ISOLATION AND BIOCHEMICAL

CHARACTERIZATION OF BACTERIAL
DIVERSITY FROM DIFFERENT SOIL REGION OF
SURENDRANAGAR DISTRICT

THESIS

Submitted for the Degree of

MASTER OF SCIENCE
In
Microbiology

In the Faculty of Science

C.U. Shah University
Surendranagar

By

Makwana Mohitbhai Keshabhai

[En.no: 20SC509016]

Under the Supervision of

Ms. Maheshwari R. Patadiya

Assistant Professor
C. U. Shah Institute of Life Sciences
C. U. Shah University
Surendranagar-363030
Gujarat, India

April -2022
 C.U. Shah Institute of Life Sciences
Faculty of Sciences
C.U. Shah University, Wadhwan city
Website: www.cushahuniversity.ac.in

/04 /2022

CERTIFICATE

This is to certify that the work incorporated in the dissertation thesis entitled
“Isolationand biochemical characterization of bacterial diversity from different

soil region of Surendranagar district” by Makwana Mohitbhai

Keshabhai (20SC509016) carried out at Department of Life Sciences, C.U.
Shah University, Wadhwan, comprises the result of independent and the original work
for the partial fulfilment of the degree of M.Sc. in Microbiology.
I further certify that this work did not form any part of work published or
unpublished.

Ms. Maheshwari R.Patadiya Ms. Ankita G. Murnal

(Research Guide) Head of Department,
Department of Life Science, Department of Life Science,
C. U. Shah University, C. U. Shah University,
Wadhwancity – 363030, Wadhwancity – 363030,
Gujarat, India. Gujarat, India.
/ 04 / 2022

DECLARATION
C.U. Shah Institute of Life Sciences
Faculty of Sciences
C.U. Shah University, Wadhwan city
Website: www.cushahuniversity.ac.in
I am Makwana Mohitbhai Keshabhai &20SC509016 Student of
Department of Life Science, C. U. Shah University,Wadhwancity hereby undertaken
that the work presented in the dissertation work report entitle “Isolation and
biochemical characterization of bacterial diversity from different soil region of
Surendranagar district” comprises the result of independent and original work
carried out for the partial fulfillment of the degree of M.Sc. in Microbiology and not
subjected to published/present elsewhere without prior permission.

Date: /04/2022 Makwana Mohitbhai Keshabhai

Place: Wadhwancity 20SC509016

ACKNOWLEDGEMENT

Getting through my postgraduate dissertation would never have been possible if I had
to pursue it alone. First and foremost, I must thank the Almighty for guiding me in
every step to complete my work properly.

It is a great pleasure to acknowledge my deepest thanks and gratitude to my

supervisor Ms. Maheshwari Patadiya Assistant Professor, Department of life
science, C U Shah University. Without her Support, Diligent Guidance, and
Encouragement, this research would never have come to reality. I would like to thank
you very much Mam, for inspiring me in every step of the way and for believing in
me.

I’m much obliged to Ms. Ankita Murnal, Ms.Unnati Yagnik, Ms.Parthavi Akheja,
Ms.Chandani Marvaniya, Mr. Meet Khokhar, Mr.Kuldipsinh Parmar Assistant
Professors who had given me valuable suggestions regarding experimental settings.I
would like to thank Mr. H.T.Patel sir and D.B.Patel sir, Lab Assistant for their
support and Guidance.

I would like to extend my appreciation to Dr. Dharamvirsinh Parmar, (Dean, FoS)

for his constant support.

I would also like to acknowledge my colleagues Zala Priyanka, Patel Bhoomi,

Poojarani Singh, Ekta Padheriya, Who had given me constant support and
Countless help during My Laboratorywork.

Most importantly, I could never have preserved without my family who offered me
their encouragement and listened to my frustration with patience. I am grateful for
having them in my life.

Date: /04/2022

TABLE OF CONTENTS
Page
Chapter Name
No.
1. Introduction 1
Review of literature 15
 2.

3. Aims and Objective 19

4. Materials and Methodology 20

5. Results 30

Discussion 41

Conclusion 42

References

LIST OF TABLES

TABLE PAGE
TITLE No.
No.
Table 1.1 Soil Colour 8

Table 4.2.1 Different Soil sample 21

Table 4.2.2.1 Preparation of N-agar Media 22

Table 4.2.2.2 Preparation of MSA Media 23

Table4.2.2.3 Praparation of MHA Media 29

Table 5.1.1 Different Soil Sample Isolates 30

Morphological characterization of sample-A
Table 5.1.2 31
isolates colonies
Gram staining result for sample-A isolates
Table 5.1.3 31
colonies
Morphological characterization of sample-B
Table 5.1.4 32
isolates colonies
Gram staining result for sample-A isolates
Table 5.1.5 33
colonies
Morphological characterization of sample-C
Table 5.1.6 34
isolates colonies
Table 5.1.7 Gram staining result for sample-C isolates colonies 34

Table 5.1.8 Colony count of different soil sample 35

Table 5.1.9 Biochemical characrerization of sample-A 35

Table 5.1.10 Biochemical characrerization of sample-B 36

Table 5.1.11 Biochemical characrerization of sample-C 37

Table 5.2.1 Zone of Inhibition sample-A colonies 39

Table 5.2.2 Zone of Inhibition sample-B colonies 39

Table 5.2.3 Zone of Inhibition sample-Ccolonies 40

LIST OF FIGURES
FIGURE PAGE
TITLE
No. No.
Fig-1.1 Soil Aggregates 2

Fig:1.2 Sandy Soil 5

Fig: 1.3 Loamy Soil 5

Fig:1.4 Clay Soil 6

 Fig:4.2.2.1 M-R test 25

Fig:4.2.2.2 Indole test 26

Fig: 4.2.2.3 Catalase test 27

Fig:4.2.2.4 Citrate test test 27

Fig:4.2.2.5 V-P test 28

Fig:5.1 Reults of M-R test 37

Fig:5.2 Results of Indole test 37

Fig:5.3 Results of Citrate test 37

Fig:5.4 Results of V-P test 37

Fig: 5.5 Results of Catalase test 38

LIST OF ABBREVIATIONS

CFU: Colony Forming Unit

Dm : Diameter

QC : Quality Control

NLWR: Non Limiting Water Range

Dw: Distilled water

PM : Primary Metabolites

SM : Secondary Metabolites

ZI : Zone of Inhibition

ABSTRACT
Antibiotics are most important commercially used secondary metabolites, produced
by many microorganisms. i.e.,bacteria and fungi are employed in wide range. Most
important antibiotics used today are of microbial origin. In present study, Soil
samples from different area or region were collected i.e., the sampling is classified
based on its micro and macro environment. Farm Soil (Agriculture Soil), River
Shoer Soil and Industrial area Soil were collected. from a local and analyzed for the
antibiotic production. Primary Screening can be bacterial colonies were isolated to
soil and check the morphological characteristics, biochemical reaction and
identification of the bacterial sp. Many bacterial sp. can be isolated in soil such as
Bacillus sp, S.aureus sp., E.Coli sp., Pseudomonas Sp., Klebsiella Sp., were
showed in Soil. In this study by to identification and checked for antibiotics activity
 against some common gram positive and negative bacteria where Staphylococcus
aureus and Escherichia Coli.to produces the Zone of Inhibition. These stydy
suggests for different region are soil are many microorganisms were isolated and
these microbial starin very helpful for all living organisms. The S. aureus Sp. have a
potential to produce antibiotics and can be used to control the microbial growth in
future.

KEY WORDS :- Zone of Inhibition, Primary screening, Secondary metabolites,