Name: ......................................................................................

Date: .........................................

Class: ........................................

IGCSE BIOLOGY EDEXCEL 9-1

CHAPTER WORKBOOK

Enzymes

Image: Thomas Shafee https://commons.wikimedia.org/w/index.php?curid=45801894 (CC BY 4.0)

Enzymes
1. Define “enzyme”.

...............................................................................................................................................
...............................................................................................................................................

2. In the space below draw a diagram to outline the lock and key hypothesis.
Include the below words as labels.

Enzyme Substrate Enzyme-substrate complex

Active site Products

Ó A. Nixon 2022. All rights reserved.

ScienceSauceOnline.com Page 2 of 4
3. The graph below shows how the rate of an enzyme-catalysed reaction
changes over time.

a) Explain the rate of reaction at points A, B and C.

……………………………………………………………………..
A ……………………………………………………………………..

……………………………………………………………………..

……………………………………………………………………..
B ……………………………………………………………………..
……………………………………………………………………..

……………………………………………………………………..
C ……………………………………………………………………..

……………………………………………………………………..

b) In terms of the active site state what happens when an enzyme becomes
denatured.
...............................................................................................................................................

c) The enzyme presented in question 3 is an enzyme found in the human

digestive system. State and explain what the temperature is likely to be at
point B.
...............................................................................................................................................
...............................................................................................................................................
...............................................................................................................................................
...............................................................................................................................................

Ó A. Nixon 2022. All rights reserved.

ScienceSauceOnline.com Page 3 of 4
 d) Explain the effect of pH on the rate of an enzyme-catalysed reaction.

...............................................................................................................................................
...............................................................................................................................................
...............................................................................................................................................
...............................................................................................................................................

Ó A. Nixon 2022. All rights reserved.

ScienceSauceOnline.com Page 4 of 4