Assignment sheet

U.V-Vis Spectroscopy

1. 2.5 X 10-4 M solution of a substance in a 1 cm length cell at λmax 245 nm has

absorbance 1.17, Calculate max for this transition.
2. When a UV light is passed through the given solution, the radiant power is reduced to
50%, Calculate the absorbance.
3. A 0.01 M solution of a compound transmits 20% of the radiation in a container with
path length equal to 1.5 cm. Calculate the molar extinction coefficient of the
compound
4. A substance in a cell length ‘l’ absorbs 20% of the incident light. What fraction of
incident light will be absorbed in a cell of length 5l?
5. For a solution of organic ketonic compound (C10H16O) in hexane in a 10 cm cell, the
absorbance was found to be 2.52. What is the concentration of the organic compound
(Given max = 14)
6. When an incident beam of wavelength 3000 was allowed to pass through 2 mm
thick pyrex glass , the intensity of radiation was reduced to one tenth of its incident
value. What part of the same beam will be transmitted through 1 mm thick same
pyrex glass sheet.
7. When a beam of light (5000 ) was allowed to pass through 4 mm thick glass sheet,
the intensity of transmitted light was reduced to 20% of the initial value. What
percentage of light of the same radiation will be absorbed by 2 mm thick glass sheet?
8. What do you mean by a good solvent in UV spectroscopy and what is its effect on
absorption maximum?
9. For which isomer, π π* transition occurs at high wavelength and higher
extinction coefficient: cis-stilbene or trans-stilbene.
10. For the detection of aldehydes and ketones, which transition is more authentic,
π π* or n π* and why?