DNA BEPLICA TION

Delinotcoo Tho procoz b wbich PNA okez uoo idorhco

eDpie ol ctaol i alloo DNA COplicalisn.

Conidereonq ooda el conaorwoluon a Parcarta rarols

o DNA, tolicaorn ro ho al 3moA
SerCongervatuve
n orpercvatiuo. oode e trA rCaplicalisn he aodaughler DA
Gemc

olaslor oraed at e rd o tre cpcaton. Cach ol herm Conazt o

cne old ircand ene r o u -canol .

T A
5

old Neo Ne old

Cengoruiodeye
In Conervatwe rmade ol DNA rCeicattor), tuo odaugbker tNA
o Dalaz orsmed atho end o rceicacon, bne daunlerr DNA oesa
CCropheAd Congi-a ottwo olcd gitanda uw hore a arbthoc Conra
u9o nOuo 2 tcand
B
A

'5
cDipergive Tn dgeupleuo rnade of pNA Copliadion, alor
PeTaraiuon of Tacomal zirars, e eqr He al oaeb grand i
ROtooouo di pteol, o a reult cach zlcand toongikl o b»lh
d ord rouo NA 2ltands
A
CE
A
6/
*Areng three -erox ol tDNA coplica}co h e zormiconaorvatio
ode el DNA Keplico-tcon hog univorphf actoptod
Mechankm HaroTtellExlep 1rocopa and -lakoa oluing os
o Coroero
CMeHêng ef TNIA at oceqto
Repiotien o DNA hogeng al aotltc 3ilke colod orcQio H
T ap. 45bp a DNA
Crgn ie ig ATich ond conain Bho rcemeatmotls

anded tepcatco ancd rotOr ACqueroR OKO rceuorAO -tasdoso

tetmted
e O acr A2querto a the bending a e ort o tcoBein caiod
DNa A AAo bdenq ef i z trokein uROR oonderq c-e ora
e recre rcgen ha doradaccd,hch canaeg retnq elp
t
rLn brookenq H benda boldcon cocKegpondarq boaega
raA
qmers
Oric ATP +DraA

13mens ZZZ

Uoonding efNA
DNA Osicateon o tes tha a doublo rrelical poKcntal
rooloulo iz toruOourol - o l H nercl basez ane epos
o e rcepliotbn onArooa
koen Dre C ceesCuet oe Dna B. Dna B a l aa houcare ond
in turo ndung -te DNA
rael
T r o unuoinding roo Crudt n-brroauorn o TIorcCoels chich
PC then rooood t e acleon ol arthor enrsrne Knaun aa
DA OOe (poizooOsOAOR)
Onoindinq oP A oloude zeaKaloz duo xrards do sorem
y-Shaped colicoison -ack
callad SSBS
SBs
The engte aranok arce hound to lihe cotein
c r re relia dealabliaing
CCoa Sfrandled bdingN Trcoleana) e diped
TrOUentakorm celocrnq
prcokeinR Thug 1DNA 1
 |3

ODnac

na B

(a) RNAPoimino o
3
h e eDaOac Drna G ad a PurnaRe and ayothorino ahor
RNA tands oc RNA prtirmera on cnale glranded DNIA
he aucrag Lengto he RNA poroo 10-12 ruclectdez
eracr prtoridle 3-OH arcoup orc cetboaig e DNA DNA D.
A 2nale_RhA pciroor. PDhorized oh DNA 2/rrand ratnq
larcH -5 and m pirmer en tNA 231and hovcrq
olartit 5-3
CG)Soexik of DNA
The-ercrolin af tNA Laker plao by aolduton cl deo ruco
kdo CAIPGTP, CTP ard TTP) to 4re end o RNA
preermor y ne
RepicoluoO af tNA en 35' aHcand and on 5- arno cccura
difforcertuy ond udex alouenq too roccolure
Leadine rond sypthesis
The DNA 3rand wh nharizeo in 5-3diroton on 3-5
teoTa ar ono pieca, kkow ox ioding arcandl
Loaging srond syotheis
h e DNA g-rcands whioh ayotheaized in 5-3 direeibn an 3-5
oreploke a arord aogroentz, krotrn a lag9nq gttand.and the
Neaoorg oxe catlod okoiaki s00morda
fwerago Lonoth e okojak 2qroont ia toco-O00 ruclootohoA
( Caisiorn of RNA
>
Pumers
ccro pleko DNA rcolicalson he RA TtcroeK murt to be
rcovouec and roloud oth DNA
The RNA TIGoor aco rcerno
ued corm tbord one b ono b
-to ator) ef 5-3 eacnueleae
oelivetse of tnA polemo raro I.
 3

Dna B holiage

Ropliato Remase ( Dna G)

fork
DNA
Pol IL
SSB

okojaki
frtoet

Loagina
leaduna ntasig
shand ythe CDiecontnoua)
Csotnous)

HeuoR

Conp-TI Cord-D
po
Eplamp
Jonna of kajaki eqmont
Fenally-he odjaten 5and aond ol aajok goqrmonBz aro joined
DNA lqaao to oro a Coniinou TNA 2lrcond

DNA po: T rromoves

RNA primorg

3
DNA gage joins
okajaki qoet

-5

)Pceol rcading
Cerrodene rcong bago Crte added or nHeoduted
durceng rceplicoon
The koquory in 10.000 The ortonoaRe te'zoo
and caploced uth amoprioo baxe b proe rcodira
aeeveta @f phApodyromercose I C kombercq Come)
lorcroonatcon -
at korcroination atex
Torroinattorn of DNA roplicokon ocur

1 4 oquire produel of tus qone (us kokein o orc ordin9

pcotoin, TBP) wich roeean03 kor c a
Coli, teo ocminäo0 2itox tor A to
h cokorROker Lo E oro

exF cooh conpiiino o23bp

AAho tor t e TBP bind and aiop ho rcoRA o coplicator
ork hbitinq ho Dna B holaro .

n ooding ircand coplicacon -orcninatas al tor aitex

hece og n-ho logainq Arcand H RtoTeo 50-100bp
boforo he tor t e