RATIONALIZATION – PREFINAL LECTURE EXAMINATION

December 11, 2020

What is the main component of neutrophil azurophilic granules? MYELOPEROXIDASE

The intense acidophilia of eosinophil granules is due to crystalloid bodies which also confer refractivity.
FALSE

Which describes a function of eosinophils? They release arylsulfatase and histaminase from their
specific granules at sites of allergic reactions. They are initially activated by IgG and IgA interactions.
Their granules neutralize vasoactive effects of basophil granules.

To increase IgE synthesis, CD40L on B lymphocyte surfaces interacts with its complementary receptor,
CD40, on basophil cell surfaces. FALSE

The following are effects of basophil degranulation, except: Decreased vascular permeability

Large granular lymphocytes (LGL) are otherwise known as: NK CELLS

Which statement is incorrect about T lymphocytes? T cell receptors (TCRs) are unique proteins that
are present only in the fully mature lymphocyte.

The following are cell surface markers for natural killer cells except: CD24

Which of the following functions as a phagocyte receptor for bacterial uptake in phagocytosis? CD18

Which correctly describes monocyte functions? As macrophages, their oxidative burst is less dramatic
than those in neutrophils.
Heinz Bodies are seen on the Wright stained smears. They are precipitated hemoglobin found at the
periphery of the red blood cell. 1st statement is false, 2nd statement is true
In heinz body preparation with acetylphenylhydrazine, the amount of oxygenation is determined by the
amount of whiffling. Because of this capillary blood is recommended. 1st statement is true, 2nd
statement is false

Which of the following statement/s is/are true?

I. The red cell zinc present in the RBCs would emit light at 415nm.
II. The current produce is directly proportional to the umol of zinc protoporphyrin/ mol heme.
III. This result is affected by hematocrit II only

Autohemolysis test is increased in Hereditary Spherocytosis. Defibrinated whole blood is needed for this
test. Both statements are true

Sodium bisulfite is a strong reducing agent for Sickle Cell tests. It deoxygenates hemoglobin. 1st
statement is false, 2nd statement is true
A CBC result from DxH 800 showed elevated result of basophils in its differential count. To confirm, the
medical technologist prepared a blood film for staining. Upon observation under the microscope, there
was poor staining of the WBC granules which lead to a difficulty in doing a manual differential count.
What is the likely cause of this error in PBS staining? Inadequate fixation

A peripheral blood smear appeared to be grainy and after staining this was observed under the
microscope: (PICTURE OF AUTOAGGLUTINATION) What is the resolution for this problem? Warm
blood @37C for 15 minutes

What would be the likely effect of this EDTA-induced phenomenon? (PICTURE OF PLATELET
SATELLITOSIS) pseudothrombocytopenia

When a stained blood film is viewed under the microscope, the medical technologist noticed that the
red blood cells appear bright red while the eosinophils are sparkling brilliant red. What is the most likely
cause? Insufficient staining

When a stained blood film is viewed under the microscope, the medical technologist noticed that the
red blood cells appear blue and the granules of the neutrophil appear large and prominent. What is the
most likely cause? Thick smear
Characteristics of a well-made peripheral blood smear:
I. Terminate in a straight feathered end
II. Smear narrower than the slide
III. Gradual transition from thin to thick areas
Which of the statements above are correct? I and II

A CBC result has a WBC count of 0.54 x103/ul. What would be the next course of action? Prepare a
buffy coat

A medical technologist makes wedge smears that are too short. What would be the probable cause?
Spreader slide pushed quickly

Irene, a medical technologist was assigned in the Hematology Department. During her shift she received
an influx of CBC results for PBS staining. Which of the following instruments should Irene use best so she
can finish at the given turnaround time? Dip-type instruments

A medical technologist received a specimen for CBC with STAT written on the request. Upon reviewing
the results, the specimen should be reviewed further by making a PBS. Which of the following
instruments should the medical technologist use? Both of the instruments above

A medical technologist received a specimen for CBC with reticulocyte count. Given the following data:
Hematocrit= 30%
RBC count= 2.9 x 10^12/L
Reticulocytes in 1000 RBCs= 50
Compute for the following: (answer in figures only in two decimal places with their units if applicable)
1. Reticulocyte Count
2. Corrected Reticulocyte Count
3. Reticulocyte Production Index
4. Based on the computed values, does it indicate an adequate bone marrow response? (YES or NO) 1.)
5%; 2.) 3.33%; 3.) 1.65; 4.) NO
Increased levels of glucose in the blood may inhibit staining of reticulocytes. Heinz bodies will also stain
supravitally thus, iron stain is performed to confirm its presence. 1st statement is true, 2nd statement
is false

When using a calibrated miller disk, RBCs should not be overlapping. Cells that touch the top and right
lines in both squares are not counted 1st statement is true, 2nd statement is false

In doing manual cell counts, the volume of the counting chamber 0.1 cubic millimeter is multiplied by 10
to give the contents of 1 millimeter cubed blood sample. The value 10 is considered as which of the
following? Depth correction factor

Which term is a hemocytometer ruling? Speirs-Levy

Which of the following statements describe the general purpose of a diluting fluid in manual cell count
procedure? Disperses other blood cells to facilitate the counting of specific cells

A laboratory technologist performed a manual red blood cell count on an Improved Neubauer
hemocytometer. Result in SI unit revealed 5.65 X 10 raised to the power 12 per liter. Which of the
following figures is the correct equivalent result in conventional unit? 5,650,000 cells per cubic
millimeter; 5.65 X 10 raised to the power 6 per millimeter cubed

Which magnification is used in doing a manual WBC count? 10X

Which of the following statements describe the accuracy in doing a manual WBC count? The variation
in the counts from each square should not differ by greater than 10 cells; The difference between the
lowest and the highest count in the eight squares should not differ by more than 15 cells
The presence of this cell in the blood sample can falsely elevate the manual WBC count result. Which of
the following descriptions of cell is correct for this cell? Cell has a paler and polychromatophilic
cytoplasm. Nucleus is degenerated or pyknotic

If the initial WBC count of the blood sample is <3.0 X 10 raised to the power 9 per liter, what should be
done to obtain an accurate manual WBC count result? A greater area is counted totaling to 9
millimeter squared instead of 4 millimeter squared; A lower dilution (1:10) of blood should be used

Which of the following is described as increased in the number of leukocytes accompanied by an

absolute increase in one or another type of WBC? Pathologic leukocytosis

A decrease in lymphocyte count is correctly termed as what? Lymphopenia