Microbial Genomics Genetic Engineering and Application of Bacterial Genetic Recombination

Biotechnology – Part 2 Events

Recombinant DNA technology

Recombinant DNA – invitro (manipulating the

DNA of a sample and combining it with a target
DNA to be manipulated)
 Combination of 2 DNA material
Restriction enzymes cut a specific portion of
plasmid, causing DNA to bind to it.
Example: Insulin
Molecular Cloning
Blue-White Screening - to determine if you had
produced recombinants

Transformation and Plasmids in bacteria

Insertion of gene from an organism into a

bacterial plasmid
Eukaryotic Cells and Recombination Agarose Gel Electrophoresis

Shuttle Vectors and Ti Plasmid

SDS – PAGE

Agrobacteriumtumefacions
Genomes
Polymerase Chain Reaction Recombinant DNA Technology and Gene
therapy

Sequencing