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Received: 27 April 2019 Revised: 4 October 2019 Accepted: 5 November 2019

DOI: 10.1111/jfpe.13324

ORIGINAL ARTICLE

Millet milk: A comparative study on the changes in nutritional


quality of dairy and nondairy milks during processing and
malting

Aruna Nair U. K. | Vincent Hema | V. R. Sinija | S. Hariharan


Ministry of Food Processing Industries,
Government of India, Indian Institute of Food Abstract
Processing Technology, Thanjavur, The effect of different processing like pasteurization, chilling, freezing, cooling and
Tamil Nadu, India
hardening on the protein and fat content of millet milk in comparison to that of dairy
Correspondence milk and plant milk such as coconut milk was studied. When compared to dairy milk,
Vincent Hema, Indian Institute of Food
Processing Technology, Ministry of Food the millet milk showed higher energy (383 kcal) and comparable level of carbohy-
Processing Industries, Government of India, drates (78%). While the millet milk has higher crude fiber (0.9%) than dairy milk
Pudukkottai Road, Thanjavur 613005,
Tamil Nadu, India. (0.73), it has lesser fiber content than coconut milk (2.26%). Owing to its mineral con-
Email: mariagorretti@iifpt.edu.in tent, millet milk also exhibited high ash content (0.35%). The low temperature pro-
cesses resulted in a very slight reduction of protein (8.5–7.8%) and fat content
(0.74–0.6%) when compared to that of unprocessed millet milk (9.1%) yet it still
stood much higher than that of dairy milk (2.4–1.8% protein and 1.2–1.1% fat) even
after being processed. There was also an increase in TSS and a decrease in viscosity
with lower temperatures. Malted milk showed an increase in protein and fiber con-
tent whereas an increase in ash content. The malting process proves beneficial since
it results in a product with increased protein and decreased fat levels.

Practical applications
When considering a millet milk as a replacement for dairy milk, the replacement capa-
bility of the millet milk should be studied in order to know in which kind of products
it can be applied. Toward the same purpose, it is essential to study the changes in
nutritional quality through processing at different temperatures. Since nutritional as
well as pH, TSS and viscosity play the major role right from flavor to texture in case
of milk products, they were studied upon mainly in this case.

1 | I N T RO D UC T I O N lactose sugar viz., inability to digest the lactose sugar. The rise in lac-
tose intolerant population highlights the need and scope for seeking
Lactose intolerance, although was considered to be belonging to the alternatives for dairy milk (Vesa, Marteau, & Korpela, 2000).
first world countries, is now a widespread reality, reaching the popula- The nondairy milk alternatives are developed from plant sources and
tion around the globe. More specifically, the condition among infants can be sourced from cereals, legumes, nuts, seeds, pseudo-cereals and so

is most common in Finland, whereas cases of adulthood lactose intol- on (Sethi, Tyagi, & Anurag, 2016). The cereal based milk can include milk
from oats (Bernat, Cháfer, González-Martínez, Rodríguez-García, &
erance is most commonly found among East Asians (Harrington &
Chiralt, 2015; Deswal, Deora, & Mishra, 2014), rice (Massa et al., 2001) or
Mayberry, 2008; He et al., 2008; Heyman, 2006). The condition of
corn (Kolapo & Oladimeji, 2008) while legumes such as soy, peanut, and
lactose intolerance is caused due to genetic deficiency in lactase
chickpea (Isanga & Zhang, 2009; Li, Wei, Wu, Rui, & Dong, 2016) are used
enzyme in certain people. This makes them intolerant toward the
too. Milks from nuts are very common ranging from almond, hazelnut,

J Food Process Eng. 2019;e13324. wileyonlinelibrary.com/journal/jfpe © 2019 Wiley Periodicals, Inc. 1 of 7


https://doi.org/10.1111/jfpe.13324
2 of 7 NAIR U. K. ET AL.

and walnut—coconut and almond being the most common sources compare with the millet milk under the same treatment conditions.
(Belewu & Belewu, 2007). While seeds such as hemp (Vahanvaty, 2009) Malted millet milk was used to study the effect of malting on the qual-
are used for plant milks, the pseudo cereals such as quinoa are also used ity of the millet milk. The millet milk and coconut milk were extracted
as sources for plant milks (Pineli et al., 2015). using conventional methods as described as follows. Millet was
Inspite of all these variety of choices being available, when it comes soaked for 8–12 hr and ground in a colloidal ball mill and filtered using
to applicability in replacing the dairy milk in its various forms, which a filter press to get a clarified liquid which is millet milk. One batch of
involves treatments at low and high temperatures, many of these malted millet milk was also included in the study wherein the germi-
sources fail. Tester and Karkalas (1996) studied the cereal based milks, nated millets (kept for germination for 24 hr at 25–30 C) were used
especially oats to find that they contained starch enough to cause gela- in the place of raw millets. Coconut milk was prepared by shredding
tinization on thermal treatments. This changes the physical properties the coconut kernel using a coconut shredder and grinding it in the col-
of milks significantly which further restricts its use in various nondairy loidal ball mill followed by filtering using a filter press to get the coco-
foods. In the case of Soy milk, although the market has already been nut milk. Raw milk was procured from a local dairy farm at Thanjavur.
established and was among the first to be introduced to the market All the milk samples were subjected to various low and high tem-
of nondairy milks, soy allergy and the off flavor it possesses, poses a perature treatments described as follows. Pasteurization was done in
major problem to its further scopes as a dairy replacement (Fukui, the steam jacketed kettle at 63 C for 30 min. Chilling and freezing were
Tachibana, & Wanezaki, 2002). So any nondairy milk with an acceptable carried out in a refrigerator at 4 C for 4 hr and −5 C to −6 C for 4 hr,
sensory quality and protein content can compete with soy milk in the respectively. Cooling and Hardening were done using individual deep
market for dairy alternatives, because they do not pose the risks of freezers set at −18 C for 4 hr and −28 C for 8 hr, respectively.
allergy or off flavor. The same goes for the milks derived from the nuts The samples after each treatment were analyzed for nutritional
as nut allergy is highly prevalent (Sicherer, Burks, & Sampson, 1998). content (carbohydrates, protein, fat, crude fiber, ash, moisture con-
When these factors are considered, millets stand to be good tent, and energy) according to AOAC methods. pH was measured
source for dairy alternatives. Being high in protein, lesser starch con- using a calibrated pH meter and TSS was measured using digital
tent and of mild flavor, it makes up for the lacuna found in other refractometer. Viscosity was measured using Brookefield viscometer
sources. Millets are widely cultivated, and are known to thrive well with 64S spindle at 100 rpm.
with less maintenance, making it an ideal crop to be used as a dairy
replacement through value addition. Millets in India are cultivated in
3 | RESULTS AND DISCUSSION
29 million hectares annually (Prakasha, Murthy, Prathima, & Meti,
2018). It is also one of the underutilized crops and needs to be diversi-
The processes involved were high temperature process such as pas-
fied through value addition. Millet milk is one such product.
teurization and low temperature processes such as chilling, freezing,
Millet milk is mainly preferred due to its nutritional superiority
cooling, and hardening, all compared against unprocessed dairy and
over other sources of plant milks. It is high in protein, but also low in
nondairy milks. The effect of processing on the nutritional content of
calories (Raajeswari & Nithya, 2016). This makes it an ideal replace-
dairy and nondairy milks have been tabulated in Table 1.
ment for dairy especially in the current scenario where high nutrition
and low calorie foods are preferred. But studies need to be done on
the applicability of the millet milk to different types of food wherein it 3.1 | Nutritional composition of unprocessed millet
is subjected to various kinds of processing at different temperatures. milk in comparison with that of dairy milk and
Most of the flavor compounds are fat soluble and fat–protein interac- coconut milk
tions create a platform for the flavor reactions to take place (Lopez,
The unprocessed millet milk showed 3 times more protein (9.17%)
2005). Moreover, flavor and texture are highly influenced by fat con-
than dairy milk (2.84%) and 4.5 times more protein than coconut milk
tent of the food (Drewnowski, 1997). Protein–fat interactions play a
(2.02%) whereas it showed 1.7 times lesser fat (0.68%) than dairy milk
major role in emulsifying activity hence making them the two major
(1.19%) and 3 times lesser fat than coconut milk (2.26%).
components that influence the processing of milk and milk products
When compared to dairy milk, the millet milk showed higher
(Jost, 1993). Hence, it is necessary to study the changes in nutritional
energy (383 kcal) and comparable level of carbohydrates (78%). While
content during processing. This paper deals with the effect of
the millet milk has higher crude fiber (0.9%) than dairy milk (0.73), it
processing on the nutritional quality and its comparison with other
has lesser fiber content than coconut milk (2.26%). Owing to its min-
plant milk and dairy milk.
eral content, millet milk also exhibited high ash content (0.35%).

2 | MATERIALS AND METHODS


3.2 | Effect of malting and other processing methods
on the nutritional content
The millets were procured from the local markets of Thanjavur. Cow's
milk was used as a dairy source to compare with the millet milk (non- The low temperature processes resulted in a very slight reduction of
dairy milk). Coconut milk was used as a nondairy milk source to protein (8.59–7.8%), and fat content (0.74–0.6%) when compared to
NAIR U. K. ET AL. 3 of 7

TABLE 1 Effect of processing on the nutritional content of the dairy and nondairy milk samples

Processing condition Temperature involved Millet milk Malted millet milk Cow milk Coconut milk
Protein (%)
Unprocessed 32 C 9.17±0.062 9.69±0.033 2.84 ± 0.066 2.02 ± 0.021

Pasteurization 63 C 7.91±0.020 9.14±0.017 1.84 ± 0.014 1.24 ± 0.016
Chilling 4 C 8.59±0.067 8.89±0.028 2.42 ± 0.012 1.68 ± 0.020
Freezing −5 to −6 C 8.19±0.018 8.46±0.017 2.18 ± 0.097 1.615 ± 0.035
Cooling −18 C 7.80±0.017 7.95±0.005 1.84 ± 0.017 1.57 ± 0.015
Hardening −28 to −35 C 7.96±0.008 8.19±0.020 1.97 ± 0.012 1.59 ± 0.007
Fat (%)
Unprocessed 32 C 0.68±0.016 0.6±0.005 1.19 ± 0.012 2.26 ± 0.012
Pasteurization 63 C 0.64±0.009 0.46±0.016 1.14 ± 0.017 1.92 ± 0.020

Chilling 4 C 0.74±0.012 0.62±0.008 1.20 ± 0.014 2.13 ± 0.005
Freezing −5 to −6 C 0.66±0.016 0.58±0.012 1.15 ± 0.022 2.09 ± 0.030

Cooling −18 C 0.63±0.021 0.55±0.021 1.07 ± 0.016 1.86 ± 0.015
Hardening −28 to −35 C 0.60±0.012 0.54±0.012 1.14 ± 0.009 1.94 ± 0.010
Moisture (%)
Unprocessed 32 C 9.733 ± 0.097 10.41 ± 0.188 10.21 ± 0.017 27.61 ± 0.514

Pasteurization 63 C 10.14 ± 0.233 7.4 ± 0.208 12.97 ± 0.286 37.15 ± 0.521
Chilling 4 C 10.31 ± 0.174 6.21 ± 0.292 11.80 ± 0.055 22.07 ± 0.231

Freezing −5 to −6 C 10.90 ± 0.041 7.21 ± 0.051 13.38 ± 0.133 18.25 ± 0.123
Cooling −18 C 9.64 ± 0.160 8.36 ± 0.156 11.86 ± 0.187 21.17 ± 0.382
Hardening −28 to −35 C 8.21 ± 0.049 12.28 ± 0.071 11.66 ± 0.130 22.08 ± 0.170
Ash (%)
Unprocessed 32 C 0.35 ± 0.009 0.05 ± 0.012 0.25 ± 0.024 0.13 ± 0.042
Pasteurization 63 C 0.29 ± 0.005 0.02 ± 0.005 0.33 ± 0.012 0.09 ± 0.005

Chilling 4 C 0.24 ± 0.012 0.003 ± 0.005 0.45 ± 0.020 0.28 ± 0.024
Freezing −5 to −6 C 0.23 ± 0.005 0.006 ± 0.005 0.43 ± 0.022 0.33 ± 0.020

Cooling −18 C 0.23 ± 0.008 0±0 0.45 ± 0.029 0.17 ± 0.017
Hardening −28 to −35 C 0.24 ± 0.016 0±0 0.48 ± 0.031 0.27 ± 0.017
Crude fiber (%)
Unprocessed 32 C 0.9 ± 0.016 0.97 ± 0.055 0.73 ± 0.014 2.26 ± 0.160

Pasteurization 63 C 0.65 ± 0.009 0.69 ± 0.025 0.84 ± 0.012 2.17 ± 0.041
Chilling 4 C 0.86 ± 0.028 1.12 ± 0.054 0.45 ± 0.025 1.99 ± 0.029
Freezing −5 to −6 C 0.33 ± 0.020 0.75 ± 0.024 0.46 ± 0.022 2.07 ± 0.037
Cooling −18 C 0.31 ± 0.054 0.59 ± 0.026 0.23 ± 0.017 1.23 ± 0.093
Hardening −28 to −35 C 0.32 ± 0.065 0.58 ± 0.040 0.26 ± 0.037 1.22 ± 0.074
Carbohydrates (%)
Unprocessed 32 C 78.727 ± 0.015 78.72 ± 0.023 84.78 ± 0.005 65.72 ± 0.005
Pasteurization 63 C 79.32 ± 0.014 83.34 ± 0.061 82.88 ± 0.009 57.43 ± 0.005

Chilling 4 C 79.08 ± 0.076 83.337 ± 0.038 83.68 ± 0.012 71.85 ± 0.064
Freezing −5 to −6 C 79.5 ± 0.026 83.184 ± 0.042 82.4 ± 0.013 75.645 ± 0.042
Cooling −18 C 81.32± 82.62 ± 0.051 84.55 ± 0.024 74 ± 0.022
Hardening −28 to −35 C 82.5± 78.58 ± 0.005 84.49 ± 0.042 72.9 ± 0.009
Energy (kcal)
Unprocessed 32 C 383.108 ± 0.003 380.96 ± 0.028 336.15 ± 0.028 269.02 ± 0.017

Pasteurization 63 C 383.74 ± 0.009 393.04 ± 0.017 322.66 ± 0.022 230.4 ± 0.024
(Continues)
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TABLE 1 (Continued)

Processing condition Temperature involved Millet milk Malted millet milk Cow milk Coconut milk

Chilling 4 C 380.26 ± 0.012 397.208 ± 0.036 331.64 ± 0.069 291.21 ± 0.014
Freezing −5 to −6 C 378.4 ± 0.025 393.516 ± 0.018 325.83 ± 0.006 307.27 ± 0.015

Cooling −18 C 382.31 ± 0.042 389.43 ± 0.021 332.83 ± 0.012 299.02 ± 0.042
Hardening −28 to −35 C 388.42 ± 0.041 373.36 ± 0.024 333.62 ± 0.007 295.3 ± 0.022

Note: Data represented in the table are in the form of mean ± SD, n = 3.

F I G U R E 1 Effect of processing on
the pH of dairy and nondairy milk samples

that of unprocessed millet milk (9.17%) yet it still stood much higher affected by temperature involved processes (but mainly heat shocks)
than that of dairy milk (2.42–1.84% protein and 1.2–1.07% fat) even (Prindiville, Marshall, & Heymann, 1999) backs up the observation that
after being processed. The protein content shows less changes with the fat separation was more in case of millet (malted and unmalted)
each processing because the alteration in temperature is reversible milk during processing because it is a low fat milk. Homogenization is
causing a repeptization upon bringing to normal temperature or recommended in such kind of milks to lower the fat separation.
thawing, in case of hydrophilic sols (Webb & Hall, 1935). But lower Malted milk showed a higher protein content (9.7%) and lower
the temperature, tougher it is for normalizing upon thawing. Hence, fat content (0.6%) when compared to that of unmalted milk. The
the decreasing trend is shown for protein in the current study is justi- increase in protein content can be attributed to the decrease in
fied. In case of fat, it is present as an emulsion with a layer of protein moisture content during the germination process with a subsequent
adsorbed on it. This layer of protein provides a protective effect caus- reduction in total solid loss as it gets transferred to the embryo, as
ing only a partial disruption in the emulsion. But once the protein layer pointed out by Bera, Sabikhi, and Singh (2018). Similar results in
gets disturbed, as discussed above, the emulsion can be accessed and other grains have been shown in the studies of Warle, Riar, Gaikwad,
destructed resulting in the changes in fat content (Doan & Baldwin, and Mane (2015) and Kindiki, Onyango, and Kyalo (2015). A study
1936). Changes in temperature can bring about changes in interaction by Fasasi in 2009 (Fasasi, 2009) inferred that the increase in protein
between fat globules and protein (Lopez, 2005). At the low tempera- content could be due to protein synthesis for the embryo during the
tures during thorough crystallization, the fat content tends to accumu- process, which supports our current results. The increase in protein
late toward the upper layer of the frozen sample. Meanwhile, the could also be because of the stored nitrogen content getting mobi-
protein tends to aggregate toward the middle layer and sugars to lized during the malting process (Gernah, 2011). Crude fiber has
the bottom layer of the mass that is frozen. Now, the fat content in been shown to increase during malting which could be due to the
the milk during this phase is highly influenced by the sampling portion. building up of the dry matter for supplementing the growth of
Moreover, the layers get miscible due to the reconstitution occurring embryo (Laxmi, 2015; Vasantharuba, 2013). The slight decrease in
during thawing, resulting in redistribution in the components during fat could be attributed to the possibility of fatty acid oxidation and
further cooling. This results in an increase or decrease in the fat and lipid hydrolysis during malting (Choudhury, 2011; Lorenz, 1980;
protein percentage (Cvitl, 1931; Iviantoyani, 1933; Koenig, 1930; Maria Victória & Grossmann, 1998). The decrease in ash content can
Munkwitz, By, & Boye, 1933). The fact that low fat foods are more be related to the translocation of nutrients to the growing root and
NAIR U. K. ET AL. 5 of 7

F I G U R E 2 Effect of processing on
the TSS of dairy and nondairy milk
samples

F I G U R E 3 Effect of processing on
the viscosity of dairy and nondairy milk
samples

shoot followed by leaching during processing (Muhammad Arif, There is a slight decrease in the pH followed by increase. This hap-
2011; Sankararao & Deosthale, 1980). This is why malted millet milk pens in milk and the small increase is because the amount of potas-
showed a very low ash content and lost the same during subsequent sium salt that is precipitable was small. pH increases can also be
processing. Hence, malting proves to be beneficial for enhancing the caused due to increase in protein concentration in the phase that
nutritional content of the millet milk. remains unfrozen. This may happen during the thawing stage before
measurement of pH. Figure 2 shows the effect of processing on TSS
of the samples. This can be attributed to the concentration that
3.3 | Effect of processing on the pH, TSS, and
occurs due to crystal formation at low temperatures. Figure 3 shows
viscosity
the effect of processing on the viscosity of the samples. While the
Figure 1 shows the effect of processing on the pH of the samples. It is sudden increase in viscosity during much lower temperatures can be
evident from the graph that on an overall pH remains unaffected. attributed to the concentration process as mentioned in the TSS part,
6 of 7 NAIR U. K. ET AL.

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