REVIEW OF RELATED LITERATURE
‘Soy sauce is a dark brown liquid used in cooking
and preserving food. Fermentation is the process used in
its production. Fermentation is an energy-yielding
metabolic process that involves the: breaking down of
carbohydrates in the absence of oxygen. Fermentation is
also used in the preparation of other food products, such
as wines, beers, aleohol and glycerol.
Avnumiber of studies have been conducted totest the
feasibility of other varieties of beans as substitute
ingredient in soy sauce production. One of those tested
was the winged bean (Psophocarpus tetragonalobus)
Studies showed that winged beans increased the protein
content of soy sauce, However, sensory evaluation tests
revealed a significant preference for the commercial soy
sauce interms of taste, color, odor, texture and acceptability.
‘There is also lower protein content in the winged bean
savee.
METHODOLOGY
PREPARATION OF MATERIALS
For the mold koji, 125 grams of steamed rice was
placed in a 250 millilitres (mL) Erlenmeyer flask and was
autoclaved at 15 psi for 30 minutes. Once cooled, it was
seeded with Aspergillus oryzae. Then, it was left to
incubate for five (5)days.
For the bacterial koji, 50 millilitres of kidney bean
broth was placed in a 250-millilitre flask and was
autoclaved at 15 psi for 30 minutes. After cooling, it was
inoculated with Lactobacillus delbrueckii. Incubation
lasted ffor five (5) days.
Each koji was replicated fortwo batches.of kidney
sauce.
‘Meanwhile, $00 grams of red kidney beans wal
soaked overnight. In the morning, the beans were bell
until tender, The cocked beans were mashed and cooked,
‘Then, 125 grams of wheat flour was toasted on a frying)
pan until pale brown. |
INOCULATION OF CULTURE
‘The mashed kidney beans were mixed with the wheat
flour, The bacterial koji and the mold koji were spread
into the mixture. For five (5) days, the container was
covered with cotton or cheesecloth to let air in and the
contaminants out.
FERMENTATION
‘An 18 percent brine solution was prepared in S00,
tres of water. The solution was boiled without
letting any vapor escape. The solution was then cooled
Tnaclean container, the incubated kidney bean mash
‘was mixed with the brine solution. The container was
tightly closed and was set aside for three (3) months to
allow anaerobic fermentation.
HARVESTINGAND EVALUATION
‘After three (3) months, the two batches of kidney
bean mash were harvested. Then, pasteurization was done
at 60 degrees Celsius for 30 minutes. Caramel, sugar,
salt and pepper were added.
‘Three samples were prepared. Sample.A contained
pure kidney bean sauce. Sample B contained 50 percent
kidney bean sauce and 50 percent soy sauce. Sample C
contained pure soy sauce.
After testing the samples, questionnaires were
accomplished to help evaluate the taste, odor, texture and
acceptability, Friedmann’s Test was also conducted to
HEED BATO BALAN! senior