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Table: MPN table for a three-replicate design from FDA’s Bacterial Analytical

Manual.
Positive Tubes
0.1 0.01 0.001 MPN 95% Confidence Range
0 0 0 <3.0 0-9.5
0 0 1 3 0.15-9.6
0 1 0 3 0.15-11
0 1 1 6.1 1.2-18
0 2 0 6.2 1.2-18
0 3 0 9.4 3.6-38
1 0 0 3.6 0.17-18
1 0 1 7.2 1.3-18
1 0 2 11 3.6-38
1 1 0 7.4 1.3-20
1 1 1 11 3.6-38
1 2 0 11 3.6-42
1 2 1 15 4.5-42
1 3 0 16 4.5-42
2 0 0 9.2 1.4-38
2 0 1 14 3.6-42
2 0 2 20 4.5-42
2 1 0 15 3.7-42
2 1 1 20 4.5-42
2 1 2 27 8.7-94
Table: MPN table for a three-replicate design from FDA’s Bacterial Analytical
Manual.
Positive Tubes
0.1 0.01 0.001 MPN 95% Confidence Range
2 2 0 21 4.5-42
2 2 1 28 8.7-94
2 2 2 35 8.7-94
2 3 0 29 8.7-94
2 3 1 36 8.7-94
3 0 0 23 4.6-94
3 0 1 38 8.7-110
3 0 2 64 17-180
3 1 0 43 9-180
3 1 1 75 17-200
3 1 2 120 37-420
3 1 3 160 40-420
3 2 0 93 18-420
3 2 1 150 37-420
3 2 2 210 40-430
3 2 3 290 90-1000
3 3 0 240 42-1000
3 3 1 460 90-2000
3 3 2 1100 180-4100
3 3 3 >1100 420-4000

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