Discussion

In the API 20E, the plastic strip holds twenty mini-test chambers containing dehydrated media
having chemically defined compositions for each test. They usually detect enzymatic activity,
mostly related to the fermentation of carbohydrates or the catabolism of proteins or amino
acids by the inoculated organisms. A bacterial suspension is used to rehydrate each of the
wells and the strips are incubated. During incubation, metabolism produces color changes that
are either spontaneous or revealed by adding reagents. All positive and negative test results are
compiled to obtain a profile number, which is then compared with profile numbers in a
commercial codebook (or online) to determine the identification of the bacterial species. For
some of the compartments, the color change can be read straightway after 24 hours but for
some reagents must be added to them before interpreting the API. They were observed as
negative therefore no other reagents were added Reading Scale was calculated by marking
positive or negative on the lid of the tray. The wells are marked off into triplets by black
triangles, for which scores are allocated into seven digits number

The data that we acquired from our lab results led us to be able to determine the blood
type of the sample we were given. For the sample, there was agglutination in Anti-A and Anti-
B but no agglutination in Rh which meant that A and B were antigens and Rh was an antibody
which led us to determine that the sample was AB- blood.
After the conclusion of this experiment, it was easier to understand what agglutination
is and how it is important in determining different blood types. It also made it easier to
understand that when agglutination occurs in Anti-A, Anti-B, or Rh that means that it is an
antigen and is on the surface of the red blood cell. If no agglutination occurs in Anti-A, Anti-
B, or Rh then that means that it is an antibody which is a protein found in the plasma. Once we
were able to determine what the antibodies and antigens were from the experiment, we were
able to easily identify what blood type it was. In any experiment, there could always be a
problem that could occur which would alter the results. In this experiment, there were a few
ways that the results could have been altered. One way the results could have been altered was
whether or not the person responsible for stirring the blood and serum stirred too hard or too
fast. Another way the results could have been altered is if there was cross-contamination with
the toothpicks that were used. Our group was able to identify what the sample’s blood type
was which meant it was highly unlikely that anything affected or altered our conclusions about
the blood types.

In the experiment, the microorganisms were able to grow. The microorganisms were able to
grow in the YMA, NA, and CBA, the sample was taken from the capsule. Now we can
identify the microorganisms in the tablet. The experiment is easy but long because you must
wait for the microorganism to grow and do the simple stain. Results may differ from what you
expected due to not doing well during simple stains.