28/09/2022 3:32 PM Chromatogram E:\HPLC DATA\hplc\28.09.22\Data\Aceclofenac std 3.

PRM Page 1 of 2

BELSEN PHARMACEUTICALS LTD.

KAMLAPUR, FARIDPUR
BANGLADESH
Chromatogram Info:
File Name : E:\HPLC DATA\hplc\28.09.22\Data\Aceclofenac std 3.PRM File Created : 28/09/2022 9:33:19 AM
Origin : Acquired, Acquisition started 28/09/2022 10:13:17 AM Acquired Date : 28/09/2022 9:41:45 AM
Project : C:\YLClarity\Projects\Work1.PRJ By : Administrator

Printed Version Info:

Printed Version : 28/09/2022 3:31:42 PM Recent (Linked Calibration) Printed Date : 28/09/2022 4:15:27 PM
Report Style : C:\YLClarity\Common\Chromatogram.sty By : Administrator
Calibration File : E:\HPLC DATA\hplc\28.09.22\Calibration\Aceclofenac1.CAL

Sample Info:
Sample ID : Aceclofenac std Amount [uL] : 1
Sample : Aceclofenac std ISTD Amount : 1
Inj. Volume [mL] : 20 Dilution : 1

Method : E:\HPLC By : Administrator

DATA\hplc\28.09.22\Method\Aceclofenac

Description : Aceclofenac std

Created : 28/09/2022 10:48 AM Modified : 28/09/2022 10:55 AM

Column : 250x4.6 mm Detection : 270

Mobile Phase : Phosphate Buffer:Methanol (40:60) Temperature : 40
Flow Rate : 1.0 Pressure : 4000
Note :

Autostop : 8.00 min External Start : Start - Restart, Down

Detector 1 : Channel 1 Range 1 : Bipolar, 10000 mV, 10 Samp. per Sec.
Subtraction Chromatogram : (None) Matching : No Change

Base : Area Calibration File : Aceclofenac STD 1 Calculation : ESTD

Scale Factor : Not Used Units After Scaling : Not Used Uncal. Response : 0
Unretained Time : 0.00 min Column Length : 50.00 mm Column Calc. : From Width at 50% of Height
Result Table Reports : All Peaks Hide ISTD Peak : Enabled

[mV] [%]
100
E: \ HPLC DATA\ hplc\ 28.09.22\ Data\ Aceclofenac std 2- Channel 1
120
E: \ HPLC DATA\ hplc\ 28.09.22\ Data\ Aceclofenac std 2- Channel 2
E:\ HPLC DATA\ hplc\ 28.09.22\ Data \ Aceclofenac std3 - Channel 1
100 E: \ HPLC DATA\ hplc\ 28.09.22\ Data\ Aceclofenac std 3- Channel 2 80
E:\ HPLC DATA\ hplc\ 28.09.22\ Data \ Aceclofenac std4 - Channel 1
E: \ HPLC DATA\ hplc\ 28.09.22\ Data\ Aceclofenac std4- Channel 2
80
60
Voltage

Aceclofenac

60

40
40

20
20

0
0 2 4 6 8
[min.]
Time
28/09/2022 3:32 PM Chromatogram E:\HPLC DATA\hplc\28.09.22\Data\Aceclofenac std 3 .PRM Page 2 of 2

Result Table (ESTD - E:\HPLC DATA\hplc\28.09.22\Data\Aceclofenac std 3- Channel 1

Reten. Time Response Amount Peak Compound Name

[min] [mg] Type
1 1.742 17133.523 50.400 Ordnr Aceclofenac std
2 1.737 17112.241 50.400 Ordnr Aceclofenac std
3 1.747 17075.626 50.400 Ordnr Aceclofenac std
Total 50.400

SST Parameters Table

- SST Result for Component " Aceclofenac std ", Calculate By : "EP"
Calculated by Mean Value

X SST Chromatogram Retention Area Height Amount Efficiency

Time [mV.s] [mV] /Length
Lower Limit
Upper Limit
%RSD Limit
Mean 1.742 17107.130 121.946 50.400 11565.397
RSD [%] 0.87 0.89 0.83 0.00 1.43
Parameter Result
E:\HPLC DATA \28.09.22 1.742 17133.523 121.611 50.400 11554.342
E:\HPLC DATA \28.09.22 1.737 17112.241 121.343 50.400 11478.421
E:\HPLC DATA \28.09.22 1.747 17075.626 122.885 50.400 11663.428

Tested by :