Spotlight Ascentis Express 5 micron: A Fused-Core Particle HPLC

High-Throughput
Column for Faster HPLC Separations with No
Analysis of Volatile Backpressure Concerns
Impurities in
Sustainable Packaging Published on: June 1, 2012
using SIFT-MS Carmen T. Santasania, Wayne K. Way, Sigma-Aldrich Supelco
The Application Notebook
Automated Sample
Prep in Regulated and The Application Notebook, The Application Notebook-06-01-2012, Volume 0, Issue 0
Nonregulated Labs Just
Got Easier

What is Chemical
Composition Ascentis Express 5 micron columns provide a new choice for improving the performance of traditional HPLC systems.

Distribution in Based on Fused-Core particle technology, Ascentis Express provides the benefits of high speed and high efficiencies

Polyolefins, and Why without the concerns of smaller particle columns. Due to the high efficiencies at low backpressures, Ascentis Express

Should It Be Part of 5 micron can benefit conventional HPLC users with no drawbacks.

Your HT GPC Analysis?

Ascentis Express 5 micron columns provide a new choice for improving the performance of traditional HPLC

Essentials of LC systems. Based on Fused-Core particle technology, Ascentis Express provides the benefits of high speed and high
Troubleshooting, Part II, efficiencies without the concerns of smaller particle columns. Due to the high efficiencies at low backpressures,
Continued: More Ascentis Express 5 micron can benefit conventional HPLC users with no drawbacks.
Misbehaving Retention
Times The new Fused-Core particle consists of a 3.3 μm solid core and a 0.6 μm porous shell. A major benefit of the
Fused-Core particle is the small diffusion path (0.6 μm) compared to conventional fully porous particles. The short
Application of Porous diffusion path reduces axial dispersion of solutes and minimizes peak broadening. In fact, Ascentis Express 5
Layer Open Tubular micron columns are able to achieve greater speed and efficiency than any other 5 micron particle based column.
Columns: Beyond This means that Ascentis Express 5 micron becomes the standard column for all of your 5 micron based methods.
Permanent Gases
Beyond the new standard column for all 5 micron based methods, Ascentis Express 5 micron is an excellent
choice for bioanalytical LC–MS methods. The Ascentis Express 5 micron excels under high flow rates and the high
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throughput demands of these methods. Furthermore, the large particle format provides an extremely rugged HPLC

Liquid Chromatography column. Available in 17 dimensions from 20 mm × 2.1 mm i.d. to 250 mm × 4.6 mm i.d. and two introductory

(LC/HPLC) phases, C18 and F5, the conversion of current methods is seamless.

Gas Chromatography
(GC)

Sample Preparation
Table I: Summary of efficiency, backpressure, and speed for Figure 1
Mass Spectrometry
Experimental Conditions
Pharmaceutical
Analysis
Shown in Figure 1 are HPLC chromatograms of a mix of polar and non-polar analytes using both the new Ascentis

Environmental Analysis Express 5 micron and a popular traditional 5 micron column. The chromatogram illustrates the high speed and
high efficiency capabilities of the Ascentis Express at low back pressures. Table I provides a summary of the
Food and Beverage performance parameters of Figure 1.
Analysis

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Figure 1: HPLC chromatogram of a mix of polar and non-polar analytes on Ascentis Express. Column: Ascentis
Do Not Sell My Personal
Express C18, 15 cm × 4.6 mm i.d., 5 μm particles. Mobile Phase: 50:50 water:acetonitrile; temperature: 35 °C;
Information
flow rate: 1.50 mL/min; detection: UV, 250 nm; injection vol.: 5 μL. Peaks: 1. Uracil, 2. Acetophenone, 3. Benzene,
LCGC Staff
Privacy Policy 4. Toluene, and 5. Naphthalene.
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Terms and Conditions
Conventional HPLC users can obtain an immediate increase in efficiency with this "drop-in" replacement column.
No need to change column dimensions, flow rates, mobile phase conditions, or sample prep. The Ascentis Express
"5" can improve the performance of all of your HPLC systems.

Conclusion

Ascentis Express 5 micron with Fused-Core particles is an excellent choice for standardizing all 5 micron methods.
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and Chromatography Online. The combination of low backpressures and high efficiencies allows for greater column length and flow rate
All rights reserved. flexibility than offered by any 5 micron column currently available.

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC. Fused-Core is a registered trademark of Advanced
Materials Technology, Inc.

Supelco/Sigma-Aldrich

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tel. (800) 359-3041, (814) 359-3041

Website: sigma-aldrich.com/analytical