M.Sc. Syllabus 2020-2022

2.
1.
SYLLABUS FOR
M.Sc. IN
BIOTECHNOLOGY
Choice Based Credit System (CBCS)
Revised Date : 23th October, 2017
(24th BOS Meeting)
DEPARTMENT OF BIOTECHNOLOGY
Hamdard University, (Jamia Hamdard)
New Delhi 110 062
www.jamiahamdard.ac.in
www.jamiahamdard.edu
Department of Biotechnology, SCLS, Jamia Hamdard 1
INTRODUCTION
VISION OF THE DEPARTMENT
The Department of Biotechnology at Jamia Hamdard, New Delhi was established in the
year 1997 with a vision to be recognised as a Department of International repute with a
strong interdisciplinary research and teaching base in Plant and Animal Biotechnology
with active collaboration of industries and health-care institutions.
RESEARCH AREAS
 Molecular biology and regulation of gene expression in infectious and non-

infectious diseases.
 Host pathogen interaction, molecular immunology, molecular virology and drug
delivery.
 Clinical virology and vaccine development.
 Development of diagnostic tests.
 Development of nano-enabled interfaces and multiplexed paper-based devices for
the detection of diseases.
 Genomics/ Proteomics of cancers and development of biomarkers for early
detection and valuable therapeutic targets.
 Conservation of medicinally important endangered plants through in vitro
approach.
 Enhancement in the yield of secondary metabolites in medicinal plants via stress,
culture conditions, genetic engineering and nanoparticles.
 Transgenics of vegetables, floriculture and oil crops.
 Evaluation of anti-Cancer potential of herbal based compounds and their nano
formulations.
 Stress Biology, mechanism of protein folding and stabilization.
INFRASTRUCTURE
Departmental Instrumentation Facility (DIF): DIF is well equipped with basic and
advanced instruments for training of students and research purpose. Weighing balance,
Ice flaking machine, Autoclaves, Millipore water system, Centrifuge, PCR,
spectrophotometer, Freeze dryer, Ultradeep freezer (-80˚C) Real time PCR, Rota
evaporator, GC-MS, Fluorescence Microscope, UV chamber, Shaking incubator and
Ultra-centrifuge.
Animal cell culture Facility: Animal cell culture facilities are well equipped with CO2
incubators (New Brunswick, Shell lab), water bath, cabinets and microscopes. We have
one well equipped dedicated cell culture facility for students training purpose. The
departmental cell culture facility is used by research scholars and faculty members.
Plant Tissue culture facility: A media preparation, sterilization, and storage area,
Laminar air flow chambers with HEPA filters, culture room with well-controlled
temperature, humidity, air circulation and light quality and duration is available for the
M.Sc. students and researchers. Besides, Plant Growth Chamber is available in the
Department. Hardening of in vitro raised plants is done in greenhouse by the
researchers.
BSL-II-B2 laboratory: Our department also have BSL-II facility well equipped with
Biosafety level-II B2 cabinet (Haier), CO2 incubator and microscope for infectious disease
and clinical studies. This infrastructure is established from the project funds of Dr.
Mairaj Ahmed Ansari.
Confocal microscopy: Our department is also having functional Confocal Laser

Scanning Microscope from Leica Microsystem for use of M.Sc. students and Ph.D.
Scholars of the Department as well as other Department and Schools of Jamia Hamdard.
Apart from above mentioned departmental common facilities, each faculty of the
department have their own well-equipped laboratories required for research purpose.
Besides, we are also availing FACS (BD) and nano-LC-MS (Waters) facilities available in
the University.
CENTRE FOR TRANSGENIC PLANT DEVELOPMENT:
Centre for Transgenic Plant Development as a unit of the Dept. of Biotechnology was
established in February 2004 with the research grants of CSIR (TMOP&M), Department
of Agriculture Cooperation and Farmers Welfare, Govt. of India and inaugurated by
Hon’ble Minister of Agriculture, Govt. of India, with a mandate to focus research in the
development of transgenic plants with improved yield and quality of produced as well as
development of DNA based finger prints for authentication of traditional medicines.
Various labs in the Centre have specialized equipments and facilities to work. It is
equipped with the state-of-the-art facilities to train Ph.D. and postdoctoral students and
to carry out research in various disciplines of Plant and Microbial Biotechnology. The
major R&D activities being pursued include cloning and characterization of novel genes
linked with tolerance to biotic and abiotic stresses and quality traits of medicinal and
crop plants, authentication and standardization of crude components of herbal
formulations and nano vehicle assisted gene delivery in plants. The thrust areas of
centre also include improving the quality of medicinal crops through genetic engineering
of metabolic pathway; miRNA and RNAi approaches, conservation of medicinal plants;
developments of easy, rapid, sensitive, cost effective method for aflatoxigenic mould
detection in the groundnut kernels and soil and identification and quantification of
aflatoxins in the food and feed. The centre always remains funded from Jamia Hamdard
and other government agencies such as DST, DBT, Department of AYUSH, CCRUM,
CSIR, ICMR, DRDO etc. for R&D projects carried out at the Centre. The consultancy
projects from biotech companies are also carried out in the centre.
EXTRAMURAL RESEARCH FUNDING
The faculty members of the Department have been able to attract number of
extramurally funded research projects from various national and international funding
agencies such as DBT, DST, SERB, CSIR, ICAR, ICMR, DRDO, UGC, DoEn, ISM&H,
CCRUM, AYUSH and World Bank.
UGC-Special Assistance Program
The SAP scheme was initiated in 1963 by University Grants Commission keeping in view
the recommendations of the Education Commission to facilitate the selected number of
University Departments having some potential in research and teaching. The programme
is intended to encourage the pursuit of excellence and teamwork in advanced teaching
and research to accelerate the realization of international standards in specific fields.
The Department of Biotechnology, Jamia Hamdard was selected under UGC-SAP (DRS-I)
from 2011 to 2016 with a sanctioned amount of Rs. 65 Lakhs. Again in 2017, seeing the
potential in research and teaching, Department of Biotechnology recommended to UGC-
SAP (DRS-II) from 2018 to 2022 with a sanctioned amount of Rs. 116 Lakhs.
DST - FIST
Universities are cradles of innovation and knowledge creation. Research in universities

has three-tier effects concerned with quality of under-graduate & postgraduate education
and value of research. They are the source of generating high caliber manpower and
repositories of national intellectual wealth in Science & Technology (S&T) sector, which if
channelized properly, may lead to socioeconomic development. In the emerging global
scenario, India can be an ideal destination due to its cost effectiveness and availability of
knowledge base. It must also aim for a permanent position in the field of Science &
Technology. Considering the present status of the S&T sector in the universities and
related academic institutions who are in dire need for strengthening the existing S&T
infrastructure support with adequate funding and associated flexibility, Government of
India in the year 2000 announced a major new initiative titled "Fund for Improvement of
S&T infrastructure in universities & higher educational institutions (FIST)" to rebuild the
Science & Technology infrastructure in the country. The quality of UG and PG education
and value of research, Department of Science and Technology, Govt. of India
recommended Department of Biotechnology, Jamia Hamdard under DST-FIST (Phase – I)
in 2019 and sanctioned amount of Rs. 80 Lakhs.
ADMINISTRATION
TEACHING STAFF
S.No Name Designation Specialization
Professor &
1 Dr. Pradip K. Chakraborti Molecular Biology
Head
Plant Biotechnology and

2 Dr. M.Z. Abdin Professor
Physiology
Molecular Virology,
3 Dr. Pratima Ray Professor
Vaccinology
4 Dr. Alka Narula Asst. Professor Plant Biotechnology
Protein Biochemistry and

5 Dr. Humaira Farooqi Asst. Professor
Stress Biology
Clinical Genomics and

6 Dr. Saima Wajid Asst. Professor Proteomics, Molecular
Oncology
Nano-enabled Interfaces for

7 Dr. Jagriti Narang Asst. Professor
Diagnostic Applications
8 Dr. Javaid Ahmad Sheikh Asst. Professor Immunology
Host Pathogen Interaction and

9 Dr. Mairaj Ahmed Ansari Asst. Professor
Molecular Immunology
INSA Senior Stress Physiology and

10 Dr. Renu Khanna Chopra
Scientist Fellow Biochemistry
Molecular Biology and

11 Dr. Asrar Ahmad Malik Guest Lecturer
Bioinformatics
FACULTY PROFILES
DR. ALKA NARULA
Specialization : Plant Biotechnology
Email : anarula@jamiahamdard.ac.in, alka.narula@rediffmail.com
Mobile : 9899000130
Contact No. : 011-26059688 (Extn : 5505)
Dr Alka Narula is Assistant Professor in the Department of Biotechnology. She

completed her Ph. D. in 2002. Dr Alka is a recipient of fast Track Project, Women
Scientist Scheme (DST project) as well as UGC major project. She has co-edited a
book entitled, “Plant Biotechnology and Molecular Markers” published by Kluwer
and Anamaya publisher. Dr Alka also has good number of research publications
(35) and book chapters (18) in reputed International and National Journals. She
has been awarded first prize in oral as well as poster presentations during various
Seminars/Symposia and Conferences. She was the Member of Organizing
Committee for Training in Tissue Culture, Transgenic Technology and
Biotechnology, Coordinator of Workshop on Plant Tissue Culture and
Improvement of Medicinal Plants, sponsored by DBT and XV International
Genetics Congress Trust, Member of Organizing Committee of National Seminar
on Proteomics based biomarkers for Animal and Plant Diseases at Jamia
Hamdard. Dr Narula as the Convener of UGC SAP (DRS-I) sponsored National
Seminar on Plant Biotechnology: Challenges and Opportunities in 21st Century,
conducted the event successfully and has recently worked as a Co-convener of
UGC SAP sponsored Seminar. She is Elected as member of PLANT TISSUE
CULTURE ASSOCIATION (India) in 2010. She has Supervised 64 M.Sc. Projects
and 20 Summer Trainees. Seven of her PhD students are awarded PhD degree in
2014 and some others are still involved in thesis writing or doing experimental
work. She is an editorial member of Journal of Enzymology and Metabolism.
Besides, she is also Referee of Indian Journal of Biotechnology, Indian Journal of
Experimental Biology and Journal of Plant Biochemistry and Biotechnology. She
has one patent to her credit. Currently she is also the coordinator of NET coaching
classes in the Department of Biotechnology. She has twenty years of research and
sixteen years of teaching experience.
DR. HUMAIRA FAROOQI

Specialization : Protein Biochemistry and Stress Biology
Email : hfarooqi@jamiahamdard.ac.in, hfar10@gmail.com
Mobile : 9811483436
Contact No. : 011-26059688 (Extn : 5585)
Dr. Humaira Farooqi did her Ph.D. in Biochemistry from Department of

Biochemistry, Jamia Hamdard. She joined Department of Biotechnology, Jamia
Hamdard in 2008 and is currently serving as an Assistant Professor in the
University. Dr. Humaira holds specialization in protein biochemistry and stress
biology. Her research interests are mainly focused on three major areas which
includes protein folding: protein stability determination/mechanism of protein
stabilization, stress biology and bio efficacy of natural products and their role in
cancer chemoprevention.
Dr. Humaira Farooqi has contributed progressively in field of protein stress
biology and oxidative stress. Her accomplished research works includes
investigation of the conformational changes in proteins exposed to different stress
conditions in vitro and evaluating its mechanism using circular dichroism and in-
silico studies, establishing protective and ameliorative effects of natural
compounds such as GPLC and Genistein against induced oxidative stress. The
outcome of such studies has contributed significantly in oxidative stress research.
Currently her lab is into the investigation of therapeutic potential of active
phytoconstituents from traditional medicinal plants with reference to their
anticancer properties. Dr. Humaira has published 30 research articles in
National and International journals. She has successfully guided more than 50
post graduate students and has supervised 9 Ph.D. students. She has also served
convener and co-convener in national seminars.
DR. JAGRITI NARANG

Specialization : Nano-enabled Interfaces for Diagnostic Applications
Email : jagritinarang@jamiahamdard.ac.in, jags_biotech@yahoo.co.in
Mobile : 9315361479
Contact No. : 011-26059688 (Extn : 5504)
Dr. Jagriti Narang is Assistant Professor at Department of Biotechnology, Jamia

Hamdard, New Delhi and has work experience of more than 8 years. She has authored
or coauthored many research papers in international peer-reviewed journals. She hold
patents on biosensors and uses of nanotechnology in molecular techniques. She has
edited 3 books and written several chapters. She was awarded a silver medal for
extraordinary contribution during her PhD. She has also been project investigator and
co-project investigator in two projects by the Department of Science and Technology
(DST), Government of India. She is committee member of many international
conferences and is member of Material Research Society of India and Society of
Biological Chemistry. She is also editor and peer reviewer of many international
journals and has presented her papers on several international platforms.
Main focused research area is development of nano-enabled interfaces/ nano-
biosensors for the detection of various diseases such as dengue, Chikungunya, cancer,
pathogenic viruses etc. and clinical metabolites. Paper based platforms are exploited
for the multiplexed detection of various metabolites. As a team extensive work was
done on the fabrication of various biosensors for the diagnostic applications. She has
proposed some laboratory models that can be converted into commercial monitoring
devices.
DR. JAVAID AHMAD SHEIKH

Specialization : Immunology
Email : jasheikh@jamiahamdard.ac.in
Mobile : 9417666314
Contact No. : 011-26059688 (Extn : 5581)
Dr. Javaid Ahmad Sheikh pursued doctoral studies from PGIMER, the top most
medical college of our country in the field of Peptide based vaccine development
against tuberculosis. I successfully emphasized the potential use of comparative
genomics and reverse vaccinology to predict the promiscuous vaccine candidates.
Apart from his challenging work, I undertook other projects too and deciphered
therapeutic efficacy of rAg85B and various other DNA based vaccines (J Immune
Based Ther Vaccines. 2011 Jun 26;9:40). My first stint with clinical translational
study was a collaborative study to distinguish Sarcoidosis from TB at molecular
level (J Infect. 2010 Jun;60(6):501-3). Thereafter, other translational works
included deciphering the serodiagnostic potential of some RD based peptides
(Diagn Microbiol Infect Dis. 2014 Apr;78(4):391-7) along with differentiating levels
of Immune complexes in TB and Sarcoidosis (Indian J Med Microbiol.
2017;35(2):290-292).
During my post-doctoral studies and there onwards, here at Jamia Hamdard, I
have been working in basic sciences and translational research regarding various
aspects of TB. It majorly includes the understanding of pathogen virulence and the
host mechanism to fight against the disease with translational aspect of discovery
of new diagnostics and drug repurposing (Front Microbiol. 2016;7:719). I am
currently exploring the moonlighting functions of mycobacterial proteome and how
it exploits host signalling to develop successful intracellular infection (FEBS Open
Bio. 2020; 10(1):70-85). My interests also include uncovering host mediated
defences that involves in-depth exploration of host innate and adaptive immune
responses against pathogens (Lancet Infectious Diseases. 2020, 20(3), 272-273).
Much recently, I have been involved in a collaborative programme to work on
current pandemic of COVID-19. I have been exploring the phylogenetics of global
SARS-CoV-2 strains to ascertain their evolution and relation with disease
dynamics (Infect Genet Evol. 2020 Apr 23:104330; Indian J Med Res. 2020
May;151(5):474-478).
Dr. MAIRAJ AHMED ANSARI

Specialization Host Pathogen Interaction and Molecular Immunology
Email : drmansari@jamiahamdard.ac.in, mairaj01@gmail.com
Mobile : 7455843561
Contact No. : 011-26059688 (Extn : 5589)
Dr. Mairaj Ahmed Ansari, completed Ph.D. in Biotechnology (molecular-immunology

and vaccinology) from Interdisciplinary-Biotechnology-Unit, AMU, Aligarh in the year
2011. He, has more than five years of post-doctoral research experience from Rosalind
Franklin University of Medicine and Science, Chicago, USA. After returning India as
Ramalingaswami-Fellow (DBT, Govt. of India) he joined ICMR-National-JALMA-
institute for Leprosy and OMD, Agra. Currently, he is working as an Assistant
Professor in the Department. His laboratory holds DBT/Wellcome Trust India Alliance
Intermediate-Fellowship (International grant). His laboratory is involved in the host
pathogen interaction research, which includes, developing exosome as tool; for vaccine
candidate, diagnostics and chemotherapy, innate immune responses and their
subversion mechanisms by intracellular pathogens including Mycobacterium
tuberculosis and Herpesviruses. His group is keen to decipher mechanism behind
nuclear sensing of pathogenic DNA and downstream instigation of host innate-immune
response like inflammasome and Type-I-IFN responses. From Ramalingaswami-
Fellowship funding, he has established, well-equipped BSL-II-B2 cell-culture-
laboratory. Importantly, his laboratory is well funded from, DBT, DST-SERB, Wellcome
Trust and ICMR.
He has qualified, UGC-CSIR-NET, ICMR-JRF, GATE and ICMR-PDF awards. Moreover,
he has received two international travel grants. He is life member of Indian-Virology-
Society, Indian Society of Immunology, American society of Microbiology and other
scientific agencies. Dr. Ansari has published 22 research articles in international
Journals and four book chapters. He is regular reviewer in various International
scientific journals. At present, he is mentoring two Ph.D. students.
He has actively participated in twenty national and international conferences to
present his findings either as oral or poster presentation. Actively participated in
organizing National Seminar entitled “Biotechnology research in India: Current status
and future prospects” from 26th and 27th March, 2019 as Co-convener, also served
as Post-Doctoral-Research-Conclave-2019 organizing committee member. His lab is
open for enthusiastic and dedicated research scholars and also for scientific research
collaborations with other labs across the globe.
PROFESSOR MALIK ZAINUL ABDIN
Specialization : Plant Biotechnology and Physiology
Email : mzabdin@jamiahamdard.ac.in
Mobile : 9818462060
Contact No. : 011-26059688 (Extn : 5580)
Prof. Malik Zainul Abdin is Professor in the Department of Biotechnology, School of

Chemical and Life Sciences, Jamia Hamdard, New Delhi. He is a Life member of
National Academy of Science, India. He has also served as Vice-Chancellor, Singhania
University, Rajasthan in 2008 and also as Finance Officer of Jamia Hamdard in the
year 2017-18. He has received his Ph.D. in Plant Physiology. Professor Abdin has 35
years of Research Experience and 30 years of post-graduate Teaching Experience in
the subjects of Botany and Biotechnology. He has taught Molecular Genetics and
Functional Botany to Post graduate students from 1995-1998 in the Department of
Botany and teaching Molecular Plant Physiology, Plant Biotechnology and
Environmental Biotechnology to Post Graduate students in the Department of
Biotechnology from 1998. He has 210 research publications maximum impact factor
of 11.7 and more than 2257 citation index to his credit. He is the Life member of
various professional societies, Coordinator and chairperson of various committees,
members of various administrative and academic bodies. He has given numerous
invited talks, Lectures and tutorials in various National and International Conferences
and Seminars. Professor Abdin has organized more than 24 National Seminars,
Workshops etc. He has completed more than 24 R&D projects costing more than 5
Crores from various funding agencies and guided more than 80 Ph.D. scholars. He
has number of Book Chapters and proceedings in various International and National
Conferences. He has also edited 5 books published by Springer and Elsevier.
PROFESSOR PRADIP KUMAR CHAKRABORTI, HoD

Specialization : Molecular Biology
Email : pkchakraborti@jamiahamdard.ac.in, pradipkchakraborti@gmail.com
Mobile : 9815863975
Contact No. : 011-26059688 (Extn : 5583)
Professor (Dr.) Pradip K Chakraborti received his Ph.D. in the area of biological
sciences from Visva-Bharati (a Central University founded by Nobel laureate
Rabindranath Tagore), Santiniketan, India. He pursued his postdoctoral fellowship in
Canada and USA. On completion of his training in Molecular Biology/Biochemistry
under the Fogarty International program from the National Institutes of Health,
Bethesda, USA, Prof. Chakraborti joined the CSIR-Institute of Microbial Technology,
Chandigarh as a Senior Scientist in 1993. His work on the biology of Mycobacterium
tuberculosis involves notable contributions in understanding the involvement of a
natural transport system in the process of efflux mediated drug resistance,
characterization of a eukaryotic-type serine/threonine kinase that has a role in
mycobacterial cell division as well as peptidoglycan synthesis, and establishing peptide
deformylase as an essential enzyme. These works hold great promise in understanding
their fundamental mechanistic contributions as drug targets for screening/designing of
novel antimycobacterial compounds. His studies resulted in several publications in
reputed peer reviewed journals, patents and book chapters (see Research gate, Google
scholar etc.). Prof. Chakraborti is a recipient of the National Bioscience Award for
Career Development and the prestigious JC Bose National Fellowship. He is an elected
fellow of all three prominent Science Academies in India, the National Academy of
Sciences, Indian Academy of Sciences and the Indian National Science Academy. He is
an elected member of Guha Research Conference, a regular member of American
Society for Biochemistry and Molecular Biology, a Life member of Society for Biological
Chemists. He joined Jamia Hamdard as a Professor in the Department of
Biotechnology at School of Chemical and Life Sciences in 2017. Prof. Chakraborti has
guided many graduate (M.Sc/Ph.D) students over the years and is involved in different
administrative activities within or outside Jamia Hamdard.
(see https://en.wikipedia.org/wiki/Pradip_K._Chakraborti#Selected_bibliography).
PROFESSOR PRATIMA RAY

Specialization : Molecular Virology, Vaccinology
Email : pray@jamiahamdard.ac.in, pratimaray.aiims@gmail.com
Mobile : 9811712425
Contact No. : 011-26059688 (Extn : 5582)
Professor Pratima Ray teaches at the Department of Biotechnology, Biochemistry and

Toxicology at Jamia Hamdard University. Her current research interests include
vaccines and diagnostics against emerging viral diseases in humans. She has recently
established “Indo-US Program on Human Immunity to Chikungunya Viruses” (Indo-US
VAP, DBT) at the international level funded by the Department of Biotechnology and
“National Hospital Based Rotavirus Surveillance Network” funded by ICMR. Her
laboratory primarily focuses on “Molecular Virology and “Vaccinology”. She has guided
several PHD students, post doctoral fellows, doctoral students and masters student.
Her recent work at All India Institute of Medical Sciences(AIIMS) and Jamia Hamdard
University has been published in several peer-reviewed high impact journals like J of
infectious Diseases, Vaccine, J of Microbiology, J Clinical Virology, Pediatric Infectious
Disease and her work was highlighted in the 2008 issue of the journal Nature.
Professor Ray has been involved in vaccine development for over three decades. During
this period she has worked at premiere institutes in India (AIIMS, ICGEB etc) and the
US, developing potential candidates for Malaria, Rotavirus and Chikungunya vaccines.
Of these, the rotavirus vaccine development has come to fruition most recently which
is now included in the country’s Universal Immunization Programme ( July 2015). For
her efforts, the Department of Biotechnology, Govt. of India honored Professor Ray with
a Performance Excellency Award in 2013. Professor Ray began her career as a
Research Scientist at the International Centre for Genetic Engineering and
Biotechnology (ICGEB) before joining at the Department of Pediatrics at AIIMS. She is
an elected life member of the American Society of Virology, Indian Immunology Society
and the Indian Virology Society. Prof Ray received her doctoral degree in
Immunology/Molecular Biology from the University of Delhi. She also holds a Masters
in Philosophy from Jawaharlal Nehru University. During has worked as visiting faculty
in Stanford University, Emory University and Center for Disease Control, USA.
DR. SAIMA WAJID

Specialization : Clinical Genomics and Proteomics, Molecular Oncology
Email : swajid@jamiahamdard.ac.in, wajid.saima@gmail.com
Mobile : 9899823898
Contact No. : 011-26059688 (Extn : 5584)
Dr. Saima Wajid joined Department of Biotechnology, SCLS, Jamia Hamdard

as Assistant Professor on December 07, 2010.
She did Ph.D. in Cancer Biology from National Institute of Cancer Prevention
and Research, ICMR & AMU in 2006. She did her postdoctoral research as a
DST Fast Track Young Scientist at the National Institute of Immunology, New
Delhi from 2007-2010. She was awarded Gold Medals for First Class First
position in M.Sc. and B.Ed. She is elected as Member, National Academy of
Medical Sciences (India) in 2019.
Dr. Wajid is actively involved in research and has mentored 20 Ph.D. students
(8 as Supervisor and 12 as Co-Supervisor) & 1 Post-Doc in the area of
Biotechnology. Presently 5 Ph.D. students are enrolled under her supervision.
She has published 59 original research publications and numerous
abstracts in peer reviewed journals of high impact (Available on ResearchGate
& Google Scholar). She has supervised more than 60 M.Sc. dissertations. She
successfully completed a major research project from ICMR (23.03.2015 to
23.05.2019). She has also participated in several conferences and workshops
as resource person.
Dr. Wajid's research work is related to human health care system putting in
valuable contributions to National/International issues. Dr. Wajid on joining
Jamia Hamdard concentrated in different research projects focusing on
identification and characterization of possible biomarkers in prostate, breast
and oral cancer, molecular investigation of effects of PCOS and
endometriosis/uterine leiomyomas on human uterus, differential protein
expression in renal amyloidosis and evaluation of therapeutic potential of
herbal based nano-formulation on breast cancer cell lines.
Dr. Wajid teaches various course papers at Doctoral, Post-Graduate and
Graduate level and participates as Member of various committees on campus.
As Convener, she coordinated a National Seminar on “Biotechnology in
Healthcare: Challenges and Opportunities”, March 18th & 19th, 2017,
organized by Department of Biotechnology, SCLS, Jamia Hamdard.
ADMINISTRATIVE AND TECHNICAL STAFF
S. No. Name Designation
1 Mr. Ajmal Abbasi Sr. Assistant
2 Mr. Anish Cherian Assistant
3 Ms. Noor Fatima Technical Assistant
4 Mr. Syed Naved Quadri Research Assistant
5 Mr. Mubarak Khan Lab Assistant
6 Mr. S. Zafar Nazish Lab Assistant
7 Mr. Diwan Mohammad Lab Attendant
8 Mr. Satish Chand Lab Attendant
9 Mr. Sharfuddin Lab Attendant
10 Mr. Hemraj Mali
OUTSOURCING STAFF
S.No Name Designation
1 Mr. Wajid Ali Technical Assistant
2 Mr. Islamuddin Office cum Lab Attendant
3 Mr. Murad Bux Lab Attendant

STUDENT SUPPORT FACILITIES
CENTRAL INSTRUMENTATION FACILITY (CIF)
The school has a well-equipped Central Instrumentation Facility which is open to all
the students round the clock. It is equipped with state-of-art equipments. These
include Real Time PCR, Nanodrop spectrophotometer, Confocal Microscope, Atomic
absorption and Atomic emission spectrophotometers, inductively coupled plasma
spectrophotometer, ELISA reader, JASCO Spectro-polarimeter, Ultracentrifuges, HPLC
with integrator, Gas chromatograph, HPTLC, CHNS Analyser, Gamma and Beta
Scintillation Counters, Ultrascan, FT-IR, Luminescence Spectrometer, UV-VIS Double
Beam Spectrophotometer, Gel Documentation System and Semi-preparative HPLC.
Facilities for Internet and DTP are also available. All the Departments of the faculty are
connected to the server of the CIF.
HAKIM MOHAMMED SAID LIBRARY
The University library is among the oldest academic institutions established by the
great Visionary late Chancellor Hakeem Abdul Hameed Sb. (1908-1999) in 1977 at the
Tughlakabad Campus of the upcoming university. The Central library is housed in a
multi-tirer round building famous for its architectural design and beautiful
landscaping. It was renamed in 1992 as the Hakeem Mohammed Said Central library
(acronym HMSCL) on the name of the younger brother of the founder. The Jamia
Hamdard Library System consists of the HMS Central library and a number of School
libraries, such as School of Pharmaceutical education and Research Library, School of
Nursing Sciences and Allied Health Library, School of Unani Medical Education and
Research Library, School of Chemical and Life Sciences Library, School of Humanities
and Social Sciences Library, HIMSR Library and HILSR Library etc. holding good
number of Books and Periodicals.
The library system is well equipped with latest different Subject Books, Text Books,
Reference Books, Periodicals, Magazines, Databases, online electronic sources, etc.
concerning the various courses taught in the university. The collection is distinguished
for documents on bio-medical sciences, Medical sciences, Biotechnology, Food
Technology, Pharmacy, Management, Information Technology, Computer Sciene,
Islamic studies, medieval history and the traditional system of medicine (unani).‘The
six voyages of John Baptista Travernier’, published in the year 1678 is the oldest
printed book available in the library collection. The manuscript section besides 164 a
number of rare documents has the distinction of holding the only extant copy of al-
Mudkhal ila Ahkam al-Nujoom (a handwritten book on Astrology in Arabic language)
dated 3rd century of Hijri calendar (equivalent to 8th century AD). The salient features
of the collection at a glance:
 Over 2 lacs books.

 Subscribing 83 current scholarly journals
 Subscribing Online Four databases.
 3494 Manuscripts, 3300 Bound volumes of journals.
 83 Rare Books Digitized (In House).
Additionally, the following unpublished, locally created outputs generated by the
research scholars and faculty members of this university are made available:
 650 doctoral theses, 2500 Post-graduate dissertations.
 University publications.
Library services
Various conventional & non-conventional services are provided to the students and
scholars from 9.00 am to 9.00 pm all weekdays, timings are periodically extended
during Examinations.
 Charging/Discharging of documents.
 Book Bank service.
 Inter-library loan service (through DELNET).
 Documents delivery service (DDS).
 Current awareness service (CAS)
 Electronic theses & dissertation (ETD) service.
 Short-range and long-range reference service.
 Anti-plagiarism check (through web tools Urkund).
 Member of National Digital Library.
 Demonstration of commercial databases for procurement.
HERBAL GARDEN
Jamia Hamdard has the privilege of maintaining a herbal garden within the campus
which has about 150 species of important traditional medicinal and aromatic plants.
The main purpose of the garden is to carry out experimental work and initiate the ex-
situ conservation of rare medicinal plants. It also helps in various research projects by
providing raw material.
COMPUTER CENTRE
The University has excellent state-of-the-art computing facilities and system analysis
units in the computer centre to cater the needs of students. The computer centre is
well equipped with advanced computers along with all the necessary peripherals as
well as requisite softwares. Students also get unlimited and uninterrupted Internet
facility by University Wi-Fi facility.
FACULTY LEVEL ANTI RAGGING SQUAD
Ragging in any form is strictly and legally prohibited in JAMIA HAMDARD. Anyone
found guilty will be dealt in accordance with the rules and regulations of JAMIA
HAMDARD. Punishment could include fine, suspension and/or expulsion from the
University. Any student (Fresher/ others) subjected to ragging shall report immediately
WOMEN CELL
The Women’s Cell of Jamia Hamdard was established in 2015 on the issue of sexual
harassment in the work place. Women’s Cell was formed to empower and uphold the
dignity of Women at work. The University aims to provide and maintain a dignified,
safe, congenial working and learning environment for women employees and students
free from gender discrimination and sexual harassment. It further cultivates the
atmosphere where men and women work together towards the growth and prosperity
of the institution in a safe and healthy academic environment.
The Women Cell is headed by a senior female faculty member and its main functions
are:
 To look after all activities and issues related to women at Jamia Hamdard.
 To organize activities/seminars/etc. for sensitizing faculty and students about
gender issues.
 To look into all the grievances of women students/employees of Jamia Hamdard
EQUAL OPPORTUNITY CELL
The main objective of the Equal Opportunity Cell of Jamia Hamdard is to help and
empower the persons with disabilities, students to participate fully in the academic,
intellectual, social and cultural life of University on an equal basis.
Functions:
a) To ensure equity and equal opportunity to the community at large in the college
and bring about social inclusion.
b) To enhance the diversity among the students, teaching and non-teaching staff
population and at the same time eliminate the perception of discrimination.
c) To create a socially congenial atmosphere for academic interaction and for the
growth of healthy interpersonal relationships among the students coming from
various social backgrounds.
d) To make efforts to sensitize the academic community regarding the problems
associated with social exclusion as well as aspirations of the marginalized
communities.
e) To help individuals or a group of students belonging to the disadvantaged
section of society to contain the problems related to discrimination.
f) To look into the grievances of the weaker section of society and suggest amicable
solution to their problems.
g) To establish coordination with the Government and other
agencies/organizations to mobilize academic and financial resources to provide
assistance to students of the disadvantaged groups.
h) To organize periodic meetings to monitor the progress of different schemes.
i) To adopt measures to ensure due share of utilization by SC/ST in admissions,
recruitments (teaching and non-teaching posts) and to improve their
performances.
j) To sensitize the college on the problems of SC/ST and other disadvantaged
groups.
UNIVERSITY WEBSITE
www.jamiahamdard.edu and www.jamiahamdard.ac.in are websites for resources of

information about the University. Starting with the announcement of the admission,
list of short-listed candidates, regular notices are uploaded on the web site time to
time.
HEALTH CARE
Students of Jamia Hamdard are eligible for free consultation in allopathic and Unani
OPDs. Emergency services to students are also provided free of cost. Cost of medicine
has to be met by the students.
HAMDARD CONVENTION CENTRE
Beautifully designed and centrally air-conditioned Convention Centre has attracted

scholars, researchers and organizations from all over the world. It is elegantly
furnished and provided with all the modern amenities for organizing seminars,
conferences, meetings and lectures.
HOSTELS
University has following hostels for boys and girls:
 Anne Marie Schimmel Hostel - PG girls

 Ibn-e-Sina Hostel – UG and PG girls
 Abdul Mueed Hall of Residence boys Hostel
 Ibne Sina ANNEXE /Nursing Hostel
A new state of the art Mess and a fully air-conditioned girls' hostel, with a capacity 400
beds, is underway and shall be ready in the third quarter of 2020.
SPORTS AND CULTURAL ACTIVITIES
Jamia Hamdard has Indoor and Outdoor sports facilities in the campus. For Indoor
facilities, a hall consisting of well-equipped physical fitness centre/gym, two
badminton courts, table tennis, carrom board and chess etc. are available with day
and night facility. For outdoor facilities, Jamia Hamdard has athletic track, well
maintained cricket ground, football ground and basketball & volleyball courts with
proper LED lights for day and night matches. Jamia Hamdard also organizes inter-
school sports tournaments (Hamdard Sports Meet) and Literary & Cultural Events
(Hamdard Fest) annually. Various inter and intra school tournaments are organized by
the university throughout the year. Various teams of Jamia Hamdard also participate
in sports & cultural activities in National, Regional and Zonal level events. Jamia
Hamdard has organized Zonal and National tournaments in past. The boys and girls
team of basketball and volley ball participated in inter zonal tournaments organized by
Association of Indian Universities. The cricket and football teams have also
participated in inter-university tournaments organized by Association of Indian
Universities.
NATIONAL SERVICE SCHEME (NSS)
National Service Scheme (NSS) is a program sponsored by the Ministry of Youth Affairs
and Sports, Government of India. The NSS volunteers participate in various awareness
program such as Pulse Polio Immunization camps, awareness about Hepatitis-B, DPT
and BCG immunization, HIV/AIDS, STD, importance of cleanliness, illiteracy
eradication program, plantation and related activities. NSS volunteers also participate
in program sponsored by Delhi AIDS Control Society. NSS volunteers organize blood
donation camp every year. A 15-day Special camp is organized by NSS volunteers
every year in slums of Delhi for community awareness. NSS Jamia Hamdard is a
member of the Red Ribbon Club which is HIV/AIDS awareness scheme sponsored by
Government of NCT, Delhi. The number of NSS volunteers in Jamia Hamdard is
around two hundred.
NATIONAL CADET CORPS (NCC)
NCC is a highly reputed body and has an enormous history to take pride. In fact, NCC
is always ready for the nation by serving the best of the abilities and capabilities to the
people, for the people and by the people. As NCC is not just meant for military training
but it has also enabled the cadets to learn several other valuable things for life such as
selflessness, discipline, hard work, honesty and other leadership qualities. We have
54 cadets, in which most of the cadets have completed B or C certification and
attended various CATC, Trekking National Camp, Army Attachment and Others
National Camps. NCC Jamia Hamdard also organized an Annual Conference on 30th
October 2019 on “Contribution of NCC Cadets for Nation Building and Global
Development”. Our cadets actively participate in Blood Donation camps, Jal Shakti
Abhiyan and Swachta Pakhwada Mission of Government of India.
STUDENTS AID FUND
The University has allocated some amount as Students Aid Fund which is meant for
financial help to the needy students. A student, whose parents/guardians have income
of less than Rs.10,000/- per month along with his/her performance at examinations
conducted by Jamia Hamdard is eligible for applying for the assistance from this fund.
PLACEMENT CELL
To facilitate the placement and summer training of M.Sc. Biotechnology passing-out

and passed-out batches in R&D institutions, Universities and Industries a Placement
Cell was established by Prof. M.Z. Abdin in Department of Biotechnology in 2014. The
coordinators of the cell are Dr. Humaira Farooqi and Dr. Saima Wajid. The placement
cell has helped our students in fetching the job offers in various companies including
AceProbe Technologies (I) Pvt. Ltd, Double Helix Clinical Cytogenetics & Reproductive
Immunology Centre and Genetix Biotech Asia Ltd.
MAJOR ACHIEVEMENTS OF OUTGOING STUDENTS

The students who successfully graduated from this department have been selected in
various institutions including DRDO, CSIR laboratories, universities (Delhi University,
Guru Gobind Singh University, Kashmir University, etc.), medical colleges such as
PGIMER (Chandigarh), CSIR-IMTECH (Chandigarh) and industry. Many opt for the
PhD, and have been taken by premier institutes and research laboratories such as
CSIR-CCMB (Hyderabad), IISc (Bangalore), ICGEB (New Delhi), AIIMS (New Delhi), NII
(New Delhi), Dabur Research Foundation (Ghaziabad), NICD (New Delhi), NCBS (New
Delhi), CSIR-CDRI (Lucknow), TERI (New Delhi), IARI (New Delhi), JNU (New Delhi),
DU, South Campus (New Delhi), CSIR-IGIB (New Delhi), CDFD (Hyderabad) and
laboratories abroad in USA, Germany, Denmark, Canada etc.
NET COACHING CLASSES
Department organizes NET coaching classes for the M.Sc. Biotechnology students
under the guidance of Head, Dept. of Biotechnology. The Coordinators are Dr. Alka
Narula, Dr. Humaira Farooqi, Dr. Jagriti Narang, Dr. Javaid Ahmad Shaikh and Dr.
Mairaj Ahmed Ansari. The NET coaching classes are held on Saturdays from 9.00 AM
to 1.00 PM. These classes are taken by the teachers from the Dept. of Biotechnology
and also from other departments of School of Chemical and Life Sciences as well as
outside experts. M.Sc. students are asked to submit those topics where they need
coaching and to be discussed during NET coaching classes.
MONTHLY DEPARTMENTAL SEMINAR
Monthly departmental seminar is being organized in the department from experts of

the field to keep our students updated with ongoing cutting-edge scientific research.
SCHOLARSHIPS & FELLOWSHIPS
The following scholarships / fellowships are available for the students.

Eligibility
General for all Schools
a) Hamdard National Foundation (HNF) Scholarship For limited PhD. students on

the merit-cum-means basis.
b) Khwaja Moinuddin Chishti Ajmeri (Gharib Nawaz) merit-cum-means scholarship
to be awarded on the basis of essay writing competition on the life and work of
Khwaja Gharib Nawaz.
c) Dr. L.M. Singhvi Gold Medal (or Silver Shield) of accolade for the first and
second winner of essay competition on a subject of topical importance of
interfaith dialogue conducted every year.
d) Hakeem Abdul Majeed Scholarship (for Pharmacy, Medicine (U), Nursing and
Science Faculties) for the students who have secured highest marks in annual
exams of B.Pharm. or B.Pharm. (Unani) I, II and III Years (Under regular
scheme), BUMS I & II Prof., B.Sc. Nursing, I, II and III Years and M.Sc. I Year.
School of Chemical and Life Sciences
a) Mrs. Shakila Naqvi Merit Scholarship for a (Muslim) female student of M.Sc. II
year who secures highest marks in M.Sc. I Year of Faculty of Science.
b) Tasmia Merit Scholarship for a student of M.Sc. II year Biotechnology on the
basis of merit of the candidate.
c) Noorul Hasan Memorial Scholarship for a student of II year of M.Sc.
Biotechnology on the basis of merit and need.
d) Prof. A.K.M. Ghouse Means-cum-Merit Scholarship for a student of M.Sc. II Year
Botany (Environmental Botany), on the basis of merit.
e) Anchrom Means-cum-Merit Scholarship for a student of M.Sc. II Year Chemistry
(Industrial Applications) on the basis of merit of the candidate.
f) Dr. Manoj Varshney Scholarship for a student of M.Sc. II Year Chemistry
(Industrial Applications) on the basis of merit.
g) Khalil Ahmad Merit-cum-means Scholarship for a student of M.Sc. Chemistry
on the basis of merit and need.
h) Late C.R. Arora Scholarship for a meritorious and needy student of M.Sc.
Chemistry (Industrial Chemistry) Final year.
i) Hind Agro Industries Merit Scholarship for a student of M.Sc. II year Toxicology
on the basis of merit-cum-financial need of the candidate.
j) Tasneema Fellowship for a female student of M.Sc. II Year (Toxicology) for
studies on Regulatory Toxicology
k) Late Hajji Mohammad Asif (s/o late Janab Mohammad Ishaque Sandook Wale,
1319, Pahari Imli, Delhi-6) Reward for knowledgeable student (MARKS)
Scholarship for bonafide Muslim student / students of MSc (either in
Biochemistry, Biotech, Chemistry-Industrial Applications, Environmental
Botany or Toxicology) for II year.
ALUMNI ASSOCIATION
Department has established an Alumni Association. Passing-out and passed-out
batches have to submit the prescribed application form in the Office of the
Biotechnology for registration in the Association. A webpage has already been created
for Alumni Association. The coordinators of Alumni Association are Dr. Humaira
Farooqi and Dr. Saima Wajid.
The Alumni Association of Jamia Hamdard (TAAJH)

The Alumni Association is registered as, “The Alumni Association of Jamia Hamdard”
(TAAJH) under Societies Registration Act XXI of 1860 in Distt. South-East,
Government of NCT of Delhi. The TAAJH is located at Jamia Hamdard G/F, M.B.
Road, Hamdard Nagar, New Delhi-110062. The Registration Number of TAAJH is – S-
E/1462/Distt. South East/2018. The mission of the alumni is to consolidate the
alumni base of Jamia Hamdard on official platform endorsed by the university. This
would give the feeling of “belongingness” to the alumni and they would “feel connected”
and would continue to take pride in our achievements and would do handholding
where ever required. The platform would be constructively used with all good
intentions to serve the ALMA MATER & HAMDARDIANS and is expected to fulfils the
objectives. The aims and objectives of the alumni are given below:
a) To act as a bridge between Jamia Hamdard and the industries/ organizations

where the alumni (HAMDARDIANS) serve, for interaction on new developments
in different disciplines of Health Sciences (Unani, Medical, Pharmacy and
Nursing), Allied health fields (Physiotherapy/ Rehabilitation Sciences), Life
Sciences, Information, Technology and Communication (IT) and Management
and others.
b) To provide a constructive, vivacious and vibrant ecosystem for fostering ties

between the alumni and the alma mater and as well as amongst the alumni
across the schools.
c) To offer support to desirous students of different Schools for carrying forward

their education in India and abroad.
BOOKS FOR REFERENCES

S.No. Author Title Edition
1 Brown Biochemistry 1st
2 Daugherty.E The new millennium 1st
3 Pierce B.A. Genetic A Conceptual Biotechnology science for Approch 6th
4 Brown T.A. Genomics 4 1st
5 Marshall.CE Microbiology a Textbook of Microorganisms General & Applied 1st
6 Parker Microbiology 1st
7 Glick B.R. Mol. Biotechnology principles & App. of recombinant DNA 5th
8 Lodish Berk Molecuar Cell Biology 8th
9 Brown Biochemistry 1st
10 Daugherty.E The new millennium 1st
11 Pierce B.A. Genetic A Conceptual Biotechnology science for Approch 6th
12 Brown T.A. Genomics 4 1st
13 Marshall.CE Microbiology a Textbook of Microorganisms General & Applied 1st
14 Viswanath Buddolla Environmental Biotechnology 1st
15 Sangdun Choi Introduction to Systems Biology 1st
16 P.K. Mohapatra Textbook of Environmental Microbiology 1st
17 G.Tripathi Cellular and Biochemical Sciences 2nd
18 Alexander N.G. Microbial Biotechnology 2nd
19 Viswanath Buddolla Environmental Biotechnology 1st
20 Michael J.H. Ratcliffe Encyclopedia of Immunbiology Volume-2
21 Pal Maliga Chloroplast Biotechnology 1st
22 Ronald Ross R.Preedy Genetically modified organisms in food 3rd
23 James D. watson Recombinant DNA 3rd
24 Campbell Biology-A Global Approch 10th
25 Michael Fry Landmark experiments in Molecular biology 1st
27 Pal Maliga Chloroplast Biotechnology 1st
28 Ronald Ross wastson Genetically modified organisms in food 3rd
29 James D. watson Recombinant DNA 3rd
30 Campbell Biology-A Global Approch 10th
31 Michael Fry Landmark experiments in Molecular biology 1st
33 Jon Lorah Methods in Enzymology 1st
34 Gautam B. Singh Fundamentals of Bioinformatics and computational Biology 1st
35 Robert N. Trigiano Plant Development and Biotechnology 2nd
36 Oksman Plant Biotechnology and Transgenic Plants 3rd
37 Ramesh C. Ray Fermented Foods 1st
38 Frank H. Stepenson Calculation for Molecular Biology & Biotechnology 3rd
39 David B. Collinge Plant Pathogen Resistance Biotechnology 1st
40 Carrie A. Eckert Biotechnology Biofuel production and Optimization 1st
41 Daniel A. Vallero Environmental Biotechnology 2nd
42 Alberts Mol. Biology of The Cell 6th
43 Lincoin Taiz Plant Physiology and Development 6th
44 Jeffrey C. Pommerville Alcamo’s Fundamentals of Microbiology 10th
45 Benjamin S.Weeks Alcamos Microbes & Society 3rd
46 Edward A.Greenfield Antibopdies A Laboratory Manual 2nd
47 David A. Micklos Genome Science 1st
48 Rafael Yuste Imaging 1st
49 Lynne Cassimeris Lewin’s Cell 2nd
50 Jocelyn E. Krebs Genes XI 11th
51 Slonczewsk Foster Microbiology an Evolving Science 2nd
52 Burton E. Tropp Molecular Biology Genes to Proteins 4th
53 Florence K. Gleason Plant Biochemistry 1st
54 Burton E. Tropp Principles of Molecular Biology 1st
CHOICE BASED CREDIT SYSTEM (CBCS)
SEMESTER – I
Course Code Course Name Paper Category Duration Credits Max Marks Sessional Exam Marks
MFC 001 Foundation Course Compulsory 120 hrs 8 200 50 150
Course
MBT OE101 Cellular Biology & Biomolecules Open Elective 72 hrs 4 100 25 75
MBT CC102 Molecular Biology Core 72 hrs 4 100 25 75
MBT CC103 Essentials of Genetic Engineering Core 72 hrs 4 100 25 75
MBT CC104 Practical – Biomolecules, Genetic Core 150 hrs 8 200 50 150
Engineering and Molecular Biology
Total 28 700 175 525
SEMESTER – II
MBT CC201 Expression of Genetic Information Core 72 hrs 4 100 25 75
MBT CC202 Molecular Plant Physiology Core 72 hrs 4 100 25 75
MBT CC203 Plant Tissue Culture & its Core 72 hrs 4 100 25 75
Applications
MBT CC204 Biotechnology : Environmental & Core 72 hrs 4 100 25 75

Ethical Aspects
MBT OE205 Biostatistics Open Elective 72 hrs 4 100 25 75
MBT CC206 Practical – Molecular Plant Core 150 hrs 8 200 50 150
Physiology & Plant Tissue Culture
Discipline Centric
MBT DE207 Seminars/ Assignments 36 hrs 2 50 0 50
Elective
Total 30 750 175 575

SEMESTER – III
MBT CC301 Animal Biotechnology Core 72 hrs 4 100 25 75
MBT CC302 Plant Biotechnology Core 72 hrs 4 100 25 75
MBT CC303 Immunology Core 72 hrs 4 100 25 75
MBT CC304 Biotechnology and Human Health Core 72 hrs 4 100 25 75
MBT OE305 Bioinformatics Open Elective 100 hrs 4 100 25 75
Practical: Immunology, Animal Core

MBT CC306 150 hrs 8 200 50 150
and Plant Biotechnology
Discipline Centric
MBT DE307 Seminars/Assignments 100 hrs 2 50 0 50
Elective
Total 30 750 175 575
SEMESTER – IV
MBT CC401 Dissertation/ Viva voce Core 720 hrs 12 300 50 250
Total 12 300 50 250
 One Practical/ Tutorial credit is gained by two hours

 For Open Elective papers candidate has to compensate for the required credits per semester (25) if he/she chooses the elective
paper from any other Department of Science Faculty
 One Open Elective each for first three semesters is proposed, therefore 9 credits may be gained from any other Department of
Science Faculty
FOUNDATION COURSE
MFC 001
Credit :8, Max. Marks: 200 [Sessional Marks: 50, Exam. Marks: 150] Time :120 hours
CHEMISTRY (Unit-I)
1. Solution – Methods of expressing the concentration (Molality, Moarlity,

Normality etc).
2. Laws of mas action, Reaction Quotient, Chemical equilibrium constant,
Relation of Kp & Kc, pH, buffer, buffer index, buffer capacity, Arrhenius
equation.
3. Collagative properties : Molecular mass determination using colligative
properties.
4. Rate of reaction, order of reaction
5. Different types of chemical bonds
6. Principles, classification and applications of chromatographic techniques.
7. Basics of Spectroscopy and applications
8. Basic applications of Nanotechnology.
BIOCHEMISTRY (Unit-II)
1. Biomolecules : Carbohydrates, amino acids/proteins, lipids and nucleotides;

Enzymes: Characteristics and nomenclature.
2. Cell Biology & Microbiology : Prokaryotes & Eukaryotes: The cell and its
composition; Cell organelles and subcellular fractionation; Viruses, Viroids,
Virusoids and Prions; Bacterial culture and growth curve.
3. Bioenergetics and Intermediary Metabolism : ATP as energy currency;
Intermediary metabolism;
4. Immunology - Active, passive, Humoral and Cellular immunity; Clonal selection
theory; Cells of immune system; Immunoglobulins; Haptens, Antigens and
Immunogens; Monoclonal & Polyclonal antibodies.
5. Clinical Biochemistry :Biochemical tests; Acid base disorders; Liver function
test; Kidney function tests.
BOTANY (Unit-III)
1. Biodiversity – Concept, levels and Conservation of biodiversity

2. Climate Change and its consequences
3. Ecosystem - Producers, consumers and decomposers of food chain.
4. Plants with medicinal values
5. Environmental pollution, bioremediation
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BIOTECHNOLOGY (Unit-IV)
1. Genetics of Inheritance - Laws of inheritance, recombination and segregation of

traits, segregation ratio, interaction between traits & quantitative inheritance.
2. Molecular Biology - The genetic material, RNA as genetic material, fidelity of
DNA replication, transcription, translation, gene regulation. Mutation and
mutagenesis, Ames test, Transposons.
3. Genetic Engineering - Essentials of gene manipulation, vectors & enzymes used
in recombinant DNA technology.
4. Biotechnology: Applications and Ethical aspects : Stem cell and its application,
Concept of GM crops and their relevance to society.
TOXICOLOGY (Unit-V)
1. Introduction to Toxicology.
2. Various types of toxicity (Acute, subacute, subchronic and chronic).
3. Chemical interactions (Additive effect, potentiation, synergism and antagonism),
Dose response relationship (ED50, LD50 EC50, LC50.)
4. Routes of exposure, biotransformation of toxicants. In vitro and in vivo models
in toxicological studies.
5. Xenobiotics : Common toxicants of air, water & food and their adverse effect on
health.
DRUG DEVELOPMENT PROCESS (CLINICAL RESEARCH (Unit-VI)
1. Process of drug development

2. Phases of clinical trials
3. Drug regulatory affairs
4. Principles of GLP, GMP & GCP
5. Basic concepts of Intellectual Property rights
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Semester - I
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CELLULAR BIOLOGY & BIOMOLECULES
MBT – OE101
Credit :4, Max. Marks: 100 [Sessional Marks: 25, Exam. Marks: 75] Time: 72 hours
Subject Coordinator : Dr. Humaira Farooqi
UNIT – I
The cell & cell cycle: Structure and function of cell. Phases of the cell cycle; regulation of
the cell cycle by cell growth and extra cellular signals; cell cycle check points; coupling of S-
phase to M phase. Regulators of cell cycles progression – MPF; families of cyclins and cyclin –
dependent kinases; growth factors and D-type cyclins. Inhibitors of cell cycle progression.
Cell growth and division.
Cell Signaling: Signaling molecules and their receptors; functions of cell surface receptors;
pathways of intracellular signal transduction; signal transduction and the cytoskeleton;
signaling in development and differentiation. Bacterial Chemotaxis and Quorum Sensing;
Cell signaling and Cancer.
UNIT –II
Carbohydrates& Lipid Metabolism:

Carbohydrates: Structures and functions of carbohydrates ; Bioenergetics, glycans and
proteoglycans. Aerobic and anaerobic pathways. Glycolysis, TCA cycle, electron transport
chain and oxidative phosphorylation. Galactose and fructose metabolism. Gluconeogenesis,
glycogen synthesis and breakdown.
Lipids: Structure and functions; Metabolism and synthesis of phospholipids, glycolipids,
sphingolipids and other derived lipids. .,  and oxidation of fatty acids. Mobilization of fats.
Inborn errors of metabolism: Glycogen storage diseases in human- Von Gierke’s disease,
Pompe’s disease, Cori’s disease, Mc Ardle’s syndrome. Inherited human diseases related to
membrane lipids -Gangliosidoses, Gaucher’s disease, Niemann-pick disease.
UNIT – III
Amino acid and Protein metabolism : Acid base chemistry of amino acids Amino acid
synthesis and metabolism. Primary, secondary, tertiary and quarternary structure of
proteins. Super secondary structures. Isomerism and types. Dihedral angles, Ramachandran
plot. Hydropathy plot, Models of protein folding. Chaperone assisted protein folding; Amyloid
disease, Dnak and DnaJ mechanism of action; Circular dichroism. Sequencing, Peptide
synthesis, Interrelationship of protein and carbohydrate metabolism. Urea cycle.
Hyperammonemia. Regulation of cell metabolism.
UNIT – IV
Protein purification and enzymes : Cofactors and types; vitamin derived coenzymes (Role of
TPP, CoA, FMN, FAD, PLP, Biotin as cofactors in various enzymatic reactions); Fat and Water
soluble vitamins, and their deficiencies.
Purification of proteins: Salt fractionation, gel filtration (FC), PAGE-native and SDS, ion-
exchange chromatography, affinity chromatography, 2D gel electrophoresis, isoelectric
focusing.
Enzyme Kinetics: Characteristic of enzymes, Nomenclature and Classification,
Michaelis Menten Kinetics Inhibition of enzyme catalyzed reactions, Multifunctional
enzymes, multi-enzyme complexes, coupled reactions, cyclic reactions.
Subject coordinator shall be responsible for completion of course and

submitting attendance as well as Sessional marks to Office of HoD. In case
of any difficulties, students will contact the course coordinator.
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MOLECULAR BIOLOGY
MBT – CC102
Credit :4, Max. Marks: 100 [Sessional Marks:25, Exam. Marks: 75] Time : 72 hours
UNIT – I Subject Coordinator : Prof. Pradip K. Chakraborti
Genetic material: Eukaryotic Genome. Organisation of Chromatin (Supercoiling).

Repetitive DNA, Types of DNA. Properties of DNA in solution. Dissociation-reassociation
kinetics. Cot curves. RNA: RNA genome and its replication. Replication of retroviruses
and reoviruses. Reverse transcriptase; RNA dependant RNA polymerase. Types of RNA
and their primary and secondary structure. Functions of RNA. Replication of DNA. Role
of DNA polymerases and other DNA modifying enzymes. Mechanism of replication.
Multiple origins of replication. Proof reading function and fidelity of DNA replication.
Extrachromosomal replicons. Replication of circular DNA: Theta model and rolling circle
models.: DNA and RNA fingerprinting (including probes and labeling techniques). DNA
sequencing and sequencing strategies: Maxam & Gilbert’s, Sanger’s and Next generation
(454, Solexa, SoLID).
UNIT – II
Transcription : Structure of gene, regulatory and transcriptional units. Promoters and

other regulatory elements. Special features of eukaryotic genes. Exons and introns.
Transcription in prokaryotes. Prokaryotic RNA polymerase and its components.
Initiation, elongation and termination of transcription. Role of Rho factor. Transcription
and translation are coupled in prokaryotes. Polarity effect. Eukaryotic transcription.
Types of eukaryotic RNA polymerases and their role. RNA modifying enzymes.
Transcription factors. Termination of eukaryotic transcription. Modification of primary
transcript in eukaryotes; Removal of introns, Intron-exon junction. Splice sites. Nuclear
splicing, Role of SnRNPs. Self splicing of Type I and Type II introns. Role of ‘G’
nucleotide. Catalytic activity of RNA. Enzymatic splicing of tRNA. Self cleavage of viroids
and virusoids, Ribozyme. Alternate splicing and gene regulation. Cap addition,
polyadenylation and RNA editing.
UNIT – III
Mutation and mutagenesis : Physical and chemical mutagens and their effect on DNA.
Beneficial mutations; Site directed mutagenesis, oligonucleotide directed point
mutations; DNA damage and repair in prokaryotes and eukaryotes. Base and nucleotide
excision mechanisms. Direct repair. Mismatch repair, role of methylation, UV induced
damage and repair system. Error prone repair. SOS response.
Genome Editing : Concept, applications in plants and animals, prospects and
limitations.
UNIT – IV
Molecular Evolution: Separation, natural selection and evolution of proteins as well as,
nucleotide sequences. Molecular clock, evolution by gene duplication and exon shuffling,
deleterious genes. Eugenics, Gene frequencies, conservation of gene frequencies.
Transposable elements in bacteria. Mobile elements in eukaryotes. Insertional sequences
(IS elements), transposons and composite transposons, retroposons. Replicative and non
replicative transpositions. Molecular mechanism of transposition events.

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ESSENTIALS OF GENETIC ENGINEERING
MBT – CC103
Credit: 4, Max. Marks: 100 [Sessional Marks: 25, Exam. Marks: 75] Time: 72 hours
Subject Coordinator : Prof. Pratima Ray

UNIT – I
Mendelian & Post mendelian genetics : Mendelian and Non-Mendelian

inheritance. Inheritance of Quantitative traits. Gene discovery- forward and reverse
genetics approaches. Molecular Mapping and tagging. RNAi, siRNA and miRNA.
Epigenetics. Comparative genomics: insights into genome organization; application
in gene and cis-element detection.
UNIT – II
Genomics of microbes : General characteristics of different groups: acellular

microorganisms (Viruses, Viroids, Prions) and Cellular microorganisms (Prokarya,
Archaea and Bacteria; Eukarya : Algae, Fungi and Protozoa). Mechanism of gene
transfer in bacteria: conjugation, transduction and transformation. Recombination
in Bacteriophages. Mapping the structure of bacterial chromosome. Far western
blotting, Analysis of DNA-Protein Interactions, Electromobility shift assay, Methyl
Interference assay, DNase Footprinting. PCR in molecular diagnostics: Detection of
hepatitis, herpes, HIV, and EBV. The role of PCR in detecting minimum residual
diseases (MRD).
UNIT – III
Characteristics and application of vectors and enzymes: Introduction to gene

manipulation, Plasmid, phages, cosmids, YAC, BAC and Ti-plasmid. Vectors for
making RNA probes. Vectors for maximizing protein synthesis, protein purification
and enhanced protein export. Vectors with combination of features (Litmus and pin
point vector series).
Nucleic acid modifying enzymes (Restriction endonuclease, Polymerase, Kinase,
Phosphatase, Methylase, Ligase). Concept of adapters and linkers for insert
modification.
UNIT – IV
Molecular markers, Cloning and library construction : Construction of genomic

and c-DNA libraries. Synthesis of cDNA (mRNA isolation, purification and
strategies of cDNA synthesis). Cloning strategies (conventional and advanced
cloning strategies). Introducing DNA into bacterial cells. Screening of libraries:
Grunstein Hogness method, probe based screening, Blue and white screening,
replica plating. Southern, Northern, Western and South-Western blotting.
Molecular markers: PCR and derived techniques including RAPD, AFLP, iPCR, qRT-
PCR, RFLP. In-situ hybridization – FISH, GISH. Micro arrays: DNA and RNA
microarrays for transcriptomics and re-sequencing/mutation detection.
Applications of recombinant DNA technology.

16 | P a g e
PRACTICAL – BIOMOLECULES, GENETIC ENGINEERING AND
MOLECULAR BIOLOGY
MBT-CC104
Credit: 8 Max. Marks: 200 [Sessional Marks: 50, Exam. Marks: 150] Time: 200 hours
1. Instrumentation
2. Preparation of solutions and buffers.
3. Water analysis - Hardness, Conductivity and pH.
4. Qualitative and Quantitative analysis of Carbohydrates and
detection of reducing sugars by Folin-Wu-method.
5. Isolation and hydrolysis of starch & casein from biological samples.
6. Quantitative tests of Lipids & their separation by thin layer
chromatography (TLC).
7. Qualitative analysis of proteins and amino acids.
8. Separation and identification of amino acids by ascending paper
chromatography.
9. Extraction of proteins from biological samples and quantification by
Lowry’s and Bradford’s method.
10. Separation of proteins by SDS-PAGE.
11. Subcellular fractionation by differential centrifugation.
12. Sonication of subcellular fractions and quantification of proteins by
micro-Bradford’s assay.
13. Column chromatography (Molecular sieving)
14. Preparation of Luria Bertani (LB) medium for bacterial culture and
solutions.
15. Inoculation of E.coli DH5α strain on LB medium.
16. Extraction, quantification and agarose gel electrophoresis of
genomic DNA from E.coli DH5.
17. Effect of temperature and alkali on UV absorption of DNA:
Hyperchromicity.
18. Preparation of competent cells by CaCl2 method.
19. Ligation of linearized pGEM-T vector with an insert.
20. Transformation of chemically competent E. coli DH5α with plasmid
blue script using CaCl2 and heat shock method.
21. Isolation of plasmid DNA from E.coli by alkaline lysis method.
22. Agarose gel electrophoresis for plasmid DNA.
23. Restriction of λ-DNA, plasmid DNA and bacterial genomic DNA.
24. Agarose gel electrophoresis of restricted samples
25. Southern blotting of gel of restricted samples.
26. Isolation of RNA and its quantitation by UV spectrophotometer.
17 | P a g e
Semester - II
18 | P a g e
EXPRESSION OF GENETIC INFORMATION
MBT – CC201
Credit :4, Max. Marks: 100 [Sessional Marks: 25, Exam. Marks: 75] Time : 72 hours
UNIT – I Subject Coordinator : Prof. Pradip K. Chakraborti
Translation: mRNA, genetic code and its salient features. The structure of
tRNA. Genesis of –CCA. Adapter role of tRNA. Wobble hypothesis. Ribosome
as the site of protein synthesis. Structure and assembly. Polysomes.
Activation of aminoacids: aminoacylation of tRNA. Initiation, elongation and
termination of protein synthesis in prokaryotes and eukaryotes. Role of
Initiation and Elongation factors. Peptidyl transferese activity and peptide
bond formation. Translocation of ribosomes. Fidelity of protein synthesis:
GTPase timer. Bioenergetics of protein synthesis.
UNIT – II
Post-translational processing of nascent polypeptide: Acylation,

methylation, phosphorylation, sulfation, glycosylation, vitamin C-dependent
modifications, vitamin K- dependent carboxylation and proteolytic processing.
Inhibitors of protein and RNA synthesis. Role of antibiotics.
Protein Targeting to Organelles : Localization signals. Role of ER and Golgi bodies
in protein transport, transport vesicles, endocytosis and exocytosis. Protein
transport to nucleus, mitochondrion and chloroplast.
UNIT – III
Regulation of gene expression in prokaryotes : Constitutive and regulated

gene expression. Enzyme induction and repression in prokaryotes. DNA
binding motifs. Operon Theory: Lac, Trp, Ara and Gal operons. Role of
cAMP. Transcriptional termination control via alternate RNA conformations:
Attenuation. Regulatory cascades. Control of lytic and lysogenic cycles of
lambda phage. Flip flop circuits by genetic recombination. Role of sigma
factor in control of sporulation.
UNIT – IV
Regulation of gene expression in eukaryotes : General considerations.

Auto regulation, Role of methylation, Response elements. Britten-Davidson
models. Control by hormones and other primary messengers. Role of
receptor-ligand binding. Adenylcyclase, cAMP and other signal molecules.
Regulation of gene expression by chromatin structure. Regulation of Gal
genes in yeast. Switching of yeast mating types. Cytoplasmic regulation of
gene expression. control of translation. Role of mRNA stability. Hormonal
regulation of translation. Role of micro-nutrients. Regulation of rRNA
ribosomal protein synthesis.
19 | P a g e
MOLECULAR PLANT PHYSIOLOGY
MBT – CC202
Credit :4, Max. Marks: 100 [Sessional Marks: 25, Exam. Marks: 75] Time: 72 hours
Subject Coordinator : Prof. M.Z. Abdin

UNIT – 1
Photosynthesis, alternative respiration and Hexose monoprosphate

stunt : Light harvesting complexes and light reaction. The photosynthetic
carbon reduction cycle (PCR), C4 and Crassulacean acid metabolism (CAM)
pathway. Photo-inhibition and photorespiration. Synthesis, transport and
storage of starch. Cyanide resistant respiration. Oxidative Pentose phosphate
pathway.
UNIT – II
Mineral Nutrition in Plants : Importance of mineral nutrition in plant growth,

development and productivity. Criteria for the essentiality of mineral nutrients, and
their physiological functions. Nutrient uptake (active and passive uptake); active
transport and electrogenic pumps. Assimilation of mineral nutrients (nitrogen,
sulphur and phosphorus) and their physiological functions. Biological nitrogen
fixation : nif genes, nodulin genes and nodule development. Nitrogen and Sulphur-
use efficiency.
UNIT – III
Phytohormones, Photoreceptors and Dormancy : Structure, biosynthesis

and molecular mechanism of action of phytochromes (Auxins, Gibberellins,
Cytokinins, Abscisic Acid, Ethylene). Structure and functions of
brassinosteroids and polyamines.
Photoreceptors: structure and function of phytochromes and cryptochromes;
role in signal transduction. Flower and seed development. Seed dormancy
and germination. Types of seed dormancy and methods to overcome dormancy.
UNIT – IV
Stress and Post Harvest Physiology: Abiotic stresses (drought, submergence,

low and high salinity. temperature, salt and heavy metal stresses). Role of
LEA proteins in stress tolerance. Biotic stresses (insects and diseases), stress
induced gene expression. Molecular basis of senescence, ageing and programmed
cell death in plants. Molecular biology of fruit ripening and control of post - harvest
deterioration of fruits, vegetables and cut flowers.

20 | P a g e
PLANT TISSUE CULTURE
PLANT AND ITS APPLICATIONS
BIOTECHNOLOGY I
MBT––CC203
MBT 302
Credit
Credit :4, Max.
:3, Max. Marks:
Marks: 100100 [SessionalMarks:25,
[Sessional Marks:25, Exam.
Exam.Marks:
Marks:75]
75]Time : 72: hours
Time 50 hours
Subject Coordinator : Dr. Alka Narula

UNIT – I
In Vitro culture of plant cells and tissues : Concept and significance of

plant tissue culture, principles and methods. Introduction of techniques,
basic media, physical parameters, culture of various explants and possible in-
vitro responses. Single cell and cell suspension culture.
UNIT – II
In vitro differentiation : Organogenesis, embryogenesis, micropropagation,

haploids through anther and pollen culture, endosperm culture and
induction of triploids, nucellus culture, ovary ovule and embryo culture and
rescue of hybrids, floral bud culture, floral development in Arabidopsis and
Antirrhinum, culture of shoot primordia, stem and root culture, clonal fidelity
of regenerants. Bioreactors.
UNIT – III
Application of Tissue culture : Somaclonal variation and its application in

varietal improvement, Use of plant tissue culture technology in crop
improvement- selection for drought and salt stress tolerant plants;
development of herbicide and pesticide tolerant plants; induction of disease
resistant and insect tolerant plants. Synthetic seeds, Cryo-preservation for
conservation of plants.
UNIT – IV
Protoplast culture and Vector Independent Transformation : Isolation of

protoplasts and somatic hybridization. Applications of somatic hybrids and
cybrids in crop improvement. Methods of protoplasts and tissue
transformation; microprojectile bombardment, electroporation and
microinjection. The advantages and disadvantages of these techniques.

21 | P a g e
BIOTECHNOLOGY: ENVIRONMENTAL & ETHICAL ASPECTS
MBT––CC204
MBT 401
Credit
Credit :4, Max.
:3, Max. Marks:
Marks: 100100 [SessionalMarks:25,
[Sessional Marks:25, Exam.
Exam.Marks:
Marks:75]
75]Time : 72: hours
Time 50 hours
Subject Coordinator : Dr. Humaira Farooqi
UNIT – I
Ethical, Social and Biosafety aspects: Socio-economic and ethical aspects of biotechnology.
Environmental laws; Intellectual property rights; Objective of patent system, patentable
subjects and protection in biotech; Basic Principles of patent system, UPOV for plant
protection. GLP andGMP.
Objectives and levels of biosafety: Objectives; recombinant DNA safety; biological

containment; risk groups and risk analysis. Carategana Protocol; OECD guidelines. Govt of
India guidelines for r- DNA technology and GMO’s. Ecological impact and biosafety issues of
GM crops.
UNIT – II
Biotechnology and Environment Management: Bio-indicators and their applications in

environmental monitoring programmes, role of biosensors. Carry over effects of herbicides
and pesticides on human health. Bio-fertilizers: types of biofertilizers, production technology
for major biofertilizers (Rhizobium, Azotobacter, Azolla, Phosphate solubilizing microbes and
Mycorrhizae). Vermi-composting. Bio-pesticides: Development of biopesticides: management
of weeds, insect pests and diseases.
UNIT – III
Basic ecological concepts: Habitat ecology, systems ecology, synecology, autecology;

Ecosystem concept; Structure and functions of biotic and abiotic components; Energy in
ecosystems and environment; Energy exchange and productivity-food chains and food webs-
ecological pyramids, nutrient cycles and recycle pathways; Biomagnifications Population -
characteristics and measurement. Communities - habitats, niches, population dynamics,
species and individual in the ecosystem. Ecological succession - types and causes.
Research Ethics:
 Concept of Plagiarism.
 Reviewing literature.
 Identification of research problem and proposal writing..
UNIT – IV
Bioremediation: Conventional and advanced technologies for the treatment of sewage and
industrial effluents, bioremediation of xenobiotics: characteristics and classification of
recalcitrant xenobiotics, metabolic pathways involved in their biodegradation, factors
affecting biodegradation of xenobiotics. Phytoremediation and wasteland reclamation.
Biofuels; Generation of biofuels. Different Sources; Production of biodiesel and bioethanol.

Advantages of biofuels over petrol. Biofuels risks.
Biodiversity Conservation: Types of Biodiversity and Conservation. Afforestation

programmes: NAP and EDF schemes; Gene banks: Objectives and types of gene banks; Gene
flow monitoring.
Environmental Audit : Green audit and Energy audit

of any difficulties, students will contact the course coordinator. 22 | P a g e
BIO-STATISTICS
MBT – OE205
Subject Coordinator : Ehsan A. Khan
To be taught by Professor E.A. Khan

UNIT –I :
Arithmetic Mean, Median and Mode (Theory and simple numerical problem)
Measures of variation : Standard Deviation, variance, coefficient of variation,
properties (Theory and simple numerical problems)
Correlation : Types of correlation, methods of correlation, simple, multiple
and linear and non linear correlation, spearman’s correlation coefficient,
Rank correlation (Theory and simple numerical examples)
UNIT – II
Regression : Linear regression, curvilinear regression (for two variables X

and Y only), Regression lines by least square methods, Regression equations
of X on Y and Y on X only (Theory and simple numerical examples only)
UNIT – III
Tests of significance :Null hypothesis, standard error, level of significance,

Degrees of freedom, significance in mean for large samples, significance in
means for small samples (students t-test)/ Significance in ratio of two
samples. F-test (for difference between variance of two samples), chi square
test (Simple numerical examples and theory), Analysis of variance test
(ANOVA) for one and tow way classification. Signed Rank test, Dunnet’s-t-
test (Theory and numerical examples)
To be taught by a teacher from Department of Computer Science
UNIT – IV
Computer applications and data management :

Basics of computer
Input and output tools
Applications of computer in Biotechnology
 Data handling
 Data acquisition
 Data mining
23 | P a g e
PRACTICAL – MOLECULAR PLANT PHYSIOLOGY AND PLANT
TISSUE CULTURE
MBT – CC206
Credit: 8, Max. Marks: 200 [Sessional Marks: 50, Exam. Marks: 150] Time : 200 hours
1) Estimation of nitrite in biological samples.

2) Estimation of nitrate in biological samples by hydrazine reduction
method.
3) Assay of in vivo nitrate reductase activity in biological samples.
4) Estimation of chlorophyll ‘a’, ‘b’ and total chlorophyll from biological
samples by Hiscox and Israelstam method.
5) Extraction and quantification of proteins from plant tissues.
6) Isolation of acid phosphatase from germinated wheat and determination
of its activity.
7) Determination of phosphatase activity at different temperature, pH and
substrate concentration and calculation of Km and Vmax.
8) Preparation of MS and WB media.
9) Inoculation of seeds of Trigonella, Brassica and Cichorium sp. and
evaluation of their percent germination in in vitro conditions.
10) Study the regeneration potentials of juvenile explants (hypocotyls/
epicotyls) of Trigonella foenum graecum.
11) Standardization of regeneration protocol of lilium using bulb/ bulbscale/
leaf base.
12) Study the effect of 2,4-D in developing micropropagation protocol for
Linum using cotyledonary leaves.
13) In vitro endosperm culture.
14) Study differentiation potentials of nodal segment/ leaf/ petiole of
Tylophora indica.
15) Raising haploid cultures using anthers of Datura innoxia under in vitro
conditions.
16) Inoculation of nodal segment and leaf of Nicotiana tobaccum (from in vitro
source).
17) Subculturing of selected plant under in vitro conditions.
18) Establishment of cell suspension culture from the friable callus.
24 | P a g e
Seminars / Assignments
MBT – DCE207
Credit:02, Max. Marks: 50 Time : 36 hours
The students will be assigned topics in various areas of Biotechnology for

seminars and assignments. The assignments are to be submitted to the
mentors for evaluation at the end of semester. The evaluation of students for
seminars will be based on the quality of subject matter, templates and
presentation. The seminars will be attended by all the teachers of the
Department and individually evaluated. Participation of all the students in
seminars is compulsory and their attendance will be marked.
25 | P a g e
Semester - III
26 | P a g e
ANIMAL BIOTECHNOLOGY
ANIMAL BIOTECHNOLOGY
MBT– –CC301
MBT 301
Credit :3, :4,
Credit Max. Marks:
Max. 100
Marks: 100[Sessional
[SessionalMarks:25, Exam.Marks:
Marks:25, Exam. Marks:75]75] Time
Time : 72 :hours
50 hours
Subject Coordinator : Dr. Saima Wajid

UNIT – 1
Insect cell and mammalian cell culture :Primary and secondary cultures,
cell lines. Upscaling of anchor dependent and suspension cell cultures.
Expression of cloned genes in heterologous systems. General considerations.
Expression vectors. Promoters and other elements. Selection markers.
Production of bio-molecules by rDNA technology, expression of independent
and fused proteins, simple and glycosylated proteins. Choice of expression
system. Expression strategies. Construction of expression cassettes. Factors
effecting high level expression.
UNIT – II
Prokaryotic expression systems : E. coli, Bacillus and Streptopmyces:

General features and strong promoters. Natural and hybrid promoters. The
pIN series of vectors. Phage promoters. His and other tags. Secretory signal
and secretion of r-proteins to periplasm. Inclusion bodies.
Yeast as host for expression of foreign genes :Saccharomyces and Pischia.
Yeast plasmids. Yeast expression vectors. yEP, yIP, yRPs and yAC.
UNIT – III
Insect cells and Baculovirus Expression Vector system : Early and late
promoters. Advantages of polyhedron promoter. Indirect cloning in
baculovirus. Silkworm larva as biofactory for the production of r-proteins.
Mammalian cell expression system : Strong promoters. SV40 and Cos cells.
Shuttle vectors. Helper virus and binary vector system. BPV, EBV and BKV
promoters for development of expression vector. Vaccinia virus: Potential
applications and pros and cons of its use. Adeno and retrovirus based
expression vectors.
UNIT – IV
Proteomics and r-Proteins: In vitro cell free protein synthesis: wheat germ S-
30, Rabbit reticulocytes; In vivo protein synthesis: frog oocyte system; Protein
engineering; Interactome: Protein-protein interactions. Proteome analysis.
Isolation and purification of r-proteins. Bioactivity of r-proteins. Strategies for
commercial production. Gene dose and expression levels.
Commercially available recombinant proteins. Production of GH, insulin;
TPA, gonadotropins, HBsAg and other biomedical products by r-DNA
technology.
27 | P a g e
PLANT BIOTECHNOLOGY
MBT – CC302
Subject Coordinator : Prof. M.Z. Abdin

UNIT – I
Transgenic technology: Agrobacterium, the natural genetic engineer.

Molecular mechanism of Agrobacterium mediated genetic transformation of
plants. Cloning (Plasmid, Pragemids, Cosmids, BACs and YACs) and
expression vectors (co-integrative vectors, Binary vectors, Gateway vectors,
multicistronic vectors) for plant genes, structure, types, their advantages
and disadvantages. Plant Promoters types: Constitutive, tissue specific and
inducible; their applications in transgenic technology. Scorable and
selectable markers. Transgene integration, copy number, stability,
expression, silencing and inheritance in transgenic plants. Excision system
for removal of undesirable genetic elements (selection marker gene) from
transgenic plants.
UNIT – II
Plastome engineering and applications: Introduction of plastome and

vectors used in plastome engineering. Transformation of plastids and their
use as bioreactors for the production of various metabolites of therapeutic
importance. Plastome engineering for development of male sterile plants and
edible vaccines.
UNIT – III
Value addition of plants through genetic engineering-I: Transgenic plants

for production of therapeutics and plantibodies; production of edible vaccines
through transgenics; genetic engineering for quantitative and qualitative
improvement of carbohydrates, proteins, micro-nutrients, vitamins and
secondary metabolites.
UNIT – IV
Value addition of plants through genetic engineering-II : Transgenics

with increased shelf-life of fruits and flowers. Use of genetic engineering
technology for improved lipid metabolism. Herbicide plants through
transgenic technology. Genetic engineering for insect-pest resistance and
generation of stress tolerant plants for enhanced productivity.

28 | P a g e
IMMUNOLOGY
MBT – CC303
Credit: 4, Max. Marks: 100 [Sessional Marks:25, Exam. Marks: 75] Time : 72 hours

UNIT – I
Overview of immune system: Principles of Immunology – Origin of Immunology

and its evolution.
Cells and organs of immune system. T and B cells, macrophages, dendritic cells, NK
cells. Primary, secondary and tertiary lymphoid organs.
Types of immunity - Innate and adaptive, Humoral and cell-mediated, Active and
passive, PAMP:TLR, Clonal selection theory.
Immunological memory, Antigens and immunogens, B and T cell epitopes; Haptens.
Structure and functions of antibodies. Classes of immunoglobulins. CDRs,
immunoglobulin fold. Valence, affinity and avidity. Antibody variants - Isotypes,
allotypes and idiotypes.
UNIT – II
Recognition of antigens: The immunoglobulin genes: organization and assembly;

generation of immunological diversity; Allelic exclusion. Major histocompatibility
complex(MHC): structure and organization of MHC Class I and Class II molecules.
Antigen processing and antigen presentation. T cell Receptor: αβ and γδ receptors;
Costimulatory molecules; Superantigens. B cell activation and maturation;B1B and
B2B cells; T-cell dependent and T-cell-independent antigens. T cell development and
activation. Cytotoxic T cell mediated killing. Complement system and mechanism of
its fixation. Complement deficiencies.
UNIT – III
Immune effector mechanisms:Products and factors produced by T-cell

activation.Cytokines and chemokines-Interleukins, interferons, growth factors.
Antigen-antibody interactions: equilibrium approach. Immunoprecipitation,
Agglutination, Immunoelectrophoresis, Immunofluorescence, RIA, ELISA: indirect,
sandwich and competitive; Elispot assay. Cytotoxicity assay: MTT assay and Trypan
blue; MLR, Hemolytic plaque assay
Flow cytometry and FACS; Confocal microscopy and imaging. MHC inbred, nude,
congenic, syngenic and knockout mice – Utility
UNIT – IV
Immune system in health and disease: Immunological tolerance.
Autoimmunity and associated disorders. Allergy and hypersensitivity.
Transplantation immunology - Graft rejection, graft versus host reaction.
Tumor immunology, cancer immunotherapy. Immune response to infectious
diseases – viral, bacterial, protozoal. Immunosuppression - immunodeficiency
diseases (eg.AIDS).
29 | P a g e
BIOTECHNOLOGY AND HUMAN HEALTH
MBT – CC304
UNIT – I
Hybridoma Technology: Production of murine monoclonal antibodies (MoAbs)-Fusion
strategies, HAT Selection; Strategies for production of human MoAbs-Humanization and
antigenization of MoAbs-Chimeric, CDR-grafted, SDR-grafted, veneered MoAbs.
Antibody Engineering: Antibody fragments, Antibody gene cloning; Expression of
recombinant antibody genes; Next generation display technologies for production of
antibodies in vitro; Combinatorial libraries and phage display libraries; Bispecific and bi-
functional antibodies; Immunoconjugates; Catalytic antibodies.
Clinical applications of MoAbs and engineered antibodies: In diagnostics, therapeutics
and other uses.
UNIT – II
Microbial Biotechnology and its applications: Components of culture media, synthetic
defined media, complex media, supportive media, enriched media, selective media,
differential media; Pure culture isolation by streaking, serial dilution and plating methods;
Cultivation, maintenance and stocking of pure cultures, cultivation of anaerobic bacteria;
Control of microorganisms- physical and chemical agents; Antibiotics and antiviral agents;
Use of prokaryotic and eukaryotic microorganisms in biotechnological applications;
Genetically engineered microbes for industrial application-Bacteria and yeast; Recombinant
microbial production processes in pharmaceutical industries-Streptokinase, Hepatitis-B
recombinant vaccines.
UNIT – III
Fermentation and Food microbiology: Scope, classification based on nature of the
products and kinetics of cell growth; Substrates for fermentation; Isolation and preservation
of cultures; Design of fermenters, various types of fermenters; Common problems and trouble
shooting; Downstream processing; Purification of products; Probiotics and fermented foods;
Applications
Cell and enzyme immobilization: Methods of immobilization, kinetics and uses of
immobilized enzymes; Bioreactors using immobilized enzymes; Applications of immobilized
enzymes in medical science and Industry; Biocatalyst technology, biosensors and analytical
applications.
UNIT – IV
Diagnostics: Nucleic acid and protein based diagnostic.
Strategies of Vaccine development: Traditional and new generation vaccines; Live
vaccines-(Polio, Rotavirus); Recombinant vaccines (Hepatitis B); Sub unit, VLPS and DNA
vaccines; Reverse vaccinology; Newer concept: Rational design based on Structural
biology & System vaccinology approach
Preclinical and clinical evaluation of vaccines
Gene therapy: Concept, principle, strategies and applications.
Stem Cells, Tissue Engineering and Regenerative medicine : Types of stem cells, Isolation
of stem cells and cryopreservation; Therapeutic cloning, Nuclear reprogramming; Induced
pluripotent stem cells; Ethical issues in stem cell research; clinical
applications(cardiovascular disease, cancer, spinal injury); Cord blood banking; Tissue
engineering: Technology in general and applications; Regenerative medicine .

30 | P a g e
BIOINFORMATICS
MBT – OE305
Subject Coordinator : Dr. Alka Narula

UNIT – I
Introduction to Bioinformatics: Introduction and branches of Bioinformatics. Aim, scope
and research areas of Bioinformatics.
Databases in Bioinformatics: Introduction. Biological databases. Classification format of
biological databases. Biological database retrieval system.
Biological Sequence Databases:
 National Center for Biotechnology Information (NCBI): Tools and Databases of
NCBI,Database Retrieval Tool, Sequence Submission to NCBI, Nucleotide Database,
Protein Database, Gene Expression Database.
 EMBL Nucleotide Sequence Database (EMBL-Bank): Introduction, Sequence
Retrieval,Sequence Submission to EMBL, Sequence analysis tools.
 DNA Data Bank of Japan (DDBJ): Introduction, Resources at DDBJ, Data Submission
atDDBJ.
 Protein Information Resource (PIR): About PIR, Resources of PIR, Databases of PIR,
Data Retrieval in PIR.
 Swiss-Prot: Introduction and salient features.
UNIT – II
Bioinformatics resources on the internet.
Computational methods for sequence analysis: Pairwise and multiple sequence alignment
for DNA and protein sequences. Local and global sequence similarity.
Methods of sequence alignment: Dot matrix method, Dynamic programming method and
Heuristic method.
Scoring matrices: PAM, BLOSUM, Gonnet, Lookup tables.
Tools for similarity search and sequence alignment: BLAST and types, FASTA.
UNIT – III
Genome analysis and Gene identification: Sequencing, Assembly, Annotation,
Sequencing pipelines and databases.
Genome comparison and analysis.
Molecular Phylogeny: Methods of Phylogeny, Software for Phylogenetic Analyses,
Consistency of Molecular Phylogenetic Prediction.
Comparative genomics: Homologs, Paralogs and orthologs; Synteny; Comparative genomics
of Arabidopsis and Brassica rapa / Chimpanzees and human.
Structural analysis of Nucleic acids: Tools for prediction and designing.
UNIT – IV
Application tools: Primer designing.
Molecular imaging and design: CADD, QSAR.
Tools for molecular mapping: QTL, minisatellites, SNP's.
Mapping techniques: JoinMap, MapQTL, LOD Score method for estimating recombination
frequency.
Prediction of 3 dimensional structures of proteins: protein secondary and tertiary
structure prediction by using techniques: Chou-Fasman/GOR method, comparative
modeling, Threading and ab initio structure prediction.
Systems Biology – Concept and applications

31 | P a g e
PRACTICAL: IMMUNOOGY, ANIMAL AND PLANT
BIOTECHNOLOGY
MBT – CC306
1) Preparation of Dulbecco’s Modified Eagle’s medium for mammalian

cell culture.
2) Revival and maintenance of CHO cells.
3) Trypsinization and storage of CHO cell line.
4) Cryopreservation of mammalian cell (CHO).
5) Transfection of mammalian cell (CHO) by calcium phosphate co-
precipitation method.
6) Ammonium sulphate precipitation of antibodies in serum.
7) Preparation of antigen -adjuvant (FCA) emulsion.
8) Immunization of mice with antigen-adjuvant formulations.
9) Collection of blood from mice and separation of serum.
10) Analysis of antibodies raised in immunized mice by ELISA
11) Determination of immunogenic proteins by Western Blot Analysis.
12) Preparation of synthetic seeds and in vitro germination.
13) Isolation and culture of protoplast.
14) Isolation of total plant genomic DNA by Doyle and Doyle’s method
from different plant samples.
15) Quantitative and Qualitative analysis of plant genomic DNA.
16) Extraction of RNA from plant samples.
17) Quantitative and qualitative analysis of RNA sample.
18) Kanamycin sensitivity test in leaf segments.
19) Semi-solid and liquid cultures of Agrobacterium tumefaciens
20) Growth kinetics of Agrobacterium tumefaciens in liquid culture.
21) Transformation of explants with Agrobacterium tumefaciens and
regeneration of explants.
32 | P a g e
Seminars / Assignments
MBT – DCE307
Credit:02, Max. Marks: 50 Time : 36 hours
The students will be assigned topics in various areas of Biotechnology for

seminars and assignments. The assignments are to be submitted to the
mentors for evaluation at the end of semester. The evaluation of students for
seminars will be based on the quality of subject matter, templates and
presentation. The seminars will be attended by all the teachers of the
Department and individually evaluated. Participation of all the students in
seminars is compulsory and their attendance will be marked.
33 | P a g e
Semester - IV
34 | P a g e
DISSERTATION
MBT – CC401
1. Research Project and submission of dissertation.

2. Preparation and submission of Review article
35 | P a g e

M.Sc. Syllabus 2020-2022

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2.

VISION OF THE DEPARTMENT

 Molecular biology and regulation of gene expression in infectious and non-

Confocal microscopy: Our department is also having functional Confocal Laser

CENTRE FOR TRANSGENIC PLANT DEVELOPMENT:

EXTRAMURAL RESEARCH FUNDING

UGC-Special Assistance Program

Universities are cradles of innovation and knowledge creation. Research in universities

S.No Name Designation Specialization

Plant Biotechnology and

4 Dr. Alka Narula Asst. Professor Plant Biotechnology

Protein Biochemistry and

Clinical Genomics and

Nano-enabled Interfaces for

8 Dr. Javaid Ahmad Sheikh Asst. Professor Immunology

Host Pathogen Interaction and

INSA Senior Stress Physiology and

Molecular Biology and

Dr Alka Narula is Assistant Professor in the Department of Biotechnology. She

DR. HUMAIRA FAROOQI

Dr. Humaira Farooqi did her Ph.D. in Biochemistry from Department of

DR. JAGRITI NARANG

Dr. Jagriti Narang is Assistant Professor at Department of Biotechnology, Jamia

DR. JAVAID AHMAD SHEIKH

Dr. MAIRAJ AHMED ANSARI

Dr. Mairaj Ahmed Ansari, completed Ph.D. in Biotechnology (molecular-immunology

Prof. Malik Zainul Abdin is Professor in the Department of Biotechnology, School of

PROFESSOR PRADIP KUMAR CHAKRABORTI, HoD

PROFESSOR PRATIMA RAY

Professor Pratima Ray teaches at the Department of Biotechnology, Biochemistry and

DR. SAIMA WAJID

Dr. Saima Wajid joined Department of Biotechnology, SCLS, Jamia Hamdard

ADMINISTRATIVE AND TECHNICAL STAFF

S. No. Name Designation

1 Mr. Ajmal Abbasi Sr. Assistant

2 Mr. Anish Cherian Assistant

3 Ms. Noor Fatima Technical Assistant

4 Mr. Syed Naved Quadri Research Assistant

5 Mr. Mubarak Khan Lab Assistant

6 Mr. S. Zafar Nazish Lab Assistant

7 Mr. Diwan Mohammad Lab Attendant

8 Mr. Satish Chand Lab Attendant

9 Mr. Sharfuddin Lab Attendant

10 Mr. Hemraj Mali

S.No Name Designation

1 Mr. Wajid Ali Technical Assistant

2 Mr. Islamuddin Office cum Lab Attendant

3 Mr. Murad Bux Lab Attendant

STUDENT SUPPORT FACILITIES

CENTRAL INSTRUMENTATION FACILITY (CIF)

HAKIM MOHAMMED SAID LIBRARY

 Over 2 lacs books.

FACULTY LEVEL ANTI RAGGING SQUAD

EQUAL OPPORTUNITY CELL

www.jamiahamdard.edu and www.jamiahamdard.ac.in are websites for resources of

HAMDARD CONVENTION CENTRE

Beautifully designed and centrally air-conditioned Convention Centre has attracted

University has following hostels for boys and girls:

 Anne Marie Schimmel Hostel - PG girls

SPORTS AND CULTURAL ACTIVITIES

NATIONAL SERVICE SCHEME (NSS)

NATIONAL CADET CORPS (NCC)

STUDENTS AID FUND