => 0,03125
Unknown test tube => 0,616754 M
 6. RESULT AND DATA

3 mL 3 mL 3 mL 3 mL 3 mL

1 M Crystal Violet 3 mL dH2O 3 mL dH2O 3 mL dH2O 3 mL dH2O 3 mL dH2O

stock solution

1:1 1:2 1:4 1:8 1:16 1:32

Figure 1.1:

Table 1.1:

2.5

2
f(x) = 2.1965218104138 x − 0.0217308467017309
R² = 0.999272797802706

1.5

0.5

0
0 0.2 0.4 0.6 0.8 1 1.2

7. DISCUSSION