TEST FOR URINARY PROTEIN AND GLUCOSE USING

COMMERCIALLY AVAILABLE REAGENT STRIPS

Description: Urine reagent strips currently provides a simple, rapid means for
performing medically significant chemical analysis of urine including pH ,
specific gravity, protein , glucose, ketomes, nitrite, leukocytes ,blood,
bilirubin and urobilinogen.

Principle: The reagent strips consist of chemical-impregnated absorbent pads

attached to a plastic strip. A color - producing chemical reaction takes
place when the absorbent pads comes in contact with urine. The reactions
are interpreted by comparing the color produced on the color pad within
the required time frame with a chart supplied by the manufacturer. Several
colors and intensities of a color for each substance being tested appear on
the chart. By careful comparison of the colors on the chart and the strip, a
semi- quantitative value of trace, 1+, 2+, 3+ and 4+ can be reported.

Purposes: (Brunzel, 2013)

1. To screen urine of pregnant women for protein which is associated with cases of
pre –eclampsia and eclampsia..
2. To screen for presence of urinary tract infection.
3. To screen urine of pregnant women for presence of glucose which is associated
with gestational diabetes
4. To screen for presence of diabetes mellitus
5. Aid in the detection of renal diseases and kidney damage.
6. Used for evaluating and monitoring of renal function.

Equipment:
● 5 ml. syringe
● Small test tube
● Urine sample
● Tissue paper / disposable absorbent pad
● Urine Reagent strips
● Good source of light

Assessment:

1. Identify the client and explain the purpose of urine chemical testing for protein
and glucose.
2. Determine if client has history of pregnancy induced hypertension, gestational
diabetes from previous pregnancies or if has family history of diabetes mellitus,
hypertension and kidney disease.
3. Assess client for signs and symptoms accompanying proteinuria and glucosuria.

TEST FOR URINARY PROTEIN AND GLUCOSE USING ICABANBAN

COMMERCIALLY AVAILABLE REAGENT STRIPS
Planning:

1. Gather all the necessary equipment and materials needed for the procedure.

Implementation:

Criteria Rationale
1. Performs hand washing and wears Wahing hands and taking standard
clean gloves. precautions prevent the spread of
infection. (Carter, 2012)
2. Labels the test tube with the To prevent interchanging of specimen.
corresponding specimen number. ( Stransinger, 2014)
3. Mix the urine sample well. Using a 5 Formed elements like red and white blood
ml. syringe, transfer 5 ml. of the cells sinks at the bottom and will be
specimen in a clean dry test tube. undetected in unmixed urine (Strasinger,
2014).
4. Without touching the reagent pads, Allowing the strip to remian in the urine
get the strip from the container. for an extended period will cause leaching
Competely but briefly dip the reagent of reagents from the reagent pads
strip in the urine sample. (Strasinger, 2014).
5. Remove excess urine from the strip Removing excess urine from reagent strip
by touching the strip on the side of prevents run–over between chemicals on
the tube as the strip is withdrawn adjacent pads producing distortion of colrs
from the tube. (Strasinger, 2014).
6. Blot the edge of the strip horizontally Blotting the edge of the strip on an
on a tissue paper or any disposable absorbent pad will ensure against run-over
absorbent pad. (Strasinger, 2014).
7. Wait the specified time amount of For the best semi- quantitative results, the
time for the reaction to occur. manufacturer’s stated time should be
followed. (Strasinger, 2014).
8. Holding the strip horizontally Holding the strip horizontally will prevent
Compare the color reaction of the run –over of chemical in adjacent pads.
strip pads to the manufacturer’s color A good light source is essential for
chart in good ligthing ( Do not let the accurate interpretation of color reactions.
strip touch the color chart) (Strasinger, 2014).
9. Record the result using semi- Documentation serves as a legal record
quantitative values (trace, 1+ to 4+) or (Temple and Johnson, 2015)
an estimate amount in milligrams /
100 ml. of urine.
10. Cleans and disposes all used To decrease transmission of
materials. microorganisms (Greenberg and Bowden,
2012)
11. Removes gloves and performs Washing hands and taking standard
personal hand hygiene. precautions prevent the spread of
infection. (Carter, 2012)

TEST FOR URINARY PROTEIN AND GLUCOSE USING ICABANBAN

COMMERCIALLY AVAILABLE REAGENT STRIPS
Evaluation:

1. Documents the result of the test and other pertinent findings based from data
obtained during the assessment of the client.

Nursing Considerations:

1. Urine reagent strips should be protected from deterioration caused by moisture,

volatile chemicals, heat and light.
2. Reagent strips are removed just prior to testing and the bottle should be resealed
immediately
3. Manufacturers recommend that the reagent strip be stored at room temperature
below 30 c but should not be refrigerated
4. Reagent strips should not be used past the expiration date
5. A visual inspection of the strip should be done each time a strip is used to detect
deterioration even though the strips may still be within the expiration date.
6. Care must be taken not to touch the strips when removing from the container.
7. Test reagents strips using positive and negative controls, a minimum of once
every 24 hours, when opening a new bottle, when there are questionnable results
and when there is concern about the integrity of the strips.
8. Interfering substances in the urine, technical carelessness and color blindness are
sources of errors when using urine reagent strips.

TEST FOR URINARY PROTEIN AND GLUCOSE USING ICABANBAN

COMMERCIALLY AVAILABLE REAGENT STRIPS
 CENTRO ESCOLAR UNIVERSITY
Manila * Makati * Malolos

SCHOOL OF NURSING

PROCEDURE CHECKLIST

Test For Urinary Protein And Glucose Using Commercially Available Reagent Strips
Name of Student Score

Year/Section/Group/Number

Rate the student's performance by checking the appropriate box using the following criteria:

5 - Excellent (Carries out procedures efficiently, systematically and independently/Personality trait is observed at all times)
4 - Very Satisfactory (Carries out procedures efficiently and systematically but requires minimal guidance and
supervision/Personality trait is observed at all times)
3 - Satisfactory (Carries out the procedures efficiently and systematically but requires moderate guidance and
supervision/Personality trait is observed at all times)
2 - Fair (Carries out the procedures efficiently and systematically but requires close guidance and supervision/Personality
trait is observed at all times)
1 - Poor (Carries out the procedures inefficiently, unsystematically even under close guidance and supervision/Personality
trait is observed at all times)
0 - Not done

Criteria 5 4 3 2 1 0
ASSESSMENT
1. Identify the client and explain the purpose of the test.
2. Determine if client has history of pregnancy induced
hypertension, gestational diabetes from previous
pregnancies or if has family history of diabetes mellitus,
hypertension and kidney disease.
3. Assess client for signs and symptoms accompanying
proteinuria and glucosuria.
PLANNING
1. Gather all the necessary equipment and materials needed
for the procedure.
1.1 5 ml. syringe
1.2 Small test tube
1.3 Urine sample
1.4 Tissue paper / disposable absorbent pad
1.5 Urine Reagent strips
1.6 Good source of light
IMPLEMENTATION
1. Performs hand washing and wears clean gloves.
2. Labels the test tube with the corresponding specimen
number.
3. Mix the urine sample well. Using a 5 ml. syringe, transfer
5 ml. of the specimen in a clean dry test tube.

Test For Urinary Protein And Glucose Using Commercially Available Reagent Strips
Rev. 11.00.2018
Page 1 of 2
 4. Without touching the reagent pads, get the strip from the
container. Competely but briefly dip the reagent strip in
the urine sample.
5. Remove excess urine from the strip by touching the strip
on the side of the tube as the strip is withdrawn from the
tube
Criteria 5 4 3 2 1 0
6. Blot the edge of the strip horizontally on a tissue paper or
any disposable absorbent pad.
7. Wait the specified time amount of time for the reaction to
occur.
8. Holding the strip horizontally Compare the color reaction
of the strip pads to the manufacturer’s color chart in good
ligthing ( Do not let the strip touch the color chart).
9. Record the result using semi- quantitative values( trace,
1+ to 4+) or an estimate amount in milligrams / 100 ml.
of urine.
10. Cleans and disposes all used materials.
11. Removes gloves and performs personal hand hygiene.
EVALUATION
1. Documents the result of the test and other pertinent
findings based from data obtained during the assessment
of the client.

Total Score:

Comments/Suggestions:

Computation: Total Score divided by (number of items) x 20 (factor) = _________________

*Total points shall be transmuted using the table for 100 pts. Passing cut-off point is 65.

Equivalent Numeric Grade: _______________

Interpretation: __________________________

Test For Urinary Protein And Glucose Using Commercially Available Reagent Strips
Rev. 11.00.2018
Page 2 of 2
Evaluator: Conforme:

Clinical Instructor's Student’s Signature Date:

Printed Name & Signature

Test For Urinary Protein And Glucose Using Commercially Available Reagent Strips
Rev. 11.00.2018
Page 3 of 2