Work Sheet

Analysis of Lipids

Name:___Campomanes, Elspeth Tamar H.______

OBJECTIVES:
1. To test the solubility of lipid samples in some solvents
2. To test the nonpolar behavior of lipid samples
3. To investigate the chemical behavior of lipid-containing samples such as cooking oil, vitamin E, and egg yolk
4. To make soap by alkaline hydrolysis

Data and Results

A. Solubility Test
Solvent Coconut oil* Vit. E* Beef fat/tallow *
Hexane Miscible Miscible Miscible
Ethyl acetate Miscible Miscible Miscible
Methanol Immiscible Immiscible Immiscible
Water Immiscible Immiscible Immiscible
Note: Miscible or immiscible

Sudan Test
Solvent Coconut oil* Vit. E* Beef fat/tallow *
Hexane Homogeneous Homogeneous Homogeneous
Ethyl acetate Homogeneous Homogeneous Homogeneous
Methanol Heterogeneous Heterogeneous Heterogeneous
Water Heterogeneous Heterogeneous Heterogeneous
Note: Homogeneous or heterogeneous

B. Bromine Test
Sample Number of Drops of Bromine * Degree of Unsaturation **
Oleic acid 3rd Highest
Coconut oil 2nd Moderate
Olive oil 1st Minimal
* Rank (1 , 2 , 3 ) as to the estimated number of drops of bromine used: 1 – highest number of drops and 3rd – lowest
st nd rd st

number of drops
** Minimal, moderate, highest

C. Acrolein Test
Sample Observation* Remarks**
Coconut oil Pungent odor Positive
Egg yolk Pungent odor Positive
Oleic acid Pungent odor Positive
Beef fat/tallow Pungent odor Positive
* Pungent odor or no observable result
** positive or negative for glycerol

D. Zak’s Test
Sample Observation* Remarks**
Oleic acid No observable result Negative for cholesterol
Coconut oil No observable result Negative for cholesterol
Egg yolk Purple product Positive for cholesterol
* Purple product or no observable result
** positive or negative for cholesterol

E. Saponification

Sample Remarks *
st
Soap suspension (Step 2) 1
Purified Soap (Step 3) 2nd
Acid hydrolysis product of soap (Step 4) 3rd
* Rank (1 , 2 , 3 ) as to the estimated pH value: 1 - highest pH value and 3rd lowest pH value
st nd rd st