Scribd Logo
Upload

Welcome to Scribd!

  • PCR Virtual Lab

    Uploaded by

    Alvand Hormozi
    167 views2 pages
    Polymerase Chain Reaction (PCR) is a technique used to amplify a specific region of DNA through repeated cycles of heating and cooling. The process involves heating the DNA to separate the strands, cooling to allow primers to anneal, and extending the primers with DNA polymerase. This cycling results in exponential amplification of the target DNA region. The technique allows for rapid amplification of specific genes and is useful for tasks like DNA fingerprinting and disease diagnosis.

    Original Description:

    Copyright

    © © All Rights Reserved

    Available Formats

    DOCX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    Polymerase Chain Reaction (PCR) is a technique used to amplify a specific region of DNA through repeated cycles of heating and cooling. The process involves heating the DNA to separate the strands, cooling to allow primers to anneal, and extending the primers with DNA polymerase. This cycling results in exponential amplification of the target DNA region. The technique allows for rapid amplification of specific genes and is useful for tasks like DNA fingerprinting and disease diagnosis.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    167 views2 pages

    PCR Virtual Lab

    Uploaded by

    Alvand Hormozi
    Polymerase Chain Reaction (PCR) is a technique used to amplify a specific region of DNA through repeated cycles of heating and cooling. The process involves heating the DNA to separate the strands, cooling to allow primers to anneal, and extending the primers with DNA polymerase. This cycling results in exponential amplification of the target DNA region. The technique allows for rapid amplification of specific genes and is useful for tasks like DNA fingerprinting and disease diagnosis.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 2

    PCR Virtual Lab

    Refer to the following links:


    https://www.youtube.com/watch?v=G4sEhNKoPT8

    http://learn.genetics.utah.edu/content/labs/pcr/
    Objective: In this virtual lab, you will identify the resources and process of Polymerase
    Chain Reaction or PCR. Introduction:
    1. How many base pairs are there in the human genome?
    approximately 3 billion
    2. What is PCR? What does it do?

    3. What are some of the advantages of PCR? Name 1 and explain.

    4. What is unique about the design of PCR tubes?

    6. What is the first thing you need to add to your PCR tube?

    7. What is the function of a “primer?”

    8. What does DNA Polymerase do?


    Inside of the Thermal cycler:
    9. In Cycle 1 at 95 Degrees Celsius, what happens to the
    DNA?

    10. In Cycle 1 at 50 Degrees Celsius, what anneals


    (attaches) to the DNA strands?

    11. In Cycle 1 at 72 Degrees Celsius, what is activated and


    what is added to the DNA strands?

    12. What are the end products of Cycles 2 and Cycles 3?

    13. Label the processes of DNA replication shown to the


    left.
    A
    B
    C

    Analysis and Conclusions


    14. Why do you think that everything on your lab bench had to be in ice?

    15. Why did the pipette change tips every time? What is this important.

    You might also like