GENETIC ENGINEERING and

RECOMBINANT DNA

Genetic engineering, also called recombinant

DNA technology involves the group of
techniques used to cut up and join together
genetic material, especially DNA from different
biological species, and introduce the resulting
hybrid DNA into an organism to form new
combinations of heritable genetic material.

Genetic engineering refers to

the manipulation and PROCESSES INVOLVED IN
alteration of genetic GENETIC ENGINEERING
information. One procedure of

genetic engineering is DNA

1. Use of restriction
recombination. Genetic
enzymes
engineering and DNA
recombination are used mainly 2. Transformation and
to enhance the genetic cloning
condition of a particular 3. Cloning and screening
organism.
 Recombinant DNA (rDNA) refers to the genetic material
from two or more different species recombined into a
host organism. Using rDNA technology, scientists can
insert DNA into bacteria an dinto cells of plants and
animals. GENETIC ENGINEERING, also called rDNA
technology, involves the group of techniques used to cut
up and join together genetic material, especially DNA
from different biological species, and introduce the
resulting hybrid DNA into an organism to form new
combinations of heritable genetic material.

APPLICATIONS OF RECOMBINANT DNA

1. Applications in crop
improvement
2. Applications in medicine
3. Industrial application
NOTES:
Use of restriction enzymes - DNA is sliced and
separated by particular restriction enzymes, bacterial
enzymes that cut DNA at specific nucleotide
sequences. This process involves two significant
element. Vectors and Plasmids.
Transformation and cloning - the recombinant
plasmid is added to a bacterial colony that transforms
them and allows them to reproduce by binary fission
Cloning and screening - this stage involves the
isolation of cells that contain the gene of interest