BCH4053 – Study Union with Courtney K.

Topic 1: Entropy, enthalpy, and energy

1. For the conversion of reactant A to product Z, the equilibrium constant
of the reaction is 2.
a) Find the standard free energy change for this reaction at 298K

b) What is the free energy change when 5µM of A is mixed with 50µM
of Z?

c) Based on the free energy change under the conditions from part b,
does the reaction favor the product or the reactant?

Topic 2: Water and Acids

2. Find the pH of a 1L solution that contains 10mL of 0.5M acetic acid and
5mL of 2M sodium acetate. The pKa for acetic acid is 4.76
3. Which of the following is true:

4. The following pKa values have been given for arginine:

pKαCOOH = 1.8
pKαNH3 = 9
pKside-chain = 12.48
A. Sketch the structure of arginine present when pH = 4

B. Sketch the structure of arginine present when pH = 11

 C. Sketch the titration curve of arginine (pKαCOOH = 1.8; pKαNH3
= 9; pKside-chain = 12.48)

Topic 3: Sugars
5. What is the systematic
name for the following
sugar, lactose?
Topic 4: Amino acids and Proteins
6.

7. If you are planning to perform an anion-exchange purification of a

protein that has a pI of 7.5, what pH would you want to choose for the
purification?

8. Which of the following be most likely to fold into an alpha helix?

(a) AEASVKMAVDNAART

(b) TEGNFGAPKSIL

(c) TDAGNEKIVETY
9. A protein of an unknown structure was purified. Gel filtration
chromatography reveals that the native protein has a molecular weight of
240 kDa. SDS-PAGE, in the absence of reducing agents, produces a
single band around 60 kDa. When an SDS-PAGE is performed in the
presence of beta-mercaptoethanol (B-ME), two bands near 34 kDa and 26
kDa are observed.
A. What happens to proteins that are treated with SDS and heat?

B. What happens to proteins that are treated with reducing agents?

C. Sketch the protein quaternary structure based on this

information.
Topic 5: Lipids and the Membrane
10. A dysfunctional, mutant version of the Sodium-Potassium ATPase
has been discovered. After investigating, it is found that this mutant is
locked in the E1 conformation and is unable to enter the E2 conformation.
Based on this information, which of the following are true (select all that
apply).
A. The protein can pump sodium ions out of the cell

B. The protein can bind sodium ions

C. The protein can pump potassium ions into the cell

D. The protein can bind potassium ions

E. The protein likely has a mutation in the transmembrane domain,

resulting in this dysfunctional mutation

F. The protein likely has a mutation in the domain responsible for

hydrolyzing ATP to ADP, resulting in this dysfunctional mutation

11. Which of the following transporters would have its activity impaired if
the cell was placed in a basic environment?
A. Sodium-potassium ATPase
B. Calcium ATPase
C. Lactose Permease
D. Aquaporin
Topic 6: Myoglobin and Hemoglobin
12. For a hemoglobin-like molecule with a Hill coefficient of 1 and the
same p50 value as normal hemoglobin, pick the statement below that
best describes the two different proteins.

Topic 9: Kinetics and Inhibition

13. A known enzyme in the blood catalyzes the conversion of CO2 into a
soluble product. We know that the KM of the enzyme is 1.2x10-3, and that
the kcat of the enzyme is equal to 106 s-1.
A reaction is set up where the enzyme concentration is 107 and the
concentration of CO2 is 0.01M.
A. Find the Vmax for the reaction stated above.
 B. What is the initial rate of the reaction under the given conditions?

Topic 10: Bio-signaling

14. The following protein highlighted in yellow is likely a(n):
A. G-protein coupled Receptor (GCPR)
B. Trimeric G-protein
C. Receptor Tyrosine Kinase
D. Ras family protein