LECTIN CORRESPONDING FUNCTIONS OF IL, HLA MARKERS Anti-A Dolichos biflorus IL-1 Fever HLA-B7 Multiple Sclerosis Anti-B Griffonia simplicifolia IL-2 T cell growth HLA-B8 Grave’s disease, Anti-H Ulex europaeus Myasthenia gravis, Anti-M Iberis amara Type 1 DM Anti-N Vicea graminea/ Bauhinia variegata/ Bauhinia purpura IL-3 Multi-colony stimulating HLA-B27 Ankylosing spondylitis, factor Reiter’s syndrome AGGLUTINATION REACTION GRADING IL-4 Class Switching from IgM HLA-DR2 Goodpasture’s 0 No Agglutination/ Hemolysis to IgG, IgE syndrome, Narcolepsy W+ Tiny agglutinates w/ turbid background IL-5 Activate Eosinophils HLA-DR3 Celiac, SLE, Type 1 DM 1+ Small agglutinates w/ turbid background IL-6 CRP production HLA-DR4 Pemphigus, 2+ Medium agglutinates w/ clear background Rheumatoid Arthritis, 3+ Several large w/ clear background Type 1 DM 4+ One solid agglutinate IL-8 Stimulator of Eosinophils IL-12 Enhance NK Cells DISEASE CORRELATED CLUSTER OF DIFFERENTIATION T CELL B CELL C1 C5 CD 2 Pan T cell; (+) Sheep RBCs CD 2 CD 19 C2 Lupus-like syndrome C6 Neisseria C4 C7 infections CD 3 Mature T cell CD 3 CD 20 C3 Severe recurrent infections; C8 CD 4 T helper cell CD 4 CD 21 Glumerulonephritis CD 8 T cytotoxic cell CD 5 CD 22 C9 No known disease associated C1NH Hereditary CD19 Pan B cell CD 7 CD 23 Angioneurotic CD 25 Activated T cell CD 8 Edema
CLASSICAL TRANSPLANT ANTIGENS GEL TEST GRADING REAGENTS IN VDRL HLA-A A 4+ 0.03% cardiolipin Isolated from beef heart; for reactivity HLA-B B 3+ 0.9% cholesterol Center for absorption of tissue lipids to increase HLA-C2 C 2+ size of antigen HLA-DR D 1+ 0.21% lecithin Produce standard reactivity HLA-DQ E 0
DRB, RMT 2019 1
INTERPRETATION ENZYME (EIA) SOURCES AGGLUTINATION INHIBITION ACHe Electrophorous electicus (+) absence of agglutination ALP E. coli (-) presence of agglutination Beta-galactosidase E. coli SINGLE LINEAR IMMUNODIFFUSION Glucose oxidase A. niger (+) precipitin band G6PD Leuconostoc mesenteroides SINGLE RADIAL IMMUNODIFFUSION Lysozymes Egg White (+) precipitin rings Malate dehydrogenase Pig Heart DOUBLE IMMUNODIFFUSION Peroxidase Horseraddish Identity ∩ Non-Identity × 1 RBC unit 1 g/dl Hgb Partial = 1 platelet pooled unit 5,000 to 10, 000/ul MAJOR crossmatch (PS-DR) Patient Serum, Donor Red Cell ONE STAGE ELECTROPHORESIS MINOR crossmatch (PR-DS) Patient Red cell, Donor Serum Rocket/ Laurell (+) Precipitin Rockets (bullet-shaped) Counterimmunodiffusion (+) Precipitin Lines BLOOD COMPONENTS Light spin, then hard spin PRP TWO STAGE ELECTROPHORESIS Hard spin, then light spin FFP Classic (+) Precipitin Arc Immunofixation (+) Precipitin Bands FFP (thawed) 24 hrs Crossed/ 2D (+) Precipitin Rockets Cryoprecipitate (thawed) 6 hrs Pooled unit (thawed) 4 hrs COMPLEMENT FIXATION (+) absence of hemolysis NOMENCLATURE (-) presence of hemolysis CD R1 Rh0 D ASO TITRATION DE R2 rh’ C (+) no hemolysis cde rr rh’’ E (-) hemolysis D R0 hr’ c hr’’ e
DRB, RMT 2019 2
AB neg px during transfusion Allowable amount of blood to be drawn in ml RBC A- B- AB- O- Whole blood AB- only J' C1 )-+ × 450 @) = Plasma AB+ (no prior transfusion) 110 )-+.
NUMBER OF H ANTIGENS Amount of Anticoagulant needed
O > A2 > B > A2B > A1 > A1B ())/J(-)& (@' '/ -& */))&*'&2 = × 14 100 BLOOD BAG COLORS (RA 1517) International FDA Amount of Anticoagulant to be removed A blue Yellow B yellow Pink = 63 @) − MG 1&&2&2 AB pink White O white Blue