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Purpose: This study evaluated the effect of application technique and preparation size on the fracture strength
(FS), microtensile bond strength (μTBS) and marginal integrity (MI) of direct resin composite restorations.
Materials and Methods: Conservative (5 × 2 × 2 mm) or extended (5 × 4 × 2 mm) preparations below the
cementoenamel junction were performed in 140 human maxillary premolars (n = 70 per group). After adhesive ap-
plication (XP Bond), half of each group was restored with the bulk technique (one 4-mm increment of Surefill SDR
Flow plus one 1-mm horizontal capping layer of TPH3 [Spectrum TPH3 resin composite]) and half incrementally
(TPH3 in three horizontal incremental layers, 1.5 to 2 mm each), all using a metal matrix band. After storage (24 h
at 37°C), the proximal surfaces of each tooth were polished with Sof-Lex disks. For FS measurement, 60 restor-
ations were mounted in a universal testing machine and subjected to a compressive axial load applied parallel to
the long axis of the tooth, running at a crosshead speed of 0.5 mm/min. For μTBS testing, 40 teeth were longitudi-
nally sectioned to obtain resin-dentin bonded sticks from the cavity floor (bonded area: 0.8 mm2). Specimens were
tested in tension at 0.5 mm/min. The external marginal integrity of both proximal surfaces was analyzed using
SEM of epoxy resin replicas. The μTBS, marginal integrity, and fracture resistance data were subjected to two-way
ANOVA, and Tukey’s post-hoc test was used for pair-wise comparisons (_ = 0.05).
Results: Fracture resistance, microtensile bond strength, and marginal integrity values were not statistically signifi-
cantly affected by application technique or preparation size (p = 0.71, p = 0.82, and p = 0.77, respectively).
Conclusions: The use of a bulk-fill flowable composite associated with a conventional resin composite as a final
capping layer did not jeopardize the fracture strength, bond strength to dentin, or marginal integrity of posterior res-
torations.
Keywords: filling technique, preparation, microtensile bond strength, marginal integrity, fracture strength, direct
resin composite restorations.
J Adhes Dent 2016; 18: 317–323. Submitted for publication: 01.03.16; accepted for publication: 12.05.16
doi: 10.3290/j.jad.a36516
a MSc Student, Postgraduate Program in Integrated Dental Science, Faculty of f PhD Student, Department of Restorative Dentistry, School of Dentistry, State
Dentistry, University of Cuiaba, Cuiabá, Mato Grosso, Brazil. Performed the ex- University of Ponta Grossa, Ponta Grossa, Paraná, Brazil. Performed the ex-
periments, wrote the manuscript. periments, wrote the manuscript.
b MSc Student, Postgraduate Program in Dentistry, Faculty of Dentistry, CEUMA g Professor, Department of Restorative Dentistry, School of Dentistry, State Uni-
University, Sao Luis, Maranhao, Brazil. Performed the experiments, wrote the versity of Ponta Grossa, Ponta Grossa, Paraná, Brazil. Idea, hypothesis, exper-
manuscript. imental design and proofread the manuscript.
c Professor, Postgraduate Program in Integrated Dental Science, Faculty of h Professor, Postgraduate Program in Dentistry, Faculty of Dentistry, CEUMA
Dentistry, University of Cuiaba, Cuiabá, Mato Grosso, Brazil. Performed the ex- University, Sao Luis, Maranhao, Brazil. Idea, experimental design, performed
periments, consulted on and performed statistical evaluation. the experiments and statistical evaluation, wrote the manuscript.
d Assistant Professor, Department of Restorative Dentistry, Division of Opera-
tive Dentistry, College of Dentistry, Jazan University, Jazan, Saudi Arabia. Hy- Correspondence: Alessandro D. Loguercio, Department of Odontology,
pothesis, performed the fracture analysis and proofread the manuscript. Ponta Grossa State University, Avenida Carlos Cavalcanti 4748, Uvaranas,
Ponta Grossa, Paraná, Brazil 84030-900. Tel: +55-42-3220-3741;
e Professor, Department of Restorative Dentistry, School of Dentistry, State Univer- e-mail: aloguercio@hotmail.com
sity of Ponta Grossa, Ponta Grossa, Paraná, Brazil. Idea, hypothesis, experimental
design, and contributed substantially to discussion, proofread the manuscript.
tion and post-operative sensitivity that may or may not be sis tested was that the bond strength, marginal integrity,
associated with microcracks in the adhesive interface. Im- and fracture strength would not be affected by the applica-
perfect adhesion may lead to marginal gaps, which may re- tion technique or cavity size.
sult in microleakage and marginal staining.10,12
One simple way to overcome these problems when res-
toring cavities with light-curing composites is to place and MATERIALS AND METHODS
light cure the resin composite incrementally, thus assuring
adequate curing depth.13 The incremental layering tech- Tooth Preparation and Experimental Groups
nique is the standard protocol to prevent gap formation due One hundred forty caries-free extracted human maxillary
to polymerization stresses and to keep the resin composite premolars were used. The teeth were collected after the
bonded to the dental tissue.13 patients’ informed consent. The University Ethics Commit-
Unfortunately, the incremental filling technique has some tee approved this study under protocol number
disadvantages.13 This technique requires more attention to 644.119/14. Teeth were disinfected in 0.1% thymol, stored
detail during placement of each layer in extended or deep in distilled water, and used within 3 months after extrac-
cavities, and carries an implicit risk of incorporating impuri- tion. The maximum buccal-palatal width of each tooth was
ties or air bubbles between layers. All of this increases the measured with a digital caliper (Absolute Digimatic, Mitu-
treatment time/chair time, mainly when compared with the toyo; Tokyo, Japan) prior to inclusion. All teeth were indi-
bulk-filling technique.1,29 In light of this, some manufactur- vidually mounted in a polyvinyl chloride (PVC) ring filled with
ers have introduced resin composites for specific use with acrylic resin (Aut Clear, DentBras; Pirassununga, SP, Brazil)
the bulk-filling technique. Manufacturers claim that flowable up to 1.0 mm below the cementoenamel junction.
resin composite can be placed in bulk up to 4 mm thick- Teeth were then divided into groups according to the
ness.11,14 In contrast to the older generation of bulk-filling combination of the main factors application technique (bulk
resin composites,31,39 the latest generation has an in- and incremental) and cavity size (conservative and ex-
creased depth of cure when applied up to 4 mm.11,14 Lep- tended). As several properties were evaluated, the number
rince et al32 claimed that the main advancement in the of teeth assigned to each property and group will be de-
depth of cure in this most recent generation of bulk-fill ma- scribed in their respective sections.
terials results from their higher translucency;28 their low
shrinkage stress is related to modifications in the filler con- Restorative Procedure
tent and/or the organic matrix.21 When compared to con- Standardized class II cavities were prepared in all teeth.
ventional, medium-viscosity resin composites, these modi- The occlusal box of these preparations was 5 mm deep,
fications resulted in reduced mechanical properties.7,21,22 and the mesio-distal length at the bottom of the proximal
Based on a literature review, Leprince et al32 reported that box was 3 mm. The proximal box (mesially and distally) was
the elastic modulus of dentin ranges from 12 to 20 GPa,26 6 mm deep with margins located 1 mm below the cemento-
and the newest flowable composites, such as Surefil SDR enamel junction. The internal walls of each cavity were per-
Flow, present lower values (ca. 4 GPa).7,21,22 pendicular to the top and bottom surfaces, with round an-
Due to the reduced mechanical properties of the bulk-fill gles defined by the bur’s shape.
flowable composites, some authors have recommended the At this stage, teeth were divided into two groups accord-
coverage of the bulk-fill materials with an increment of me- ing to the buccal-lingual width. In 20 teeth, the cavity was
dium-viscosity resin composite.21,42 However, it is not clear conservative (2 mm wide in the buccal-lingual direction),
how these reduced mechanical properties can affect the while in the other half the cavity was extended (4 mm wide
fracture strength of large mesio-occlusal-distal (MOD) res- in the buccal-lingual direction). The cavities were prepared
torations. Previous studies that evaluated fracture strength using a diamond bur under water cooling (#4103, KG So-
of restorations using flowable bulk-fill composites only ap- rensen; Barueri, SP, Brazil) and the margins were not bev-
plied them in thin layers or as a sealer.19,38 To the extent elled.
of our knowledge, the fracture strength of restorations In all cavities, the two-step etch-and-rinse adhesive XP
made with flowable bulk-fill composites and an added cap- Bond (Dentsply; Konstanz, Germany) was applied according
ping layer of regular resin composite has not been evalu- to manufacturer’s instructions (Table 1), and they were light
ated so far. cured with an LED light for 20 s at 1200 W/cm2 (Radii-cal,
Although these flowable bulk-fill materials were developed SDI; Bayswater, Victoria, Australia). After adhesive applica-
to achieve better sealing of the cavity margins,5 controversial tion, a metal matrix band was placed and the teeth were
results in terms of marginal properties, marginal leakage, either restored with a bulk-fill material (Surefill SDR Flow
marginal integrity or gaps have been reported in the litera- resin composite, Dentsply) or with a conventional, medium-
ture.2,6,16,36,40 This controversy also extends to the resin- viscosity resin composite (Spectrum TPH3, Dentsply).
dentin bond strength to the dental substrate.15,24,25,45 The flowable composite Surefill SDR was applied in a
Therefore, the objective of this in vitro study was to com- 3.5- to 4-mm layer and then light cured. Subsequently, the
pare the effect of application technique (bulk vs incremen- conventional composite was placed in a 1- to 1.5-mm-thick
tal filling) and cavity size on the resin-dentin bond strength, horizontal layer. Each increment was separately light cured
marginal integrity, and fracture strength. The null hypothe- for 20 s each with the light source in contact with the coro-
Table 1 Adhesive system (manufacturer), composition (batch number), and mode of application
nal edge of the matrix band. After 24 h in distilled water at the digital caliper to the nearest 0.01 mm and recorded for
37°C, the proximal margins of all restored teeth were fin- subsequent calculation of the μTBS (Absolute Digimatic,
ished with flexible disks (SofLex Pop-on, 3M ESPE; St Paul, Mitutoyo).
MN, USA). A single operator carried out all bonding and re- Each stick was attached to a modified device for μTBS
storative procedures in an environment with controlled tem- testing with cyanoacrylate resin (Super Bonder, Loctite; São
perature and humidity. Paulo, SP, Brazil) and subjected to a tensile force in a uni-
versal testing machine (Kratos; São Paulo, SP, Brazil) at a
Fracture Strength crosshead speed of 0.5 mm/min. The failure mode was
Sixty restorations (n = 15 teeth per experimental condition) evaluated at 40X (HMV-2, Shimadzu; Tokyo, Japan) and
were mounted in a universal testing machine and subjected classified as cohesive in dentin (failure exclusively within
to a compressive axial load applied to the center of the oc- dentin, CD); cohesive in resin (failure exclusively within
clusal surface and parallel to the long axis of the tooth and resin, CR); adhesive (failure at the resin/dentin interface,
the slopes of the cusps (rather than the restoration), by A), or mixed (failure at the resin/dentin interface that in-
means of a round-end steel device (8.0 mm in diameter) at cluded cohesive failure of the neighboring substrates, M).
a crosshead speed of 0.5 mm/min. The compressive force
was applied until the specimen fractured and the machine Marginal Integrity
automatically stopped operating. The load required to frac- Impressions of mesial and distal surfaces of 40 restor-
ture the specimens was expressed in Newtons (N). The fail- ations (n = 10 teeth per experimental condition) were then
ure pattern of each specimen was categorized as reparable taken with a low-viscosity vinyl polysiloxane material (Ex-
when the failure was 2 mm above the cementoenamel junc- press, 3M ESPE). These impressions were used for prepar-
tion and irreparable when the failure occurred 2 mm below ation of replicas in epoxy resin (Epofix, Struers; Rødovre,
the cementoenamel junction. Denmark). Replicas were platinum coated (MED 020, Bal-
Tec; Balzers, Liechtenstein) for analysis in a scanning elec-
Microtensile Bond Strength Testing (μTBS) tron microscope (Stereo Scam/ LEO; Cambridge, UK). For
Forty restorations (n = 10 teeth per experimental condition) quantitative margin evaluation, the adhesive interface was
were longitudinally sectioned in both “x” and “y” directions observed under 400X magnification. On each proximal sur-
across the bonded interface with a diamond saw in a Lab- face of the restoration, the interface was divided into 15
cut 1010 machine (Extec; Enfield, CT, USA) under water areas for conservative preparation and 21 for extended
cooling at 300 rpm, in order to obtain resin-dentin sticks preparation (Fig 1). Each area received a score according to
from the cavity floor with a rectangular cross-sectional area gap presence: 0 = no gaps observed; 1 = presence of at
of approximately 0.8 mm2. The number of premature fail- least one gap/irregularity.17,18 The evaluation was per-
ures per tooth during specimen preparation was recorded. formed by a technician under blinded conditions. The mar-
The cross-sectional area of each stick was measured with ginal integrity was expressed as a percentage of the entire
Table 2 Number and percentage of specimens (%) ac- Table 3 Number and percentage of specimens (%) ac-
cording to fracture pattern mode and means and stan- cording to fracture pattern mode and premature failures
dard deviations of the fracture resistance (N) for each and means and standard deviations of the microtensile
experimental condition* bond strength (μTBS; MPa) for each experimental con-
dition
Conservative/ Conservative/ 42 7
11 (73.4) 4 (26.6) 1086.1 (391.2) A 5 (8.6) 4 (6.9) 23.7 ± 4.2 A
Bulk Bulk (72.4) (12.1)
Extended/ 36 5
11 (73.4) 4 (26.6) 1003.2 (505.2) A Extended/Bulk 2 (4.4) 2 (4.4) 21.8 ± 5.1 A
Bulk (80.0) (11.2)
Conservative/ Conservative/ 45
10 (66.7) 5 (33.3) 1140.4 (447.6) A 0 (0.0) 3 (5.7) 4 (7.8) 24.0 ± 5.7 A
Incremental Incremental (88.5)
Extended/ Extended/ 48 8
12 (80.0) 3 (20.0) 1099.4 (425.8) A 0 (0.0) 2 (4.1) 21.1 ± 5.3 A
Incremental Incremental (79.2) (16.7)
*Similar letters in the fracture strength column indicate statistically simi- A/M: adhesive/mixed fracture mode; CD: cohesive fracture mode in dentin;
lar means (one-way ANOVA and Tukey’s post-hoc test, p > 0.05). CR: cohesive fracture mode in resin; PF: premature failures. Similar letters in
μTBS column indicate statistically similar means (one-way ANOVA and
Tukey’s post-hoc test, p > 0.05).
face, as previously recommended by different authors.9,34 reported in the present study.36,42 Regarding cavity sizes, a
No significant effect of restorative technique on the frac- difference between two different preparation techniques re-
ture strength was observed, even with different cavity lated to fracture resistance was not expected, because a
sizes. significant difference between the evaluated techniques
Although significantly reduced mechanical properties has been found only in the case of pronounced destruction
have been reported for bulk-fill flowable resin composite of the tooth.30,41
when compared with a conventional resin composite,7,21,22 It was assumed that polymerization shrinkage stress
the majority of the in vitro studies did not use a last incre- would impose tensile stress on the adhesive interface at
ment of conventional resin composite. Similar cuspal de- the bottom of the cavity and thus affect the bond strength
flection was observed in two studies which compared the and marginal integrity of restorations. Contrary to previous
incremental technique with a capped bulk-fill technique, as studies that evaluated the resin-dentin bond strength on a
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