Professional Documents
Culture Documents
Liquid Chromatograph
Familiarization Checklist
Purpose of Procedure
To demonstrate the steps required to perform a basic analysis using a standard sample or internal data file, evaluate
the resulting data and perform routine operational maintenance. Familiarization is intended to give operators a basic
overview of the operation and maintenance of new instruments, systems and application software and is not
designed to substitute for a full operator-training course.
Customer Responsibilities
The customer should ensure that necessary operating supplies, consumables and usage dependent items such as
vials, syringes and solvents are available. Users of the instrument should be present at all times during the
familiarization, otherwise important information on operation and maintenance may be missed. The manuals
delivered with the instrument will be used as a guide during familiarization and should be available.
If customer did not purchase familiarization time for the 1200, check that section as not applicable
Issued: Sep 2009 – Rev. 6.3 Copyright 2009 Agilent Technologies WAD Product Support
Page 11 of 11
Agilent 1100/1200 Series
Liquid Chromatograph
Familiarization Checklist
Hardware Familiarization
Section Not Applicable
Note:
If one section contains multiple choices for installed Hardware please encircle all modules that are part of the
system and enter the corresponding Serial Numbers.
Power on each module and describe the various states of the status indicators.
Indicate the installed modules in the following list that will be covered as part of the familiarization
G4208A- Instant Pilot
G1323A/B
G1322A/79A/B- Degasser
G1310A/11A/12A /61A/76A/ G2226A - Pump
G1328A/B – Manual Injector
5065-9922 – Prep. Manual Injector
G1313A/29A/B, G1389A or G2260A – Autosampler
G1367A/B/C/D/77A – Well Plate Sampler
G2258A – Dual Loop Sampler
G1316A/B/C - Column Compartment
G1157A/58A/59A/60A/62A/63A – Valves
G1314A/B/C/D/E – VWD
G1315A/B/C/D-DAD / G1365A/B/C/D–MWD
G1321A – FLD
G1362A – RID
G4218A – ELSD
G1364A/B/C/D – Fraction Collectors
G1330A/B – Thermostat for Samplers and Fraction Collectors
Demonstrate stand-alone operation for each installed Module using the Control Module. Use the “Control
Module Screens for the 1100/1200” section from the appropriate Module Reference Manual as guidance.
Review the following Screens:
1. Views
2. Diagnostics and Tests Screen
3. G4218A ELSD: Demonstrate use of built-in keyboard control for using the instrument
Explain installation and safety features of the instrument/system. For the G4218A ELSD: explain the
importance of setting the right nitrogen pressure, using the exhaust line, avoiding pressure fluctuations,
avoiding leaks and monitoring the liquid level in the siphon.
Issued: Sep 2009 – Rev. 6.3 Copyright 2009 Agilent Technologies WAD Product Support
Page 11 of 11
Agilent 1100/1200 Series
Liquid Chromatograph
Familiarization Checklist
Maintenance/Diagnostics
Review common maintenance procedures by reviewing the “Simple Repairs” section in the appropriate
Reference Manual
G4218A ELSD: Explain no self-repair of internal parts of the ELSD is possible for safety reasons.
Show the use of diagnostic utility (LMD/LA) further information included in manuals and this document.
Explain need for regular maintenance, cleaning and appropriate storing of instruments, and for the G4218A
ELSD, the need of regularly cleaning the nebulizer (Please note: if ELSD is not stopped/cleaned appropriately
after use, this will significantly decrease the nebulizer/detector life time. Similar comment on flushing
capillaries etc.
Explain need for avoiding growth of algae within the system which can create problems and high costs for
customers as this is not covered by the warranty or support contract (Customer is liable for repair charges).
For Systems that do not have a Control Module, detailed Familiarization of the 1200 set points will
be covered in the EDIT METHOD and DIAGNOSTIC portions of the Software Familiarization.
Issued: Sep 2009 – Rev. 6.3 Copyright 2009 Agilent Technologies WAD Product Support
Page 11 of 11
Agilent 1100/1200 Series
Liquid Chromatograph
Familiarization Checklist
Software Familiarization
Section Not Applicable
System Startup
Demonstrate how to turn on PC and Peripherals. Demonstrate how to log in into the operating system and
demonstrate how to change the login password.
Advise customer on back-up and restoration procedures with Agilent Backup Software. Refer customer to
product manual.
Using Windows Explorer (or another windows explorer), show the location of the Agilent ChemStation
Software. Show where the instrument(s) directories, default data, method and sequence directories are located.
Demonstrate how to start Configuration Assistant.
Demonstrate the use of the Configuration Assistant. Explain the impact of configuring instruments as “Modular
LC” or “Modular LC (classic)” on ChemStation UI, method migration, Diagnosis view etc. Point out the
ChemStation Upgrade Preparation Guide for more detailed explanation.
Demonstrate the use of the ChemStation Configuration editor.
Explain the difference between the Online and Offline Instrument sessions.
Explain how to open a Reprocessing Copy (explain that the reprocessing copy will start automatically
switching to Data Analysis, while Data Acquisition is running).
Start up an Online session.
General Familiarization
Describe the resources available (software manuals, on-line help, software support) including the support
information on the CD-ROM such as the SSB and SRB.
Demonstrate how to use the on-line help and the tutorial.
Explain the software layout and how to navigate between the different views using the Navigation Pane
Explain use of the various Views
Demonstrate how to change between the short/full menus.
Explain ChemStation Explorer
Add a new method and data path e.g. drive d:\test\methods and d:\test\data using the Path Preferences and show
impact on ChemStation Explorer
Explain Data Organization, particular the single/sequence data file container
Demonstrate mouse actions in the various tables and graphic items
Explain the user access levels.
Data Storage Concepts:
Overview about 2 ChemStation Data Packaging Concepts using Sequence Preferences
1.Unique Folder Creation ON
(consistency of whole data set)
2.Unique Folder Creation OFF
(pure data storage)
After the short overview about the two different data storage concepts within ChemStation ask the customer about
his preference and continue to explain the preferred mode to the customer, only.
Configure Instruments
Where necessary (e.g. for samplers and fraction collectors: Configure well plates and test tubes (if applicable)
For fraction collectors, only: Configure delay volume, collection order and mode, needle movement
Issued: Sep 2009 – Rev. 6.3 Copyright 2009 Agilent Technologies WAD Product Support
Page 11 of 11
Agilent 1100/1200 Series
Liquid Chromatograph
Familiarization Checklist
Edit Method
Load ISO-1.M checkout method used in Section 6.3.
Select “Edit the Entire Method” from the ChemStation menu.
Explain the available method parameters using the on-line help and 1200 operators’ manuals for guidance.
Demonstrate how to access instrument parameters via the System Diagram.
Don’t forget the parameters, that can be entered under the “More” and the “Auxiliary” Settings
If the Spectral Software (G2180BA) is loaded, select acquisition of all spectra using the full spectral
range of 190-950 and peak width that corresponds to one spectra every 0.1 seconds.
Save the method as ISO-1.M
Demonstrate method migration by loading method BATCH.M and saving it.
Explain method resolution.
Run Method
Place the Checkout sample prepared in Section 6.3, into vial location 1 of Autosampler. If an Autosampler is
not available introduce the sample using the Manual Injection valve.
Load ISO-1.M.
Select Sample Info and define a data file name. Select a data path e.g. drive d:\test\data and enter the
appropriate sample information including the vial location.
Select Run Method.
As data is being acquired, demonstrate the use of the instrument status monitors and on-line signal displays
available in the View menu.
After acquisition, point out a data file specific ACQ.M and DA.M has been created and show the location using the
Windows Explorer.
Issued: Sep 2009 – Rev. 6.3 Copyright 2009 Agilent Technologies WAD Product Support
Page 11 of 11
Agilent 1100/1200 Series
Liquid Chromatograph
Familiarization Checklist
After the short overview about the two different sequence options ask the customer about their preference and
continue to explain the preferred mode to the customer, only.
Issued: Sep 2009 – Rev. 6.3 Copyright 2009 Agilent Technologies WAD Product Support
Page 11 of 11
Agilent 1100/1200 Series
Liquid Chromatograph
Familiarization Checklist
Issued: Sep 2009 – Rev. 6.3 Copyright 2009 Agilent Technologies WAD Product Support
Page 11 of 11
Agilent 1100/1200 Series
Liquid Chromatograph
Familiarization Checklist
Data Analysis
Demonstrate use of the navigation pane
Explain navigation table and demonstrate table options, e.g. move column “method name” to different place
Demonstrate the various ways to load a signal and how to get signal specific information (File Menu\Load
Signal;
Navigation Table\Double click)
Load data file ISOCRA.D found in X:\CHEM32\1\DATA\DEMO\
Manipulate graphics windows (Zoom, print window, etc.)
Integrate signal (Automated and Manual)
Setup report using “Specify Report” menu
Print an area percent report
Quantification
Load method DEF_LC.M
Load data file ISOCRA.D
Demonstrate the steps in creating a single level calibration
Demonstrate generating a External Standards report
Issued: Sep 2009 – Rev. 6.3 Copyright 2009 Agilent Technologies WAD Product Support
Page 11 of 11
Agilent 1100/1200 Series
Liquid Chromatograph
Familiarization Checklist
Issued: Sep 2009 – Rev. 6.3 Copyright 2009 Agilent Technologies WAD Product Support
Page 11 of 11
Agilent 1100/1200 Series
Liquid Chromatograph
Familiarization Checklist
Maintenance/Diagnostics
Names
Comments:
Issued: Sep 2009 – Rev. 6.3 Copyright 2009 Agilent Technologies WAD Product Support
Page 11 of 11
Agilent 1100/1200 Series
Liquid Chromatograph
Familiarization Checklist
Service Review
____________________________
Customer Service Order (CSO) Number
____________________________
Date completed
____________________________
Customer Signature
____________________________
Support Provider Signature
____________________________
*01200-90604*
01200-90604 Edition September / 2009 Agilent Technologies
Printed in Germany Hewlett-Packard-Strasse 8
76337 Waldbronn, Germany
Issued: Sep 2009 – Rev. 6.3 Copyright 2009 Agilent Technologies WAD Product Support
Page 11 of 11