Expectations

You will master how to identify standard methods and techniques used in performing
experiments.
In using this module, you should be able to:
1. know some of the research materials and procedures;
2. compare laboratory techniques and methods; and
3. carry-out tasks designed for the lesson.

LESSON 1
Research Materials and Procedures

Pre-Test

I. Multiple Choice: On a separate sheet of paper, write the letter of the best answer.
1. Which of the following is NOT a laboratory technique and method?
A. microbiological B. chemical C. physical D. none of these
2. All are chemical laboratory techniques and methods, except _____.
A. distillation B. spectrophotometry C. extraction D. filtration
3. Centrifugation process is classified as _____ laboratory technique and method.
A. microbiological B. chemical C. physical D. all of these
4. The technique that separates mixture by passing it in solution or suspension or as a vapor
through a medium in which the components move at different rates.
A. chromatography B. titration C. distillation D. filtration
5. A laboratory technique used to determine the unknown concentration of an identified analyte.
A. distillation B. filtration C. titration D. all of these

II. Modified TRUE or FALSE. On a separate sheet of paper, write TRUE if the underlined word
is correct. Otherwise, change the word to make the statement correct.

__________ 1. Chemical laboratory techniques and methods include spectrophotometry,

extraction, and filtration.
__________ 2. Determining the power of hydrogen amount in a given sample is a
microbiological technique and method in the laboratory.
__________ 3. Laboratory techniques and methods include microbiological, physical, and
chemical.
__________ 4. There are eight physical laboratory techniques and methods presented in this
module.
__________ 5. The laboratory techniques and methods are of great help to a researcher in
doing laboratory experiments.
 Looking Back
The Note-taking (Project Data Logbook and Documenting Investigations)
Recall the process of note-taking. Briefly describe the contents of a project data logbook.

Answer:
____________________________________________________________________________
____________________________________________________________________________
____________________________________________________________________________
____________________________________________________________________________
____________________________________________________________________________

Brief Introduction

In doing research, a researcher must take note of the different research materials and
procedures. Are you aware of these laboratory techniques and methods? How about the proper
setting and handling of equipment in doing an experiment? Finally, do you know how to write
your research methodology?
The syllabus presented below is just a brief summary of the lessons for the third quarter.
For now at least you have an idea or bird’s eye view of all the lessons to be tackled for this
grading.

RESEARCH MATERIALS AND PROCEDURES

1. Laboratory Techniques and Methods

A. Microbiological Techniques
B. Chemical Techniques
1. Spectrophotometry
2. Extraction
3. Titration
C. Physical Techniques
1. Weighing
2. Grinding
3. Drying
4. Centrifugation
5. Distillation
6. Filtration
7. Chromatography
8. pH measurement
D. Ethical Issues
2. Setting and Handling of Equipment
3. Writing the Methodology

Activities

Activity 1. Laboratory Techniques and Methods

On a separate sheet of paper, make a table, (see sample below) enumerate at least
three examples of microbiological, physical, and chemical laboratory techniques and methods.
Then, write at least three ethical issues on each technique/ method.

LABORATORY TECHNIQUES AND METHODS

MICROBIOLOGICAL CHEMICAL PHYSICAL
1. 1. 1.
2. 2. 2.
3. 3. 3.
ETHICAL ISSUES
1. 1. 1.
2. 2. 2.
3. 3. 3.

Activity 2. Simple Research.

Instruction. From your enumerated answers in Activity 1, research, and write at least five
steps/ procedures/ equipment used on how to undergo such processes on a separate sheet of
paper following the given format below.
STEPS OR PROCEDURES OR EQUIPMENT USED
1.
A.
M B.
I C.
C D.
R E.
O 2.
B A.
I B.
O C.
L D.
O E.
G 3.
I A.
C B.
A C.
L D.
E.
1.
 A.
B.
C.
C D.
H E.
E 2.
M A.
I B.
C C.
A D.
L E.
3.
A.
B.
C.
D.
E.
1.
A.
B.
C.
D.
P E.
H 2.
Y A.
S B.
I C.
C D.
A E.
L 3.
A.
B.
C.
D.
E.

Activity 3. Follow Through.

Instruction. From your enumerated answers in Activity 1, briefly define your examples on
microbiological, chemical, and physical laboratory techniques and methods.

Remember
  In doing research, a researcher must be familiar with the research materials and
procedures which include: laboratory techniques and methods, setting and handling
of equipment, and writing the methodology.

 The laboratory techniques and methods involved in this module are: microbiological,
chemical, physical, and their ethical issues.
 The examples of chemical laboratory techniques are spectrophotometry, extraction,
and titration. While weighing, grinding, drying, centrifugation, distillation, filtration,
chromatography, and pH measurement are examples of physical laboratory
techniques.

Checking Your Understanding

Guide Questions:
1. Briefly compare microbiological, chemical, and physical laboratory techniques and methods.
____________________________________________________________________________
____________________________________________________________________________
____________________________________________________________________________

2. Enumerate at least three ways on how to set and handle equipment in the laboratory.
____________________________________________________________________________
____________________________________________________________________________
____________________________________________________________________________

3. As a researcher, how are you going to write a research methodology?

____________________________________________________________________________
____________________________________________________________________________
____________________________________________________________________________

Post-Test

Enumeration. On a separate sheet of paper, list down what is being asked.

1. Ways on how to set and handle equipment. (5)
2. Examples of research methodology. (5)
References
Curriculum Guide for Research 1 - 4 (Additional Subjects). October 10, 2014 edition. Republic
of the Philippines, Department of Education, DepEd Complex, Meralco Avenue, Pasig City.