Biology 2401 – Laboratory 6 report (Winter 2019)

One lab report per pair

Mark: / 7
Partner 1: Muhamed Sabirov Assigned patient: #10
Partner 2: Ralph Lopez Assigned patient: #9
Table 1 [2]: ELISA data

Well #’s and Predicted results Actual results Conclusion

Condition (colour) (colour)
1-3 (positive Blue Blue Positive reaction
control) (Biology Department,
2018).
5-7 (negative Colourless Colourless Negative reaction, no
control) serum antibodies
(Biology Department,
2018).
9-11 (patient Blue Positive reaction,
#9 sample) presence of serum
antibodies
(Biology Department,
2018).
9-11 (patient Blue Positive reaction,
#10 sample) presence of serum
antibodies
(Biology Department,
2018).

Table 2 [0]: Class data (refer to class data posted on Blackboard)

Questions:
1. [2] Q: Based on the class data, which medical procedure(s) was most likely the
cause of transmission of Hepatitis C?
A: According to the class data, patients 4,9,10 have been afflicted. It
appears that the most likelihood of hepatitis transmission is due to insulin
injection; As it was the only procedure shared among these 3 patients.
2. [1] Q: What would be the effect on the class data if the technician responsible
eliminated the last series of wash steps for all the samples?
A: All patient’s results would indicate false positive test results, which is
inaccurate outcome of the test (Biology Department, 2018).
3. [2] Q: It is recommended that after two months, all patients that tested negative

BIOL 2401 (Fall 2018) – Lab 6 Page 1 of 2

 should be re-tested. What is the main reason for this recommendation?
A: It is possible that these patients have not developed sufficient number of
antibodies against the hepatitis C virus for accurate detection by ELISA
test, therefore initial test results may come out to be false-negative;
However, a follow-up test would eliminate such uncertainty (Lori Smith,
2017).

Works cited:
Biology Department (2018). Introduction to Microbiology for Health Sciences:
Laboratory Manual. New Westminster, BC: Douglas College.
Lori Smith, (21 December, 2017), When to get tested for hepatitis C after exposure.
Retrieved from https://www.medicalnewstoday.com/articles/320375.php

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