2023

Lecturer: Heather Hendrickse

E-mail address: Hendricksehl@cput.ac.za

FACULTY OF HEALTH & WELLNESS SCIENCES

DEPARTMENT OF BIOMEDICAL SCIENCES

BHSc: MEDICAL LABORATORY

SCIENCE
STUDY GUIDE

IMMUNOHAEMATOLOGY 2
STUDY GUIDE AND
STUDENT/LECTURER CONTRACT
NQF LEVEL: 7
SUBJECT CODE: IMH200S
Credits: 12

Index Page
BHSc: MEDICAL LABORATORY SCIENCE 1
IMMUNOHAEMATOLOGY 2 STUDY GUIDE AND STUDENT/LECTURER CONTRACT 2023
 1. Introduction 2
2. Prerequisite subjects 2
3. Registration/Roles and responsibilities/Punctuality/Safety/Attendance 2
4. Assessment policy and regulations 3
5. E-learning/Study material 3
6. Teaching strategies 3
7. Specified outcomes 4
8. Knowledgebase 4
9. Assessment opportunities 4-5
10. Theory sessions/Tutorial sessions/Practical session 5-6
11. Syllabus/Specific outcomes/Teaching activities & Assessments to meet 6-9
the outcomes
12. Self-study 9

Dear Mainstream and Articulation Students,

Welcome to the 2023 Immunohaematology 2 (IMH200S) semester 1 course. Please email

me Hendricksehl@cput.ac.za during office hours if you have further questions after our
sessions regarding the IMH200S course – please expect a 48-hour response time due to
other CPUT courses/students I am responsible for.

It is recommended to have a 45-minute focus lecture session – the remaining IMH200S

timetabled sessions can focus on working through the tutorials in groups and/or guided by
an assigned Tutor. On a Wednesday we will be going over your answers to the tutorials –
please note that by studying the answers to the tutorials only will not sufficiently prepare you
for the assessments.

1. INTRODUCTION
The course aims to provide the student with the knowledge, insight, and skills required to
progress to the 4th year Immunohaematology specialization module and successfully write
the final 4th year Immunohaematology summative assessment. It also aims to ensure
students acquire knowledge of the concepts and laboratory techniques that are utilized in an
Immunohaematology laboratory.

2. PREREQUISITE SUBJECTS
Anatomy, Physiology and Disease, Immunology.

3. REGISTRATION/ROLES AND
RESPONSIBILITIES/PUNCTUALITY/SAFETY/ATTENDANCE
See your general study guide provided by your year coordinator.

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IMMUNOHAEMATOLOGY 2 STUDY GUIDE AND STUDENT/LECTURER CONTRACT 2023
4. ASSESSMENT POLICY AND REGULATIONS
Assessments are carried out following CPUT’s Assessment Policy which can be found in the
CPUT Academic Rules and Regulations guidebook for students as well as in the general
study guide. Dishonesty during any assessment will be viewed in a serious light and
disciplinary action will be taken. Dishonesty also includes plagiarism (see general study
guide provided by your year coordinator).

5. E-LEARNING/STUDY MATERIAL
The subject guide, student and lecturer contract and lesson plan, summarised course notes,
2 X free e-texts, practical manuals 1 & 2, pre-recorded PowerPoint presentations (lessons 1-
10) and any other relevant information/video links/articles will be available on the BlackBoard
platform under the relevant content folder.

All recommended sources are available in the library (Bellville campus); however, I have
provided two free e-texts on BlackBoard for further reading/reference if needed.

Textbooks
Title: Immunohaematology (Principles and Practices)
Author: Eva D. Quinley
Publisher: Lippincott Williams and Wilkins
Edition: Latest

Title: Basic and applied concepts of Immunology

Author: Kathy D. Blaney and Paula Howard
Publisher: Elsevier
Edition: Latest

Title: Basic and Applied Concepts of Blood Banking and Transfusion Practices
Author: Kathy D. Blaney and Paula Howard
Publisher: Elsevier
Edition: Latest

Other reading
1. Modern Blood Banking and Transfusion Practices: Denise Harmening
2. AABB Technical manual: American Association of Blood Banks
3. Standards for the Practice of Blood Transfusion in South Africa
4. Clinical Guidelines for the use of Blood Products in South Africa

6. TEACHING STRATEGIES
 Theory/Practical/Tutorial sessions according to the lesson plan schedule
 Learner Management System (BlackBoard)
 Self-acquisition of knowledge (journal articles/video links)
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IMMUNOHAEMATOLOGY 2 STUDY GUIDE AND STUDENT/LECTURER CONTRACT 2023
 7. SPECIFIED OUTCOMES

At the end of the course, the student will be able to apply the principles of laboratory Quality
Assurance and Good Laboratory Practice.

8. KNOWLEDGEBASE
The student will interpret and integrate laboratory tests in the discipline of
Immunohaematology following statutory requirements.
 Develop independent and critical thinking
 Enhance problem-solving skills
 Enhance subject knowledge
 Enhance students’ ideas

Please note that Immunohaematology 2 sessions will not continue through the CPUT
vacation period, public holidays, and weekends. However, the Articulation Students
will be informed by their coordinator if they are required to attend sessions over a
particular weekend at CPUT, Bellville Campus.

9. ASSESSMENT OPPORTUNITIES
Participation in ALL assessments is compulsory.
The course evaluation will be using:
Year mark
Test 1=15%
Test 2=25%
Test 3=25%
Final summative assessment (Exam)=35%
These assessments contribute 50% towards the final mark.
Assessments will include questions that assess knowledge and understanding of the subject. In
addition, questions will be included that requires the ability to apply knowledge, solve problems
and evaluate.

NB: THERE WILL BE NO RE-TESTS FOR THOSE FAILING ANY ASSESSMENTS.

“Absent assessment”
Should a student be absent for an assessment, they will be given an opportunity at the end of the
semester to write an ‘absent assessment’ provided bona fide documentation is provided (see the
general study guide provided by your year coordinator).
Please note that the “absent assessment” will be set on the entire syllabus.

Final summative assessment (FSA) - Exam

- contributes 35% towards the final mark for the subject
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IMMUNOHAEMATOLOGY 2 STUDY GUIDE AND STUDENT/LECTURER CONTRACT 2023
To pass this FSA, the candidate must obtain a sub-minimum mark of 40%.
Should the candidate not attain a sub-minimum mark of 40% in the FSA, they will be given a re-
evaluation opportunity provided they have attained an overall mark of 45% or above. The
candidate will be notified individually via a BlackBoard email as to whether they qualify for the re-
evaluation.

NB: OVERALL FINAL MARK MUST BE 50% AND ABOVE.

A distinction is awarded for the attainment of an overall mark of 75% and above.
The FSA is comprised of one 3-hour paper. The paper will be broadly based on the entire field of
the discipline of Immunohaematology as covered in the syllabus. The student will be required to
draw on all knowledge gained to date to answer the questions. Candidates may expect a mixture
of case studies, short questions, and multiple-choice questions.
The final mark is compiled as follows:
 Year mark 50%
 FSA 50%

10.
Theory/Tutorial session
The theory session will follow the Department of Biomedical Sciences timetable. Two free e-
texts can be found on BlackBoard for further reading (no need to purchase the latest edition
of the prescribed texts).
The tutorial session will be on a Wednesday – please see the session & assessment
schedule/lesson plan on BlackBoard. Please note that your tutorial answers will not be
marked and therefore no submission of tutorial answers will be required.

Practical session
The practical session will follow the Department of Biomedical Sciences practical roster for
the Mainstream students – the start date will be announced timeously on BlackBoard.
Please note that I am not responsible for the practical groups and therefore cannot make
any changes – please consult with Ms Santos should there be any practical clashes.

The Articulation group (Cape Town and National Students full-time in the
workplace/Laboratories) will be exempted from the practical session.

I have ordered the semi-automated equipment (Johannesburg), requested fully tested blood

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IMMUNOHAEMATOLOGY 2 STUDY GUIDE AND STUDENT/LECTURER CONTRACT 2023
samples (WCBS - negative test for HIV, HBV, and HCV) and ordered reagents/kits (Bio-Rad
Switzerland) which are costly, and the Mainstream Students are required to please attend
this particular practical session (please see the session & assessment schedule/lesson plan
on BlackBoard) where you will perform an ABO and Rh typing as well as an antibody
identification test on the blood sample provided (please see your practical manuals 1 & 2 on
BlackBoard). Please read through it before attending your practical session – the Articulation
Students are required to read the practical manuals 1 & 2 as well. There will be no wet
practical exam for IMH200S, however, interpretation of results and case scenarios will be
questioned in the assessments and can include methodology (practical manuals 1 & 2).
……………………………………………………

11. Syllabus/Specific outcomes/Teaching activities & Assessments to meet the

outcomes

1. Introduction to and Historical aspects of Immunohaematology

2. Donor selection and blood collection

3. Component therapy

4. ABO & Rh blood group system

5. Haemolytic disease of the newborn/foetus

6. Other blood group systems

7. Compatibility testing and automation

8. Hazards of transfusion

9. HLA & Molecular applications

10. Quality assurance in a blood transfusion laboratory

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IMMUNOHAEMATOLOGY 2 STUDY GUIDE AND STUDENT/LECTURER CONTRACT 2023
 Theme/topic Specific outcomes Teaching activities to meet the Assessments to
outcomes meet the
outcomes
Lecturer and course  Student/Lecturer agreement Introductory session: N/A
introduction  Mainstream Students:
Room D019
 Articulation Students
can have a listen to a
pre-recorded
PowerPoint
presentation on
BlackBoard if unable to
attend the Teams
Meeting
Lesson 1  The development of blood transfusion, particularly the major  Lecture 1  test 1
Introduction to and techniques and discoveries  Tutorial 1 Q&A
Historical aspects of
Immunohaematology
Lesson 2  The selection criteria and collection of blood donations,  Lecture 2  test 1
Donor selection and autologous and designated donations, apheresis, and stem cell  Tutorial 2 Q&A
blood collection donations according to criteria in the current Standards for
Practice of Blood Transfusion in SA
Lesson 3  The requirements for storage, shelf life, and transport  Lecture 3  test 1
Blood Component  The benefits in their use  Tutorial 3 Q&A
Therapy
Lesson 4  The rules of inheritance of the ABO & Rh blood group antigens.  Lecture 4  test 2
ABO & Rh blood group  Rh antibody properties and implication in pregnancy and  Tutorial 4 Q&A
system transfusion medicine  Practical

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IMMUNOHAEMATOLOGY 2 STUDY GUIDE AND STUDENT/LECTURER CONTRACT 2023
Lesson 5
Haemolytic disease of
the Newborn/Foetus
 Routine antenatal and post-natal sample testing, including  Lecture 5  test 2
samples in which antibodies are detected  Tutorial 5 Q&A
 Identification of irregular antibodies and the reason for the
titration of obstetrically significant antibodies
 The prevention of the disease
 Antenatal management of pregnant women with clinically
significant antibodies of high titre and a history of recurrent
miscarriage before 30 weeks gestation due to HDN/F
 Post-natal management of affected neonates

Lesson 6  The various blood group systems  Lecture 6  test 2

Other Blood group 1. Kell  Tutorial 6 Q&A
systems 2. Duffy  Practical
3. Kidd
4. MNSs
5. Lewis
6. P
7. I
- concerning:
o Detection and identification of antibodies
o Significance in transfusion medicine and pregnancy
Lesson 7  Patient identification procedures are carried out to minimize the risk  Lecture 7  test 2
Compatibility testing & of recipient mismatch from the time the sample is taken to the time of  Tutorial 7 Q&A
Automation infusion of the blood/blood component  Practical
 The various compatibility testing (ABO & Rh typing, Antibody screen &
identification, crossmatch, and phenotype) procedures, including the
selection of the correct donor blood/blood component including the
selection of blood/ blood component for babies suffering from HDN/F
 The use of automated equipment for compatibility testing
Lesson 8  Possible cause of a transfusion reaction  Lecture 8  test 3
Hazards of transfusion  The handling of a transfusion reaction  Tutorial 8 Q&A
 The importance of product recall/ “look-back’ programme - in the
event of disease transmission

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IMMUNOHAEMATOLOGY 2 STUDY GUIDE AND STUDENT/LECTURER CONTRACT 2023
 Lesson 9  The role of HLA in stem cell transplantation  Lecture 9  test 3
HLA & Molecular  Molecular applications in transfusion medicine  Tutorial 9 Q&A
applications - Blood group systems
- Stem cell transfusion
- Paternity testing
- Infectious disease testing
Lesson 10  The importance of a Quality Management System (QMS) in a blood  Lecture 10  test 3
Quality Assurance in a transfusion laboratory  Tutorial 10 Q&A
blood transfusion
laboratory

12. Self-study
Students are required to conduct self-study including the completion of all tutorials/viewing video links/reading uploaded articles.

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IMMUNOHAEMATOLOGY 2 STUDY GUIDE AND STUDENT/LECTURER CONTRACT 2023