Laboratory Diagnosis

Specimen Collection and Transport

 No special considerations are required for isolation of these genera from extraintestinal sources.
 Stool specimens suspected of containing Vibrio spp. should be collected and transported in Cary-
Blair medium.
 Buffered glycerol saline is not acceptable, because glycerol is toxic to vibrios.

Direct Microscopic Examination

 dark-field microscopy is used

o stool specimens from patients with cholera, the bacilli exhibit characteristic rapid
darting or shooting-star motility.

Reference:

Tille, P. M. (2017). Bailey & Scott’s Diagnostic Microbiology (14th ed.). Mosby.