The document summarizes a laboratory exercise observing the development of a 16 hour chick embryo under a microscope. Key structures that can be seen at this stage are the primitive streak, which appears as a thickening and will form the basis of the embryo body axis, Hensen's node at the cephalic end which contains closely packed cells, and the primitive groove and ridges. Around the area pellucida are the area opaca and primitive streak which will contribute to the formation of extraembryonic membranes and tissues. Photos also show the embryonic structures at 16 hours of incubation both with and without magnification.
The document summarizes a laboratory exercise observing the development of a 16 hour chick embryo under a microscope. Key structures that can be seen at this stage are the primitive streak, which appears as a thickening and will form the basis of the embryo body axis, Hensen's node at the cephalic end which contains closely packed cells, and the primitive groove and ridges. Around the area pellucida are the area opaca and primitive streak which will contribute to the formation of extraembryonic membranes and tissues. Photos also show the embryonic structures at 16 hours of incubation both with and without magnification.
The document summarizes a laboratory exercise observing the development of a 16 hour chick embryo under a microscope. Key structures that can be seen at this stage are the primitive streak, which appears as a thickening and will form the basis of the embryo body axis, Hensen's node at the cephalic end which contains closely packed cells, and the primitive groove and ridges. Around the area pellucida are the area opaca and primitive streak which will contribute to the formation of extraembryonic membranes and tissues. Photos also show the embryonic structures at 16 hours of incubation both with and without magnification.
16 hours Chick Embryo To access the parts of the egg, the shell must be broken by striking it gently with a blunt object, in this case, the handle of a fork is used. To have a vivid view, the contents of the egg are poured into a flat container. The observation begins upon completing these steps. If seen with the naked eye, the progress of the embryo can be difficult to identify. However, under a microscope, the primitive streak becomes visible. In fixed and stained slide, the parts of the embryo at its early development can be seen. The central furrow can be seen and is called as primitive groove lined by thickened primitive ridges. Located at the cephalic end of the primitive streak are closely packed cells that form thickened area called the Hensen’s node. A part of area pellucida adjacent to the primitive streak shows increased thickness and forms embryonic elliptical shape. The area pellucida assumes an elliptical shape. The elongated primitive streaks make up the long axis of the future embryonic body. Lastly, the caudal end of the streak that lies close to the area opaca which is the opaque area of the blastoderm surrounding the area pellucida. This helps in the formation of extra embryonic membrane such as yolk sac. As a summary, the parts of an embryo present at this stage (from cephalic end to caudal end) include the area opaca, area pellucida, Hensen’s node, primitive pit, primitive groove, and the primitive ridge.
Parts of the Embryo at 16 hours with label
Figure 1. Photo of actual embryonic development at 16 hours of incubation.
Figure 2. Photo of embryo at 16 hours under the microscope.
Documentation Link to the short clip: https://drive.google.com/file/d/1TFWTT2WgHT83cwfql0WnwWDBnm6YIX-U/view?usp=drivesdk