Antibacterial Activity of Lemon

(Citrus limon) Peel Extract against

Staphylococcus aureus and
Escherichia coli

PRESENTED BY
Bethelen Jewel Gaviola
 Used to treat or prevent some
types of bacterial infection

diseases are becoming harder

and sometimes impossible to treat
as antibiotics become less effective
 Antibiotic resistance is a
growing concern globally.

Global health and food

security are under threat
involving billions of lives
Statement of the Problem
1 Does the lemon peel extract have anti-bacterial
property against S. aureus and E. coli?

What is the mean diameter of the average ZOI of

2 the lemon peel extract against S. aureus and E.
coli?

Is there a significant difference between the

3 average ZOI of the lemon peel extract and the
positive control in S. aureus and E. coli?
 Methodology Materials 2.1

Organic Material

Apparatus
462g of ripe lemon peels

Bottles
Reagents Beaker

Erlenmeyer flask
1L of 80% Ethanol Knife
Petri Dish
Equipments Reagent Bottle

Sterile cotton swab
Rotary evaporator
Methodology Research Design 2.2
Methodology Flowchart 2.3
Collection and Collection and
Plant Antibacterial
Preparation of Preparation
Extraction Screening
Samples of Samples

Adjusting the
Preparation of the Preparation of 0.5
Preparation turbidity of
Nutrient Broth Mcfarland Standard
of Plates the inoculum

Cotton Swabbing Paper Disc Measurement of Statistical

Disposal
on the Agar Plate Diffusion Method Zone of Inhibition Analysis
Result and discussion Antibacterial Screening

Table 2. Zone of inhibition of the treatment groups against S. aureus and E.

coli

Result and discussion Antibacterial Screening

Figure 2. Diameter of zone of Inhibition of the two treatments against S.

aureus
Result and discussion Antibacterial Screening

Figure 3. Diameter of zone of Inhibition of the two treatments against E. coli

Result and discussion
Table 3. Univariate Analysis of Variance of the Zone of Inhibition of the Plant
Result and discussion
Table 4. Post Hoc Analysis for the ANOVA Result (Scheffe)
Conclusion:

After the thorough analysis of the results, the following

conclusions were formulated:

1. The lemon peel extract contains anti-bacterial property

against S. aureus and E. coli.

2. The mean diameter of the average ZOI of the lemon peel extract
is 24mm against S. aureus and 26mm against E.coli.

3. There is a significant difference between the average ZOI

of the lemon peel extract and the positive control in S. aureus and E. coli.