Pilot project: Supporting Model based iinvestigations in the classroom

Northlands Nordelta- Universität Bielefeld, Germany

Worksheet 6: Enzymes EXERCISES

Bibliography
● Chapter 3 IGCSE Biology, Mary Jones, Heinemann
● https://www.s-cool.co.uk/gcse/biology/enzymes/revise-it/enzymes
● https://www.bbc.co.uk/bitesize/guides/z9jrng8/revision/2

Part 1 (Comprehension questions)

1. Based on the following phrase, choose the correct answer: “A protein molecule which is denatured
means that it has…
(a) split into smaller molecules”.
(b) changed its shape”.
(c) combined with another molecule” .
(d) been diluted”.
2. Select the most appropriate words from the list below to complete the paragraph below.

substances – proteins – enzymes – catalysts – speed up - use up – slow down

All cells contain ……………….……… which are ……………….……… and act as ……………….
……… which ……………….……… chemical reactions.
The reactions do not ……………….……… the ……………….……… which can take part in further
reactions.

3. Enzymes will usually react with only one substance. This can be explained by the 'lock and key'
theory. If this theory is correct, which of the following substances, represented by P, Q, R and S
would be acted on by enzyme A?

4. Based on the following phrase, choose the correct answer: “If an enzyme-controlled reaction
normally takes place at 10ºC, in general terms how will the reaction be affected by…
(a) a fall in temperature to 2°C”.
(b) a rise in temperature to 20°C”.
(c) a rise in temperature to 65°C”.

5. If an enzyme is denatured, why does it no longer work? Explain your answer.

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6. The graph shows the rate of reaction of 3 different enzymes at different levels of acidity or alkalinity
(pH). Based on the information provided by the graph, what is the optimum pH for Pepsin and Trypsin
enzymes?

(a) pH 2 and 7, respectively.

(b) pH 2 and 9, respectively.
(c) pH 7 and 2, respectively.
(d) pH 7 and 9, respectively.
(e) pH 9 and 2, respectively.
(f) pH 9 and 7, respectively.

7. A protein-digesting enzyme when mixed with starch solution would

(a) have no action
(b) produce amino acids
(c) produce glucose
(d) digest the starch?

Justify your choice.

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8. Select the most appropriate words from the list below to complete the following
paragraph.

animals – extra-cellular – intra-cellular – cells – digestive – nuclei – catalysts

All enzymes are produced inside ……………….……… Enzymes which do their work outside cells are
called ……………….……… Enzymes which do their work inside cells are called ……………….………
Most of our digestive enzymes are examples of ……………….……… enzymes.

9. Describe what does the enzyme catalase do.

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10. Substance A is being investigated to see if it is an enzyme. When substance A is mixed with
substance B a reaction takes place. A control experiment is conducted using a sample of A which has
been boiled.
(a) Why is boiling used as a control?
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(b) If the reaction still worked after A had been boiled, what might be your interpretation?
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 Part 2 – (Past paper questions)

11. Fig. 4.2 shows a graph of the activity of two enzymes A and B from the alimentary canal.

(a) Describe the effect of changes in pH on the activity of enzyme B.

Use data from Fig. 4.2 to support your answer. [2]
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(b) The stomach produces hydrochloric acid to kill bacteria in food.

Identify which enzyme A or B works best in the stomach. [1]
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Explain your answer. [2]

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12. Fig. 7.1 shows a graph of activity against temperature for two enzymes, A and B.

(i) Identify the temperature of maximum activity of enzyme B. [1]

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(ii) The enzyme activity of both enzymes is the same at 47 °C. The temperature is increased to 50 °C.
State and explain the effects of this temperature increase on the activity of enzyme A [3]
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13
a) Design and complete a table to record the results shown in Fig 1.3. [4]
(If core, refer to the teacher for the table designing)

b) Describe and explain using the results from (a), the effect of surface area on the volume of
oxygen gas produced. [3]
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c) The student used a 25 ml measuring cylinder to collect the gas in the practice experiment. The
practice volume of oxygen gas recorded was 2.5ml. Suggest why the student then chose to use a
10ml measuring cylinder for the rest of the investigation. [1]
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d) Another student wanted to investigate the amount of catalase present in different food plants.
Describe a method that the student could use to carry out this new investigation. [5]
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