Agilent 1100/1200

Liquid Chromatograph

Scope of Work ---- Installation and Familiarization

Scope of Work
Installation and Familiarization for the 1100/1200 Series
Liquid Chromatograph
1. Scope
This document provides details of the procedures necessary for the successful Installation and Familiarization
(I&F) of a 1200 Series LC and the associated recording device. It clarifies expectations and responsibilities and
assures uniform delivery of the service. Only Support Providers trained and certified by Agilent Technologies
will provide the service. Support Providers are Agilent Customer Engineers (CE’s) and/or Agilent
Authorized Support Providers (ASPs). References to other documents are included to supply additional
information or to provide clarity. Delivery time for the completion of the Installation and Familiarization is
dependent upon the type of recording device to be used (ChemStation or Integrator). Time starts when the
Support Provider arrives at the Customer site and ends when he/she leaves the site. Installation and
Familiarization is comprised of the following parts:
Installation Familiarization
1. Setup 1. Familiarization
2. Checkout 2. Service Review
3. Service Review
Note: This document is to be used by Agilent Employees and non-Agilent ASPs, and is not to be provided to
customers.

2. Applicable Models
This document applies to the following models. This document and the associated install and familiarization times
do not apply to the install/configuration of a non-bundled PC or of the GPIB or LAN board or connection of the PC
to an existing LAN. Extra time must be quoted for these configurations.
Table 2-1. Applicable Models
System Component Product Number Description
Degassing G1322A/79A/B Agilent 1100/1200 Series Online Vacuum Degassers for pumps
G1310A Agilent 1100/1200 Series Isocratic Pump
Pumps G1311A Agilent 1100/1200 Series Quaternary Pump
G1312A Agilent 1100/1200 Series Binary Pump
G1376A Agilent 1100/1200 Capillary Pump
G2226A Agilent 1100/1200 Series Nano Pump
G1361A Agilent 1100/1200 Series Isocratic/Binary Preparative Pump
G1328B/5065-9922 Agilent 1100/1200 Series (Prep.)Manual Injector
Sample Introduction G1329A/B Agilent 1100/1200 0 Series Thermostatted Autosampler
G2260A Agilent 1100/1200 Series Preparative Autosampler
G1367A/B/C/D Agilent 1100/1200 Series High Performance Autosampler
G1377A Agilent 1100/1200 Series Micro Well Plate Autosampler
G2258A Agilent 1100/1200 Series Dual Loop Autosampler
G1313A Agilent 1100 Series Dual Loop AutoSampler
G1330B Agilent 1100/1200 Series Thermostat for Autosamplers or Fraction Collectors
Oven/Heater G1316A/B Agilent 1100/1200 Series Thermostatted Column Compartment
Detectors G1314A/B/C/D/E Agilent 1100/1200 Series Variable Wavelength Detector
G1315A/B/C/D Agilent 1100/1200 Series Diode Array Detector
G1321A Agilent 1100/1200 Series Fluorescence Detector
G1362A Agilent 1100/1200 Series Refractive Index Detector

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Agilent 1100/1200
Liquid Chromatograph

Scope of Work ---- Installation and Familiarization

G1365A/B/C/D Agilent 1100/1200 Series Multiple Wavelength Detector

G4218A Agilent 1100/1200 Series Light Scattering Detector
Fraction Collectors G1364B/C/D Agilent 1200 Series Fraction Collectors
Valves G1157A/58A/59A/50A/ Agilent 1200 Series Valves
62A/63A
Control G4208A Agilent 1200 Series Instant Pilot plus CAN cable
Single Instrument LC 2D ChemStation
G1317BA
Includes HP/Compaq PC, Monitor,
With PC/SW
HP LaserJet printer, LC 2D software and Windows XP.
Single Instrument LC 3D ChemStation
G1319BA
BUNDLES Includes HP/Compaq PC, Monitor,
With PC/SW
(I&F time included with purchase HP LaserJet printer, LC 3D software and Windows XP.
and H1172A included with HPLC 2D ChemStation S/W License Bundle
G2215BA
purchase Includes HP/Compaq PC, Monitor, HP LaserJet printer,
With PC/SW LC 2D License SW and Windows XP. Does not include installation.

3. Time Required
Time required is dependent upon the LC modules sold and the type of recording device present. For an 1100/1200
2D LC bundled with ChemStation, typical Installation and Familiarization time is 6.5 hours. Typical time required
for an 1100/1200 3D LC bundled with ChemStation is 8.5 hours.

Table 3.1 Typical time required for I&F of a Standalone 1200 LC Module
Time Model Description
0.5 hour I&F G1379A/B Agilent 1200 Series Vacuum Degasser/Micro Vacuum Degasser
I hour I&F G1322A Agilent 1200 Series Vacuum Degasser/Micro Vacuum Degasser
1 hour I&F G1310A Agilent 1200 Series Isocratic Pump
1 hour I&F G1311A Agilent 1200 Series Quaternary Pump
1 hour I&F G1312A Agilent 1200 Series Binary Pump
1 hour I&F G1376A Agilent 1200 Series Capillary Pump
1 hour I&F G2226A Agilent 1200 Series Nano Pump
1 hour I&F G1361A Agilent 1200 Isocratic/Binary Preparative Pump
0.5 hour I G1328B/5065-
Agilent 1200 Series Manual Injector, preparative scale Manual Injector
9922
1.hour I&F G1329A/B Agilent 1200 Series Thermostatted Autosampler
1 hour I&F G1387A Agilent 1200 Series Micro Autosampler
1 hour I&F G2260A Agilent 1200 Series Preparative Autosampler
1 hour I&F G1367A/B/C/D Agilent 1200 Series High Performance Autosampler
1 hour I&F G1377A Agilent 1200 Series Micro Well Plate Autosampler
1.5 hour I&F G2258A Agilent 1200 Series Dual Loop Autosampler
0.6 hour I&F G1330B Agilent 1200 Series Thermostat for Autosampler or Fraction Collector
0.5 hour I G1316A Agilent 1200 Series Thermostatted Column Compartment
1 hour I&F G1314A/B/C/D/E Agilent 1200 Series Variable Wavelength Detector
1 hour I&F G1315B/C/D Agilent 1200 Series Diode Array Detector
1 hour I&F G1321A Agilent 1200 Series Fluorescence Detector
1 hour I&F G1362A Agilent 1200 Series Refractive Index Detector
1 hour I&F G1365B/C/D Agilent 1200 Series Multiple Wavelength Detector
1.5 hour I&F G4218A Installation of the detector, instrument equilibration, run of test sample, familiarization
1 hour I&F G1364B/C Agilent 1200 Series Fraction Collectors
1.5 hour I&F G1364D Agilent 1200 Series Fraction Collectors
0.5 hour I&F G1157A/58A/ Agilent 1200 Series Valves
59A/60A/62A/
63A
For most current information please refer to http://services-cag.scs.agilent.com/marketing/main.htm

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Agilent 1100/1200
Liquid Chromatograph

Scope of Work ---- Installation and Familiarization

Table 3.2 Typical time required for I&F of a Standalone LC System (with Control Module)
Time Tasks Customer Comments
Present
Approx.3 Pre-install Discussion  A Customer Representative should provide assistance in placement of the LC onto the bench.
hours
(depending
System Setup  The customer should be present due to the short I&F time. This is a good opportunity to
demonstrate to the customer the installation of the tubing, flow cell, and column.
on the
installed Instrument Checkout  LC checkout conditions using the control module.
modules) LC Familiarization  LC Hardware Familiarization Checklists.

Table 3.3 Typical time required for I&F of a 2D/3D LC with ChemStation
Time Tasks Customer Comments
Present
Approx. Pre-install Discussion  A Customer Representative should provide assistance in placement of the LC onto the bench.
6.5hrs System Setup Although the Customer is not required to be present during setup, this is a good opportunity to
(2D-Value demonstrate to the customer the installation of the tubing, flow cell, and column.
System)
Instrument Checkout LC checkout conditions.
or LC Familiarization  LC Hardware Familiarization Checklists
ChemStation Familiarization Checklist and ChemStation Tutorial
8.5 hrs(3D) Familiarization  Additional 2 hour familiarization of Spectral software for 3D system
Maintenance  Instrument and PC
(depending
on the
Service Review 
installed
modules)

Table 3.4 Typical time required for I&F of a G4218A ELSD

Time Tasks Customer Comments
Present
1.5 hours Pre-install Discussion  Check lab requirements according to customer responsibilities
Detector installation  Installation should be explained to the customer, so installation can be done by the customer,
and equilibration if the ELSD is used in a different lab or an instrument exchange is done. Safety considerations
should be discussed here. Equilibration time can be used for introduction in theory of
operation.
Familiarization 
Checkout sample 

Table 3.5 Typical time required for H1172A Image Solution

Time Tasks Customer Comments
Present
1 hour Create Image, H1172 The customer should be present due to the short I&F time. This is a good opportunity to
Backup Solution explain the customer the need of backup solutions and PC maintenance

Table 3.6 Typical time required for Agilent LC Diagnostic

Time Tasks Customer Comments
Present
25 minutes Install LC Diagnostic, For 1200 Series Rapid Resolution SL Hardware Systems:
Establish Connection , The customer should be present due to the short I&F time. This is a good opportunity to
Familiarization demonstrate the use of this tool and the associated documentation.

(10 minutes) For 1200 Series Hardware Systems:

Describe only Describe Guided Diagnostic, Status Report and generate PDF report

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Agilent 1100/1200
Liquid Chromatograph

Scope of Work ---- Installation and Familiarization

4. Customer Responsibilities
In preparation for the Installation, the Call Initiator (see below for a definition of the Call Initiator) verifies with
the Customer that they have complied with the Site Preparation Checklist. Specific Site Preparation
information (dimensions, weights, solvents, environmental, and electrical specifications) can be found in the
1100/1200 Site Preparation Specification.

 Customer should have available HPLC grade Isopropanol, Acetonitrile and Water
 The site is well ventilated and free of corrosive materials and overhanging obstacles.
 Site temperature is within the recommended range of 0 to 55C for the G1314A/B/C/D/E, G1315B/C/D,
G1316A, G1322A, G1365B/C/D, G1362A, 4 to 45C for the G1379B, 4 to 40C for the G1330B, G1361A,
G1364B/C/D, G2258A, 0 to 40C for the G1321A and all other modules 4 to 55C.
 The sites ambient temperature conditions must be stable, 2C/hour or less is required to meet Agilent
Technology’s published drift specification. The greater the temperature fluctuation in the lab the greater
baseline instability of the LC System. Take care in placement of the system, trying to avoid placement near
heating and air conditioning vents to insure optimum performance.
 Site humidity is within the recommended range of <95%, non-condensing.
 Bench space is adequate and can support the weight of the Agilent 1200 system, including all LC modules and
the ChemStation PC. Additionally the instrument requires 2.5 cm (1.0 inch) of space on either side and 8cm
(3.1inch) at the rear for circulation of air and room for electrical connections. The TCC requires 25cm (10
inches) of space on either side.
 An AC outlet is required for each module, in addition to the Computer System.

 The ELS detector is designed for the use with an LC system. A successful installation requires the
availability of a complete HPLC/RRLC system including a solvent supply, pump, sampler, column and data
acquisition/evaluation system (PC with Chemstation, EZChrom etc.). Using a thermostatted column
compartment and degasser is recommended. The ELSD is often installed as a second detector following a
non-destructive detector based on UV/VIS absorption (DAD/VWD/MWD). While a second detector is not
mandatory, it may help demonstrating a successful installation. The detector is controlled via RS232, not
via LAN/CAN, so it must be installed close to the control PC unless special data transmission systems are
used. The length of the included straight female/female RS 232 cable is 1.7 m.
 Customer should have available HPLC grade Acetonitrile and Water with a dry residue below 1 ppm or MS
grade solvents. See next section regarding requirements for a supply of pressurized nitrogen and an exhaust
line.
 The site is well ventilated and free of corrosive materials and overhanging obstacles.
 Site temperature is within the recommended range of 5 to 40°C for the G4218A.
 Site humidity is within the recommended range of <80% for temperatures below 31 °C, non-condensing.
 Bench space is adequate (size H 450 mm x W 250 mm x D 550 mm / H 17.7 in x W 9.8 in x D 21.7 in) and can
support the weight of the detector (20 kg / 45 lb). Additionally the instrument requires 2.5 cm (1.0 inch) of
space on either side of it and 8cm (3.1inch) at the rear for circulation of air and room for electrical connections.
 The detector must be installed close to the LC system in order to ensure good measurement results using short
capillary connections.
 Following connections must be available
 An AC outlet-

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Agilent 1100/1200
Liquid Chromatograph

Scope of Work ---- Installation and Familiarization

Product number(s) Line Voltage Frequency Current Power (or VA)

G4218-64000 230 V +/- 10% 50 Hz 1.7 A 400 W
G4218-64010 115 V +/- 10% 60 Hz 1.8 A 210 W
G4218-64020 100 V +/- 10% 50 or 60 Hz 2.1 A 210 W

5. Agilent Responsibilities
5.1. Scheduling—The Call Initiator

The Installation call begins with a call to the Customer by the “Call Initiator.” The Call Initiator may be the
Support Provider, a workload administrator, or a dispatcher, depending on local processes. The Call
Initiator:
 Calls the Customer, schedules the date and time for I&F, and confirms Customer preparation.
 With the G4281A see below

 NOTE: the availability of pressurized nitrogen and an exhaust line is not typical for LC installations but it
is critical for the installation of the ELSD. The required pressure is 3.5 bar and must be controllable by an
external pressure regulator (which is available optionally as G4218-60100). The nitrogen must not contain
particles (which would create a noisy background) or should be filtered with a filter of 0.5 µm or less (the
optional pressure regulator G4218-60100 uses a 0.01 µm filter). Gas consumption is typically 3 l to 4 l per
minute. The diameter of the tube coming from the G4218A ELSD is 6 mm (0.236 inch). The customer must
provide a connector of appropriate size.
 If the lab is not prepared for the installation, there will be a significant delay for the customer as well as the
support provider. It is a customer responsibility to prepare the lab accordingly, but it is the responsibility of
the call initiator to point to this fact in order to avoid delays and additional costs for both sides.

Reminds Customer not to open/unpack boxes until an Agilent representative is present.
 Discusses possible problem areas before Support Provider goes on site

5.2. Support Provider Responsibilities

All Support Providers shall be Agilent trained and certified to install and familiarize a customer on the 1200
LC. Support Providers shall take necessary Electro-Static Discharge (ESD) precautions (see appropriate
Hardware Manual) when working on or near printed circuit boards or other sensitive assemblies.

5.2.1. Delivery
 Brings all necessary tools for LC system installation including the Isocratic Checkout sample,
part number - 01080-68704 (Service Note 01200-048) and 8500-6917 Caffeine Standard for
ELSD (if applicable for the ELSD)
 Complete all steps in the I&F Checklist that are applicable to the instrument. Note that the
I&F Checklist is a separate document.
 Brings the prep. Checkout column for a Prep. Scale Purification System - 846975-202 (if
applicable)
5.2.2. Documentation

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Agilent 1100/1200
Liquid Chromatograph

Scope of Work ---- Installation and Familiarization

 Asks the Customer to sign the I&F Checklist acknowledging the completion of service.
 Affix all data generated to the Checklist and give to the customer for addition to the instrument
logbook for future reference.

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Agilent 1100/1200
Liquid Chromatograph

Scope of Work ---- Installation and Familiarization

6.Installation and Familiarization Task Details

6.1. Discussion Before Install

 Review with the Customer the information listed in Table 3.1, 3.2, 3.3, making sure that the Customer
will be present for the tasks that require the Customer to be present.
 Arrange to have assistance placing the LC system on the bench.
 Check that the site preparation requirements have been fulfilled and verify where the Customer would
like the 1200 system installed.
 Ask Customer where to store additional consumables, software, and manuals.
 Coordinate with Customer where to place empty boxes and packing material, as the instrument is
unboxed.

6.2. Setup

The following table summarizes the steps involved in setting up the 1200 system. More details can be
found in the Installation section of the Module Reference Manual.

Table 6-2 Setup Details

Step Comments
Unpack  Remove all equipment from the boxes and place on the bench in the location specified by the customer.
Keep the shipping containers until the Checkout has been completed.
 Store the consumables, software, and manuals in the location specified by the customer.
Check for Completion of Shipment and  Check the items for signs of damage. Any damage or missing in shipment should be reported to Agilent
Signs of Damage Technologies Order Fulfillment or follow local processes as determined by field management.
 Check all items received against the packing list. Any discrepancies should be immediately reported to
the Customer and Order Fulfillment.
 Record all Serial Numbers on the Installation Checklist.
Electrical/Plumbing  Install LC Modules in the 2 stack configuration found in the Site Preparation Specification.
 Install CAN cables from module to module. Install LAN the cable from PC to Detector module, if more
than one detector, install LAN cable to 3D detector.
 Install Autosampler – Thermostat cable if applicable. Note-Do not connect power to this module unless
this cable has been installed. Failure to do so can result in damage to the electronics of this module.
 Connect power cables to each module, install remote cable from Degasser Module, to closest available
remote port.
 Connect capillary from module to module as specified in the module Reference Manual.
 Install waste drains routing outlet to waste reservoir. Note- Ensure the outlet of the detector module is
attached to large bore tubing to prevent excessive back-pressure on the flow cell.
 Install Control Module if applicable.
LC ChemStation Install  For LAN-Configure ChemStation, TCP/IP, if necessary install BOOTP Service software, turn on
(if applicable) LC detector to get MAC address, configure BOOTP manager.
 Install the ChemStation (if applicable) using the appropriate ChemStation Installation guide for
reference.
 Establish communication with the 1200 LC.
LC Diagnostic Install  Set up LAN-Connection on PC
(if applicable)  Install the LC Diagnostic
 If ChemStation is present, verify communication to the LC Diagnostic
 If no ChemStation is present, configure LC Diagnostic
 Establish communication with the 1200 LC.

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Agilent 1100/1200
Liquid Chromatograph

Scope of Work ---- Installation and Familiarization

Checkout Preparation  For isocratic pump, fill the solvent bottle with 35% HPLC grade Water and 65% HPLC grade
Acetonitrile. For all other pumps, fill solvent bottle A with HPLC grade Water. Fill solvent bottle B with
HPLC grade Acetonitrile. If installed, purge each channel of the on-line degasser using the supplied
syringe as specified in the Degasser Reference Manual.
 Open Pump Purge Valve, and purge each channel at the maximum flow rate possible. For the binary
pump, 5ml/min, for the isocratic and quaternary pump 10ml/min. Purging each channel for 5 minutes
should be sufficient to remove air in the system.
 Install the supplied checkout column (993967-906 or 846975-202 for PS systems) into the Column
Compartment if applicable.

6.3. Checkout
The following conditions apply to the checkout of an 1100/1200 LC system.

 Checkout of a 1200 detector is applicable only to the models listed in Table 2-1. Table 6-3 defines
checkout specifications with ChemStation or Control Module control using the methods and conditions
listed in the Table 6-3 or from the Detector Reference manual. Autosampler and detector combinations
not listed in Table 6-3 or use of conditions other than those specified do not have specific limits. In those
cases, generation of a chromatogram similar to that specified in Table 6-2 will act as the final checkout.
 Checkout of a 1200 system with a third party detector will be limited to generation of a chromatogram.
No specific limits will apply. In addition, communication to a third party data system is not an Agilent
responsibility and must be handled by the Customer.
 Flush flow-cell with HPLC grade Water for 5 minutes then run the Wavelength Calibration and Intensity
Test where applicable. For an RID detector, perform the Diode balance at this time.
 Allow system to equilibrate at the below checkout conditions for 10 minutes. Prepare a vial of the Isocratic
Checkout sample*, part number - 01080-68704. Start a run using the conditions in Table 6.3.
 Special Systems, such as prep. Scale and Purification Systems and ELSD detector may require a special
checkout procedure. Please perform these procedures according to the documentation supplied for these
systems

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 Agilent 1100/1200
Liquid Chromatograph

Scope of Work ---- Installation and Familiarization

Table 6.3 Detector Checkout Specifications

Detector Method Checkout Specifications

 For Isocratic pump, fill the solvent bottle with 35% HPLC grade Water and 65%  See Below
HPLC grade Acetonitrile. For all other pumps, fill solvent bottle A with HPLC
grade Water. Fill solvent bottle B with HPLC grade Acetonitrile
All 1200 Detectors  1.5 ml/min flow-rate of 35% A and 65% B
 Inject Isocratic Checkout sample*, part number – 01080-68704
 Column – Zorbax Eclipse XDB-C8, 4.6 x 150mm, 5m, part number – 993967-
906
 Column Compartment 25C  4 peaks visible in chromatogram
 Injection volume 1l  Intensity Test – Pass
VWD/DAD/ MWD  Stop Time = 6 minutes  Wavelength Calibration  1nm
 254nm for VWD, S:254,4 and R:500,100 slit width of 4 for the DAD
 Save method as ISO-1.M
 Injection volume 1l **Make a 1:10 dilution of the Isocratic Sample in MeOH or  Chromatogram as seen in FLD
ACN Reference Manual
 Stop time = 4 Minutes  Intensity Test – Pass
FLD  Column compartment temperature = 30C  Wavelength Calibration  3nm
 FLD EX=246nm, EM=317nm
 PMT Gain=10
 FLD Response Time = 4 seconds
 Save method as ISO-1.M
 Stop time = 10 minutes  Diode Balance - 0.5
 injection volume = 20l (### µl? ###  4 peaks visible in chromatogram
RID  Column compartment and optical unit temperature = 35C
 Save method as ISO-1.M
 Flow = 1 ml/min  Equilibrate for 15 minutes
 Solvent = 80% water, 20 % acetonitrile  One Peak visible in chromatogram
ELSD  Column = Eclipse XDB-C18 4.6x150 5u Analytical
 Stop time = 15 minutes
 Sample = Caffeine standard 250 µg/ml in water, G4218-85000 (included to ELSD)
 Injection volume = 20µl
 Column compartment and optical unit temperature = 40°C
 ELSD temperature = 40 °C
 ELSD gain = 7
 ELSD filter = 1 s
 Save method as CAFFEINE.M
* The Isocratic sample does not ship with the 1200 system. The support provider must bring this sample with them to perform the checkout.

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Agilent 1100/1200
Liquid Chromatograph

Scope of Work ---- Installation and Familiarization

6.4. Familiarization

Familiarization is intended to give operators a basic overview of the operation and maintenance of new
instruments, systems, and software and is not designed to substitute for a full operator trainer course. The
customer must know how to operate a computer and be familiar with the operating system. Familiarization
does not include training on the PC, Windows, or networking.

Table 6.4.1 1200 ChemStation Bundle Familiarization

Tasks Task Details Time Notes and Objectives

1) System  Review with the customer what equipment they have and indicate the  For Systems that do not
Overview and modules that familiarization will be provided on. have a Control Module,
Hardware  Point out each Module Reference Manual. Describe the Status Indicators detailed
Familiarization using the Status Indicators section of the Module Reference Manual. 45 Minutes Familiarization of the
 Using the Agilent 1200 Series Liquid Chromatograph Familiarization 1200 set points will be
checklist, the appropriate Module Reference Manual, and the Control covered in the EDIT
Module, give an overview of the available parameters for the installed METHOD and
module(s). For this task, go to the chapter titled “Control Module DIAGNOSTIC
Screens…”. The Customer should use the Control Module to step through portions of the
the screens in this chapter while the Support Provider follows along in the ChemStation
Reference Manual while providing additional details as needed. Familiarization.

2) System Startup  Demonstrate how to turn on PC and Peripherals. Demonstrate how to log in  Understand
into the operating system and demonstrate how to change the login ChemStation File
password. Structure
 Using Windows Explorer (or another windows explorer), show the location  Configure instruments
of the Agilent ChemStation Software. Show where the instrument(s)  How to start
directories, default data, method and sequence directories are located. ChemStation
 Demonstrate the use of the ChemStation Configuration editor.
 Explain the difference between the Online and Offline Instrument sessions.
 Explain how to open a Reprocessing Copy (explain that the reprocessing
copy will start automatically switching to Data Analysis, while Data 1.5 Hour
Acquisition is running)
 Start up an Online session.

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3) General  Describe the resources available (software manuals, on-line help,  Demonstrate Online
Familiarization software support) including the support information on the CD-ROM Help and reference
such as the SSB and SRB. installation and
 Demonstrate how to use the on-line help and the tutorial. operator guides
 Explain the software layout and how to navigate between the different  Refer to the Tutorial
views using the Navigation Pane for first time users
 Explain use of the various Views
 Demonstrate how to change between the short/full menus.
 Explain ChemStation Explorer
 Add a new method and data path e.g. drive d:\test\methods and d:\test\
data using the Path Preferences and show impact on ChemStation
Explorer
 Explain Data Organization, particular the single/sequence data file
container
 Demonstrate mouse actions in the various tables and graphic items
 Explain the user access levels.
Data Storage Concepts:
 Overview about 2 ChemStation Data Packaging Concepts using
Sequence Preferences
1.Unique Folder Creation ON
(consistency of whole data set)
2.Unique Folder Creation OFF
(pure data storage)
After the short overview about the two different data storage concepts within
ChemStation ask the customer about his preference and continue to explain the
preferred mode to the customer.

4) Edit Method Configure Instruments:  Editing/Saving a

 Where necessary (e.g. for samplers and fraction collectors: Configure well method using checkout
plates and test tubes (if applicable) specs from table 6.3
For fraction collectors, only: Configure delay volume, collection order and
mode, needle movement
 Load ISO-1.M checkout method used in Section 6.3.
 Select “Edit the Entire Method” from the ChemStation menu.
 Explain the available method parameters using the on-line help and 1200
operators’ manuals for guidance.
 Don’t forget the parameters, that can be entered under the “More” and the
“Auxiliary” Settings
 If the Spectral Software (G2180BA) is loaded, select acquisition of all
spectra using the full spectral range of 190-950 and peak width that
corresponds to one spectra every 0.1 seconds.
 Save the method as ISO-1.M
5) Run Method  Place the Checkout sample prepared in Section 6.3, into vial location 1 of  Demonstrate loading
Autosampler. If an Autosampler is not available introduce the sample using and running a method.
the Manual Injection valve.
 Load ISO-1.M. 30 min
 Select Sample Info and define a data file name. Select a data path e.g. drive
d:\test\data and enter the appropriate sample information including the vial
location.
 Select Run Method.
 As data is being acquired, demonstrate the use of the instrument status
monitors and on-line signal displays available in the View menu.
 After acquisition, point out a data file specific ACQ.M and DA.M has been
created and show the location using the Windows Explorer.

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Scope of Work ---- Installation and Familiarization

6) Edit/Run  Describe the difference between a method and a sequence.  Demonstrate edit and
Sequence sequence execution
Unique Folder Creation ON
 Load sequence DEF_LC.S
 Explain usage of the sequence preference “Unique Folder Creation
ON”
 Define a sequence “Name Pattern” e.g. Operator, Date, Counter
 Demonstrate preparation of a sequence template using the “Sequence
Parameters” select new data path e.g. d:\test\data , explain the data file naming
 Continue demonstrate preparation of a sequence by opening the
“Sequence Table” menu and edit a sequence line and describe the various
fields
 Demonstrate how to save a sequence “Save Sequence”
 Load Sequence INSTPERF.S
 Demonstrate the final sequence data container (based on the sequence
pattern) and explain the content of the data container (using Windows
Explorer)
 Demonstrate “Partial Sequence” and the creation of unique data
container for each executed sequence part, based on the sequence pattern
 Explain that reprocessing of sequences using this data storage mode is
a Data Analysis task. 20 min

Unique Folder Creation OFF

 Load sequence DEF_LC.S
 Explain usage of the sequence preferences “Unique Folder Creation OFF”
 Demonstrate preparation of a sequence using the “Sequence Parameters” ,
select new data path e.g. d:\test\data , explain the data file naming
 Explain the option “Reprocessing Only”, and point out to use this function
always for reprocessing a sequence created with ChemStation versions till
B.01.03 and data created using this data storage mode.
 Continue demonstrate preparation of a sequence by opening the “Sequence
Table” menu and edit a sequence line and describe the various fields
 Run a single injection sequence using the checkout sample.
 Demonstrate how to save a sequence “Sequence Save”
 Load Sequence INSTPERF.S
 Demonstrate “Partial sequence” for this data storage mode (all data files will
be stored in the same subdirectory).
 Use Windows Explorer to show the various location of the data (no
container has been created), show that master method and sequences remain
in default directories (no copies are generated and stored along with the data)

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Scope of Work ---- Installation and Familiarization

7) Data Analysis  Select VIEW, DATA ANALYSIS.  Demonstrate

 Load data file C:\CHEM32\1\DATA\DEMO\ISOCRA.D Integration and Reports
 Demonstrate use of the ChemStation Explorer
 Explain Navigation Table and demonstrate table options, e.g. move the
column “method” to a different place in the Navigation TableDemonstrate
the various ways to load a signal and how to get signal specific information
(File Menu\Load Signal; Navigation Table\Double click)
 Demonstrate the Autointegrate feature.
 Demonstrate Graphic manipulation by selecting GRAPHICS, SIGNAL
OPTIONS. Under RANGES, select USE RANGES and enter 1.5 in the
minimum time range.
 Demonstrate Integration Events by selecting INTEGRATION,
INTEGRATION EVENTS. Demonstrate how to make changes to the two
tables and save the changes to an integration events file. Exit out of the
Integration Events table.
 Demonstrate Manual Integration by selecting INTEGRATION, DRAW
BASELINES.
 Generate an Area Percent Report by selecting REPORT and selecting the
QUALITATIVE options PERCENT, AREA, and SIGNAL. Select the
DESTINATION to be the PRINTER. Select REPORT, PRINT REPORT

For Unique Folder Creation ON:

 Load sequence data DEMO_SEQUENCE in X:\CHEM32\1\DATA\DEMO\
…using the ChemStation Explorer 45 Minutes
 Demonstrate “reviewing” of data files using the signal/review preferences
a. Use current
b. Use sequence method
c. Use method from data file
 Demonstrate “reprocessing” of data files, e.g change a multiplier by
modifying the sequence table in Data Analysis

 For Unique Folder Creation OFF

 Load sequence data BATCH in X:\CHEM32\1\DATA\DEMO\ using the
ChemStation Explorer
 Demonstrate “reviewing” of data files using the signal/review preferences
Use “Use current” and explain that
”Use sequence method” and “Use method from data file” cannot be used
(not present container, no sequence and individual methods are created in
this mode).
 Outline that reprocess for this data storage mode cannot be done in Data
Analysis
 Demonstrate to how to reprocess this data set using the “Sequence Parameter
– Reprocess only” in the Method and Run Control
 Explain the usage of “use Sequence Table information” in the “Sequence
Parameter” dialog

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Scope of Work ---- Installation and Familiarization

8) Quantification  Demonstrate the creation of simple Calibration by using the following steps;  Create Multi-Point
 Load method DEF_LC.M. Load data file ISOCRA.D. Select VWD1A only Calibration using
for this demonstration. AUTOINTEGRATE the file. stored data
 Open the INTEGRATION EVENTS TABLE and add 2 lines to the table.  Generate a Report
At TIME 0 INTEGRATION OFF. At TIME 3.5 INTEGRATION ON. Exit
and save the Integration events to the method. 45 Minutes
 Select CALIBRATION, NEW CALIBRATION TABLE. Use Automatic
Setup to add LEVEL 1, DEFAULT AMOUNT 1. In COMPOUND NAME
of the calibration table, name this Test Peak.
 Select GRAPHICS, SIGNAL OPTIONS. Under RANGES, select USE
RANGES and enter 3.5 in the minimum time.
 Save the method as TEST by selecting FILE, SAVE METHOD. Enter
TEST as the method name.
 Demonstrate the creation of an ESTD quantification report by selecting
REPORT, SPECIFY REPORT. Under QUANTATIVE RESULTS, select
Calculate ESTD, Based on AREA, Sorted by SIGNAL. Select Destination
PRINTER. Select REPORT, PRINT REPORT.
9) Spectral Data  In Data Analysis, load the Checkout data file from step 5.  Explain the detailed
Evaluation (if  Demonstrate selection of Spectra. Select the Spectral Task Icon. Using training on Spectral
applicable) either the Spectra Tool Icons or the drop down menus from the menu Evaluation is available
SPECTRA, demonstrate how to select a spectrum and reference spectrum. 1.5 Hour from Agilent. This
 Demonstrate how to display a 3D plot. Make sure that FULL MENU option will be covered in the
is selected. Under SPECTRA, select 3D PLOT. Demonstrate the Rotate Service Review
functions. Exit the 3D plot. Select NO when asked to unload the spectral Section.
data from memory.
 Demonstrate an Isoabsorbance plot. Select SPECTRA, ISOABSORBANCE
PLOT. Move the crosshairs around to demonstrate the change to the
chromatogram and spectra windows. Exit the Isoabsorbance Plot.
 Demonstrate the creation of a Spectral Library. Load method DEF_LC.M.
Load data file ISOCRA.D or load the Checkout datafile from step 5. Select
SPECTRA, LIBRARY, NEW LIBRARY. Name the library DEMO.uvl and
type in DEMO LIBRARY in the information category. Select SPECTRA,
SELECT SPECTRA, and click on the first peak. Select SPECTRA,
LIBRARY, ADD ENTRIES. Type in PEAK1 for the name. Repeat the last
3 steps for the remaining 3 peaks using PEAK2, PEAK3, PEAK4 for the
entry names. Select SPECTRA, LIBRARY, SAVE LIBRARY.
 Demonstrate the creation of an Automated library search report. Select
REPORT, AUTOMATED LIBRARY REPORT. Use the Spectral Library
File X:\CHEM32\SPECLIBS\DEMO.uvl. Select REPORT, SPECIFY
REPORT. Choose the destination PRINTER and style LIBRARY
SEARCH. Select REPORT, PRINT REPORT. Review the resulting report.
 Demonstrate Peak Purity. Load the Checkout datafile from step 5 or use
DEMODADN.D. Select SPECTRA, SELECT PEAK PURITY and click on
a peak to obtain its purity information. When prompted to update to the new
spectra/purity options click “YES”. Highlight that this decision has to be
made once per method. Use SPECTRA, SEARCH PEAKS AND PRINT to
show the automatic purity check. Explain the purity setpoints in SPECTRA,
SPECTRA OPTIONS.
10) Preparative and  Demonstrate, how to reset the tray fill information 1.5 hours  These instructions are
Purification  Demonstrate, how to perform a delay calibration valid only for systems
Systems (if  Demonstrate fraction collection in the time based and peak based mode operated with the
applicable)  Demonstrate the use of the Fraction Preview ChemStation, only
 Demonstrate the Fraction collection in a single run (method) using the  Systems with the
“Sample Info” for specifying a Fraction Start Location PURIFICATION Add-
 Generate and run a Fraction Collection Sequence with a Fraction Start On SW require a
position in the Sequence Parameters and in the Sequence Table special familiarization
 Demonstrate, how to do Pooling for the Add-On SW
 Demonstrate, how to do Sample Recovery (if applicable, appropriate trays
and configuration must be available)
 Demonstrate, how to generate a report including Fraction Tickmarks and
Fraction Table

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Agilent 1100/1200
Liquid Chromatograph

Scope of Work ---- Installation and Familiarization

11) Maintenance/ ChemStation:  Cover all Maintenance

Diagnostics  Discuss Computer maintenance issues including file cleanup, defrag and issues including file
scandisk 25 Minutes clean up, defrag and
Use for  Discuss the importance of regular backups. scandisk.
1200 Series LC  Specify to customer all consumables needed to run the instrument using the
Modules. consumable catalogs for guidance.
 Using each installed Module Reference Manual, point out the “Simple
See section 12 for SL Repair” section in each manual.
modules  For Pumps, review replacement of valves, frits, and seals, filters and
seal wash parts (if applicable)
 For Autosamplers, review replacement of needle and needle seat, rotor,
plunger seal (+ sample loop and wash port parts, where applicable)
 For detectors, review replacement of lamps, cleaning and flushing of
flow cell
 For fraction collectors review replacement of tubings
 For valves review replacement of rotor and rotor seal (if applicable)
 Select VIEW, DIAGNOSIS. Select DIAGNOSIS, TESTS. Review the
available tests. Select CLOSE to exit.

LC Diagnostic:
 Describe Guided Diagnostic
 Describe Status Report and how to generate a report as PDF or as a printable
file

12) LC Diagnostic  Describe Guided Diagnostic  Cover all Maintenance

 Describe Status Report and how to generate a report as PDF or as a printable issues.
file 25 Minutes
Use for  Overview about existing diagnostic tasks
1200 Series  Calibration procedures
Rapid Resolution SL  Additional maintenance procedures, e.g. injector steps
LC Systems  EMF editor including parameter limits

13) Service Review  Refer to Section 7.0 of this Document 30

Minutes

Table 6.4.2 1200 Standalone Module/Bundle Familiarization

Tasks Task Details Time Notes and Objectives

1) System  Review with the customer what equipment they have and point out each  The Module Reference
Overview and Module Reference Manual. Describe the Status Indicators using the Status Manuals will be
Hardware Indicators section of the Module Reference Manual. needed for the
Familiarization  Using the Agilent 1200 Series Liquid Chromatograph Familiarization 45 Minutes Familiarization.
checklist, the appropriate Module Reference Manual, and the Control
Module, give an overview of the available parameters for the installed
module(s). For this task, go to the chapter titled “Control Module
Screens…”. The Customer should use the Control Module to step through
the screens in this chapter while the Support Provider follows along in the
Reference Manual while providing additional details as needed.

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Agilent 1100/1200
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Scope of Work ---- Installation and Familiarization

2) Maintenance  Specify to customer all consumables needed to run the instrument using the 
consumable catalogs for guidance.
 Using each installed Module Reference Manual, point out the “Simple 15 Minutes
Repair” section in each manual.
 For Pumps, review replacement of valves, frits, and seals, filters and
seal wash parts (if applicable)
 For Autosamplers, review replacement of needle and needle seat, rotor,
plunger seal (+ sample loop and wash port parts, where applicable)
 For detectors, review replacement of lamps, cleaning and flushing of
flow cell
 For fraction collectors review replacement of tubings
 For valves review replacement of rotor and rotor seal (if applicable)

3) Service Review  Refer to Section 7.0 of this Document 30 

Minutes

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Agilent 1100/1200
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Scope of Work ---- Installation and Familiarization

7. Service Review
Review with the customer all bullet items listed. Typical time required for service review takes 30 minutes.
 Complete the Attendees list
 If applicable enroll the customer for 1 year Software Support.
 Attach all reports, chromatograms to the Checklist for future reference.
 If available, record in a Customer provided instrument logbook the date and time installation is completed.
 Explain the importance of license numbers to the customer and that he/she must store them securely.
 Explain hardware warranty, software warranty and return policy for Agilent Instrument Service Center.
 Describe how to create a Service Call
 Provide customer with a schedule of CAG Support Services. Reference Publication number 5980-0281EE.
 Briefly explain the use of all Operator and Reference Manuals.
 Inform customer of additional training classes.
 If the customer has an Internet connection, offer to show these Agilent Technologies external web-sites.

Technical Support
http://www.chem.agilent.com/Scripts/cag_techsupport.asp

Library
http://www.chem.agilent.com/Scripts/Library.asp

Education
http://www.chem.agilent.com/Scripts/cag_countrysites.asp?pf=T

E-seminars
http://webshop.chem.agilent.com/iccdocs/seminarList.shtml

Supplies
http://www.chem.agilent.com/chem/go

Software Status Bulletins and Patches

http://www.chem.agilent.com/chem/go

 Complete Sales Order, CSO Number, Date, Customer signature and Support Provider signature

*01200-90605* Part Number: 01200-90605 © Agilent Technologies, Inc. 2008

*01200-90605*
01200-90605 EditionSeptember/ 2008 Agilent Technologies
Printed in Germany Hewlett-Packard-Strasse 8
76337 Waldbronn, Germany
*01200-90605*
Issued: September 2008 – Revision 5.2 Part Number:
Agilent 01200-90605
Restricted © Agilent
WAD Product Support Technologies,
Page 1Inc.
of 2008
17

*01200-90605*
01200-90605 Edition 02 / 2008 Agilent Technologies
Printed in Germany Hewlett-Packard-Strasse 8